Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,596,943 Δ1 bp 5.3% coding (161/1467 nt) RCAP_rcc03392 → hemolysin D

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,596,9430C→. 5.3% 28.3 ‑2.8 ‑0.1228coding (161/1467 nt)RCAP_rcc03392hemolysin D
Reads supporting (aligned to +/- strand):  new base (4/8):  ref base (138/78):  total (142/86)
Fisher's exact test strand distribution p-value = 6.15e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.39e-01

Only 100 of 279 total aligned reads displayed.

CAATCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTCGGT  >  NC_014034/3596844‑3597042
                                                                                                    |                                                                                                   
cAATCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCTCCCCGGCCCGCCGGAGCGAACCGGAAG.cc                                                                                                    >  1:4992642/1‑101 (MQ=255)
cAATCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.cc                                                                                                    >  2:2551136/1‑101 (MQ=255)
 actCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.ccc                                                                                                   <  1:3996340/99‑1 (MQ=255)
  aTCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.cccc                                                                                                  <  2:516255/101‑1 (MQ=255)
   tCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.ccccc                                                                                                 >  1:3467262/1‑101 (MQ=255)
    ccccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.cccccc                                                                                                <  2:1208802/101‑1 (MQ=255)
      ccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.cccccccc                                                                                              <  1:1037007/101‑1 (MQ=255)
       cAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.ccccccccc                                                                                             >  2:4134174/1‑101 (MQ=255)
        aGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.cccccccccc                                                                                            <  1:641401/101‑1 (MQ=255)
           cGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGc                                                                                         >  2:2041146/1‑101 (MQ=255)
            gCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGcc                                                                                        >  2:8611237/1‑101 (MQ=255)
            ccAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGcc                                                                                        <  2:8441634/100‑1 (MQ=255)
             cAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCg                                                                                       >  2:8174804/1‑101 (MQ=255)
             cAACCCCCCCCCCCCCCTCGGCCCGCCCGCGTTGCAGGCGGCAGACGCCGGCCTGCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCg                                                                                       <  1:5143303/101‑1 (MQ=255)
              aacccccccgccccccTCGGCCCGACCGCGTTGCAGGGGGCAGAGACGGGCCTTCCCGCCCCGGCCCGCGGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGa                                                                                      <  1:8997353/98‑1 (MQ=255)
              aaGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGGCGGAGCGAACCGGAAG.CCCCCCCCCCGGcccg                                                                                      >  1:6262799/1‑99 (MQ=255)
              aaGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCGGCCCgg                                                                                      >  1:1898169/1‑100 (MQ=255)
               aGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCgcg                                                                                     >  1:747563/1‑99 (MQ=255)
               aGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAg                                                                                     >  1:1138534/1‑101 (MQ=255)
                  cccccgccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGgcg                                                                                  >  2:2869901/1‑101 (MQ=255)
                   ccccgccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGgcgc                                                                                 >  2:3375291/1‑101 (MQ=255)
                       gccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGAt                                                                             >  1:1198767/1‑101 (MQ=255)
                         cccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG..CCCCCCCCCGGCCGAGGCGCGGAtctc                                                                          <  1:8611479/101‑1 (MQ=255)
                          ccccTCGGCCCGCCCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGAtctc                                                                          <  1:2869999/101‑1 (MQ=255)
                           cccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCg                                                                         <  1:4829126/101‑1 (MQ=255)
                             cTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGcc                                                                       >  1:5806893/1‑101 (MQ=255)
                             cTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCCCGcc                                                                       >  1:8523366/1‑101 (MQ=255)
                                ggCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCg                                                                    <  1:8543422/101‑1 (MQ=255)
                                  cccGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCggg                                                                  >  2:4885500/1‑101 (MQ=255)
                                  cccGACCGCGTTGCAGGCGGCAGAGACCGGCCCTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCggg                                                                  <  2:6764133/101‑1 (MQ=255)
                                     gACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGGGGCGCGGTCCTCGCCCCGGGTgg                                                               >  2:1685925/1‑101 (MQ=255)
                                      aCCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGGGCGGATCTCGCCCCGGGGGgc                                                              >  2:2356468/1‑101 (MQ=255)
                                      aCCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCACCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGgc                                                              >  2:3214108/1‑101 (MQ=255)
                                         gcgTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCgct                                                           >  2:7444088/1‑101 (MQ=255)
                                         gcgTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCgct                                                           <  1:7805047/101‑1 (MQ=255)
                                          ggTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCgctg                                                          <  1:702946/100‑1 (MQ=255)
                                          cgTGGCAGGCGGCAGAGGCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCgctg                                                          <  2:8295733/101‑1 (MQ=255)
                                           gTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCgctgc                                                         >  2:7371795/1‑101 (MQ=255)
                                           gTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCCCGGATTCCGCCCCGGGGGgggccgt                                                         >  1:2258231/1‑98 (MQ=255)
                                             tGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGctgctg                                                       >  1:1570028/1‑101 (MQ=255)
                                             tGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGCCCGAGCCGCGGACCTCCCCCCGGGGGGGgccgcgt                                                       >  1:8020512/1‑99 (MQ=255)
                                              gCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCGGa                                                      >  2:3899648/1‑101 (MQ=255)
                                               cAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGTGGCGCGGATCTCGCCCCCGGGGGCgctgctcac                                                     >  2:6875814/1‑98 (MQ=255)
                                                aGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGCTCCCGCCCCGGGTGGGgctggcgccg                                                    >  2:1288598/1‑95 (MQ=255)
                                                aGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATc                                                    >  2:6105875/1‑101 (MQ=255)
                                                 ggcggcAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTTGCGCTGCTGATCg                                                   <  2:38477/101‑1 (MQ=255)
                                                  gcggcctcggccGCCCTCCCCGCCCCGGCCCGCCGGAGCGACCCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCgg                                                  <  1:2705126/91‑1 (MQ=255)
                                                    ggcAGAGCCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCt                                                <  1:5898353/101‑1 (MQ=255)
                                                      cAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTAt                                              >  1:2005060/1‑101 (MQ=255)
                                                       ggcggccGGCCCTCCCCCCCCGGCCCCCCCGAGGGAACCGGAAGCCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTAt                                              <  1:6172690/96‑1 (MQ=255)
                                                       agagACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGGCGATCGGCTATg                                             >  2:6497615/1‑101 (MQ=255)
                                                       agagACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATg                                             <  1:7689040/101‑1 (MQ=255)
                                                       agagACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGAtagatcgga                                             >  1:321215/1‑93 (MQ=255)
                                                       agagACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG..CCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGt                                            >  2:264834/1‑101 (MQ=255)
                                                        gaggCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGt                                            <  2:6908645/101‑1 (MQ=255)
                                                        gagaCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATTGGCTCTGt                                            >  2:213948/1‑101 (MQ=255)
                                                         agcccGGCCTCCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG..CCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCa                                          <  2:3559257/98‑1 (MQ=255)
                                                         agaCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTc                                           >  1:7342479/1‑101 (MQ=255)
                                                         agaCCGGCCTCCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTc                                           <  1:5710664/101‑1 (MQ=255)
                                                         agaCCGCCCTCCCCGCCCGGGCCCGCCGGAGCGACCCGGAAG..CCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCa                                          <  1:3296603/101‑1 (MQ=255)
                                                          gcccGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCa                                          <  2:1399208/99‑1 (MQ=255)
                                                          gcccGCCCTCCCCCCCCCGGCCCGCCGGACCGAACCGGAAC.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCa                                          <  2:60731/99‑1 (MQ=255)
                                                          gaCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCCATGTcc                                          >  1:2456372/1‑100 (MQ=255)
                                                           cccGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCAc                                         <  1:4885660/100‑1 (MQ=255)
                                                           cccGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCAc                                         <  2:4052965/100‑1 (MQ=255)
                                                             cGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGGGCTGCTGATCGGCTATGTCACGc                                       >  2:2441910/1‑101 (MQ=255)
                                                               gCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCtt                                     <  1:7931765/101‑1 (MQ=255)
                                                               gCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCCCGGATCTCGCCCCGGGGGGCGCTGCTGCTCGGCTATGTCACGcct                                     >  2:4552288/1‑99 (MQ=255)
                                                               gCCTGCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCtt                                     <  2:2258164/101‑1 (MQ=255)
                                                                  ttCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGGTCGGCTATGTACCGCTTGCg                                  >  2:2861036/1‑101 (MQ=255)
                                                                    cccGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGGGCGGAGCTCGCCCCGGGGGGCGCTGCGGATCGGCTACGTCCCGCTGGCGGt                                >  2:5500698/1‑101 (MQ=255)
                                                                    cccGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCACGCTTGCGGt                                >  1:2340860/1‑101 (MQ=255)
                                                                       gCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCt                             <  2:4426703/101‑1 (MQ=255)
                                                                        ccccGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTg                            <  1:8597219/101‑1 (MQ=255)
                                                                        ccccGGCCCGCCGGAGCGAACCGGAAG..CCCCCCCCCGGCCCGGGCGCGGATCTCGCCCCCGGGGGCGCCGCCGATCGGCTATGTCCCGCCTGccggtcctg                           >  1:3542505/1‑93 (MQ=255)
                                                                         cccGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTgg                           <  1:835669/101‑1 (MQ=255)
                                                                          ccGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCC.GCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTgggc                         >  1:6902211/1‑99 (MQ=255)
                                                                           cGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTc                         <  2:6262559/101‑1 (MQ=255)
                                                                           cGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTc                         >  1:5611471/1‑101 (MQ=255)
                                                                           cGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCCCGCCCCGGGGGGGGCGGCTGATCGGCTTTGTCCCGCTTCCGGTTCTgggc                         >  1:5269113/1‑99 (MQ=255)
                                                                            ggCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCg                        >  2:8877470/1‑101 (MQ=255)
                                                                            ggCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCCCCCTTGCGGGTCTGGTCg                        >  2:5361055/1‑101 (MQ=255)
                                                                                   cgGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGGtt                 >  2:7902920/1‑101 (MQ=255)
                                                                                   cgGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCtt                 <  1:2621021/101‑1 (MQ=255)
                                                                                    ggAGGGAGCCGGCAC.CGCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTc                <  1:4502587/101‑1 (MQ=255)
                                                                                        cGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGc            >  2:749961/1‑101 (MQ=255)
                                                                                        cGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGc            >  2:525669/1‑101 (MQ=255)
                                                                                        cGAACCGGAAG..CCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCCCGCTTGCGGTTCTGGTCGGGGGCTTCGGGCt           <  1:2118046/101‑1 (MQ=255)
                                                                                         gAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCt           >  2:1879043/1‑101 (MQ=255)
                                                                                           aCCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGAATTCGCCCCGGGGGTCGCTGCGCATCGCCTTTGCCAGGCGTGCCGTTCTGGTCGGGGGCCCCCGGCttt         >  2:6592548/1‑101 (MQ=255)
                                                                                            ccGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGGGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTg        >  2:9124774/1‑101 (MQ=255)
                                                                                            ccGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTg        <  2:7193088/101‑1 (MQ=255)
                                                                                             cGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTgg       >  2:4187761/1‑101 (MQ=255)
                                                                                             cGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTgg       >  1:921642/1‑101 (MQ=255)
                                                                                              ggAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTTTGGTCGGGGGCTTCGGGCTTTGGt      >  1:5954598/1‑101 (MQ=255)
                                                                                              ggAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGTTGATCGGCTAAGCAACGCTTGCGGTTCGGGGCGGGGGCTTCTGGCTTTggc      >  2:572745/1‑100 (MQ=255)
                                                                                              ggAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGt      >  1:372837/1‑101 (MQ=255)
                                                                                               gAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTc     <  2:1950365/101‑1 (MQ=255)
                                                                                                aaG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTCg    >  2:5632694/1‑101 (MQ=255)
                                                                                                 aG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCGGCTGATCGGCTATGGCACGCTTGCGGTTCTGGGCGGGGGCTTCGGGCTTTGGGCgg   >  2:8459721/1‑101 (MQ=255)
                                                                                                    |                                                                                                   
CAATCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGCTATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTCGGT  >  NC_014034/3596844‑3597042

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 21 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG