Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,440,947 Δ1 bp 0.9% coding (887/1974 nt) mutB ← methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,440,9470C→. 0.9% 3.2 ‑1.1 ‑0.1424coding (887/1974 nt)mutBmethylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (193/226):  total (197/227)
Fisher's exact test strand distribution p-value = 3.41e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.16e-01

Only 100 of 528 total aligned reads displayed.

GGTTTCATAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCTGTTCAC  >  NC_014034/3440847‑3441047
                                                                                                    |                                                                                                    
  tttCATAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCAccgcccgc                                                                                                    >  1:2905939/1‑101 (MQ=255)
   ttCATAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCcgcg                                                                                                   >  2:7648292/1‑101 (MQ=255)
    tCATAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCcgcgc                                                                                                  >  1:6114058/1‑101 (MQ=255)
    tCATAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCcgcgc                                                                                                  <  1:5143180/101‑1 (MQ=255)
      aTAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTGGTTCCAGGCCGGAAGCTGCACCGCCcgcgcgc                                                                                                >  1:4560741/1‑101 (MQ=255)
      aTAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCcgcgcgc                                                                                                >  1:1220836/1‑101 (MQ=255)
      aTAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCcgcgcgc                                                                                                >  1:6212098/1‑101 (MQ=255)
      aTAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCcgcgcgc                                                                                                >  1:3457755/1‑101 (MQ=255)
       tAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCcgcgcgcg                                                                                               >  1:8311297/1‑101 (MQ=255)
         ggCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCcgcgcgcgcg                                                                                             >  1:5238/1‑101 (MQ=255)
         ggCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCcgcgcgcgcg                                                                                             >  2:4583156/1‑101 (MQ=255)
          gCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCgcgcgcgcgc                                                                                            <  2:8527662/101‑1 (MQ=255)
            cATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCAt                                                                                          <  1:1657737/101‑1 (MQ=255)
            cATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCAt                                                                                          >  2:3048648/1‑101 (MQ=255)
            cATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCAt                                                                                          <  2:1426611/101‑1 (MQ=255)
                 tCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCAttcttc                                                                                     <  2:5966299/101‑1 (MQ=255)
                 tCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCAttcttc                                                                                     >  2:4694103/1‑101 (MQ=255)
                   tgctgcATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCACTCTTCGa                                                                                   >  2:1608952/1‑101 (MQ=255)
                    gctgcATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGAc                                                                                  >  2:6673411/1‑101 (MQ=255)
                    gctgcATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGAc                                                                                  >  1:5535170/1‑101 (MQ=255)
                     ctgcATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACa                                                                                 >  2:3455625/1‑101 (MQ=255)
                     ctgcATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACa                                                                                 >  1:4887101/1‑101 (MQ=255)
                      tgcATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAg                                                                                <  1:2450652/101‑1 (MQ=255)
                         aTGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCgc                                                                             >  2:8451469/1‑100 (MQ=255)
                         aTGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGt                                                                             >  2:7544549/1‑101 (MQ=255)
                          tGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTc                                                                            <  2:2220561/101‑1 (MQ=255)
                            cgcAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCAc                                                                          <  2:7305502/101‑1 (MQ=255)
                               aGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGc                                                                       >  1:5331750/1‑101 (MQ=255)
                                gCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGcc                                                                      <  1:2774468/101‑1 (MQ=255)
                                gCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGcc                                                                      <  1:3604715/101‑1 (MQ=255)
                                  gACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAg                                                                    >  1:6676828/1‑101 (MQ=255)
                                  gACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAg                                                                    >  1:3781788/1‑101 (MQ=255)
                                  gACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCCCCAg                                                                    <  2:2905841/101‑1 (MQ=255)
                                   aCCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGc                                                                   <  1:6008123/101‑1 (MQ=255)
                                   aCCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGc                                                                   <  1:4615225/101‑1 (MQ=255)
                                      aTTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCAtc                                                                <  1:4742420/101‑1 (MQ=255)
                                      aTTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCAtc                                                                >  2:1090989/1‑101 (MQ=255)
                                        tGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCAtctc                                                              <  1:8512680/101‑1 (MQ=255)
                                        tGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCAtctc                                                              >  2:393709/1‑101 (MQ=255)
                                         gCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCg                                                             >  1:3158296/1‑101 (MQ=255)
                                         gCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCg                                                             >  1:1385355/1‑101 (MQ=255)
                                           tGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCCTCTCGAt                                                           >  2:1268194/1‑101 (MQ=255)
                                           tGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGAt                                                           >  1:7270893/1‑101 (MQ=255)
                                            gATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATg                                                          <  2:2372420/101‑1 (MQ=255)
                                             aTCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATga                                                         <  1:5665918/101‑1 (MQ=255)
                                              acccAGGGCCGCGGCAGGCCCAGCGCGTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATgag                                                        <  2:8886858/100‑1 (MQ=255)
                                               cccAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATgaga                                                       <  1:3555583/101‑1 (MQ=255)
                                               cccAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATgaga                                                       <  2:5590858/101‑1 (MQ=255)
                                                 cAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAAt                                                     >  1:7834349/1‑101 (MQ=255)
                                                  aGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATc                                                    <  1:189741/101‑1 (MQ=255)
                                                  aGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATc                                                    <  1:1487957/101‑1 (MQ=255)
                                                  aGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATc                                                    >  2:370614/1‑101 (MQ=255)
                                                  aGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATc                                                    <  1:4328868/101‑1 (MQ=255)
                                                   gggCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATcc                                                   <  1:2328367/101‑1 (MQ=255)
                                                   gggCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATcc                                                   <  2:4258014/101‑1 (MQ=255)
                                                   gggCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATcc                                                   >  2:4082712/1‑101 (MQ=255)
                                                      ccGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGAt                                                <  1:6942789/101‑1 (MQ=255)
                                                      ccGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAACTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGAt                                                <  2:2690050/101‑1 (MQ=255)
                                                       cgcgGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATa                                               >  2:7946937/1‑101 (MQ=255)
                                                       cgcgGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATa                                               <  1:7682242/101‑1 (MQ=255)
                                                       cccgGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATa                                               <  2:6526244/99‑1 (MQ=255)
                                                          ggCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGAc                                            <  1:932297/101‑1 (MQ=255)
                                                            cAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgt                                          >  1:2158803/1‑101 (MQ=255)
                                                            cAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgt                                          >  2:1759298/1‑101 (MQ=255)
                                                             aGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGCTAGACgtt                                         >  1:4256817/1‑101 (MQ=255)
                                                             aGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgtt                                         <  1:4053827/101‑1 (MQ=255)
                                                               gCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgttgt                                       >  2:7166071/1‑101 (MQ=255)
                                                                cccAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgttgtt                                      <  2:579140/101‑1 (MQ=255)
                                                                cccAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgttgtt                                      >  2:6169362/1‑101 (MQ=255)
                                                                cccAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgttgtt                                      <  2:6235563/101‑1 (MQ=255)
                                                                cccAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgttgtt                                      <  2:417637/101‑1 (MQ=255)
                                                                cccAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgttgtt                                      <  1:1065444/101‑1 (MQ=255)
                                                                cccAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgttgtt                                      <  1:4413601/101‑1 (MQ=255)
                                                                 ccAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGTGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGttt                                     <  2:4362694/101‑1 (MQ=255)
                                                                   aGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTc                                   <  2:8236025/101‑1 (MQ=255)
                                                                   aGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTc                                   <  2:5269011/101‑1 (MQ=255)
                                                                   aGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTc                                   >  2:6921734/1‑101 (MQ=255)
                                                                   aGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTc                                   >  1:7427693/1‑101 (MQ=255)
                                                                     cgcCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCg                                 >  1:6829977/1‑101 (MQ=255)
                                                                     cgcCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCg                                 >  1:8000294/1‑101 (MQ=255)
                                                                        cTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGgct                              <  2:2753305/101‑1 (MQ=255)
                                                                          cGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGgctgc                            >  2:3566990/1‑101 (MQ=255)
                                                                           gTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGgctgct                           >  1:3902677/1‑101 (MQ=255)
                                                                            ttCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGgctgctg                          >  1:7321305/1‑101 (MQ=255)
                                                                             tCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGgctgctgc                         >  2:5152288/1‑101 (MQ=255)
                                                                                   ccGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTc                   >  2:1023479/1‑101 (MQ=255)
                                                                                   ccGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTc                   <  2:903353/101‑1 (MQ=255)
                                                                                     ggAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCGCCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAg                 >  2:4446326/1‑101 (MQ=255)
                                                                                     ggAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAg                 <  1:7102784/101‑1 (MQ=255)
                                                                                       aaGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGAc               <  1:577130/101‑1 (MQ=255)
                                                                                           tGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAg           >  2:6751827/1‑101 (MQ=255)
                                                                                            gCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAgg          >  1:5814447/1‑101 (MQ=255)
                                                                                            gCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAgg          >  2:2017552/1‑101 (MQ=255)
                                                                                             cACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGc         >  2:4871054/1‑101 (MQ=255)
                                                                                                cgcccgcGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCTGt      <  2:7779554/101‑1 (MQ=255)
                                                                                                cgcccgcGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCTGt      <  1:4967390/101‑1 (MQ=255)
                                                                                                 gcccgcGCGGGCGCATTCTTCGGCAGCGTGAGCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCTGtt     <  2:9183980/101‑1 (MQ=255)
                                                                                                 gcccgcGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCTGtt     <  1:8744695/101‑1 (MQ=255)
                                                                                                 gcccgcGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCTGtt     <  1:891387/101‑1 (MQ=255)
                                                                                                   ccgcGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCTGTTCa   <  2:1220314/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GGTTTCATAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCTGTTCAC  >  NC_014034/3440847‑3441047

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG