Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,440,948 Δ1 bp 1.0% coding (886/1974 nt) mutB ← methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,440,9480G→. 1.0% 3.3 ‑1.1 ‑0.3420coding (886/1974 nt)mutBmethylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (192/224):  total (195/225)
Fisher's exact test strand distribution p-value = 3.41e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.00e-01

Only 100 of 523 total aligned reads displayed.

GTTTCATAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCTGTTCACC  >  NC_014034/3440848‑3441048
                                                                                                    |                                                                                                    
gTTTCATAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCAccgcccg                                                                                                      >  2:4591836/1‑101 (MQ=255)
gTTTCATAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCAccgcccg                                                                                                      >  1:4631156/1‑101 (MQ=255)
  ttCATAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCcgcg                                                                                                    >  1:1492813/1‑101 (MQ=255)
  ttCATAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCcgcg                                                                                                    >  2:7648292/1‑101 (MQ=255)
     aTAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCcgcgcgc                                                                                                 >  1:1220836/1‑101 (MQ=255)
     aTAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCcgcgcgc                                                                                                 >  1:3457755/1‑101 (MQ=255)
      tAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCcgcgcgcg                                                                                                >  1:3560573/1‑101 (MQ=255)
      tAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCcgcgcgcg                                                                                                >  2:593962/1‑101 (MQ=255)
       aGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCcgcgcgcgc                                                                                               <  2:7039114/101‑1 (MQ=255)
           cATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCAt                                                                                           >  2:7199331/1‑101 (MQ=255)
           cATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCAt                                                                                           <  1:1657737/101‑1 (MQ=255)
            atactctcgtgcATGCGCAGCTACCATAGATGATCCCAGGGCCGCGGAAGACCCAGCGCCTCGTTCCAGGCCGGAAACTGCACCGCCCGCGCGCGCGCAtt                                                                                          <  2:1253214/92‑1 (MQ=255)
            aTGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCAtt                                                                                          >  1:7951902/1‑101 (MQ=255)
             tGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCAttc                                                                                         >  2:3723160/1‑101 (MQ=255)
              gATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCAttct                                                                                        <  1:8471725/101‑1 (MQ=255)
              gATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCAntct                                                                                        >  1:6025591/1‑101 (MQ=255)
               aTCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCAttctt                                                                                       <  1:4490331/101‑1 (MQ=255)
               aTCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCAttctt                                                                                       >  1:492511/1‑101 (MQ=255)
                tCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCAttcttc                                                                                      >  2:4694103/1‑101 (MQ=255)
                tCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCAttcttc                                                                                      <  2:2538408/101‑1 (MQ=255)
                 ctgctgCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCg                                                                                     >  1:7305806/1‑101 (MQ=255)
                  tgctgcATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGa                                                                                    >  2:5080877/1‑101 (MQ=255)
                  tgctgcATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGa                                                                                    >  2:2994693/1‑101 (MQ=255)
                   gctgcATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGAc                                                                                   >  2:6673411/1‑101 (MQ=255)
                   gctgcATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGAc                                                                                   <  1:5259885/101‑1 (MQ=255)
                     tgcATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAg                                                                                 <  1:2450652/101‑1 (MQ=255)
                        aTGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCgc                                                                              >  2:8451469/1‑100 (MQ=255)
                        aTGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGt                                                                              <  1:8278305/101‑1 (MQ=255)
                        aTGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGt                                                                              >  2:7544549/1‑101 (MQ=255)
                           cgcAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCAc                                                                           <  1:4762086/101‑1 (MQ=255)
                            gcAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCAcc                                                                          <  1:5356027/101‑1 (MQ=255)
                                cGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCa                                                                      >  1:5676838/1‑101 (MQ=255)
                                    cATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCac                                                                  >  2:5250261/1‑100 (MQ=255)
                                    cATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCAt                                                                  <  1:580188/101‑1 (MQ=255)
                                    cATTGCGGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCAt                                                                  <  1:1439288/101‑1 (MQ=255)
                                     aTTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCAtc                                                                 <  1:1985352/101‑1 (MQ=255)
                                     aTTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCAtc                                                                 <  2:7831637/101‑1 (MQ=255)
                                       tGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCAtctc                                                               >  2:393709/1‑101 (MQ=255)
                                        gCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCg                                                              >  1:3158296/1‑101 (MQ=255)
                                           gATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCGCCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATg                                                           <  1:9040948/101‑1 (MQ=255)
                                           gATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATg                                                           <  1:6876880/101‑1 (MQ=255)
                                           gATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATg                                                           >  1:8642683/1‑101 (MQ=255)
                                           gATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATg                                                           >  1:4340000/1‑101 (MQ=255)
                                            aTCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATga                                                          <  2:3194468/101‑1 (MQ=255)
                                             acccAGGGCCGCGGCAGGCCCAGCGCGTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATgag                                                         <  2:8886858/100‑1 (MQ=255)
                                              cccAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATgaga                                                        >  1:7407495/1‑101 (MQ=255)
                                               ccAGGACCGCGGCAGCCCCAGCGCCCCCTTCCAGGCAGGAAGCTGCAACGCCCGCGCGCGCACATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGaa                                                       <  2:7307299/101‑1 (MQ=255)
                                                  gggCCGGGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCGCCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATcc                                                    <  2:8621490/101‑1 (MQ=255)
                                                  gggCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATcc                                                    >  2:4082712/1‑101 (MQ=255)
                                                  gggCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATcc                                                    >  1:2385716/1‑101 (MQ=255)
                                                  gggCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATcc                                                    >  1:98895/1‑101 (MQ=255)
                                                    gCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGa                                                  >  1:7779887/1‑101 (MQ=255)
                                                      cgcgGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATa                                                >  2:7946937/1‑101 (MQ=255)
                                                      cccgGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATa                                                <  2:6526244/99‑1 (MQ=255)
                                                       gcgGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAg                                               >  2:2780210/1‑101 (MQ=255)
                                                         ggCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGAc                                             >  1:6715761/1‑101 (MQ=255)
                                                         ggCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGAc                                             >  1:5577338/1‑101 (MQ=255)
                                                          gcaagccaggcgcCTCCTTCCAGGCCGGAAACTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACg                                            <  2:1656473/92‑1 (MQ=255)
                                                            aGGCCCAGCGCCTCGTTCCATGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgtt                                          <  1:7199635/101‑1 (MQ=255)
                                                            aGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgtt                                          <  2:2158741/101‑1 (MQ=255)
                                                            aGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgtt                                          <  1:4053827/101‑1 (MQ=255)
                                                               cccAGCGCCTCGTTCCAGGCCGGAAGCTGCACGGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgttgtt                                       <  1:4560791/101‑1 (MQ=255)
                                                               cccAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgttgtt                                       <  2:6235563/101‑1 (MQ=255)
                                                               cccAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgttgtt                                       >  2:6143957/1‑101 (MQ=255)
                                                               cccAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACgttgtt                                       <  1:1065444/101‑1 (MQ=255)
                                                                ccAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGTGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGttt                                      <  2:4362694/101‑1 (MQ=255)
                                                                ccAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGttt                                      <  1:5168275/101‑1 (MQ=255)
                                                                 cAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGtttt                                     >  2:3604110/1‑101 (MQ=255)
                                                                  aGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTc                                    <  2:8236025/101‑1 (MQ=255)
                                                                  aGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTc                                    <  1:2234586/101‑1 (MQ=255)
                                                                   gcgcCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTcc                                   <  2:5780002/101‑1 (MQ=255)
                                                                   gccccTCCTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTcc                                   <  2:4291546/98‑1 (MQ=255)
                                                                     gcCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCgg                                 >  1:6344574/1‑101 (MQ=255)
                                                                     gcCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGACAGCATATCGATGAGAATCCGATAGACGTTGTTTTCAgg                                 >  1:3645509/1‑101 (MQ=255)
                                                                      ccTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGgc                                >  1:4302271/1‑101 (MQ=255)
                                                                       cTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGgct                               >  2:1992966/1‑101 (MQ=255)
                                                                         cGTTCCAGGCCGGAAGCTGCACCGCC..CGCGCGCGCATACTTTGACACCGTCACCGCGAGCATCTCGATGTGAGTCCGGTAGACGTTGTTTTTCGgctgtgc                           >  2:1190631/1‑98 (MQ=255)
                                                                          gTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGgctgct                            <  1:8908808/101‑1 (MQ=255)
                                                                          gTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGgcggct                            >  1:2933430/1‑101 (MQ=255)
                                                                            tCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGgctgctgc                          >  1:7552089/1‑101 (MQ=255)
                                                                              cAGGTCGGAAGCTGCGCCGTCCGCGCGCGCGCATTCTTCGACAGCGTCATCCCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTc                        <  2:5534963/101‑1 (MQ=255)
                                                                                  ccGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTc                    <  2:903353/101‑1 (MQ=255)
                                                                                    ggAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCGCCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAg                  >  2:4446326/1‑101 (MQ=255)
                                                                                     gAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGa                 >  1:3788002/1‑101 (MQ=255)
                                                                                       aGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGAcc               >  1:2780101/1‑101 (MQ=255)
                                                                                       aGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGAcc               >  2:1066839/1‑101 (MQ=255)
                                                                                         cTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCaa             >  2:1570535/1‑101 (MQ=255)
                                                                                         cTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCaa             >  2:2727840/1‑101 (MQ=255)
                                                                                          tGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAg            >  2:6751827/1‑101 (MQ=255)
                                                                                           gCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAgg           <  2:3457651/101‑1 (MQ=255)
                                                                                            cACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGc          >  2:4871054/1‑101 (MQ=255)
                                                                                             aCCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCt         >  1:7064159/1‑101 (MQ=255)
                                                                                             aCCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCt         >  2:1379252/1‑101 (MQ=255)
                                                                                              ccgcccgcGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCACCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGAACAAGGCTg        >  2:4178732/1‑101 (MQ=255)
                                                                                               cgcccgcGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCTGt       <  1:4967390/101‑1 (MQ=255)
                                                                                               cgcccgcGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCTGt       <  1:6144194/101‑1 (MQ=255)
                                                                                                gcccgcGCGGGCGCATTCTTCGGCAGCGTGAGCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCTGtt      <  2:9183980/101‑1 (MQ=255)
                                                                                                 cccgcGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCTGTTc     <  2:2285060/101‑1 (MQ=255)
                                                                                                   cgcgcgcgcgCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCTGTTCAc   <  1:5894353/101‑1 (MQ=255)
                                                                                                    gcgcgcgcgcATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGACTGCTGCTCGGTCAGACCAAGGCTGTTCAcc  >  1:3920066/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GTTTCATAGGCCATGATCTGCTGCATGCGCAGCGACCATTGCTGATCCCAGGGCCGCGGCAGGCCCAGCGCCTCGTTCCAGGCCGGAAGCTGCACCGCCCGCGCGCGCGCATTCTTCGACAGCGTCACCGCCAGCATCTCGATGAGAATCCGATAGACGTTGTTTTCCGGCTGCTGCTCGGTCAGACCAAGGCTGTTCACC  >  NC_014034/3440848‑3441048

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG