Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 568,802 Δ1 bp 3.2% intergenic (‑98/+68) RCAP_rcc00530 ← / ← carB hypothetical protein/carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034568,8020C→. 3.2% 21.4 ‑0.7 ‑0.2308intergenic (‑98/+68)RCAP_rcc00530/carBhypothetical protein/carbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (5/5):  ref base (189/109):  total (194/114)
Fisher's exact test strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.80e-01

Only 100 of 418 total aligned reads displayed.

CATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGCC  >  NC_014034/568702‑568902
                                                                                                      |                                                                                                     
cATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.c                                                                                                       >  1:264970/1‑101 (MQ=255)
 aTTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.cc                                                                                                      >  1:4173494/1‑101 (MQ=255)
   tCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.cccc                                                                                                    <  1:8654431/101‑1 (MQ=255)
   tCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.cccc                                                                                                    >  2:5214702/1‑101 (MQ=255)
   tCCCGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.cccc                                                                                                    <  2:4221922/101‑1 (MQ=255)
     cTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCGGCGGCATCCTG.cccccc                                                                                                  >  2:2374818/1‑101 (MQ=255)
      tGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.ccccccc                                                                                                 >  1:7332648/1‑101 (MQ=255)
       gCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.cccccccc                                                                                                <  2:1072119/101‑1 (MQ=255)
         cTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.ccccccccct                                                                                              <  2:5944174/101‑2 (MQ=255)
         cTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.ccccccccc.c                                                                                             <  1:4364651/101‑1 (MQ=255)
         cTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.ccccccccc.c                                                                                             >  1:5922877/1‑101 (MQ=255)
          tCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.Cg                                                                                            <  1:4634569/101‑1 (MQ=255)
          tCCAGTTTCTGTATGGGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.Cg                                                                                            <  1:4019283/101‑1 (MQ=255)
           ccAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGa                                                                                           >  1:890181/1‑101 (MQ=255)
             ggTTTCGTTATGTAAACGGGGGTGTGGGCCTGGGGCTGGGGGGGGTCAAGGCCCCGCGCGGG.TTGGGAAGGGCGTTGCGGCTTCCTG.CCCCCCCCC.CGACg                                                                                         <  2:2295552/100‑1 (MQ=255)
              gTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACgg                                                                                        >  1:1383769/1‑101 (MQ=255)
               tttCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACggg                                                                                       >  1:5344917/1‑101 (MQ=255)
               tttCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CCACggg                                                                                       >  2:2727108/1‑101 (MQ=255)
                ttCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACgggg                                                                                      >  2:199985/1‑100 (MQ=255)
                 tCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACggggg                                                                                     >  1:1102198/1‑99 (MQ=255)
                 tCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGag                                                                                     >  2:5033657/1‑101 (MQ=255)
                  cTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGagg                                                                                    <  1:3520419/101‑1 (MQ=255)
                  cTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CCCCGGGggg                                                                                    >  1:2783856/1‑101 (MQ=255)
                  cTGTATGTGAACCGGGGCTTACGCCTGTGGCTCCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGagg                                                                                    <  2:6769172/101‑1 (MQ=255)
                   tGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGaggg                                                                                   >  1:4878289/1‑101 (MQ=255)
                    gTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CCACGGGagggc                                                                                  >  1:633907/1‑101 (MQ=255)
                     tATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CCACGGGggggca                                                                                 >  1:2418803/1‑101 (MQ=255)
                      aTGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGagggcag                                                                                <  1:5051252/101‑1 (MQ=255)
                        gtgAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.ACCCCCCCC.CGACGGGagggcaggg                                                                              >  1:5546251/1‑101 (MQ=255)
                         tgcaCCGGGGTTTACGCCTGTGGCTCCTGTGGGGAAATTACCCGCACGGGCTTGTTAA.GGCCCTCCGGCATCCTT.CCCCCCCCC.CGACGGGagggcagggc                                                                             <  1:7627585/98‑1 (MQ=255)
                         tgAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGagggcagggc                                                                             <  1:8046817/101‑1 (MQ=255)
                           aaCCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGggggcagggcgg                                                                           >  1:7165448/1‑99 (MQ=255)
                             ccGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGt                                                                         <  2:8673308/101‑1 (MQ=255)
                              cGGGCCTTACGCCTGTGGGTGCGGTGGGGCAATGACCCGCAGGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGtt                                                                        <  2:7473658/101‑1 (MQ=255)
                               ggggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGCttt                                                                       <  1:8822554/101‑1 (MQ=255)
                               ggggCATACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGGGGGCAGGGCGGGttt                                                                       >  2:4520068/1‑101 (MQ=255)
                                gggCTTACGCCTGTGGCTGCGGTGGGGCAATGGCCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTc                                                                      <  2:5825885/101‑1 (MQ=255)
                                  gCTTACGCCTGTGGCGGGGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCgg                                                                    <  2:1872442/101‑1 (MQ=255)
                                     tACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCCggcg                                                                 >  2:661267/1‑101 (MQ=255)
                                      aCGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGggcgg                                                                >  2:2881641/1‑101 (MQ=255)
                                      aCGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGggcgg                                                                <  1:7376714/101‑1 (MQ=255)
                                       cGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGGGGGCAGGGCTGGTTTCGgggggg                                                               >  1:3026958/1‑100 (MQ=255)
                                         ccTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGCCGGGGGGGGCGGGCTGGGTTCGGGCGGCaa                                                             >  2:3130393/1‑101 (MQ=255)
                                            gtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGCCGGGGGGGCAGGGCTGGTTTCGGGGGGCAAggg                                                          >  1:2032511/1‑100 (MQ=255)
                                               gCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGcc                                                       >  1:7776594/1‑101 (MQ=255)
                                                cTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGccc                                                      <  1:6099965/101‑1 (MQ=255)
                                                   cGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGGTTCGGGCGGCAAGGCGCCcgcg                                                   >  2:6973544/1‑101 (MQ=255)
                                                    ggTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCCACGACGGGAGGGCAGGGCTGGTTTCGGGCGGCACGGCGCCcgcg                                                   >  2:8157480/1‑101 (MQ=255)
                                                    ggTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcgg                                                  >  1:2504382/1‑101 (MQ=255)
                                                    ggTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGGTTCGGGCGGCAAGGCGCCcgcgg                                                  >  2:3329045/1‑101 (MQ=255)
                                                     ggtgggCAATGACCCGCTCGGGCTTTTGAA.GGCGCTGCGGCAGCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggc                                                 <  1:3967565/98‑1 (MQ=255)
                                                     gTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGGTGGTTTCGGGCGGCAAGGCGCCcgcggg                                                 >  1:924544/1‑100 (MQ=255)
                                                     gTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggc                                                 <  1:6865454/101‑1 (MQ=255)
                                                        gggCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcgcg                                              <  1:1159090/101‑1 (MQ=255)
                                                           gaaTGACCCGCAGGGGGTTGGGAA.GGCGCTGCGGCCTCCGG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGcc                                           <  2:7277363/100‑1 (MQ=255)
                                                           cAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGcc                                           <  1:3408884/101‑1 (MQ=255)
                                                           cAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGcc                                           >  2:6045341/1‑101 (MQ=255)
                                                             aTGCCCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGt                                         <  1:3631517/101‑1 (MQ=255)
                                                              tGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGGGGGCAGGGCTGGTTTCGGGGGGCAAGGGCCCCCCGGGGCGGCCggg                                        >  2:6288908/1‑99 (MQ=255)
                                                                ccccGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG..CCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCt                                     <  2:6163788/100‑1 (MQ=255)
                                                                aCCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGcc                                      >  2:7585449/1‑101 (MQ=255)
                                                                 cccGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCt                                     >  1:1537794/1‑101 (MQ=255)
                                                                    gCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGGGCCCGCGGCGCGGCCGGGCCTGCt                                  >  2:7867266/1‑101 (MQ=255)
                                                                    gCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCt                                  <  1:6976077/101‑1 (MQ=255)
                                                                    gCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCt                                  <  1:5248245/101‑1 (MQ=255)
                                                                     cACGGGCTTGTGCA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGGGCGGCCGGGCCCGcct                                 >  1:6505725/1‑99 (MQ=255)
                                                                      aCGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTa                                <  1:903759/101‑1 (MQ=255)
                                                                       cGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTAc                               <  2:264967/101‑1 (MQ=255)
                                                                        gggCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACg                              >  1:8444403/1‑101 (MQ=255)
                                                                         ggCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CCACGGGAGGGCAGGGCTGGTTTCGGGGGGCAAGGCGCCCGCGGCGGGGGCGGGCCTGCTTACGc                             >  1:8294809/1‑101 (MQ=255)
                                                                           cTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGGGGGCCGGGCTGGTTTCGGGGGGGAAGGGGCCCGCGGGGCGGCCGGGCCCGCTTACGccc                           >  1:5150022/1‑100 (MQ=255)
                                                                            ttGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCGGCTTCCGCCTg                          >  2:9009296/1‑101 (MQ=255)
                                                                             tgggAA.GGCCCCGCGGGCTCCTC.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGc                         <  2:3904734/98‑1 (MQ=255)
                                                                              gtgAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCa                        <  2:8844712/101‑1 (MQ=255)
                                                                              gtgAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CG.CGGGGGGGCAGGGGTGGGTTCGGGCGGGCAGGCGCCCGCGGGGCGGCCGGGCCGGCGTTCGCCCGccg                       >  2:8549555/1‑99 (MQ=255)
                                                                              gtgAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.C.CCGGGGGGGCCGGGCCGGGTTTGGGGGGCAAAGGGCCCCCGGGGCGGGCCGGCCTCCTCTCGCCTGccg                       >  2:343635/1‑99 (MQ=255)
                                                                               tgAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAg                       >  1:748178/1‑101 (MQ=255)
                                                                                gAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGc                      <  2:5362846/101‑1 (MQ=255)
                                                                                       gcTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCCGCAGCGGcccg                >  2:8227126/1‑99 (MQ=255)
                                                                                        cTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGGGGCGCGGCCGGGCCTGCTTACGCCTGCAGCGGCCggg               >  1:1719677/1‑100 (MQ=255)
                                                                                         tGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGAc              <  2:6843170/101‑1 (MQ=255)
                                                                                          gCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCCGCGGCCGGCcc             >  2:7407777/1‑101 (MQ=255)
                                                                                           gggCAGCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCg            <  2:2489215/100‑1 (MQ=255)
                                                                                            ggCATCCTGCCCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCg            <  1:3146112/101‑1 (MQ=255)
                                                                                            ggCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGGCCgg           >  2:3821907/1‑100 (MQ=255)
                                                                                              cATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGGccccga         >  2:1762673/1‑97 (MQ=255)
                                                                                               ctCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAt        <  2:4853732/100‑1 (MQ=255)
                                                                                               aTCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAt        >  2:3634506/1‑101 (MQ=255)
                                                                                                tCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAtt       <  1:4053606/101‑1 (MQ=255)
                                                                                                tCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAtt       <  1:9129773/101‑1 (MQ=255)
                                                                                                tCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAtt       <  2:5506084/101‑1 (MQ=255)
                                                                                                 cccgcccccccccc.cGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAtt       <  1:3759166/96‑1 (MQ=255)
                                                                                                 ccTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAttt      <  2:7078139/101‑1 (MQ=255)
                                                                                                 ccTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAttt      <  2:1537747/101‑1 (MQ=255)
                                                                                                  cgg.CCCCCCCCC.CGACAGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTc     <  1:3809880/99‑1 (MQ=255)
                                                                                                  cTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTc     <  1:6187921/101‑1 (MQ=255)
                                                                                                  cTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTc     <  2:1392587/101‑1 (MQ=255)
                                                                                                   tG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACGCCCGCAGCGGCCGGGCCGCGATTTCg    >  1:5693875/1‑101 (MQ=255)
                                                                                                    g.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGc   >  2:7643966/1‑101 (MQ=255)
                                                                                                      ccccccccc.cGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGcc  <  1:661277/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
CATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGCC  >  NC_014034/568702‑568902

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG