Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 580,998 Δ1 bp 0.8% intergenic (+119/‑252) RCAP_rcc00541 → / → RCAP_rcc00542 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034580,9980C→. 0.8% 2.8 0.0 ‑0.7472intergenic (+119/‑252)RCAP_rcc00541/RCAP_rcc00542hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (271/197):  total (273/199)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.99e-01

Only 100 of 502 total aligned reads displayed.

GCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTGAGG  >  NC_014034/580899‑581098
                                                                                                     |                                                                                                    
gcTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGc                                                                                                      <  2:2403392/101‑1 (MQ=255)
 cTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGcc                                                                                                     <  1:170365/101‑1 (MQ=255)
  tGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCg                                                                                                    >  2:7999021/1‑101 (MQ=255)
    cccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaa                                                                                                  <  2:8180556/101‑1 (MQ=255)
         tctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                                                             <  1:1020297/101‑1 (MQ=255)
         tctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                                                             <  2:509078/101‑1 (MQ=255)
           tttCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAg                                                                                           >  2:7059981/1‑101 (MQ=255)
           tttCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAg                                                                                           <  2:1562044/101‑1 (MQ=255)
            ttCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgg                                                                                          <  2:3651511/101‑1 (MQ=255)
                gCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGc                                                                                      <  1:8093525/101‑1 (MQ=255)
                gCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGc                                                                                      <  2:6193392/101‑1 (MQ=255)
                 cTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCa                                                                                     <  2:5550374/101‑1 (MQ=255)
                 cTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCa                                                                                     >  2:5733849/1‑101 (MQ=255)
                   gcgcCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgc                                                                                   >  1:6610579/1‑101 (MQ=255)
                   gcgcCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGAGGCAgg                                                                                   >  1:4217130/1‑100 (MQ=255)
                    cgcCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcg                                                                                  <  1:1507080/101‑1 (MQ=255)
                     gcCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcgc                                                                                 <  2:6482798/101‑1 (MQ=255)
                     cggccaaTTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGATGGGGTTTATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCCGCGcc                                                                                >  1:2815306/6‑101 (MQ=37)
                       cGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccc                                                                               >  2:6569270/1‑101 (MQ=255)
                         aaaTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                                                                             >  2:7778391/1‑101 (MQ=255)
                          aaTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                                                                            >  2:5169677/1‑101 (MQ=255)
                          aaTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                                                                            <  2:1270188/101‑1 (MQ=255)
                          aaTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                                                                            >  1:3120521/1‑101 (MQ=255)
                           aTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                                                                           >  2:6273587/1‑101 (MQ=255)
                                 cccccGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTccc                                                                      >  1:575903/1‑101 (MQ=255)
                                    ccGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtc                                                                   <  1:4862159/101‑1 (MQ=255)
                                      gggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                                                                 >  1:5134577/1‑101 (MQ=255)
                                      gggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                                                                 >  2:202482/1‑101 (MQ=255)
                                      gggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                                                                 >  2:83150/1‑101 (MQ=255)
                                        gATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtgt                                                               >  1:5036949/1‑101 (MQ=255)
                                         atatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg                                                              <  1:8274432/101‑1 (MQ=255)
                                           atTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                                                            >  1:3232791/1‑101 (MQ=255)
                                            tttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGGAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAt                                                           >  1:5954102/1‑101 (MQ=255)
                                            tttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAt                                                           >  1:2900228/1‑101 (MQ=255)
                                            tttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAt                                                           >  1:3720368/1‑101 (MQ=255)
                                             ttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                                                          >  1:7908953/1‑101 (MQ=255)
                                             ttGAGGCAAGATAAACGGGCACCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                                                          >  1:7732585/1‑101 (MQ=255)
                                              tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                                                         >  2:8476337/1‑101 (MQ=255)
                                               gAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcgc                                                        >  1:2347178/1‑101 (MQ=255)
                                                aGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcgcg                                                       <  2:5797391/101‑1 (MQ=255)
                                                aGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcgcg                                                       >  1:8670260/1‑101 (MQ=255)
                                                   cAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa                                                    <  1:456682/101‑1 (MQ=255)
                                                    aaGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAAc                                                   >  1:7295307/1‑101 (MQ=255)
                                                     agataaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACg                                                  <  1:848868/96‑1 (MQ=255)
                                                     agataaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACg                                                  >  1:7439332/6‑101 (MQ=255)
                                                     agataaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACg                                                  >  2:7850915/6‑101 (MQ=255)
                                                      gataaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGt                                                 <  2:9145660/97‑1 (MQ=255)
                                                       ataaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGtt                                                >  2:7258661/4‑101 (MQ=255)
                                                         aaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCa                                              <  2:3236516/100‑1 (MQ=255)
                                                         aaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCa                                              >  2:9046893/2‑101 (MQ=255)
                                                          aaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAg                                             <  2:8764482/101‑1 (MQ=255)
                                                          aaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGCCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAg                                             >  2:4430851/1‑101 (MQ=255)
                                                             gggCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCg                                          >  2:4693338/1‑101 (MQ=255)
                                                             gggCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCg                                          >  1:1208653/1‑101 (MQ=255)
                                                              ggCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCgg                                         <  1:5678420/101‑1 (MQ=255)
                                                              ggCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCgg                                         >  1:6880420/1‑101 (MQ=255)
                                                                cgcCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGGGTGGATCCGGCAACGTTCAGGCGGAt                                       >  2:7357444/1‑101 (MQ=255)
                                                                 gcCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAtt                                      <  2:2502479/101‑1 (MQ=255)
                                                                   cTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAtttt                                    >  2:7476320/1‑101 (MQ=255)
                                                                   cTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAtttt                                    >  1:8907844/1‑101 (MQ=255)
                                                                    tATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtc                                   <  1:1397006/101‑1 (MQ=255)
                                                                    tATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtc                                   <  1:2974070/101‑1 (MQ=255)
                                                                    tATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtc                                   >  1:6048730/1‑101 (MQ=255)
                                                                      tCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtctc                                 <  2:2214475/101‑1 (MQ=255)
                                                                        aCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTccc                               >  1:6247557/1‑101 (MQ=255)
                                                                          cTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTg                             >  2:6691733/1‑101 (MQ=255)
                                                                                gCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCAt                       >  1:6472927/1‑101 (MQ=255)
                                                                                 cAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATc                      <  2:6048973/101‑1 (MQ=255)
                                                                                  aaaTATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCg                     >  1:2122886/1‑101 (MQ=255)
                                                                                   aaTATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATGGc                    >  2:4889190/1‑101 (MQ=255)
                                                                                   aaTATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATGGc                    >  2:2917157/1‑101 (MQ=255)
                                                                                   aaTATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGc                    <  1:1577003/101‑1 (MQ=255)
                                                                                    atatCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCt                   >  1:4411572/1‑101 (MQ=255)
                                                                                     tatCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTg                  <  1:1778931/101‑1 (MQ=255)
                                                                                      atCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGc                 >  1:2620256/1‑101 (MQ=255)
                                                                                      atCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGc                 >  2:2682297/1‑101 (MQ=255)
                                                                                        ccGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCAt               >  2:3816095/1‑101 (MQ=255)
                                                                                         cGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATc              >  2:1806418/1‑101 (MQ=255)
                                                                                         cGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATc              >  1:989575/1‑101 (MQ=255)
                                                                                         cGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATc              >  2:7663157/1‑101 (MQ=255)
                                                                                         cGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATc              >  2:3562201/1‑101 (MQ=255)
                                                                                         cGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATc              >  2:1159256/1‑101 (MQ=255)
                                                                                           gggggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTg            >  2:4168321/1‑101 (MQ=255)
                                                                                           gggggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTg            >  1:1325398/1‑101 (MQ=255)
                                                                                           gggggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTg            >  2:2768225/1‑101 (MQ=255)
                                                                                           gggggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTg            >  1:7193742/1‑101 (MQ=255)
                                                                                             gggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATTGCTGCATCTGGt          >  2:1099493/1‑101 (MQ=255)
                                                                                             gggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGt          <  1:1905971/101‑1 (MQ=255)
                                                                                              ggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTc         <  2:6880135/101‑1 (MQ=255)
                                                                                              ggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTc         <  2:8907438/101‑1 (MQ=255)
                                                                                              ggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTc         >  2:3023780/1‑101 (MQ=255)
                                                                                                tGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGc       <  2:4027683/101‑1 (MQ=255)
                                                                                                tGAGGCCGAAAGCCGAGGGGGAAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGc       >  2:3350046/1‑101 (MQ=255)
                                                                                                 gAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCt      >  1:116311/1‑101 (MQ=255)
                                                                                                 gAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCt      >  1:8756129/1‑101 (MQ=255)
                                                                                                 gAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCt      <  2:8659012/101‑1 (MQ=255)
                                                                                                 gAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGGGTGGATCGCGCAACGTTCAGGCGGACTTTCTCCCTGCGGCATCGCTCCATCTGGTCGCt      >  1:9106653/1‑101 (MQ=255)
                                                                                                  aGGCCGAAAGCCGAGCGGGCAGCGCGGCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTg     <  2:5382903/101‑1 (MQ=255)
                                                                                                   ggCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTGa    >  1:3988393/1‑101 (MQ=255)
                                                                                                   ggCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTGa    <  1:3474472/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
GCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTGAGG  >  NC_014034/580899‑581098

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG