BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	904,926	Δ1 bp	97.9%	intergenic (+30/‑166)	RCAP_rcc00821 → / → murB	hypothetical protein/UDP‑N‑acetylmuramate dehydrogenase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	904,926	0	T→.	97.9%	14.6 ‑0.4 ‑0.4	292	intergenic (+30/‑166)	RCAP_rcc00821/murB	hypothetical protein/UDP‑N‑acetylmuramate dehydrogenase
Reads supporting (aligned to +/- strand): new base (137/149): ref base (2/4): total (139/153)
Fisher's exact test strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.30e-01

Only 100 of 341 total aligned reads displayed.


TGGCAGGCGGGCTGAGCGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCTTTTTG.G.CT..GC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGC  >  NC_014034/904826‑905020


 ggCAGGCGGGCTGAGCGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCtt                                                                                                     <  1:7701399/101‑1 (MQ=255)


   gaGGCGGGCTGGGGGTGGTTGGGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCttt                                                                                                    <  1:8938237/100‑1 (MQ=38)


   cAGGCGGGCTGAGCGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCtttt                                                                                                   <  2:9132881/101‑1 (MQ=255)


    aGGCGGGCTGAGCGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCttttg                                                                                                  <  2:5979476/101‑2 (MQ=255)


    aGGCGGGCTGAGCGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCttttg                                                                                                  >  1:8231729/1‑100 (MQ=255)


    aGGCGGGCTGAGCGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCttttg                                                                                                  >  1:5761463/1‑100 (MQ=255)


      gCGGGCTGAGCGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGGGCTGGCCCCCTCttttggc                                                                                                >  2:689851/1‑98 (MQ=255)


       ggggCGGAGCGT.GCGGGGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCttttggct                                                                                               <  1:2989471/100‑5 (MQ=38)


         ggCTGAGCGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gc                                                                                        >  1:2125362/1‑101 (MQ=255)


            tGAGCGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgcc                                                                                     <  2:3708319/101‑4 (MQ=255)


            tGAGCGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgcc                                                                                     >  1:9056544/1‑98 (MQ=255)


             gAGCGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCttttggcggcccgc                                                                                         >  1:7813994/1‑91 (MQ=255)


             gAGCGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgcca                                                                                    <  2:3301424/101‑5 (MQ=255)


             gAGCGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgcca                                                                                    <  1:1925195/101‑5 (MQ=255)


              aGCGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaa                                                                                   >  2:4767619/1‑96 (MQ=255)


              aGCGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaa                                                                                   >  2:5152428/1‑96 (MQ=255)


               gCGT.GCTGCGCTGGCCGGTGCGGCGGCTATTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaaa                                                                                  <  1:6404998/101‑7 (MQ=37)


                cGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaaaa                                                                                 <  1:6868646/101‑8 (MQ=37)


                cGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaaaa                                                                                 <  1:5526791/101‑8 (MQ=37)


                    gctgcGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgcccaaattc                                                                              >  2:1149862/1‑91 (MQ=37)


                      tgcGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCAc                                                                            <  2:4257613/101‑1 (MQ=25)


                       gcgcTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCAcc                                                                           <  1:5219382/101‑1 (MQ=25)


                        cgcTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CCAAATTCAccc                                                                          >  2:6754430/1‑101 (MQ=25)


                           tGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCTTTTTGCT.GC..GC..CAAAATTCAcccccc                                                                       >  1:1344007/1‑101 (MQ=25)


                           tGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCAcccccc                                                                       <  2:2425389/101‑1 (MQ=21)


                            ggCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CCAAATTCACCCCCCg                                                                      >  2:3869279/1‑101 (MQ=21)


                                ggTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCAACCCCCGGCGt                                                                  >  2:8807341/1‑101 (MQ=21)


                                  tGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTAt                                                                <  1:503341/101‑1 (MQ=21)


                                  tGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTAt                                                                >  2:1956973/1‑101 (MQ=21)


                                  tGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATCCACCCCCCGGCGTAt                                                                <  1:6743779/101‑1 (MQ=21)


                                     ggcgtcTCTTTGCCGCTATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTg                                                             <  2:1858757/101‑1 (MQ=18)


                                     ggcggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTg                                                             <  1:7607724/101‑1 (MQ=21)


                                      acggcTCTTTGCCGCCATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCTTTTTGCT.GC..GC..CAAAATTCACCCCCCGGCGTATTTGc                                                            <  1:2292136/100‑1 (MQ=21)


                                        ggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCAc                                                          <  2:8376505/101‑1 (MQ=21)


                                          ttctTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTTCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCa                                                        <  1:8717717/100‑1 (MQ=21)


                                          ctctTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCAcga                                                        >  2:1518947/1‑99 (MQ=18)


                                          ctctTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCa                                                        >  2:4606398/1‑101 (MQ=21)


                                             tttGCCGCGCTGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGa                                                     <  2:898862/101‑1 (MQ=18)


                                               tGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGaaa                                                   >  1:310510/1‑101 (MQ=21)


                                               tGCAGCGAGGCGCCGGGGCGAATGAGAGTGGAGCAGGAGTGCGCTGCCCCCCTC.TTTTG.G.CT..GCGGGAAAATTCGCCCCCCGGCGTATTTGCACCAGGaaa                                                   <  2:7987612/101‑1 (MQ=12)


                                                gCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGaaaa                                                  >  1:6228881/1‑101 (MQ=21)


                                                 cctgcaTGCCCCGGCGGTGCTGAGGGCGGGGCATGGGCGCTCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTTCCCCCCCGGCGTATTTGCACCAGGaaaaa                                                 <  1:4244462/96‑1 (MQ=9)


                                                      aTGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCg                                            >  1:6021192/1‑101 (MQ=21)


                                                      aTGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCg                                            >  2:2216099/1‑101 (MQ=21)


                                                       tGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCgg                                           <  2:388182/101‑1 (MQ=21)


                                                        gCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGc                                          >  2:7600760/1‑101 (MQ=21)


                                                          aCCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGt                                        >  2:5090666/1‑101 (MQ=21)


                                                           ccGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGtt                                       <  2:907689/101‑1 (MQ=21)


                                                            cGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGttt                                      >  2:1453528/1‑101 (MQ=21)


                                                             ggCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGtttt                                     >  2:6589686/1‑101 (MQ=21)


                                                             ggCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGtttt                                     >  1:3194964/1‑101 (MQ=21)


                                                             ggCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGCCNNATNTGCACCAGGAAAAAGCCCGGCGtttt                                     >  1:4558149/1‑101 (MQ=18)


                                                              gcgcGACTGAGGGCGGGGCAGGGGGGCGCTTCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGttttt                                    <  1:1084434/101‑1 (MQ=21)


                                                               cgcgACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTc                                   <  1:3780138/101‑1 (MQ=21)


                                                                gcgACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTcc                                  >  1:7257145/1‑101 (MQ=21)


                                                                gcgACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTcc                                  >  2:2777989/1‑101 (MQ=21)


                                                                 cgACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCt                                 >  1:2619782/1‑101 (MQ=21)


                                                                  gACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTg                                >  2:5504894/1‑101 (MQ=21)


                                                                  gACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTg                                >  1:162034/1‑101 (MQ=21)


                                                                  gACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCACGGCGTTTTTCCTg                                >  2:3191588/1‑101 (MQ=18)


                                                                   aCTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTgg                               >  1:2802187/1‑101 (MQ=21)


                                                                   aCTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTgg                               >  2:6318769/1‑101 (MQ=21)


                                                                    cTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGt                              <  1:3367790/101‑1 (MQ=21)


                                                                     tGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCTTTTTTCCTGGTc                             <  1:8194482/101‑1 (MQ=18)


                                                                     tGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTc                             <  2:6430685/101‑1 (MQ=21)


                                                                     tGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTc                             >  2:7398704/1‑101 (MQ=21)


                                                                      gAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCg                            >  2:2575940/1‑101 (MQ=21)


                                                                           cGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATa                       <  1:221528/101‑1 (MQ=21)


                                                                           cGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATa                       >  1:8504467/1‑101 (MQ=21)


                                                                           cGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATa                       >  1:537609/1‑101 (MQ=21)


                                                                           cGGGGCAGGGGGGCCCTGCGCCCCTCTTTTGG.GTGC..GC..CAAAACTCACCCCCCGGCGTATTTGCCCCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATa                       <  1:4129557/101‑1 (MQ=37)


                                                                             gggCAGGGGGGCGCTCCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCCCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACg                     <  1:4369771/101‑1 (MQ=21)


                                                                               gCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGcc                   >  2:5434333/1‑101 (MQ=21)


                                                                               gCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCAGGCGTATTGGCACCAGGAAAAAGCCCGGCGGTTCTACTGGTCGACATAGGcc                   >  2:8579417/1‑101 (MQ=17)


                                                                                cAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCg                  >  1:8056463/1‑101 (MQ=21)


                                                                                 aGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgg                 >  1:3663202/1‑101 (MQ=25)


                                                                                  ggggggCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCAGCCCCCGGCGTATTTGCACCCGGAAAAAGCCCGGCGTTTTTCCTGGTTGAAATACGCCggc                >  1:1670678/1‑100 (MQ=21)


                                                                                  ggggggCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCggg                >  1:5735476/1‑101 (MQ=25)


                                                                                   gggggCGCTGCCCCCCTCTTTTGG.G.TGC.GC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgggg               >  2:4387889/1‑101 (MQ=25)


                                                                                   gggggCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgggg               >  2:6982312/1‑101 (MQ=25)


                                                                                     gggCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgggggg             <  1:1957033/101‑1 (MQ=25)


                                                                                      ggCGCTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGa            <  1:6589946/101‑1 (MQ=25)


                                                                                         gcTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCAGCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTc         >  1:4773161/1‑101 (MQ=37)


                                                                                         gcTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTc         <  1:7156095/101‑1 (MQ=37)


                                                                                         gcTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTc         <  2:764980/101‑1 (MQ=37)


                                                                                          cTGCCCCCCTCTTTTGGTT.GC..GC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc        <  1:3907704/101‑1 (MQ=25)


                                                                                          cTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc        >  2:3011233/1‑101 (MQ=37)


                                                                                          cTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc        >  2:2000619/1‑101 (MQ=37)


                                                                                          cTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc        <  2:3061526/101‑1 (MQ=37)


                                                                                          cTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc        <  2:8504087/101‑1 (MQ=37)


                                                                                          cTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc        <  2:6151758/101‑1 (MQ=37)


                                                                                          cTGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc        <  1:3140487/101‑1 (MQ=37)


                                                                                          cTGCCCCCCGC.TTTTG.G.CTGCGC..CAAAATTCCCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc        <  2:1670631/101‑1 (MQ=25)


                                                                                           tGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCg       >  2:7184769/1‑101 (MQ=37)


                                                                                           tGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCg       <  2:8231379/101‑1 (MQ=37)


                                                                                           tGCCCCCCTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCg       <  2:4076661/101‑1 (MQ=37)


                                                                                             ccccccTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGCGTCCCCt     >  1:40358/1‑101 (MQ=25)


                                                                                             ccccccTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCt     <  1:7512755/101‑1 (MQ=37)


                                                                                               ccccTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTg   <  1:1126210/101‑1 (MQ=37)


                                                                                               ccccTC.TTTTG.G.CTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTg   <  2:5761230/101‑1 (MQ=37)


TGGCAGGCGGGCTGAGCGT.GCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCTTTTTG.G.CT..GC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGC  >  NC_014034/904826‑905020

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 39 ≤


ATCG