Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,846 Δ1 bp 100% intergenic (‑149/+14) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,8460C→.100.0% 15.6 319intergenic (‑149/+14)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (309/2):  ref base (0/8):  total (309/10)
Fisher's exact test strand distribution p-value = 1.85e-14
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.19e-05

Only 100 of 443 total aligned reads displayed.

GCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGTATAGAC  >  NC_014034/3439746‑3439943
                                                                                                      |                                                                                                 
gCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccccc                                                                                                   <  1:1893941/101‑1 (MQ=255)
 ccTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCcg                                                                                                  <  1:9200146/101‑1 (MQ=255)
  cTGCGGCCGATGCCTCCGGCGGGGACATTTGGGGCAAAATAAAAGGGCTTTTCTTTTTTCTTTTCCCTAATATCCCGGGGGGGG..GGGGGGAGGGCCCCcgc                                                                                                 >  1:2854043/1‑101 (MQ=25)
   tGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCcgcc                                                                                                <  2:1672670/101‑2 (MQ=255)
   tGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCcgcc                                                                                                <  1:7007310/101‑2 (MQ=255)
     ctttcGATGCCTCCGGCGGGGATATTTGGGGGAAGATGAA.ACCCATCCCTTTCATCTTGCCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGccccccgcc                                                                                               >  2:281986/5‑97 (MQ=21)
         cGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCCGGGGGGC..GGGGGCAGAGCCCCcgccgccacg                                                                                          >  2:209123/1‑94 (MQ=21)
               cTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCGCGGTTCAc                                                                                  >  2:2544888/1‑101 (MQ=255)
                 ccGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgc                                                                                >  2:7862046/1‑101 (MQ=255)
                     cggGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcg                                                                            >  2:4682804/1‑101 (MQ=255)
                      ggggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                                                                           >  1:4895523/1‑101 (MQ=255)
                         gATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCg                                                                        >  1:2635071/1‑101 (MQ=255)
                         gATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCg                                                                        >  1:8471729/1‑101 (MQ=255)
                          atatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGt                                                                       >  1:7719459/1‑101 (MQ=255)
                          atatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGt                                                                       >  1:3279019/1‑101 (MQ=255)
                          atatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGt                                                                       >  2:6227201/1‑101 (MQ=255)
                           tatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                                                                      >  1:6769859/1‑101 (MQ=255)
                           tatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                                                                      >  1:6247997/1‑101 (MQ=255)
                            atTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                                                                     >  2:3268290/1‑101 (MQ=255)
                             gttGGGGAAAAAAAAAGGGG.TTTTTTTTTTTTTCTCCCCCAAAATCCGGGGGGGGG..GGGGGGCCCGCCCCCCCGCCGCCACGGTTCACGCCGCGACCGtct                                                                     <  1:7132452/100‑1 (MQ=255)
                              ttGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                                                                   >  2:1262745/1‑101 (MQ=255)
                               tGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCAGCGACCGTCTCCg                                                                  >  2:6917978/1‑101 (MQ=255)
                                ggggCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg                                                                 >  2:8288355/1‑101 (MQ=255)
                                ggggAAAGAAAAAGGGG.TTTTTTTTTTTCTCTCCCCAAAAATGCCGGGGGGGG..GGGGGGCCGGCCCCCCCGCCGCCACGGTTCACGCCGCGACCGTCTCCg                                                                  <  2:2703014/101‑1 (MQ=255)
                                   gCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGt                                                              >  1:2316268/1‑101 (MQ=255)
                                    cAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                                                             >  2:7140683/1‑101 (MQ=255)
                                      aGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc                                                           >  2:3338342/1‑101 (MQ=255)
                                       gATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGGGACCGTCTCCGGGGTCGcc                                                          >  2:586756/1‑101 (MQ=255)
                                       gATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc                                                          >  1:9031475/1‑101 (MQ=255)
                                        aTAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                                         >  1:5075505/1‑101 (MQ=255)
                                        aTAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                                         >  2:5503241/1‑101 (MQ=255)
                                        aTAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                                         >  2:8711388/1‑101 (MQ=255)
                                        aTAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                                         >  2:3803922/1‑101 (MQ=255)
                                        aTAAAGGGGCTTTGCTTTTATCTTGCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                                         >  2:4382164/1‑101 (MQ=25)
                                         tAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcccg                                                        >  2:2257509/1‑99 (MQ=255)
                                         tAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                                                        >  2:5363413/1‑101 (MQ=255)
                                         tAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                                                        >  2:5951553/1‑101 (MQ=255)
                                          gagggggTTTTTCTTTTTACCTT.CCCACAAATGCCGGGGGGGG..GGGGGGGCGGCCCCCCCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                                                        <  2:3645747/98‑1 (MQ=255)
                                          aaaGGGTTTTTTTTTTTTTCTCTCCCCAAATATGCCGGGGGGGG..GGGGGGCCCGCCCCCCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                                         <  2:4052082/101‑1 (MQ=255)
                                          aaaGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                                                       >  2:6439485/1‑101 (MQ=255)
                                          aaaGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                                                       >  2:7315059/1‑101 (MQ=255)
                                          aaaGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                                                       >  2:7629704/1‑101 (MQ=255)
                                          aaaGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                                                       >  2:4499434/1‑101 (MQ=255)
                                          aaaGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                                                       >  2:8204292/1‑101 (MQ=255)
                                           aaGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg                                                      >  2:4139268/1‑101 (MQ=255)
                                           aaGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg                                                      >  2:7956935/1‑101 (MQ=255)
                                            aGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgggc                                                     >  2:878762/1‑101 (MQ=255)
                                            aGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc                                                     >  1:2106474/1‑101 (MQ=255)
                                            aGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc                                                     >  2:2340196/1‑101 (MQ=255)
                                             ggggCTTTGCTTTTATCTTTCCCCAAATATCGGGGGGGTGC..GGGAGCAGCGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGcgcg                                                    <  1:1310388/101‑1 (MQ=255)
                                              gggCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga                                                   >  1:52683/1‑101 (MQ=255)
                                              gggCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga                                                   >  1:4563049/1‑101 (MQ=255)
                                               ggCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgac                                                  >  1:5843036/1‑101 (MQ=255)
                                                 ctttgctttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacga                                                >  2:7036667/1‑101 (MQ=255)
                                                   ttgctttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCTCGACCGTCTCCGGGGTCGCCAGCGCGACGata                                              >  2:6750772/1‑101 (MQ=255)
                                                    tgctttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat                                             >  1:4289663/1‑101 (MQ=255)
                                                      ctttTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATcc                                           >  1:4759911/1‑101 (MQ=255)
                                                       ttttATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATccc                                          >  2:8966843/1‑100 (MQ=255)
                                                       ttttATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCca                                          >  1:875261/1‑101 (MQ=255)
                                                       ttttATCTTTCCCCAAATATCCCGGGGGCGC..GGCGGCAGAGCCCCC.C.ACGCCAGGGCTCACGCCGGCCCAGCCTCCCGCGCCGCCAGTGCGACGGTCTCca                                          >  2:3080650/1‑101 (MQ=9)
                                                        tttATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcat                                         >  2:2197063/1‑101 (MQ=255)
                                                         ttATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatc                                        >  2:5736856/1‑101 (MQ=255)
                                                           aTCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatcat                                      >  1:175946/1‑101 (MQ=255)
                                                             cTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGa                                    >  2:2469681/1‑101 (MQ=255)
                                                             cTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGa                                    >  1:5647443/1‑101 (MQ=255)
                                                             cTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGa                                    >  1:1010355/1‑101 (MQ=255)
                                                                  cccAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCgg                               >  2:7430257/1‑101 (MQ=255)
                                                                    cAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGtt                             >  2:4795168/1‑101 (MQ=255)
                                                                    cAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGtt                             >  1:8097128/1‑101 (MQ=255)
                                                                    cAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGtt                             >  1:2415550/1‑101 (MQ=255)
                                                                    cAAATATCCCGGAGGTGC..GGGGGCAGAGGCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGtt                             >  1:6514060/1‑101 (MQ=255)
                                                                     aaaTATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTc                            >  2:9170946/1‑101 (MQ=255)
                                                                     aaaTATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTc                            >  2:1972340/1‑101 (MQ=255)
                                                                     aaaTATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTc                            >  1:8340846/1‑101 (MQ=255)
                                                                       atatCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAg                          >  2:5599507/1‑101 (MQ=255)
                                                                       atatCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAg                          >  1:3523952/1‑101 (MQ=255)
                                                                       agagggcGGGGGGGG..GGGGGCCGCGCCCCCCCACCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTc                            <  2:1030037/95‑1 (MQ=255)
                                                                        tatCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGc                         >  1:4154344/1‑101 (MQ=255)
                                                                        tatCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGc                         >  2:9072042/1‑101 (MQ=255)
                                                                        tatCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGc                         >  2:1513947/1‑101 (MQ=255)
                                                                         atCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCt                        >  1:1181940/1‑101 (MQ=255)
                                                                         atCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCt                        >  2:8545604/1‑101 (MQ=255)
                                                                          tCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTc                       >  1:7935671/1‑101 (MQ=255)
                                                                          tCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTc                       >  1:8886052/1‑101 (MQ=255)
                                                                             cGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGaa                    >  1:7388196/1‑101 (MQ=255)
                                                                              gggggTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGaaa                   >  2:6617901/1‑101 (MQ=255)
                                                                                gggTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATc                 >  2:8170965/1‑101 (MQ=255)
                                                                                 ggTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGGCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATcc                >  1:2206747/1‑101 (MQ=255)
                                                                                        gggggCGGCGCCCCCCCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTc             <  2:3516404/101‑1 (MQ=255)
                                                                                        gggggCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCgg           >  2:2976922/1‑101 (MQ=255)
                                                                                        gggggCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCgg           >  1:3911728/1‑101 (MQ=255)
                                                                                           ggCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGt        >  1:3011276/1‑101 (MQ=255)
                                                                                           ggCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGt        >  1:7634444/1‑101 (MQ=255)
                                                                                               ccccccccCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGtat      <  1:5552940/98‑1 (MQ=255)
                                                                                               ccccccccCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGtat      <  1:8545989/98‑1 (MQ=255)
                                                                                               cagagcccCCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGtat      >  2:4989933/6‑101 (MQ=255)
                                                                                                agagcccCCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGtata     >  2:1175779/5‑101 (MQ=255)
                                                                                                   ccccCCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGTATAGAc  <  1:6705581/101‑1 (MQ=255)
                                                                                                   ccccCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGTATAGAc  <  1:4447936/101‑1 (MQ=255)
                                                                                                   ccccCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGTATAGAc  <  2:1866365/101‑1 (MQ=255)
                                                                                                      |                                                                                                 
GCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCTTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGTATAGAC  >  NC_014034/3439746‑3439943

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG