Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,791 Δ1 bp 2.9% intergenic (‑94/+69) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7910G→. 2.9% 20.2 ‑2.1 0.0375intergenic (‑94/+69)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (3/8):  ref base (197/159):  total (208/167)
Fisher's exact test strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 469 total aligned reads displayed.

GGCTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCG  >  NC_014034/3439691‑3439893
                                                                                                     |                                                                                                          
ggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAg                                                                                                            <  1:6795942/101‑1 (MQ=255)
 gcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAgg                                                                                                           <  2:2503381/101‑1 (MQ=255)
   tCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTGGGGGAAGATA.AAaggg                                                                                                         >  1:9059745/1‑101 (MQ=255)
    cATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGAAGATA.AAggggg                                                                                                        >  1:8235180/1‑100 (MQ=255)
     aTGGCGGCGACCCTACACGGCGCGACCATCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGgct                                                                                                       <  1:7958100/101‑1 (MQ=255)
       ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGgcttt                                                                                                     <  2:8435879/101‑1 (MQ=255)
       ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGgcttt                                                                                                     <  1:2813669/101‑1 (MQ=255)
       ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGgcttt                                                                                                     <  2:5777704/101‑1 (MQ=255)
         cggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGgctttgc                                                                                                   <  1:716876/101‑1 (MQ=255)
          ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGgctttgct                                                                                                  <  2:863565/101‑1 (MQ=255)
            cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGgctttgcttt                                                                                                <  1:3666361/101‑1 (MQ=255)
             gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATA.AAGGGGGTTTGGtttt                                                                                               >  2:7897683/1‑101 (MQ=255)
               cccTACAGCGCGCGACACGCGTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTAt                                                                                             <  2:2972968/101‑1 (MQ=37)
               cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTGGGGGAAGATA.AAAGGGCTTTGCtttttt                                                                                             >  1:5206304/1‑99 (MQ=255)
                 cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGGAAGATA.AAGGGGGTTTGCTTTTTTCt                                                                                           >  1:2442788/1‑101 (MQ=39)
                 cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGGAAGATA.AAGAGGCTTTGGTTTTTTCt                                                                                           >  2:3428964/1‑101 (MQ=38)
                    cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTc                                                                                        <  1:4923615/101‑1 (MQ=255)
                    cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTc                                                                                        <  1:2152482/101‑1 (MQ=255)
                     acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTcc                                                                                       <  2:560240/101‑1 (MQ=255)
                      cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTccc                                                                                      <  1:2331062/101‑1 (MQ=255)
                      cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGAGAAGATA.AAGGGGCTTTGCTTTTTTCTTTccc                                                                                      >  2:6756948/1‑101 (MQ=255)
                           cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAat                                                                                 <  2:4577163/101‑1 (MQ=255)
                            gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGCAAGATA.AAGGGGGTTTGCTTTTTTCTTTCCCCAAata                                                                                >  1:8902952/1‑101 (MQ=255)
                             aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAata.t                                                                              <  1:7517738/101‑1 (MQ=255)
                              ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.c                                                                            <  1:5897177/101‑1 (MQ=255)
                                aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.ccc                                                                          <  2:8034285/101‑1 (MQ=255)
                                aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAAATTTGGGGGAAGATA.AAGGGGCTTTGCTTTTATCTTTTCCCAAATA.T.ccc                                                                          >  1:5589487/1‑101 (MQ=255)
                                 gCTTTCGAAAGACAGGATTCCGCCCTGCGGCCGATCCCCCCGGGGGGGATTTTTGGGCAAAAATA.AGGGGGTTTTGTTTTTTTCTTCCCCCAAATA.C.CCCg                                                                         <  2:3903873/101‑1 (MQ=255)
                                 gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCg                                                                         <  2:5986862/101‑1 (MQ=255)
                                    ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTTCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTCCTTTTATCTTTCCCCAAATA.T.CCCgggg                                                                      <  1:8090007/101‑1 (MQ=255)
                                     tCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTGTTTTCGGTCAGTTATATGGGGTTTTACTTTT.TTTTTCCCCAAATA.A.CCCgctgg                                                                     >  1:1334620/1‑97 (MQ=255)
                                     tCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGGAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.ACCggggg                                                                     >  2:4283642/1‑101 (MQ=255)
                                      cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGt                                                                    <  2:2274518/101‑1 (MQ=255)
                                       gAAAGCCGGGAATTCGCCCTGGTGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTg                                                                   <  2:6939690/101‑1 (MQ=25)
                                       gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGGAGATA.AAAGGGGGTTGCTTTTTTCTTTTCCCCAATATT.CCCgggggg                                                                    >  2:1893881/1‑100 (MQ=255)
                                        aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATA.AAGGGGGTTTACTTTTATCTTTTCCCAAATA.T.CCCaggggcgg                                                                  >  2:3767652/1‑98 (MQ=255)
                                        aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGCAAGATA.AAGGGGCTTTGCTTTTTTCTTTTCCCCAATATT.CCCggggggc                                                                   >  2:8234110/1‑99 (MQ=37)
                                         aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAAATA.AAAGGGGTTTGCTTTTTTCTTTCCCCCAATA.T.CCCGGGGGTggg                                                                 >  1:700960/1‑99 (MQ=255)
                                            accggAATTCGGCCTGCGGCCGGTGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..gggg                                                            <  1:7748947/98‑1 (MQ=255)
                                            aCAGGGATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAAATTTGGGGCAAGATA.AAGGGGGTTTGCTTTTTTTTTTCCCCAAAAA.T.CCCGGGGGGGC..gggg                                                            >  1:5453641/1‑101 (MQ=37)
                                             cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATA.AAGGGGCTTTTGTTTTTTTTTTCCCCCAAAA.TCCCCGGGGGGGC..gggg                                                            >  1:7864484/1‑101 (MQ=255)
                                             cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGCAAGATA.AAGGGGCTTTGCTTTTTTCTTTCCCCCAAAA.T.CCCGGGGGGGC..ggggg                                                           >  2:8848162/1‑101 (MQ=37)
                                              aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGc                                                          <  2:1069331/101‑1 (MQ=255)
                                                gAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCag                                                        <  1:3138952/101‑1 (MQ=255)
                                                 aaTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGGAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGCGGGGGC..GGGGGCgga                                                       >  2:9019060/1‑101 (MQ=37)
                                                 aaTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCaga                                                       <  2:6562996/101‑1 (MQ=255)
                                                    tCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGcc                                                    <  1:9009695/101‑1 (MQ=255)
                                                     cGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGGAAGATA.AAGGGGGTTTGCTTTTATTTTTTCCCAAAAA.T.CCCGGGGGTGC..GGGGGGAGGGcgc                                                   >  2:4483840/1‑99 (MQ=25)
                                                     cGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGGGC..GGGGGCAGAGccc                                                   >  2:3558817/1‑101 (MQ=255)
                                                      ggCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCCAATA.T.CCCGGGGGGGC..GGGGGGAGAGcccc                                                  >  2:4133114/1‑101 (MQ=25)
                                                       gCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCCAATA.T.CCCGGGGGTGC..GGGGGCAGAGccccc                                                 >  2:7110040/1‑101 (MQ=255)
                                                        ccTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCCcg                                                <  1:9200146/101‑1 (MQ=255)
                                                         tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATG.AA.ACCCATCCCTTTCATCTTGCCCCAAATA.T.CCCGGGGGGCCCGGGGGGCAGCGcccccc                                                <  1:4760667/94‑2 (MQ=25)
                                                         tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATG.AA.ACCCATCCCTTTCATCTTGCCCCAAATA.T.CCCGGGGGGCCCGGGGGGCAGCGcccccc                                                <  1:66026/94‑2 (MQ=25)
                                                         cTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGGTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGGGC..GGGGGCAGAGCCCCcgc                                               >  1:643425/1‑101 (MQ=255)
                                                          tGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCCcgcc                                              <  2:1672670/101‑2 (MQ=255)
                                                          tGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCCcgcc                                              <  1:4169265/101‑2 (MQ=255)
                                                            ctttcGATGCCTCCGGCGGGGATATTTGGGGGAAGATG.AA.ACCCATCCCTTTCATCTTGCCCCAAATA.T.CCCGGGGGGCCCGGGGGGCAGCGccccccgcc                                             >  2:281986/5‑97 (MQ=21)
                                                             tttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATG.AA.ACCCATCCCTTTCATCTTGCCCCAAATA.T.CCCGGGGGGCCCGGGGGGCAGCGccccccgcca                                            <  1:1588352/98‑6 (MQ=25)
                                                                cGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGGGC..GGGGGGAGAGCCCCcgccgccccg                                        >  2:5026146/1‑94 (MQ=21)
                                                                 gATGCCTCCGGCGGGGATATTTTGGGGAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGGGC..GGGGGCAGAGCCCC..CGCCGCCACgg                                     >  1:3516511/1‑101 (MQ=18)
                                                                 gATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACgg                                     >  2:4856636/1‑101 (MQ=255)
                                                                    gCCTCCGGCGGGGATATTTGGGGGGAGATA.AAAGGGGTTTGCTTTTATCTTTTCCCCAATA.T.ACCCGGGGTGC..GGGGGCAGGGGCCCC.C.CCGCCACgggtt                                  >  2:1740689/1‑98 (MQ=16)
                                                                    gCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTc                                  >  1:5037213/1‑101 (MQ=255)
                                                                      cTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCGCGGTTCAc                                >  2:2544888/1‑101 (MQ=255)
                                                                             ggggATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                         >  1:1030055/1‑101 (MQ=255)
                                                                             ggggATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                         >  1:8737506/1‑101 (MQ=255)
                                                                              gggATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCGCC..CGCCGCCACGGTTCACGCCGCGAc                        >  1:615585/1‑101 (MQ=255)
                                                                                gATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCg                      >  1:3224416/1‑101 (MQ=255)
                                                                                gATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCg                      >  2:2062352/1‑101 (MQ=255)
                                                                                 atatTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGt                     >  1:1963402/1‑101 (MQ=255)
                                                                                 atatTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGt                     >  2:6227201/1‑101 (MQ=255)
                                                                                  tttgtGGGGGAAGGAA.AGGGGGTTTTTTTTTTTTCTCCCCCCAAATG.G.CGGGGGGGGGG..GGGGGGCG.GCCCCCCCGCCGCCACGGTTCACGCCGCGACCGt                     <  1:5827622/97‑1 (MQ=255)
                                                                                  tatTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                    >  1:1788822/1‑101 (MQ=255)
                                                                                  tatTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                    >  1:459610/1‑101 (MQ=255)
                                                                                   atTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                   >  1:7119074/1‑101 (MQ=255)
                                                                                   atTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                   >  2:3268290/1‑101 (MQ=255)
                                                                                    tttGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtctc                  >  1:222636/1‑101 (MQ=255)
                                                                                     ttGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                 >  2:1262745/1‑101 (MQ=255)
                                                                                       ggggCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg               >  2:8288355/1‑101 (MQ=255)
                                                                                       ggggCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg               >  1:4026149/1‑101 (MQ=255)
                                                                                       ggggAAAGAAA.AAGGGG.TTTTTTTTTTTCTCTCCCCAAAAA.T.GCCGGGGGGGG..GGGGGGCCGGCCCCCCCGCCGCCACGGTTCACGCCGCGACCGTCTCCg                <  2:2703014/101‑1 (MQ=255)
                                                                                        gggCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg              >  2:7782745/1‑101 (MQ=255)
                                                                                         ggCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgggg             >  1:1107596/1‑101 (MQ=255)
                                                                                           cAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc           >  1:85075/1‑101 (MQ=255)
                                                                                            aaGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg          >  1:8418280/1‑101 (MQ=255)
                                                                                              gATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGGGACCGTCTCCGGGGTCGcc        >  2:586756/1‑101 (MQ=255)
                                                                                              gATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc        >  2:3119065/1‑101 (MQ=255)
                                                                                               aTA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGTGTCGCCa       >  2:2201465/1‑101 (MQ=255)
                                                                                               aTA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa       >  2:8711388/1‑101 (MQ=255)
                                                                                               aTA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa       >  1:2907221/1‑101 (MQ=255)
                                                                                               aTA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa       >  2:5503241/1‑101 (MQ=255)
                                                                                               aTA.AAGGGGCTTTGCTTTTATCTTGCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa       >  2:4382164/1‑101 (MQ=25)
                                                                                                tA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg      >  2:5363413/1‑101 (MQ=255)
                                                                                                 gatgggggCGTTTTTTTTTTTTTTCCCCAAAAA.T.GCCGGGGGGGG..GGGGGGGCGCCCCCCCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc        <  2:1477740/97‑1 (MQ=255)
                                                                                                 a.aaGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc     >  2:6439485/1‑101 (MQ=255)
                                                                                                 a.aaGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc     >  1:8619070/1‑101 (MQ=255)
                                                                                                   aaGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg    >  2:7299866/1‑101 (MQ=255)
                                                                                                   aaGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg    >  2:668987/1‑101 (MQ=255)
                                                                                                    aGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc   >  2:7537450/1‑101 (MQ=255)
                                                                                                     |                                                                                                          
GGCTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATA.AAGGGGCTTTGCTTTTATCTTTCCCCAAATA.T.CCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCG  >  NC_014034/3439691‑3439893

Base quality scores:  ATCG  < 3 ≤  ATCG  < 14 ≤  ATCG  < 26 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG