Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 826,038 Δ1 bp 2.2% coding (410/672 nt) thiN → thiamine pyrophosphokinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034826,0380C→. 2.2% 12.4 ‑2.0 ‑0.3324coding (410/672 nt)thiNthiamine pyrophosphokinase
Reads supporting (aligned to +/- strand):  new base (2/5):  ref base (185/132):  total (187/137)
Fisher's exact test strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.42e-01

Only 100 of 401 total aligned reads displayed.

GGGCTGCGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCGGGCGCTCGACCGGGCTGCGCTGGCCGATC  >  NC_014034/825938‑826138
                                                                                                    |                                                                                                     
gggGTGCGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCgcgc                                                                                                       >  1:7215231/1‑101 (MQ=255)
 ggCTGCGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGcc                                                                                                      <  1:4077327/101‑1 (MQ=255)
 ggCTGCGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGGGATCTTTGGCGcc                                                                                                      >  1:4281688/1‑101 (MQ=255)
  gCTGCGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGccc                                                                                                     <  1:3047702/101‑1 (MQ=255)
   cTGCGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGcccc                                                                                                    <  2:8685525/101‑1 (MQ=255)
    tGCGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGccccc                                                                                                   <  1:4476221/101‑1 (MQ=255)
      cGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGccccccc                                                                                                 <  2:6522464/101‑1 (MQ=255)
      cGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGccccccc                                                                                                 >  2:2323333/1‑101 (MQ=255)
       ggCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGcccccccc                                                                                                >  1:2740359/1‑101 (MQ=255)
       ggCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGcccccccc                                                                                                >  2:3940101/1‑101 (MQ=255)
       ggCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTGCGCGcccccccc                                                                                                <  1:8182065/101‑1 (MQ=255)
         cTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCg                                                                                              >  1:8701798/1‑101 (MQ=255)
         cTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCg                                                                                              >  1:6714305/1‑101 (MQ=255)
         cTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGGGATCTTTCGCGCCCCCCCCCg                                                                                              >  1:6198956/1‑101 (MQ=255)
         cTTGATCACACGCTGGCGGCGCTGACCGAACCGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCg                                                                                              <  1:1699766/101‑1 (MQ=255)
           tGATCACTCGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGc                                                                                            >  2:3377593/1‑101 (MQ=255)
            gATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCt                                                                                           >  1:1151127/1‑101 (MQ=255)
             aTCCCACGCCGGCGGCGCTGACCGAACTGCCCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTg                                                                                          <  1:5381202/101‑1 (MQ=255)
              tCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTgg                                                                                         >  2:6286707/1‑101 (MQ=255)
               cacaCGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGAGCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGgc                                                                                        <  2:7416688/101‑1 (MQ=255)
                 cacGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGgcgc                                                                                      <  2:5338832/101‑1 (MQ=255)
                  acGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCt                                                                                     <  1:541132/101‑1 (MQ=255)
                     cTGGGGGCGCTGACCGAACTGGCCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGa                                                                                  <  2:4572752/101‑1 (MQ=255)
                     cTGGCGGCGCTGACCGAACGGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGa                                                                                  <  2:9003506/101‑1 (MQ=255)
                      tGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGAt                                                                                 >  2:8603104/1‑101 (MQ=255)
                       ggcggcGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATc                                                                                <  2:3626446/101‑1 (MQ=255)
                       ggcggcGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATc                                                                                >  1:1008287/1‑101 (MQ=255)
                       ggcggcGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCG.CCCCCCCCGGCTGGCGCTGGATCt                                                                               <  1:1826193/101‑1 (MQ=255)
                         cggcgCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTg                                                                              <  1:8647990/101‑1 (MQ=255)
                          ggcgCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGc                                                                             >  2:5475957/1‑101 (MQ=255)
                           gcgcTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGcc                                                                            >  1:38216/1‑101 (MQ=255)
                             gcTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCt                                                                          >  1:7882600/1‑101 (MQ=255)
                              tctacggAACTGACCACGCGGCCCGGGCAGATCCTGGTCCTGGTCCCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCtt                                                                         <  2:4285246/95‑1 (MQ=255)
                              gtcgcgtgaCTGGCCACGCGGCCCGGGCAGCTCGGGATCCTGCTCGCCGAGGACGAGGTGATCTTTCGCACCCCCCCCCGGCTGGCGCTGGATCTGCCCtt                                                                         <  2:8449718/93‑1 (MQ=255)
                              cTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCTTTGGGGTCGAGGCTATCTTTCGTACCCCCCCCCGGCAAGAGCTGGCTTTGCCCtt                                                                         >  1:5866909/1‑101 (MQ=255)
                              cTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCtt                                                                         <  2:8915350/101‑1 (MQ=255)
                              cTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGACCTTcccct                                                                         >  1:6988505/1‑99 (MQ=255)
                              cTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCCGGCGCTGGATCTGCCCtt                                                                         >  2:1407319/1‑101 (MQ=255)
                              cTGACCGAACTGACCACGCGGCCCGGGCAGATCGGGATCATGATCGCCGAGGACGAGGAGATCTTTCTCGCCCCCCCCCGGACGGCGCTGGATCCGCCCtt                                                                         >  2:1646998/1‑101 (MQ=255)
                                gACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTgg                                                                       <  1:2108101/101‑1 (MQ=255)
                                 aCCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGGTCTGCCCTTggg                                                                      >  2:7758412/1‑101 (MQ=255)
                                  ccGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGc                                                                     <  2:4949168/101‑1 (MQ=255)
                                  ccGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGc                                                                     <  1:8394909/101‑1 (MQ=255)
                                   cGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCa                                                                    >  2:5645718/1‑101 (MQ=255)
                                    gAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCAc                                                                   >  2:7101568/1‑101 (MQ=255)
                                    gAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCAc                                                                   <  2:1128811/101‑1 (MQ=255)
                                     aaCTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCAcg                                                                  >  1:2026043/1‑101 (MQ=255)
                                       cTGGCCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCAcgcg                                                                >  1:8801946/1‑101 (MQ=255)
                                        tGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCgg                                                               <  2:6028700/101‑1 (MQ=255)
                                        tGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCgg                                                               >  1:963020/1‑101 (MQ=255)
                                         gACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGc                                                              >  1:4575596/1‑101 (MQ=255)
                                         gACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGc                                                              >  2:8227439/1‑101 (MQ=255)
                                         gACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGc                                                              >  2:1472875/1‑101 (MQ=255)
                                         gACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCCGGATCTGCCCTTGGGCACGCGGc                                                              >  2:9038953/1‑101 (MQ=255)
                                          aCCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCG.CCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTg                                                            >  2:3155610/1‑101 (MQ=255)
                                            cACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGt                                                           >  1:2572796/1‑101 (MQ=255)
                                             ccgcgGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTc                                                          <  2:4154536/100‑1 (MQ=255)
                                                 ggCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTg                                                      >  1:3677917/1‑101 (MQ=255)
                                                 ggCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGAGCTGCCCTTGGGCACGCGGCTGGCGcgg                                                      >  1:7336900/1‑99 (MQ=255)
                                                    ccGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCAt                                                   >  1:8044571/1‑101 (MQ=255)
                                                     cGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATc                                                  <  1:2290759/101‑1 (MQ=255)
                                                         cAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGAt                                              >  2:1819043/1‑101 (MQ=255)
                                                           gATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATgg                                            >  2:2668768/1‑101 (MQ=255)
                                                           gATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCG.CCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATggg                                           >  2:8392106/1‑101 (MQ=255)
                                                            aTCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATggg                                           >  1:4869973/1‑101 (MQ=255)
                                                              cGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTGGCTGCATCCGATGGGAc                                         <  2:4052398/101‑1 (MQ=255)
                                                               gTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGAcc                                        <  2:5148060/101‑1 (MQ=255)
                                                                tGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCg                                       >  1:7779956/1‑101 (MQ=255)
                                                                  aTCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGGGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGCTGGGACCGGc                                     >  2:7240287/1‑101 (MQ=255)
                                                                  aTCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGc                                     <  2:2548914/101‑1 (MQ=255)
                                                                  aTCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGc                                     <  1:1599281/101‑1 (MQ=255)
                                                                    ccTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGcgc                                   >  2:3017307/1‑99 (MQ=255)
                                                                    ccTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.tt                                  <  2:701656/101‑1 (MQ=255)
                                                                      tGATCGCCGGGGCCGAGGTGATCTTTCGCCCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTcc                                <  1:8964583/101‑1 (MQ=255)
                                                                      tGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.tttc                                >  1:6311423/1‑99 (MQ=255)
                                                                      tGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTcc                                >  2:8010827/1‑101 (MQ=255)
                                                                      tGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTcc                                <  2:2118808/101‑1 (MQ=255)
                                                                        aTCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCCGTCGCTGACTCCGATGGGGCCGGC.TTCCgg                              >  1:7698419/1‑101 (MQ=255)
                                                                          cGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCGGgc                            >  2:6793940/1‑101 (MQ=255)
                                                                           gCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCcgggg                           >  1:3428683/1‑99 (MQ=255)
                                                                           gCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCGGgcg                           >  2:2420910/1‑101 (MQ=255)
                                                                            ccGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTTCGGgggc                          >  2:3272422/1‑101 (MQ=255)
                                                                             cGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGNGCCGGC.TTTCCGgcgcc                         >  2:3857731/1‑100 (MQ=255)
                                                                             cGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCGGGCGCt                         <  1:2420983/101‑1 (MQ=255)
                                                                             cGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCCGgggcc                         >  1:596078/1‑100 (MQ=255)
                                                                              gAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCCGGGGCTc                        >  2:1055152/1‑101 (MQ=255)
                                                                               aGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCCGCATCCGATGGGACCGGC.TTCCgcgggcccg                       >  1:3279770/1‑93 (MQ=255)
                                                                                 gACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCGGGCGCTCGAc                     <  1:1407351/101‑1 (MQ=255)
                                                                                  gcGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCGGGCGCTCGAcc                    <  2:5818718/100‑1 (MQ=255)
                                                                                   cGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCGGGCGCTCGACCg                   <  1:7560447/101‑1 (MQ=255)
                                                                                    gAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGCTTTCCggggcccgtcccg                   >  2:4664336/1‑91 (MQ=255)
                                                                                    gAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCGGGCGCTCGACCgg                  >  1:9140081/1‑101 (MQ=255)
                                                                                      ggTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCGGGCGCCCGCCCCGGc                >  2:1348842/1‑101 (MQ=255)
                                                                                           tCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCGGGCGCTCGGCCGGGCTgcgc           >  1:5711463/1‑101 (MQ=255)
                                                                                           tCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCGGGCGCTCGACCGGGCTgcgc           >  2:7526367/1‑101 (MQ=255)
                                                                                           tCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCGGGCGCCCGACCCGGCCgcgc           >  1:3957638/1‑101 (MQ=255)
                                                                                             tttCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCGGGCGCTCGACCGGGCTGCGCTg         <  1:5247298/101‑1 (MQ=255)
                                                                                               tCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCGGGCGCTCGACCGGGCTGCGCTGGc       <  1:5485883/101‑1 (MQ=255)
                                                                                                   gcCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCCGGGGCTCGACCCGGGTGCGCCGGCCGAt   >  2:2836384/1‑101 (MQ=255)
                                                                                                    cccccccccGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCGGGCGCTCGACCGGGCTGCGCTGGCCGATc  >  1:3588042/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
GGGCTGCGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCGATGGGACCGGC.TTCCGGGCGCTCGACCGGGCTGCGCTGGCCGATC  >  NC_014034/825938‑826138

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG