Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,046,652 Δ1 bp 2.0% coding (31/1239 nt) RCAP_rcc02856 ← PAS/PAC sensor domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,046,6520G→. 2.0% 11.3 ‑1.3 ‑0.0348coding (31/1239 nt)RCAP_rcc02856PAS/PAC sensor domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (5/2):  ref base (167/174):  total (172/176)
Fisher's exact test strand distribution p-value = 2.79e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.61e-01

Only 100 of 432 total aligned reads displayed.

GAGACGCGCCGGAAGACCTCGTTGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGCCCCAGCCTAACCGCGGGATCGTT  >  NC_014034/3046552‑3046752
                                                                                                    |                                                                                                    
gagaCGCGCCGGAAGACCTCGTTGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCg                                                                                                      >  2:6278163/1‑101 (MQ=255)
        ccGGAAGACCTCGTTGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCggggggggg                                                                                              >  1:7754959/1‑101 (MQ=255)
         cGGAAGACCTCGTTGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGa                                                                                             <  2:1637152/101‑1 (MQ=255)
          ggAAGACCTCGTTGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCggggggggggg                                                                                            >  2:3468493/1‑99 (MQ=255)
          ggAAGACCTCGTTGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAg                                                                                            >  2:2689872/1‑101 (MQ=255)
           gAAGACCTCGTTGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGGgg                                                                                           >  2:5553009/1‑101 (MQ=255)
            aaGACCTCGTTGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAggg                                                                                          >  2:1876074/1‑100 (MQ=255)
            aaGACCTCGTTGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGt                                                                                          <  1:1189562/101‑1 (MQ=255)
              gACCTCGTTGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCg                                                                                        <  1:900888/101‑1 (MQ=255)
               aCCTCGTTGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCgg                                                                                       <  2:3611357/101‑1 (MQ=255)
                  tCGTTGCCGGACAGGACCCCGCCGGGGGGGTCGGTGCGCGAAAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCCCCCTCCCGGGGGGGGGAGGTCGGACa                                                                                    <  1:1445566/101‑1 (MQ=255)
                   cGTTGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAg                                                                                   <  2:6465146/101‑1 (MQ=255)
                   cGTTGCCGGACAGGATCACGCCGCGGGGGTCGGCGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGGCGGACAg                                                                                   >  1:404563/1‑101 (MQ=255)
                   cGTTGCCGGACAGGATCACGCCGCGGGGGGCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAg                                                                                   <  1:3838315/101‑1 (MQ=255)
                    gTTGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGGGGTCGGACAGa                                                                                  >  1:1029655/1‑101 (MQ=255)
                     ttGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGGGGGCGGACAGAc                                                                                 >  2:1480323/1‑101 (MQ=255)
                     ttGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGAc                                                                                 <  2:7997226/101‑1 (MQ=255)
                      tGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGGGGTCGGACAGACt                                                                                >  2:4695020/1‑101 (MQ=255)
                      tGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACt                                                                                <  2:8520633/101‑1 (MQ=255)
                      tGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACt                                                                                <  1:7868073/101‑1 (MQ=255)
                      tGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACt                                                                                <  1:2506551/101‑1 (MQ=255)
                       gCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGCGGTCGGACAGACTg                                                                               >  2:4352323/1‑101 (MQ=255)
                         cGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCg                                                                             <  2:4487630/101‑1 (MQ=255)
                           gACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGtt                                                                           <  1:2637334/101‑1 (MQ=255)
                           gACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCCCCCGGGGGGGGGGGGGCGGACAGAGTGCGtt                                                                           >  1:2913258/1‑101 (MQ=255)
                            ccAGGATCACGCGGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTc                                                                          <  2:8964737/100‑1 (MQ=255)
                            aCAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTc                                                                          <  1:8009926/101‑1 (MQ=255)
                              aGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCAt                                                                        >  1:6416065/1‑101 (MQ=255)
                              aGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCAt                                                                        <  2:5722437/101‑1 (MQ=255)
                              aGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCAt                                                                        >  1:6158134/1‑101 (MQ=255)
                                 aTCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCgg                                                                     >  1:3182026/1‑101 (MQ=255)
                                 aTCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCgg                                                                     >  1:8533739/1‑101 (MQ=255)
                                  tCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGGGGTCGGACAGACTGCGTTCATCggg                                                                    >  1:7283028/1‑101 (MQ=255)
                                    aCGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGc                                                                  <  2:6083921/101‑1 (MQ=255)
                                      gccgcGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGGGGTCGGGCAGACTGCGTTCATCGGGGctc                                                                >  2:6620914/1‑101 (MQ=255)
                                         gcgGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCg                                                             <  1:5698449/101‑1 (MQ=255)
                                         gcgGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCg                                                             <  1:5259716/101‑1 (MQ=255)
                                          cgGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGt                                                            >  1:821627/1‑101 (MQ=255)
                                          cgGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGGCGGACAGACTGCGTTCATCGGGGCTCTCGt                                                            >  1:6201986/1‑101 (MQ=255)
                                           gggggTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGtt                                                           >  1:209194/1‑101 (MQ=255)
                                           gggggTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGGCGGACAGACTGCGTTCATCGGGGCTCTCGtt                                                           >  2:3050645/1‑101 (MQ=255)
                                           gggggTCGGGTCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGtt                                                           <  2:7230387/101‑1 (MQ=255)
                                            ggggTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGGGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTg                                                          >  2:6844122/1‑101 (MQ=255)
                                            ggggTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTg                                                          >  2:8699385/1‑101 (MQ=255)
                                             gggTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTgg                                                         >  1:5806068/1‑100 (MQ=255)
                                              ggTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAg                                                        >  1:9154993/1‑101 (MQ=255)
                                              ggTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGGTCATCGGGGCTCTCGTTGAg                                                        >  2:2366766/1‑101 (MQ=255)
                                               gTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCC.GGGGGGGGAGGTCGGACNNACNGCGTTCATCGGGGCTCTCGTTGAGTg                                                      >  1:4521898/1‑101 (MQ=255)
                                                tCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTg                                                      >  2:5285474/1‑101 (MQ=255)
                                                tCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTg                                                      >  1:3050077/1‑101 (MQ=255)
                                                 cGGTGCTCGAGAAGAAAAGCTCATGAAAGCCGAAAGGGGATTCTCCCTCCCGGGGGGGGGAGGGCGGAAGGACTGGGTTCACCGGGGGCCTCggtagttgg                                                     >  2:5106463/1‑93 (MQ=255)
                                                 cGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGGGGGCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGttg                                                     >  2:155941/1‑99 (MQ=255)
                                                  ggTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTACCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGGggg                                                    >  2:2596006/1‑101 (MQ=255)
                                                   gTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGGCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGgga                                                   >  1:1794514/1‑101 (MQ=255)
                                                     gcgcGAGAAGAAAATCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGggagg                                                 <  1:923556/101‑1 (MQ=255)
                                                      cgcgAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGggagga                                                <  1:5582561/101‑1 (MQ=255)
                                                        cgAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGGGGGaggagg                                              >  2:222519/1‑101 (MQ=255)
                                                           gaagaaAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTc                                           <  1:3651979/101‑1 (MQ=255)
                                                            aagaaAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCg                                          <  2:7633345/101‑1 (MQ=255)
                                                               aaaaGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGt                                       >  1:5028209/1‑101 (MQ=255)
                                                               aaaaGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGt                                       >  1:3409856/1‑101 (MQ=255)
                                                                aaaGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGTAGGGTCGAGTg                                      >  2:3960343/1‑101 (MQ=255)
                                                                 aaGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGc                                     >  1:1482636/1‑101 (MQ=255)
                                                                 aaGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGGCGAGTGc                                     >  2:4553305/1‑101 (MQ=255)
                                                                 aaGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACCGCGTTCATCGGGGCTCTCGTTGTGTGGGCGGAGGGTCGAGTGc                                     >  2:8595396/1‑101 (MQ=255)
                                                                  aGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCg                                    <  1:350101/101‑1 (MQ=255)
                                                                   gCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGGGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGGGGGAGGAGGGTCGAGGGCgg                                   >  2:8818263/1‑101 (MQ=255)
                                                                    cTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCggt                                  <  1:5385221/101‑1 (MQ=255)
                                                                    cTCATGAAACCCGAAATCGGCTCCTCCCCCCGGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCggt                                  <  2:6946710/101‑1 (MQ=255)
                                                                     tCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCggtg                                 <  1:5208082/101‑1 (MQ=255)
                                                                      cATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCggtgg                                >  1:7471408/1‑101 (MQ=255)
                                                                        tGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCggtgggg                              >  1:4646588/1‑100 (MQ=255)
                                                                         gAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCggtgggtg                             >  2:4132487/1‑101 (MQ=255)
                                                                          aaaGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGgtgggtgg                            >  2:3125958/1‑101 (MQ=255)
                                                                            aGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGgtgggtgggg                          >  1:4787933/1‑99 (MQ=255)
                                                                            aGCCGAAATCGGCTTAGCCCGACCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGgtggtg                          <  1:1618431/101‑1 (MQ=255)
                                                                                gAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGGCGAGTGCGGTGGGGGGTGcccc                      >  2:8262167/1‑101 (MQ=255)
                                                                                 aaaTCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGGGGGTCGAGTGCGGTGGGGGGTGCCCCa                     >  1:7134779/1‑101 (MQ=255)
                                                                                 aaaTCGGCTTCTACCTCCCGGGGGGGGGGGGTCGGAAAGACTGCGTTCATCGGGGCTGTCGTTGAGTGGGAGGAGGGCCGCATGAGGTGGGGGGGGccccc                     >  1:6602085/1‑100 (MQ=255)
                                                                                  aaTCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGCCCCAg                    <  1:5956813/101‑1 (MQ=255)
                                                                                  aaTCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGGGGTGcccccg                    >  1:217064/1‑99 (MQ=255)
                                                                                    tCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGGGTGCGGTGGGAGGGGCCCCAGcc                  >  2:5937605/1‑101 (MQ=255)
                                                                                     cGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGCCCCAGCCt                 >  1:3283531/1‑101 (MQ=255)
                                                                                     cGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGCCCCAGCCt                 <  1:6765217/101‑1 (MQ=255)
                                                                                     cGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGGGCCCCAGCCt                 >  1:2318946/1‑101 (MQ=255)
                                                                                     cGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGGGGTGCCCCAGCCt                 >  1:6815673/1‑101 (MQ=255)
                                                                                         ttgcccctcccGGGGGGGGGAGGTCGGGCAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGCCCCAGCCTAAcc             <  2:912531/97‑1 (MQ=255)
                                                                                         ttCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGGTCTCGTTGAGTGGGAGGGGGGTCGAGGGCGGTGGGGGGGGCCCCAGCCTAAcc             >  1:6713283/1‑101 (MQ=255)
                                                                                         ttCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGCCCCAGCCTAAcc             >  1:5089974/1‑101 (MQ=255)
                                                                                          tctcCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGCCCCAGCCTAAccc            >  2:5365588/1‑100 (MQ=255)
                                                                                          tctcCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGGGCCCCAGCCTAACcg            >  1:4275452/1‑101 (MQ=255)
                                                                                            tccctcccGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGCCCCAGCCTAACcgcg          <  2:7469932/101‑1 (MQ=255)
                                                                                              ccctccGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGCCCCAGCCTAACCGCggg        <  2:4233866/97‑1 (MQ=255)
                                                                                                tcccGGGGGGGTGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGCCCCAGCCTAACCGCGGGAt      <  2:1017912/101‑1 (MQ=255)
                                                                                                tcccGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGCCCCAGCCTAACCGCggggt      >  2:3304020/1‑99 (MQ=255)
                                                                                                tcccGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGCCCCAGCCTAACCGCGGGAt      <  1:1893159/101‑1 (MQ=255)
                                                                                                 cccGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGGCCCAGCCTAACCGCGGGAta     >  1:8818202/1‑100 (MQ=255)
                                                                                                    gggggggggAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGCCCCAGCCTAACCGCGGGATCGtt  >  2:7856959/1‑101 (MQ=255)
                                                                                                    gggggggggAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGCCCCAGCCTAACCGCGGGATCGtt  >  2:2071542/1‑101 (MQ=255)
                                                                                                    gggggggggAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGCCCCAGCCTAACCGCGGGATCGtt  >  2:4932762/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GAGACGCGCCGGAAGACCTCGTTGCCGGACAGGATCACGCCGCGGGGGTCGGTGCGCGAGAAGAAAAGCTCATGAAAGCCGAAATCGGCTTCTCCCTCCCGGGGGGGGGAGGTCGGACAGACTGCGTTCATCGGGGCTCTCGTTGAGTGGGAGGAGGGTCGAGTGCGGTGGGTGGTGCCCCAGCCTAACCGCGGGATCGTT  >  NC_014034/3046552‑3046752

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG