Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 735,234 Δ1 bp 2.6% intergenic (‑140/‑345) bchF ← / → ppaA 2‑vinyl bacteriochlorophyllide hydratase/regulatory protein PpaA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034735,2340C→. 2.6% 22.6 ‑2.6 ‑0.2422intergenic (‑140/‑345)bchF/ppaA2‑vinyl bacteriochlorophyllide hydratase/regulatory protein PpaA
Reads supporting (aligned to +/- strand):  new base (3/8):  ref base (229/181):  total (233/189)
Fisher's exact test strand distribution p-value = 7.09e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.75e-01

Only 100 of 507 total aligned reads displayed.

AAAACTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGCGG  >  NC_014034/735134‑735334
                                                                                                    |                                                                                                    
aaaaCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGc                                                                                                      >  2:3874510/1‑101 (MQ=255)
aaaaCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGc                                                                                                      <  2:7848189/101‑1 (MQ=255)
 aaaCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGcc                                                                                                     >  1:8433621/1‑101 (MQ=255)
 aaaCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGcc                                                                                                     >  1:3042837/1‑101 (MQ=255)
 aaaCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTCGATGCAGCGGcc                                                                                                     >  1:9120703/1‑101 (MQ=255)
    cTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGccccc                                                                                                  >  1:3870130/1‑101 (MQ=255)
    cTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGccccc                                                                                                  <  2:1493043/101‑1 (MQ=255)
      ggTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGcgccccc                                                                                                <  1:3999727/101‑1 (MQ=255)
      ggTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGccccccc                                                                                                <  1:6382443/101‑1 (MQ=255)
       gTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGcccccccc                                                                                               >  2:5050666/1‑101 (MQ=255)
       gTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGACCTTGGGCGCAAGGGCGCCTTGATGCAGCGGcccccccc                                                                                               >  2:4225334/1‑101 (MQ=255)
        tCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGccccccccc                                                                                              <  2:8138359/101‑1 (MQ=255)
         cTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCg                                                                                             >  1:2879820/1‑101 (MQ=255)
            gTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCt                                                                                          >  1:7625459/1‑101 (MQ=255)
             tCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTa                                                                                         >  2:4854591/1‑101 (MQ=255)
             tCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTa                                                                                         <  1:119795/101‑1 (MQ=255)
               aCGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTaaa                                                                                       >  2:3201149/1‑101 (MQ=255)
               aCGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTaaa                                                                                       <  1:72277/101‑1 (MQ=255)
                cGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAAc                                                                                      <  2:6503345/101‑1 (MQ=255)
                 gagaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAAcc                                                                                     >  2:841135/1‑101 (MQ=255)
                  agaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACcg                                                                                    >  2:5776807/1‑101 (MQ=255)
                   gaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACcgc                                                                                   <  1:8177372/101‑1 (MQ=255)
                   gaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACcgc                                                                                   >  1:7867402/1‑101 (MQ=255)
                    aaaGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACcgcg                                                                                  <  2:7695290/101‑1 (MQ=255)
                    aaaGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACcgcg                                                                                  >  1:5460540/1‑101 (MQ=255)
                         gtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTccc                                                                             >  1:1337316/1‑99 (MQ=255)
                         gtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCAc                                                                             >  1:628090/1‑101 (MQ=255)
                         gtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCAc                                                                             <  2:6577625/101‑1 (MQ=255)
                          tCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACg                                                                            >  2:3038989/1‑101 (MQ=255)
                             aTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCCCGGCCCCGAcc                                                                         >  1:6041719/1‑100 (MQ=255)
                               gAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCg                                                                       >  1:6554773/1‑101 (MQ=255)
                                atctcTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGt                                                                      <  2:8544753/97‑1 (MQ=255)
                                    cTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGtagat                                                                  >  1:8545138/1‑97 (MQ=255)
                                     ttACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCggg                                                                 >  2:3706970/1‑101 (MQ=255)
                                     ttACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCggg                                                                 >  1:3167739/1‑101 (MQ=255)
                                     ttACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCggg                                                                 >  1:8694034/1‑101 (MQ=255)
                                      tACACTCAGGGGTGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGc                                                                >  2:6954055/1‑101 (MQ=255)
                                      tACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGc                                                                >  1:4745963/1‑101 (MQ=255)
                                        cacTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAg                                                              <  1:6747583/101‑1 (MQ=255)
                                         ccTCAGGGGCGGGCCGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGa                                                             <  2:8269648/100‑1 (MQ=255)
                                            cAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGGCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAc                                                          >  2:4443515/1‑101 (MQ=255)
                                            cAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAc                                                          >  1:8959579/1‑101 (MQ=255)
                                            cAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAc                                                          <  1:2246006/101‑1 (MQ=255)
                                            cAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAc                                                          <  1:1997980/101‑1 (MQ=255)
                                             aGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAcc                                                         >  1:4146180/1‑101 (MQ=255)
                                             aGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAcc                                                         <  1:2611103/101‑1 (MQ=255)
                                             aGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCCGGCAGAAAcc                                                         >  2:3343427/1‑101 (MQ=255)
                                                  cGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggcg                                                    >  1:2506857/1‑101 (MQ=255)
                                                   gggACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggcgg                                                   <  2:6631704/101‑1 (MQ=255)
                                                   gggACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggcgg                                                   >  2:8853955/1‑101 (MQ=255)
                                                     gACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCCCGCCCCGCCCGGGCAGAAACCgggcgggg                                                 >  1:3515298/1‑101 (MQ=255)
                                                     gACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCgggcgggg                                                 <  1:7081534/101‑1 (MQ=255)
                                                      aCGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAAACCCGGGAACGCCTCATCCGGGCAGAAAACaggctgggg                                                >  2:1288744/1‑100 (MQ=255)
                                                       cGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCgggcggggcg                                               >  1:3759287/1‑101 (MQ=255)
                                                       cGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCgggcggggcg                                               >  1:5574695/1‑101 (MQ=255)
                                                        gAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGggcggggggg                                              >  1:4894363/1‑101 (MQ=255)
                                                        gAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGggcggggcgg                                              <  2:7123546/101‑1 (MQ=255)
                                                        gAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGggcggggcgg                                              >  2:2609092/1‑101 (MQ=255)
                                                        gAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGggcggggcgg                                              >  1:2422467/1‑101 (MQ=255)
                                                        gAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGggcggggcgg                                              >  2:478066/1‑101 (MQ=255)
                                                         aaaCGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGgcggggcggg                                             >  1:5650506/1‑101 (MQ=255)
                                                             gccgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATgc                                         <  2:7633136/101‑1 (MQ=255)
                                                             gccgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATgc                                         <  1:2716380/101‑1 (MQ=255)
                                                                 cTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTGCGCCt                                     >  2:2002305/1‑101 (MQ=255)
                                                                 cTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCt                                     >  2:1675817/1‑101 (MQ=255)
                                                                   gCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGCAACCGGGCGGGGCGGGGCGCGCCctt                                   >  2:4604905/1‑101 (MQ=255)
                                                                   gCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCttt                                   <  2:8693629/101‑1 (MQ=255)
                                                                    cccTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCtttt                                  <  2:9081513/101‑1 (MQ=255)
                                                                    cccTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCtttt                                  <  1:3312628/101‑1 (MQ=255)
                                                                      cTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTtctc                               >  1:5241453/1‑101 (MQ=255)
                                                                       ttGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTGCGCCTTTtctc                               >  1:4554373/1‑101 (MQ=255)
                                                                       ttGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTtctc                               >  1:1637918/1‑101 (MQ=255)
                                                                        tGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCa                              <  2:3481688/101‑1 (MQ=255)
                                                                        tGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCa                              <  2:7491354/101‑1 (MQ=255)
                                                                        ggcgcgaAGGGGCGCCTGGATGCAGCGG.CCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCaa                             <  2:7956503/100‑1 (MQ=255)
                                                                          cgcgcAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAAc                            <  1:4688996/101‑1 (MQ=255)
                                                                               aaGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGGGCCCTTTCTCTACCCGGc                       >  2:2462275/1‑101 (MQ=255)
                                                                                aGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTGCGCCTTTTCTCAACCTGGcc                      >  2:5646290/1‑101 (MQ=255)
                                                                                 gggCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGccc                     >  1:434671/1‑101 (MQ=255)
                                                                                   gcgcCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGt                   <  1:1022144/101‑1 (MQ=255)
                                                                                   gcgcCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGt                   <  1:7436769/101‑1 (MQ=255)
                                                                                    cgcCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTa                  <  1:5084234/101‑1 (MQ=255)
                                                                                     gcCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTAt                 >  2:1843903/1‑101 (MQ=255)
                                                                                     gcCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTAt                 <  2:4062446/101‑1 (MQ=255)
                                                                                     gcCTTGATGCAGAGGCCCCGCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTAt                 <  2:595064/101‑1 (MQ=255)
                                                                                      ccTTGATGCAGCGTCCCCCCCCCGGCTAACCCGCGGTCACGTCTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATc                <  2:1121373/101‑1 (MQ=255)
                                                                                      ccTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATc                >  1:4406105/1‑101 (MQ=255)
                                                                                        ttGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCtt              <  2:5265694/101‑1 (MQ=255)
                                                                                        ttGATGCAGCGG.CCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTg             >  1:5843954/1‑101 (MQ=255)
                                                                                         tGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTg             <  2:5529139/101‑1 (MQ=255)
                                                                                         tGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTg             >  1:7704593/1‑101 (MQ=255)
                                                                                            tGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgt          <  2:6153610/101‑1 (MQ=255)
                                                                                            ggCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgt          <  2:731162/100‑1 (MQ=255)
                                                                                             gCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgtc         <  2:2873185/101‑1 (MQ=255)
                                                                                             gCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgtc         <  1:8045462/101‑1 (MQ=255)
                                                                                              cAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgtcg        <  2:2071181/101‑1 (MQ=255)
                                                                                              cAGAGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTGCGCCTTTTCTCAAGCTGGCACGTATCTTGcgtcg        >  1:8807992/1‑101 (MQ=255)
                                                                                                 cGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGGCGTgg     >  2:3769938/1‑101 (MQ=255)
                                                                                                   gCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGCg   <  2:1027312/101‑1 (MQ=255)
                                                                                                    cccccccccGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGCgg  >  2:6320716/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
AAAACTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGCGG  >  NC_014034/735134‑735334

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG