Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,485,050 Δ1 bp 1.2% coding (13/654 nt) rnfG → electron transport complex protein RnfG

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,485,0500C→. 1.2% 3.5 ‑0.5 ‑0.0326coding (13/654 nt)rnfGelectron transport complex protein RnfG
Reads supporting (aligned to +/- strand):  new base (1/3):  ref base (161/161):  total (162/164)
Fisher's exact test strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.89e-01

Only 100 of 403 total aligned reads displayed.

TCGAAACCTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGTGCTGCTGGC  >  NC_014034/3484950‑3485150
                                                                                                     |                                                                                                     
tCGAAACCTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.c                                                                                                       >  1:5383384/1‑101 (MQ=255)
 cGAAACCTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.cc                                                                                                      >  2:4303340/1‑101 (MQ=255)
 cGAAACCTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.cc                                                                                                      >  1:1709331/1‑101 (MQ=255)
  gAAACCTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.ccc                                                                                                     <  1:101843/101‑1 (MQ=255)
   aaaCCTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGGAGGGAGCGCAGAAAGGACCGACACA.ccac                                                                                                    >  2:2789860/1‑99 (MQ=255)
   aaaCCTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.cccc                                                                                                    >  1:3638826/1‑101 (MQ=255)
    acccTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.ccccc                                                                                                   <  1:1347286/99‑1 (MQ=255)
    aaCCTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.ccccc                                                                                                   <  2:3071299/101‑1 (MQ=255)
     aCCTACACCCGGCCGCGGATCTTCGGACGGTCCCGCCCCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.cccccc                                                                                                  <  1:1851470/101‑1 (MQ=255)
      ccTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.ccccccc                                                                                                 >  2:7930132/1‑101 (MQ=255)
       cTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.cccccccc                                                                                                <  1:5091622/101‑1 (MQ=255)
       cTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.cccccccc                                                                                                >  1:9196483/1‑101 (MQ=255)
        tACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.ccccccccc                                                                                               >  2:3828638/1‑101 (MQ=255)
        tACACCCGGCCGGGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.ccccccccc                                                                                               <  2:6430879/101‑1 (MQ=255)
         ccacccGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCg                                                                                              <  1:5271689/97‑1 (MQ=255)
         ccATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCg                                                                                              <  2:7429621/100‑1 (MQ=255)
         aCATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCg                                                                                              <  1:4176513/101‑1 (MQ=255)
         aCATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCg                                                                                              <  1:394834/101‑1 (MQ=255)
          cacccGGCCGCGGATCTTCGGACGGCCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGa                                                                                             <  1:5532314/98‑1 (MQ=255)
          cATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.cccccccccca                                                                                             >  2:4632630/1‑99 (MQ=255)
              cGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGaaaaa                                                                                         <  1:6082907/101‑1 (MQ=255)
               ggCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAAc                                                                                        <  1:6287515/101‑1 (MQ=255)
               ggCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAAc                                                                                        <  1:1396703/101‑1 (MQ=255)
                gCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAAcc                                                                                       >  2:8336648/1‑101 (MQ=255)
                   gcgGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGaa                                                                                    >  2:4916572/1‑101 (MQ=255)
                     ggATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAAc                                                                                  >  1:8655069/1‑101 (MQ=255)
                       aTCTTCGGACGGCCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTg                                                                                <  2:1009562/101‑1 (MQ=255)
                         cTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGcc                                                                              <  1:7694092/101‑1 (MQ=255)
                              gACGGTCCCGCCCCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGt                                                                         <  2:5303584/101‑1 (MQ=255)
                               aCGGTCCCCCACCGGAAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGtt                                                                        <  2:3928010/101‑1 (MQ=255)
                                cGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTc                                                                       <  2:2537050/101‑1 (MQ=255)
                                cGGACCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCACCCCGAAAACACGAAAGTACCCTGGttt                                                                       >  2:6218331/1‑100 (MQ=255)
                                 ggccccGCCCCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACGCCCCCCCCCCGAAAAACCGAAACTGCCCTGGTTc                                                                       <  2:7012087/98‑1 (MQ=255)
                                 ggTCCCGCCCCGGCAAGCCGCTTGGCGCCGCCAACCCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.a                                                                     <  2:1687154/101‑1 (MQ=255)
                                 ggTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.a                                                                     <  2:243235/101‑1 (MQ=255)
                                 ggTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.a                                                                     >  1:4877344/1‑101 (MQ=255)
                                 ggTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.a                                                                     <  1:2535168/101‑1 (MQ=255)
                                 ggTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCACCCCCCGAAAAACCGAAACTGCCCTGGTTC.a                                                                     >  1:8164379/1‑101 (MQ=255)
                                   tCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAg                                                                   <  1:908325/101‑1 (MQ=255)
                                   ccccGCACCGGAAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGCCCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAg                                                                   <  2:3358075/100‑1 (MQ=255)
                                      cGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGgcc                                                                >  2:513963/1‑101 (MQ=255)
                                      cGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTCCCCTGGTTC.AAGgcc                                                                <  1:7256794/101‑1 (MQ=255)
                                       gcgccGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGCCCGACACC.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGgcct                                                               <  1:2081987/98‑1 (MQ=255)
                                         cccGGCAAGCCGCTTGGCGCCGCCAATCCCGCGAAGGGAGCGCAGAAATGCCCGACACG.CCCCCCCCCGAAAAGCCGAAACTGCCCTGGTTC.AAGgcctcg                                                             <  1:390492/100‑1 (MQ=255)
                                         aCCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGgcctcg                                                             >  2:7175817/1‑101 (MQ=255)
                                            ggCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGgcctcgcct                                                          <  1:8443162/101‑1 (MQ=255)
                                             gCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGgcctcgcctc                                                         >  1:6868505/1‑101 (MQ=255)
                                               aaGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTg                                                       >  2:4496477/1‑101 (MQ=255)
                                               aaGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTg                                                       <  2:3530348/101‑1 (MQ=255)
                                               aaGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTg                                                       >  2:2254666/1‑101 (MQ=255)
                                                 gCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCAGgc                                                     <  1:7418330/101‑1 (MQ=255)
                                                     cTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCAc                                                 <  1:3828747/101‑1 (MQ=255)
                                                     cTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACCGCCCCGGGTCAAAGgcctccccccggggccc                                                  >  1:8066062/1‑92 (MQ=255)
                                                      ttGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACGCCCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCAc                                                 <  2:8458262/101‑1 (MQ=255)
                                                      ttGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACg                                                <  1:4686490/101‑1 (MQ=255)
                                                           gccgccAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATc                                           <  1:2884719/101‑1 (MQ=255)
                                                           gccgccAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATc                                           <  1:8264288/101‑1 (MQ=255)
                                                             cgccAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCAt                                         <  1:4303461/101‑1 (MQ=255)
                                                             cgccAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCAt                                         <  1:643222/101‑1 (MQ=255)
                                                              gccAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATg                                        >  1:7926987/1‑101 (MQ=255)
                                                                 aaaCCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTg                                     >  1:3518276/1‑101 (MQ=255)
                                                                  aaCCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTgg                                    <  2:1491999/101‑1 (MQ=255)
                                                                  aaCCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTCGCGCACGGGATCATGCTgg                                    >  2:906399/1‑101 (MQ=255)
                                                                   aCCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGc                                   <  2:5685366/101‑1 (MQ=255)
                                                                   aCCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGc                                   <  2:3638718/101‑1 (MQ=255)
                                                                    cccGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCg                                  >  1:7543721/1‑101 (MQ=255)
                                                                     ccGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCgg                                 >  1:4551301/1‑100 (MQ=255)
                                                                        cgAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGt                              <  1:7874603/101‑1 (MQ=255)
                                                                         gAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGtt                             >  2:2618210/1‑101 (MQ=255)
                                                                          aaGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGttt                            <  2:7381450/101‑1 (MQ=255)
                                                                          aaGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGttt                            <  2:7603038/101‑1 (MQ=255)
                                                                          aaGGGAGCGCAGAAATGACCGAAACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCCCTGGCGCACGGGACCATGCCGGGGacggct                            >  2:2952794/1‑96 (MQ=255)
                                                                            gggAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGCTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTgc                          >  2:5228110/1‑101 (MQ=255)
                                                                              gAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTgcgc                        >  1:581795/1‑101 (MQ=255)
                                                                               aGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCt                       <  2:8654683/101‑1 (MQ=255)
                                                                                   cAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTc                   <  1:6200273/101‑1 (MQ=255)
                                                                                   cAGAAATGACCCACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTc                   <  1:8135192/101‑1 (MQ=255)
                                                                                     gAAATGCCCGCCACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCAc                 <  2:3982362/101‑1 (MQ=255)
                                                                                     gAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCAc                 >  1:1524384/1‑101 (MQ=255)
                                                                                     gAAAGGACCGACCCC.CCCCCCCCCGAAAAACGGAGACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCAc                 <  1:2476753/101‑1 (MQ=255)
                                                                                      aaaTGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCAcc                >  2:2829496/1‑101 (MQ=255)
                                                                                      aaaTGACCGACACG.CCCCCCCCCGAAAAACCGAAACGGCCCTGGGTC.AAGGCCTCGCCTCCTGCGCACGGAATCATTCTGGCGATGTTTGCGCTggggacc                >  2:1789660/1‑96 (MQ=255)
                                                                                       aaTGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACcg               <  2:5527054/101‑1 (MQ=255)
                                                                                       aaTGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACcg               >  2:1553561/1‑101 (MQ=255)
                                                                                        aTGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACcgc              <  1:5228279/101‑1 (MQ=255)
                                                                                        aTGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACcgc              >  2:7877194/1‑101 (MQ=255)
                                                                                         tggccGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACcgcg             <  1:3995155/98‑1 (MQ=255)
                                                                                            ccGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGtg          >  1:340195/1‑101 (MQ=255)
                                                                                             cGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGtgc         >  1:8967919/1‑101 (MQ=255)
                                                                                             cGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGtgc         <  1:8832946/101‑1 (MQ=255)
                                                                                              gACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGtgct        >  1:2711293/1‑101 (MQ=255)
                                                                                              gACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCAGGTCACCGCGGtgct        >  1:1639683/1‑101 (MQ=255)
                                                                                                cacG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGtgctgc      <  2:5070869/101‑1 (MQ=255)
                                                                                                cacG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGtgctgc      >  1:6665964/1‑101 (MQ=255)
                                                                                                 ccG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGTgctgct     <  1:2996154/100‑1 (MQ=255)
                                                                                                 acG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGTgctgct     <  2:1200391/101‑1 (MQ=255)
                                                                                                  cG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGTGctgctg    >  2:6658187/1‑101 (MQ=255)
                                                                                                   g.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGTGCTGCTgg   >  1:3199067/1‑101 (MQ=255)
                                                                                                     cccccccccGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGTGCTGCTGGc  <  1:197272/101‑1 (MQ=255)
                                                                                                     cccccccccGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGTGCTGCTGGc  >  1:1138145/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
TCGAAACCTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGACACG.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTC.AAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGTGCTGCTGGC  >  NC_014034/3484950‑3485150

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG