Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 631 Δ1 bp 0.9% coding (281/1374 nt) dnaA → chromosomal replication initiator protein DnaA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140346310A→. 0.9% 2.5 ‑1.2 ‑0.1467coding (281/1374 nt)dnaAchromosomal replication initiator protein DnaA
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (185/278):  total (188/279)
Fisher's exact test strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.56e-01

Only 100 of 502 total aligned reads displayed.

CTGCGCGAGCTGCGCAATTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCTTTGACAGCTTCGTC  >  NC_014034/531‑731
                                                                                                    |                                                                                                    
cTGCGCGAGCTGCGCAATTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGa                                                                                                      >  2:1353150/1‑101 (MQ=255)
   cgcgAGCTGCGCAATTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGaaaa                                                                                                   >  2:8661018/1‑101 (MQ=255)
    ccgATCTGCGCAATTCCGGCATGGCGGTCGAACGGGTGCCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAg                                                                                                  <  1:8645044/100‑1 (MQ=255)
     cgAGCTGCGCAATTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAgg                                                                                                 >  1:4330095/1‑101 (MQ=255)
     cgAGCTGCGCAATTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAgg                                                                                                 >  1:5679827/1‑101 (MQ=255)
          tGCGCAATTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCt                                                                                            <  1:1789465/101‑1 (MQ=255)
          tGCGCAATTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCt                                                                                            <  2:6115574/101‑1 (MQ=255)
          tGCGCAATTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCt                                                                                            <  2:3704247/101‑1 (MQ=255)
           gcgcAATTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTg                                                                                           <  1:4644524/101‑1 (MQ=255)
           gcgcAATTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAACCCCGGCCGATGCCGGAAAAGGcccccg                                                                                           >  2:2244666/1‑99 (MQ=255)
            cgcAATTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGc                                                                                          <  2:4906773/101‑1 (MQ=255)
             gcAATTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGcc                                                                                         >  2:280650/1‑101 (MQ=255)
              cAATTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGccc                                                                                        <  2:5268410/101‑1 (MQ=255)
              cAATTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGccc                                                                                        <  1:5989810/101‑1 (MQ=255)
               aaTTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGcccc                                                                                       <  1:4808353/101‑1 (MQ=255)
                aTTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCg                                                                                      >  2:2707457/1‑101 (MQ=255)
                 ttCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCgg                                                                                     <  1:1247527/101‑1 (MQ=255)
                  tCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGgc                                                                                    <  1:395419/101‑1 (MQ=255)
                   ccGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGgcc                                                                                   >  2:5919366/1‑100 (MQ=255)
                    cGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGgcgc                                                                                  >  2:5027123/1‑101 (MQ=255)
                    cGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGgcgc                                                                                  >  2:1870170/1‑101 (MQ=255)
                      gCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGACGCCCGGCCGATGCCGGAAAAGGCCCCTGACCCAgggcgc                                                                                >  2:9161633/1‑101 (MQ=255)
                      gCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGgcgcgc                                                                                >  2:8448190/1‑101 (MQ=255)
                        aTGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGTAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCaa                                                                              <  2:4358317/101‑1 (MQ=255)
                        aTGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCaa                                                                              >  2:902852/1‑101 (MQ=255)
                          ggCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAgg                                                                            >  1:3202271/1‑101 (MQ=255)
                           gCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGc                                                                           <  2:6805484/101‑1 (MQ=255)
                           gCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGc                                                                           <  2:1150470/101‑1 (MQ=255)
                            atctCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGcc                                                                          <  1:7403973/98‑1 (MQ=255)
                             ggTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCg                                                                         <  2:2582250/101‑1 (MQ=255)
                             ggTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCg                                                                         >  1:7194946/1‑101 (MQ=255)
                              gTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGc                                                                        <  2:7796039/101‑1 (MQ=255)
                                cGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCaa                                                                      >  2:7910308/1‑101 (MQ=255)
                                 gAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAg                                                                     <  2:4018161/101‑1 (MQ=255)
                                 gAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAg                                                                     >  2:7040856/1‑101 (MQ=255)
                                 gAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAg                                                                     <  1:8504519/101‑1 (MQ=255)
                                 gAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAg                                                                     >  1:7266804/1‑101 (MQ=255)
                                 gAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAg                                                                     <  1:5290023/101‑1 (MQ=255)
                                  aaCGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAgg                                                                    <  2:8228677/101‑1 (MQ=255)
                                   aCGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAggg                                                                   <  2:6931036/101‑1 (MQ=255)
                                     gggTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTc                                                                 >  1:2042832/1‑101 (MQ=255)
                                     gggTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTc                                                                 <  1:2594120/101‑1 (MQ=255)
                                     gggTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTc                                                                 >  1:9031891/1‑101 (MQ=255)
                                       gtagagagccGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGTCAGGCCGATGACGGAAAAGCCCGCCGCCCCGACGCGCAAGGTCGCGAGGGTCac                                                               >  2:2078842/9‑100 (MQ=255)
                                          gCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGa                                                            <  2:8303283/101‑1 (MQ=255)
                                           cGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAg                                                           >  1:4456084/1‑101 (MQ=255)
                                            gATCCGCGTGCCCGAGGGAGCCCCGATGCCCGCGCCGGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAgg                                                          <  2:4310178/101‑1 (MQ=255)
                                            gATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAgg                                                          <  1:6962459/101‑1 (MQ=255)
                                             aTCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGa                                                         >  1:6745191/1‑101 (MQ=255)
                                              tCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGAt                                                        <  1:3481821/101‑1 (MQ=255)
                                               ccGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATc                                                       <  1:3347413/101‑1 (MQ=255)
                                                cgcgTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCa                                                      <  2:6637017/101‑1 (MQ=255)
                                                  cgTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAgg                                                    >  2:9071587/1‑101 (MQ=255)
                                                     gCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGAcc                                                 >  2:3223721/1‑101 (MQ=255)
                                                       ccGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCtt                                               <  2:7658945/101‑1 (MQ=255)
                                                       ccGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCtt                                               <  2:3152625/101‑1 (MQ=255)
                                                         gAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTcc                                             <  1:1919307/101‑1 (MQ=255)
                                                         gAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTcc                                             <  2:5164721/101‑1 (MQ=255)
                                                          ggggGCGTCCGATGCCCGCGCCCGAAGCCCGCCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTccc                                            <  1:2986373/100‑1 (MQ=255)
                                                          ggggACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTccc                                            <  1:8366871/100‑1 (MQ=255)
                                                          cgggACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCTGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTccc                                            <  2:2384531/100‑1 (MQ=255)
                                                          aGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTccc                                            >  1:2908953/1‑101 (MQ=255)
                                                           gggACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCg                                           <  1:5102481/101‑1 (MQ=255)
                                                           gggACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCGGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCg                                           <  1:5864871/101‑1 (MQ=255)
                                                              aCGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCgggg                                        >  1:3116490/1‑101 (MQ=255)
                                                                  ccGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGcgcc                                    <  1:417741/101‑1 (MQ=255)
                                                                      tGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTg                                <  2:3864279/101‑1 (MQ=255)
                                                                      tGCCCGCGCCCGAAGCCCGGCCGATGCCGG.AAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTgg                               <  2:5492431/101‑1 (MQ=255)
                                                                        cccGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGa                              <  2:5679620/101‑1 (MQ=255)
                                                                         ccGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGAt                             <  2:7274328/101‑1 (MQ=255)
                                                                         ccGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGAt                             <  2:1761042/101‑1 (MQ=255)
                                                                           gcgcCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATgc                           <  1:3725/101‑1 (MQ=255)
                                                                             gcCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATgcgc                         >  2:4658423/1‑101 (MQ=255)
                                                                              cccGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATgcgcg                        >  1:2307734/1‑101 (MQ=255)
                                                                               ccGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATgcgcgc                       >  2:6412945/1‑101 (MQ=255)
                                                                               ccGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATgcgcgc                       >  2:8075841/1‑101 (MQ=255)
                                                                                 gAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCtt                     <  2:8600990/101‑1 (MQ=255)
                                                                                 gAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCtt                     >  1:6238715/1‑101 (MQ=255)
                                                                                 gAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCtt                     >  2:3513703/1‑101 (MQ=255)
                                                                                 gAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCtt                     >  1:1163389/1‑101 (MQ=255)
                                                                                  aaGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTc                    <  2:4527760/101‑1 (MQ=255)
                                                                                  aaGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTc                    <  1:498900/101‑1 (MQ=255)
                                                                                   aGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCa                   >  1:8018278/1‑101 (MQ=255)
                                                                                    gCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCAc                  >  1:6459770/1‑101 (MQ=255)
                                                                                       cGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCtt               <  1:3810868/101‑1 (MQ=255)
                                                                                       cGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCtt               <  2:7507764/101‑1 (MQ=255)
                                                                                        ggCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCttt              >  1:7572752/1‑101 (MQ=255)
                                                                                        ggCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCttt              <  2:8806569/101‑1 (MQ=255)
                                                                                         gCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCTTTg             <  1:8311344/101‑1 (MQ=255)
                                                                                           cGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCTTTGAc           >  2:714847/1‑101 (MQ=255)
                                                                                           cGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCTTTGAc           <  1:5397771/101‑1 (MQ=255)
                                                                                            gATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCTTTGACa          <  1:1012137/101‑1 (MQ=255)
                                                                                             aTGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCTTTGACAg         <  1:7110822/101‑1 (MQ=255)
                                                                                             aTGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCTTTGACAg         <  1:5561760/101‑1 (MQ=255)
                                                                                              tGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCTTTGACAGc        <  1:1537056/101‑1 (MQ=255)
                                                                                                 cGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCCCCTTTGACGGCTtc     <  2:412599/101‑1 (MQ=255)
                                                                                                 cGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCTTTGACAGCTtc     <  2:5580552/101‑1 (MQ=255)
                                                                                                 cGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCTTTGACAGCTtc     >  1:4101605/1‑101 (MQ=255)
                                                                                                   gAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCTTTGACAGCTtcgt   >  1:7719703/1‑101 (MQ=255)
                                                                                                    aaaaGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCTTTGACAGCTtcgtc  >  2:1596284/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CTGCGCGAGCTGCGCAATTCCGGCATGGCGGTCGAACGGGTGGCGATCCGCGTGCCCGAGGGACGTCCGATGCCCGCGCCCGAAGCCCGGCCGATGCCGGAAAAGGCCCCTGCCCCGGCGCGCAAGGCCGCAAGGGTCAGCGAGGATCAGGACCTTCCCGGGGCGCCGCTGGATGCGCGCTTCACCTTTGACAGCTTCGTC  >  NC_014034/531‑731

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG