Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 581,001 Δ1 bp 0.8% intergenic (+122/‑249) RCAP_rcc00541 → / → RCAP_rcc00542 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034581,0010A→. 0.8% 2.1 0.0 ‑0.6476intergenic (+122/‑249)RCAP_rcc00541/RCAP_rcc00542hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (273/199):  total (275/201)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.27e-01

Only 100 of 507 total aligned reads displayed.

GCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCC.CGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTGAGGGAT  >  NC_014034/580902‑581101
                                                                                                      |                                                                                                    
gCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGa                                                                                                      >  2:8061571/1‑101 (MQ=255)
 cccgcTCTTTCGGCTGCGCCGAAATTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaa                                                                                                     <  2:552342/101‑1 (MQ=255)
 cccccTCTTTCGGCTGCGCCGAAATTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaa                                                                                                     <  2:8180556/101‑1 (MQ=255)
  ccccTCTTTCGGCTGCGCCGAAATTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                                                                                    >  1:1144806/1‑101 (MQ=255)
   cccTCTTTCGGCTGCGCCGAAATTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAg                                                                                                   >  2:7759853/1‑101 (MQ=255)
      tctTTCGGCTGCGCCGAAATTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                                                                <  1:1020297/101‑1 (MQ=255)
       ctTTCGGCTGCGCCGAAATTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGa                                                                                               >  1:2900074/1‑101 (MQ=255)
           cGGCTGCGCCGAAATTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgggg                                                                                           <  2:4085975/101‑1 (MQ=255)
             gCTGCGCCGAAATTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGc                                                                                         <  1:8093525/101‑1 (MQ=255)
             gCTGCGCCGAAATTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGc                                                                                         <  1:5735261/101‑1 (MQ=255)
               cttcggccaaTTCA.CCCCACGGGATATTTGCGGCAAGGTGAAAGGATGGGGTTTATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgc                                                                                      <  2:1604283/93‑1 (MQ=37)
                gcgcCGAAATTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgc                                                                                      >  1:6610579/1‑101 (MQ=255)
                gcgcCGAAATTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATAACCGGGGGGTGAGGCCGAAAGCCGAGGGGGCatc                                                                                      <  2:378847/101‑3 (MQ=255)
                 tcggccaaTTCACCCCC.CGGGATATTTGCGGCAAGGTGAAAGGATGGGGTTTATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgcgc                                                                                    >  2:3962188/7‑101 (MQ=37)
                     gAAATTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                                                                 <  2:1137967/101‑1 (MQ=255)
                       aagtCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                                                                               <  2:8508482/98‑1 (MQ=255)
                       aaTTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                                                                               >  1:3120521/1‑101 (MQ=255)
                       aaTTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                                                                               >  2:9052222/1‑101 (MQ=255)
                       aaTTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                                                                               >  1:1313660/1‑101 (MQ=255)
                        aTTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                                                                              <  1:8765228/101‑1 (MQ=255)
                        aTTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                                                                              <  2:6427702/101‑1 (MQ=255)
                        aTTCA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                                                                              <  1:4538733/101‑1 (MQ=255)
                           cA.CCCC.CGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                                                                           <  1:3979673/101‑1 (MQ=255)
                               ccc.cGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcg                                                                        >  1:1355387/1‑101 (MQ=255)
                                cc.cGGGATATTTGAGGCAAGATAAACGGGCGCCTNTCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgt                                                                       <  2:6025357/101‑1 (MQ=255)
                                 c.cGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtc                                                                      >  1:8517541/1‑101 (MQ=255)
                                    gggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                                                                    <  1:5026988/101‑1 (MQ=255)
                                    gggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                                                                    >  2:83150/1‑101 (MQ=255)
                                    gggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                                                                    <  1:1893538/101‑1 (MQ=255)
                                      gATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtgt                                                                  >  1:1201559/1‑101 (MQ=255)
                                       atatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGGGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg                                                                 >  1:4204845/1‑101 (MQ=255)
                                       atatTTGAGGCAAGATAAACGGGCGCCTATCACCTGGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg                                                                 >  1:8345587/1‑101 (MQ=255)
                                       atatTTGAGGCAAGATAAACGGGCGCCTATCAACTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAACGCCCCCTTTTCCCGTCGtgtg                                                                 <  1:939651/101‑1 (MQ=38)
                                        tatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg                                                                >  1:1765102/1‑101 (MQ=255)
                                         atTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                                                               >  2:3183578/1‑101 (MQ=255)
                                         atTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                                                               >  1:3232791/1‑101 (MQ=255)
                                          tttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGGAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAt                                                              >  1:5954102/1‑101 (MQ=255)
                                          tttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAt                                                              <  2:5097008/101‑1 (MQ=255)
                                           ttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                                                             >  1:7908953/1‑101 (MQ=255)
                                           ttGAGGCAAGATAAACGGGCACCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                                                             >  1:7732585/1‑101 (MQ=255)
                                            tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                                                            >  2:7469016/1‑101 (MQ=255)
                                            tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                                                            >  1:1656748/1‑101 (MQ=255)
                                            tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                                                            >  2:5781315/1‑101 (MQ=255)
                                            tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                                                            <  1:4310272/101‑1 (MQ=255)
                                             gAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGCTcgc                                                           >  2:1065251/1‑101 (MQ=255)
                                              aGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcgcg                                                          >  2:3434495/1‑101 (MQ=255)
                                               ggCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCgcgc                                                         <  1:1494255/101‑1 (MQ=255)
                                                gCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCa                                                        >  1:5128940/1‑101 (MQ=255)
                                                gCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCa                                                        >  1:5618/1‑101 (MQ=255)
                                                 cAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa                                                       <  2:3612219/101‑1 (MQ=255)
                                                 cAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa                                                       >  1:4743290/1‑101 (MQ=255)
                                                   agataaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACg                                                     >  2:7850915/6‑101 (MQ=255)
                                                     ataaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGtt                                                   >  1:2715141/4‑101 (MQ=255)
                                                       aaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGTGCAGCGCCCCCTTTTCCTGTCGTGTGGATCGCGCAACGTTCa                                                 <  2:4368721/100‑1 (MQ=255)
                                                       aaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCa                                                 >  2:5254271/2‑101 (MQ=255)
                                                        aaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAg                                                <  2:8764482/101‑1 (MQ=255)
                                                         aCGGGCGCCTATCTCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAATCCGAGGGGGCATCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAgg                                               <  2:7158594/101‑1 (MQ=255)
                                                          cGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGc                                              >  2:6969880/1‑101 (MQ=255)
                                                           gggCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCg                                             >  2:4693338/1‑101 (MQ=255)
                                                            ggCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCgg                                            >  1:6880420/1‑101 (MQ=255)
                                                                ccTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAttt                                        <  1:3712777/101‑1 (MQ=255)
                                                                  tATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtc                                      >  1:6048730/1‑101 (MQ=255)
                                                                  tATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtc                                      <  1:2974070/101‑1 (MQ=255)
                                                                   attaCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtct                                     >  2:1408191/4‑101 (MQ=255)
                                                                   aTCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtct                                     >  2:1229007/1‑101 (MQ=255)
                                                                     cACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTcc                                   <  2:7612317/101‑1 (MQ=255)
                                                                     cACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTcc                                   <  2:625121/101‑1 (MQ=255)
                                                                      aCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTccc                                  >  1:6247557/1‑101 (MQ=255)
                                                                       ccTTGGTGCAAAAATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTGTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCt                                 <  1:9075211/101‑1 (MQ=255)
                                                                          tGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGTATTTTCTCCCTGCg                              >  1:2088081/1‑101 (MQ=255)
                                                                          tGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCg                              >  2:2134948/1‑101 (MQ=255)
                                                                           ggTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCgg                             >  1:2551816/1‑101 (MQ=255)
                                                                            gTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGc                            >  2:3304449/1‑101 (MQ=255)
                                                                            gTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGc                            >  2:6178250/1‑101 (MQ=255)
                                                                             tGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCa                           >  1:2312412/1‑101 (MQ=255)
                                                                              gCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCAt                          >  1:6472927/1‑101 (MQ=255)
                                                                               cAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATc                         <  2:735092/101‑1 (MQ=255)
                                                                                 aaTATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATGGc                       >  2:2917157/1‑101 (MQ=255)
                                                                                 aaTATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATGGc                       >  2:4889190/1‑101 (MQ=255)
                                                                                  atatCCGGGGGGTGACGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCt                      <  2:6688021/101‑1 (MQ=255)
                                                                                    atCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGAGGCATCGCTGc                    >  2:2679479/1‑101 (MQ=255)
                                                                                      ccGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCAt                  >  1:1570361/1‑101 (MQ=255)
                                                                                       cGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATc                 >  2:5694740/1‑101 (MQ=255)
                                                                                        ggggggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCt                <  2:2312345/101‑1 (MQ=255)
                                                                                        ggggggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCt                <  2:4552903/101‑1 (MQ=255)
                                                                                         gggggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTg               >  1:7193742/1‑101 (MQ=255)
                                                                                         gggggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTg               >  2:2768225/1‑101 (MQ=255)
                                                                                            ggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTc            <  2:8907438/101‑1 (MQ=255)
                                                                                            ggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTc            >  2:3023780/1‑101 (MQ=255)
                                                                                            ggTCAGGCCGAAAGCCGAGGGGGCGGCGCGCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTgggc            <  2:6763758/101‑3 (MQ=255)
                                                                                                 ggCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTGa       <  1:3474472/101‑1 (MQ=255)
                                                                                                  gCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTGAg      >  2:6566891/1‑101 (MQ=255)
                                                                                                  gCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTGAg      >  1:7436862/1‑101 (MQ=255)
                                                                                                  gCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTGAg      <  2:249639/101‑1 (MQ=255)
                                                                                                   ccGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTGAgg     >  2:1806952/1‑101 (MQ=255)
                                                                                                    cGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTGAggg    <  1:7275595/101‑1 (MQ=255)
                                                                                                    cGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTGAggg    <  1:8693118/101‑1 (MQ=255)
                                                                                                    cGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTGAggg    >  1:8932803/1‑101 (MQ=255)
                                                                                                     gAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTGAGGGa   <  2:8658560/101‑1 (MQ=255)
                                                                                                     gAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTGAGGGa   >  2:9151110/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
GCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCC.CGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCTGGTCGCTGAGGGAT  >  NC_014034/580902‑581101

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG