Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 665,675 Δ1 bp 0.9% coding (778/1119 nt) RCAP_rcc00619 → ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034665,6750G→. 0.9% 2.9 ‑0.5 ‑0.0441coding (778/1119 nt)RCAP_rcc00619ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (223/213):  total (226/215)
Fisher's exact test strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.50e-01

Only 100 of 497 total aligned reads displayed.

CTCGGGGGCGGTGATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCTTCTGGTCATTCCGGT  >  NC_014034/665575‑665775
                                                                                                    |                                                                                                    
ctcgGGGGCGGTGATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCg                                                                                                      <  1:7474754/101‑1 (MQ=255)
  cgGGGGCGGTGATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCggg                                                                                                    <  1:6321820/101‑1 (MQ=255)
  cgGGGGCGGTGATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCggg                                                                                                    >  1:646141/1‑101 (MQ=255)
  cgGGGGCGGTGATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCggg                                                                                                    >  2:4960728/1‑101 (MQ=255)
     gggCGGTGATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCgggggg                                                                                                 >  1:4145661/1‑101 (MQ=255)
     gggCGGTGATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGGGGTCGAGGGGGCGATGGGAGTTCCGGTGGCGGGCTGGCTTTGGCTTTATGCAgtgggg                                                                                                 >  1:2902009/1‑101 (MQ=255)
       gCGGTGATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGggc                                                                                               <  1:6045313/101‑1 (MQ=255)
       gCGGTGATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGggc                                                                                               >  2:3111504/1‑101 (MQ=255)
        cGGTGATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCggggggggg                                                                                              >  1:4216852/1‑99 (MQ=255)
         ggTGATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGggcgg                                                                                             <  1:4833959/101‑1 (MQ=255)
         ggTGATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGggcgg                                                                                             <  1:178795/101‑1 (MQ=255)
         ggTGATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGggcgg                                                                                             >  2:6190035/1‑101 (MQ=255)
           tGATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGgcggcg                                                                                           <  1:6190274/101‑1 (MQ=255)
           tGATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGAGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGgcggcg                                                                                           <  1:7766026/101‑1 (MQ=255)
            gATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGa                                                                                          >  2:465680/1‑101 (MQ=255)
               tcgtttgtccTCGCGCTTCAGCCTGGGCGTGGTTGTCCAGGTGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCt                                                                                       <  1:8929871/93‑1 (MQ=255)
                cTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTa                                                                                      >  2:3620606/1‑101 (MQ=255)
                cTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTa                                                                                      >  2:671012/1‑101 (MQ=255)
                cTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTa                                                                                      <  2:8838753/101‑1 (MQ=255)
                 ttCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTAt                                                                                     >  2:4738964/1‑101 (MQ=255)
                 ttCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTAt                                                                                     >  2:7036053/1‑101 (MQ=255)
                 ttCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTAt                                                                                     <  2:7998917/101‑1 (MQ=255)
                  tCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTAtg                                                                                    >  1:4446116/1‑101 (MQ=255)
                  tCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTAtg                                                                                    >  1:6632170/1‑101 (MQ=255)
                  tCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGAGATCTAtg                                                                                    >  1:9174107/1‑101 (MQ=255)
                   cTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTAtgg                                                                                   >  2:1861428/1‑100 (MQ=255)
                   cTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCC.GGGGGGCGGCGGTCTAtgtg                                                                                  >  1:8658491/1‑101 (MQ=255)
                    tGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTAtgtg                                                                                  <  2:8408761/101‑1 (MQ=255)
                    tGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTAtgtg                                                                                  <  1:107761/101‑1 (MQ=255)
                          cGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTggtggcg                                                                            <  1:3786767/101‑1 (MQ=255)
                            ctctTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTggtggcggt                                                                          <  2:7726398/101‑1 (MQ=255)
                            ctctTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTggtggcggt                                                                          <  1:828915/101‑1 (MQ=255)
                             tctTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTggtggcggtg                                                                         >  1:3580122/1‑101 (MQ=255)
                             tctTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTggtggcggtg                                                                         <  1:1238242/101‑1 (MQ=255)
                               ttCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTggtggcggtggc                                                                       <  1:527193/101‑1 (MQ=255)
                                 cAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGTGGCGGCGATCTATGTGGTGGCGGTGGCCa                                                                     <  2:5270095/101‑1 (MQ=255)
                                    ccTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGcc                                                                  <  1:4531885/101‑1 (MQ=255)
                                     cTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCt                                                                 <  2:7619694/101‑1 (MQ=255)
                                     cTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCt                                                                 >  1:8492591/1‑101 (MQ=255)
                                      tGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTg                                                                <  2:5131675/101‑1 (MQ=255)
                                      tGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTg                                                                >  1:6700412/1‑101 (MQ=255)
                                      tGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTg                                                                <  1:8232381/101‑1 (MQ=255)
                                       ggTCGTGGTGGTCGAGGGGGCGATGGGGGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTgg                                                               >  2:6006144/1‑101 (MQ=255)
                                       ggTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTgg                                                               <  1:4302766/101‑1 (MQ=255)
                                           gtggtgGTCGCGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCt                                                           <  2:2391976/101‑1 (MQ=255)
                                           gtggtgGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCt                                                           <  1:3724065/101‑1 (MQ=255)
                                            tggtggTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTg                                                          <  2:4010484/101‑1 (MQ=255)
                                             ggtggtCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGc                                                         <  2:3468748/101‑1 (MQ=255)
                                               tggtCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCtt                                                       <  2:339299/101‑1 (MQ=255)
                                               tggtCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCtt                                                       <  2:8664416/101‑1 (MQ=255)
                                                ggtCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTc                                                      <  2:6921931/101‑1 (MQ=255)
                                                 gtCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCt                                                     <  2:1174769/101‑1 (MQ=255)
                                                   cGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTgg                                                   >  2:5727086/1‑101 (MQ=255)
                                                   cGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCC.GGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGc                                                  <  2:3756386/101‑1 (MQ=255)
                                                    gAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGc                                                  >  2:4344194/1‑101 (MQ=255)
                                                    gAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGc                                                  >  2:198854/1‑101 (MQ=255)
                                                      gggggCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGcgt                                                <  1:8780271/101‑1 (MQ=255)
                                                       ggggCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGcgtc                                               <  1:2532949/101‑1 (MQ=255)
                                                       ggggCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGcgtc                                               >  1:1974881/1‑101 (MQ=255)
                                                          gCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGtt                                            <  2:2154892/101‑1 (MQ=255)
                                                            ggtGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGGGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCa                                          <  1:724187/99‑1 (MQ=255)
                                                              tGGGCGTGCCGGTGGCGGGCTCGCTTGGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCAcc                                        <  2:2917664/101‑1 (MQ=255)
                                                               gggCGTTCCGGTGGCGGGCTCCCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGGCTGCTTCTGGCGTCGTTCGccc                                       >  1:7353024/1‑101 (MQ=255)
                                                                   gTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGAc                                   >  2:3016144/1‑101 (MQ=255)
                                                                   gTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGAc                                   <  2:8194986/101‑1 (MQ=255)
                                                                    ttCCGGTGGCGGGCTCGCTTTGGCTTTATGCCTGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACg                                  <  1:7023284/101‑1 (MQ=255)
                                                                        ggTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATgg                              <  2:8925980/101‑1 (MQ=255)
                                                                        ggTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATgg                              <  1:7635286/101‑1 (MQ=255)
                                                                           ggCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGc                           <  1:4739113/101‑1 (MQ=255)
                                                                           ggCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTCCACCCGGACGATGGGGc                           >  1:1384292/1‑101 (MQ=255)
                                                                            gCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCa                          <  1:804762/101‑1 (MQ=255)
                                                                             cGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCaa                         >  1:1519172/1‑101 (MQ=255)
                                                                             cGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGACTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCaa                         <  2:4445989/101‑1 (MQ=255)
                                                                              gggCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAAt                        >  1:2278840/1‑101 (MQ=255)
                                                                              gggCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAAt                        <  1:2719171/101‑1 (MQ=255)
                                                                              gggCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTCCACCCGGACGATGGCGCAAt                        >  2:4752479/1‑101 (MQ=255)
                                                                               ggCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGccctt                       >  1:2113630/1‑97 (MQ=255)
                                                                                 gtCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCg                     <  1:5856640/100‑1 (MQ=255)
                                                                                  tCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCCCCCGGACGATGGGGCAATTCgg                    <  2:9108324/101‑1 (MQ=255)
                                                                                   cGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCggg                   >  1:3242760/1‑101 (MQ=255)
                                                                                   cGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCggg                   >  2:1274678/1‑101 (MQ=255)
                                                                                      tttGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCtt                >  2:5431229/1‑101 (MQ=255)
                                                                                      tttGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCtt                >  1:3989582/1‑101 (MQ=255)
                                                                                      tttGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCtt                >  2:1529126/1‑101 (MQ=255)
                                                                                        tGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCTTCt              <  2:587105/101‑1 (MQ=255)
                                                                                         ggCTTTATGCCGGGGGGGCGGCGATCTTTGTGGTGGCGGTGGCCGGCCTGGGACTGCTTCTGGCGGCCTTCACCCGGACGATGGGGCAATTCGGGCTTCTg             >  2:7404299/1‑101 (MQ=255)
                                                                                           cTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCTTCTGGt           >  1:5733723/1‑101 (MQ=255)
                                                                                            tttATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCTTCTGGTc          >  1:3455575/1‑101 (MQ=255)
                                                                                             ttATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCTTCTGGTCa         >  2:6542515/1‑101 (MQ=255)
                                                                                             ttATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCTTCTGGTCa         <  2:4127104/101‑1 (MQ=255)
                                                                                             ttATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCTTCTGGCCa         >  2:7161010/1‑101 (MQ=255)
                                                                                              tATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCTTCTGGTCAt        >  2:1765936/1‑101 (MQ=255)
                                                                                              tATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCTTCTGGTCAt        <  2:2585414/101‑1 (MQ=255)
                                                                                              tATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCTTCTGGTCAt        >  1:5107187/1‑101 (MQ=255)
                                                                                               aTGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCTTCTGGTCAtt       <  1:7944465/101‑1 (MQ=255)
                                                                                                tGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCTTCTGGTCATTc      <  1:1149993/101‑1 (MQ=255)
                                                                                                 gCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTGGGGCTTCTGGTCATTcc     <  1:5454645/101‑1 (MQ=255)
                                                                                                 gCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCTTCTGGCCATTcc     >  1:678258/1‑101 (MQ=255)
                                                                                                  gcGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCTTCTGGTCATTCCg    <  2:8697014/100‑1 (MQ=255)
                                                                                                    gggggggCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCTTCTGGTCATTCCGGt  <  2:2736239/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CTCGGGGGCGGTGATCCTTCTGGCCTCGCTCTTCAGCCTGGTCGTGGTGGTCGAGGGGGCGATGGGCGTTCCGGTGGCGGGCTCGCTTTGGCTTTATGCCGGGGGGGCGGCGATCTATGTGGTGGCGGTGGCCAGCCTGGGTCTGCTTCTGGCGTCGTTCACCCGGACGATGGGGCAATTCGGGCTTCTGGTCATTCCGGT  >  NC_014034/665575‑665775

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG