Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 985,690 Δ1 bp 100% intergenic (+29/‑55) betB → / → betA betaine‑aldehyde dehydrogenase/choline dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034985,6900C→.100.0% 134.3 385intergenic (+29/‑55)betB/betAbetaine‑aldehyde dehydrogenase/choline dehydrogenase
Reads supporting (aligned to +/- strand):  new base (119/217):  ref base (34/3):  total (165/220)
Fisher's exact test strand distribution p-value = 1.37e-11
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.61e-23

Only 100 of 452 total aligned reads displayed.

GCCGCGATCGAGCACTATACGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGCCGGTCCCCCCCCGGACCCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATCGTTGGGGCAGGCTCGGGCGGC  >  NC_014034/985590‑985786
                                                                                                    |                                                                                                
     gATCGAGCACTATACGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACNCAGGCCGGGGGGGCCCCCCCCCGCcccccc                                                                                             >  1:1976352/1‑101 (MQ=255)
     gATCGAGCACTATACGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGAcccccc                                                                                             <  2:4482688/101‑1 (MQ=255)
      tctgAGCACTATACGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGAccccccg                                                                                            <  2:5546265/98‑2 (MQ=255)
      aTCGAGCACTATACGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGGCCCCCCCCCGGccccccg                                                                                            >  2:7603229/1‑100 (MQ=255)
        cGAGCACTATACGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCCGACCCCCCCgc                                                                                          >  1:6908638/1‑100 (MQ=255)
         gAGCACTATACGCAGGTGAAGGCCGTCTATGTCGGCATGGGGTCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGAccccccggcg                                                                                         <  2:1936003/101‑5 (MQ=255)
          aGCACTATACGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGt                                                                                       <  2:2238233/101‑1 (MQ=255)
           gCACTATGCGCAGGGGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTa                                                                                      <  1:4273669/101‑1 (MQ=255)
           gCACTATACGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGGCCCCCCCCGG.GCCCCCCGGCGtg                                                                                      >  2:8244123/1‑100 (MQ=255)
             aCTATACGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTAtt                                                                                    <  1:506234/101‑1 (MQ=255)
             aCTATACGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTAtt                                                                                    <  1:5784413/101‑1 (MQ=255)
             aCTATACGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTAtt                                                                                    <  1:9064248/101‑1 (MQ=255)
             aCTATACGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGGCCCCCCCCGG.GCCCCCCGGCGGAtt                                                                                    >  2:7788542/1‑101 (MQ=255)
              cTATACGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCCG.GCCCCCCGGCGGAttt                                                                                   >  1:8631513/1‑101 (MQ=255)
              cTATACGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGGCCCCCCCCGGA.CCCCCCGGCGTGttt                                                                                   >  2:4826311/1‑101 (MQ=255)
               tataCGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTg                                                                                  <  1:3851007/101‑1 (MQ=255)
                ataCGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGGCCCCCCCCCGACCCCCCCgcggattttc                                                                                  >  1:4539806/1‑92 (MQ=255)
                  aCGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGGCCCCCCCCCG.GCCCCCCGGCGGATTTgcgc                                                                               >  2:6796185/1‑101 (MQ=255)
                    gCAGGTGAAGGCTGTCTATGTCGGCATGGGTCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCa                                                                             <  1:441713/101‑1 (MQ=255)
                    gCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCa                                                                             <  1:8072373/101‑1 (MQ=255)
                    gCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCa                                                                             <  1:4741366/101‑1 (MQ=255)
                    gCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGGCCCCCCCCGGACCCCCCC.GCGGATTTGCGCCa                                                                             >  2:5275183/1‑101 (MQ=255)
                      aGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGGCCCCCCCCGGA.CCCCCCGGCGGATTTTCGCCAAg                                                                           >  1:3317812/1‑101 (MQ=255)
                       ggTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGGATTTGCGCCAAGa                                                                          >  2:7402304/1‑101 (MQ=255)
                       ggTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGGCCCCCCCCGGA.CCCCCCGGCGGATTTGCGCCAAGa                                                                          >  1:8253311/1‑101 (MQ=255)
                        gTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGaa                                                                         <  1:5287096/101‑1 (MQ=255)
                          gAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGaaaa                                                                       <  2:269178/101‑1 (MQ=255)
                          gAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGaaaa                                                                       <  2:6160243/101‑1 (MQ=255)
                             ggCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGGCCCCCCCCGGACCCCCCC.GCGTATTTGCGCCAAGaaaaaaa                                                                    >  1:5446211/1‑100 (MQ=255)
                              gCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACa                                                                   <  1:2380557/101‑1 (MQ=255)
                               ccGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAg                                                                  <  2:3804810/101‑1 (MQ=255)
                                 gTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGACCCCCCC.GCGTATTTGCGCCAAGAAAAAACAggg                                                                >  2:8805534/1‑101 (MQ=255)
                                 gTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGGCCCCCCCCCGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAggg                                                                >  1:3129421/1‑101 (MQ=38)
                                   cTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAggggt                                                              >  2:5086066/1‑99 (MQ=37)
                                   cTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGtt                                                              <  1:324822/101‑1 (MQ=255)
                                    tATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTc                                                             <  1:9094977/101‑1 (MQ=255)
                                    tATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGGTc                                                             >  2:1405253/1‑101 (MQ=38)
                                    tATGTCGGCAGGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTc                                                             <  1:6536168/101‑1 (MQ=37)
                                     ttGTCGGCCTGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGGCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCa                                                            <  2:788768/100‑1 (MQ=38)
                                     aTGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCa                                                            <  1:6577726/101‑1 (MQ=255)
                                     aTGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGGCCCCCCCCGGACCCCCCC.GCGTATTTGCGCCAAGAAAAAACAGGGGTCa                                                            >  2:3166905/1‑101 (MQ=37)
                                      tGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAg                                                           <  1:895979/101‑1 (MQ=255)
                                      tGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAg                                                           <  2:661949/101‑1 (MQ=255)
                                      tGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAg                                                           <  1:3411026/101‑1 (MQ=255)
                                      tGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAg                                                           <  1:8408933/101‑1 (MQ=255)
                                       gTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCCGGCCGGGGGGGGCCCCCCCGCA.CCCCCAGCGGAATTTGCGCCAAGCACACACAGGGTCCacg                                                          >  2:2953194/1‑99 (MQ=255)
                                       gTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAgg                                                          <  2:2114007/101‑1 (MQ=255)
                                       gTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCCCCAAGAAAAAACAGGGTTCAgg                                                          >  2:5721619/1‑101 (MQ=38)
                                          ggCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCag                                                       <  1:6827378/101‑1 (MQ=255)
                                          ggCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCag                                                       <  2:363189/101‑1 (MQ=255)
                                          ggCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGGTCAGGCag                                                       >  1:2574470/1‑101 (MQ=38)
                                           gCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCaga                                                      <  2:3474236/101‑1 (MQ=255)
                                           gCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCaga                                                      <  2:4181304/101‑1 (MQ=255)
                                            cATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGGATTTGCGCCAAGAAAAAACAGGGGTCAGGCagag                                                     >  2:4682637/1‑101 (MQ=255)
                                             aTGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGACCCCCCC.GCGTATTTGCGCCCAGAAAAAACAGGGGTCAAGccgcgg                                                    >  1:5928128/1‑96 (MQ=255)
                                             aTGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCCGACCCCCCC.GCGGATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAgg                                                    >  1:1584069/1‑101 (MQ=255)
                                             aTGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGGCCCCCCCCCG.GCCCCCCGGGGGCTTTGCGCCCAGAAAAAAAAGGGGTCAGGCAGAgg                                                    >  1:7613181/1‑101 (MQ=255)
                                              tGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAggg                                                   <  2:5414968/101‑1 (MQ=255)
                                                gggCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTc                                                 <  1:739848/101‑1 (MQ=255)
                                                ggcccGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTc                                                 <  1:2942504/98‑1 (MQ=37)
                                                    cGGTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTcgcgc                                             <  1:1089173/101‑1 (MQ=255)
                                                     ggTCGAGAGCCCCTATTGAAACGCAGGGGGGGGGGGCGCCCCCCGGA.CCCCCCGGCGTATTTGCGGCAAGGAAAAACGAGGCGCAGGAGGATGGTtgcgcg                                            >  2:4286878/1‑101 (MQ=12)
                                                     ggTCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGGCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGGTCAGGCAGAGGGGcgcgcg                                            >  1:2456536/1‑101 (MQ=25)
                                                       tCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCgat                                          <  1:4863511/101‑1 (MQ=255)
                                                       tCGAGAGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAAAAGGGTTCAGGCAGAGGGGCGCGCgat                                          >  1:1358179/1‑101 (MQ=37)
                                                         gagaGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCgatgg                                        <  1:562581/101‑1 (MQ=255)
                                                         gagaGCCCCTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCgatgg                                        <  1:360526/101‑1 (MQ=255)
                                                                ccTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGa                                 <  2:8886891/101‑1 (MQ=255)
                                                                ccTATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGGTCAGGCAGAGGGGCGCGCGATGGATGCGGa                                 >  1:8736501/1‑101 (MQ=37)
                                                                  tATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGAtt                               <  2:4011360/101‑1 (MQ=255)
                                                                  tATTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGAtt                               <  2:8093432/101‑1 (MQ=255)
                                                                  tATTGAAACGCAGGCCGGGGGGTCCCCCCCCGG.GCCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGGTCAGGCAGAGGGGCGCGCGATGGATGCGGGtt                               >  1:8201149/1‑101 (MQ=37)
                                                                  tATTGAAACGCAGGCCGGGGGGCCCCCCCCCGG.GCCCCCCGGCGCCTTTTCGCCACCAAAAAACAGGGGTCTGGCTGGGGGACCCGCGGACGATGCGGGtt                               >  1:7931867/1‑101 (MQ=255)
                                                                   aTTGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGGTCAGGCCGAGGGGCGCGCGAGGGATGCGGGTTa                              >  1:7044963/1‑101 (MQ=25)
                                                                    ttGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTAt                             <  1:2132457/101‑1 (MQ=255)
                                                                    ttGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTAt                             <  1:1405285/101‑1 (MQ=255)
                                                                     tGAAACGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTAtg                            <  2:3300972/101‑1 (MQ=255)
                                                                     tGAAACGCAGGCCGGGGGGGCCCCCCCCCGACCCCCCC.GCGGATTTGCGCCAAGAAAAAAAAGGGGTCAGGCAGAGGGGCGCGCGATGGATGCGGATTAtg                            >  2:1746645/1‑101 (MQ=255)
                                                                         aCGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGAt                        <  1:4046451/101‑1 (MQ=255)
                                                                         aCGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGAt                        <  1:1188275/101‑1 (MQ=255)
                                                                          cGCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGGTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATc                       >  1:6292826/1‑101 (MQ=37)
                                                                           gCAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATCg                      <  1:1727108/101‑1 (MQ=255)
                                                                            cAGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATCGt                     >  2:8479681/1‑101 (MQ=255)
                                                                             aGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATCGtt                    <  1:2768754/101‑1 (MQ=255)
                                                                             aGGCCGGGGGGTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATCGtt                    >  2:2800117/1‑101 (MQ=255)
                                                                                ccgggggggccccccccCGGCCCCCCC.GCGGATTTTCGCCAAGAAAAAACAGGGGTCCGGCAGAGGGGCGCGCGGTGGGGGCGGGATTTGTGATTGTTtgg                 >  2:3359527/10‑101 (MQ=255)
                                                                                   gggggTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATCGTTGGGGCa              >  1:348441/4‑101 (MQ=255)
                                                                                    ggggTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATCGTTGGGGCAg             <  1:1509161/99‑1 (MQ=255)
                                                                                    ggggTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGGTCAGGCAGAGGGGCGCGCGATGGATGCGGATTATGTGATCGTTGGGGCAg             >  1:2314224/3‑101 (MQ=38)
                                                                                     gggTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTTTGTGATCGTTGGGGCAgg            <  1:9177626/100‑1 (MQ=255)
                                                                                       gTCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATCGTTGGGGCAGGCt          >  1:1889597/1‑101 (MQ=255)
                                                                                        tCCCCCCCCGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATCGTTGGGGCAGGCTc         <  1:7767559/101‑1 (MQ=255)
                                                                                        gccccccccGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATCGTTGGGGCAGGCTc         <  2:4340482/100‑1 (MQ=255)
                                                                                         ccccccccGGACCCCCCC.GCGTATTTGCGCCAAGAAAAAAAAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTTTGTGATCGTTTGGGGAGGCTCg        >  2:5650799/1‑101 (MQ=255)
                                                                                         ccccccccGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATCGTTGGGGCAGGCTCg        >  1:6189176/1‑101 (MQ=255)
                                                                                         ccccccccGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGGCGCGCGATGGATGCGGATTATGTGATCGTTGGGGCAGGCTCg        >  2:3733443/1‑101 (MQ=255)
                                                                                          cccccccGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATCGTTGGGGCAGGCTCgg       <  2:2479590/101‑1 (MQ=255)
                                                                                           ccccccGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATCGTTGGGGCAGGCTCggg      <  2:6974788/101‑1 (MQ=255)
                                                                                              cccGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATCGTTGGGGCAGGCTCGggcgg   >  2:711640/1‑101 (MQ=255)
                                                                                               ccGGA.CCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATCGTTGGGGCAGGCTCGgggggc  >  2:1198080/1‑101 (MQ=255)
                                                                                                    |                                                                                                
GCCGCGATCGAGCACTATACGCAGGTGAAGGCCGTCTATGTCGGCATGGGGCCGGTCGAGAGCCCCTATTGAAACGCAGGCCGGCCGGTCCCCCCCCGGACCCCCCCGGCGTATTTGCGCCAAGAAAAAACAGGGTTCAGGCAGAGGGTCGCGCGATGGATGCGGATTATGTGATCGTTGGGGCAGGCTCGGGCGGC  >  NC_014034/985590‑985786

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG