Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014035 34,742 Δ1 bp 3.7% coding (400/1041 nt) RCAP_rcp00049 ← iron siderophore/cobalamin ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_01403534,7420T→. 3.7% 7.4 ‑3.6 0.0136coding (400/1041 nt)RCAP_rcp00049iron siderophore/cobalamin ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (0/5):  ref base (69/62):  total (69/67)
Fisher's exact test strand distribution p-value = 2.68e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

GCGACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATGGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTCG  >  NC_014035/34642‑34836
                                                                                                    |                                                                                              
 cggcccccGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATg                                                                                               <  2:7918432/98‑1 (MQ=17)
 cgACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATg                                                                                               >  1:3365730/1‑101 (MQ=32)
 cgACCCCCGCGAGGATCGTGTGGTTCGGCCCGCCGCCCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCCAAAGCCCCCATg                                                                                               >  1:4204362/1‑101 (MQ=11)
  gACCCCCGCGAGTATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATgg                                                                                              <  1:1993361/101‑1 (MQ=17)
  gACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATgg                                                                                              <  1:9067428/101‑1 (MQ=32)
  gACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATgg                                                                                              >  1:6276337/1‑101 (MQ=32)
  gACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATgg                                                                                              >  2:6309558/1‑101 (MQ=32)
   aCCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATgga                                                                                             >  2:2157862/1‑100 (MQ=14)
   aCCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATgga                                                                                             >  2:1975236/1‑100 (MQ=14)
   aCCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATgga                                                                                             <  2:1198541/101‑2 (MQ=14)
    cccccGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATggac                                                                                            <  2:1841068/101‑3 (MQ=11)
    cccccGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATggac                                                                                            <  1:6994217/101‑3 (MQ=11)
                       gatCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGcc                                                                        >  2:8960115/3‑101 (MQ=2)
                        ttCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCg                                                                       <  2:5533745/101‑1 (MQ=14)
                         tCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGa                                                                      <  1:6309798/101‑1 (MQ=17)
                         tCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGa                                                                      >  1:1405603/1‑101 (MQ=17)
                           ggCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATg                                                                    <  2:5713656/101‑1 (MQ=17)
                           ggCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATg                                                                    >  2:77811/1‑101 (MQ=17)
                           ggCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATg                                                                    >  1:1100898/1‑101 (MQ=17)
                            gCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGc                                                                   <  1:3155486/101‑1 (MQ=17)
                              ccGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGccc                                                                 <  1:931162/101‑1 (MQ=17)
                              ccGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGccc                                                                 <  1:5105169/101‑1 (MQ=17)
                              ccGCTCGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGccc                                                                 <  2:3724882/101‑1 (MQ=12)
                               cGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCa                                                                <  2:357247/101‑1 (MQ=17)
                               cGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCa                                                                <  1:4773589/101‑1 (MQ=17)
                                gCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAg                                                               >  2:1147279/1‑101 (MQ=17)
                                 cTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGCTGCCCAGc                                                              >  1:2897568/1‑101 (MQ=12)
                                 cTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGc                                                              <  1:3759485/101‑1 (MQ=17)
                                 cTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGc                                                              <  2:5957416/101‑1 (MQ=17)
                                 cTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGc                                                              <  1:2157924/101‑1 (MQ=17)
                                   ggTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCAc                                                            <  1:7888191/101‑1 (MQ=17)
                                   ggTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCAc                                                            <  2:8870446/101‑1 (MQ=17)
                                   ggTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCAc                                                            >  1:5324325/1‑101 (MQ=17)
                                   ggTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGACCGCCCGGCCGATGCCCAGCAc                                                            >  2:3392759/1‑101 (MQ=12)
                                    gTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACg                                                           >  1:258116/1‑101 (MQ=17)
                                      cgcgCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGATGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGAt                                                         >  2:7015959/1‑101 (MQ=12)
                                      cgcgCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGAt                                                         >  1:5876647/1‑101 (MQ=17)
                                      cgcgCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGAt                                                         >  2:7396804/1‑101 (MQ=17)
                                       gcgcCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATg                                                        <  1:5856819/101‑1 (MQ=17)
                                       gcgcCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATg                                                        <  1:666457/101‑1 (MQ=17)
                                        cgcCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGa                                                       <  2:4829997/101‑1 (MQ=17)
                                         gcCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGAc                                                      <  1:3287056/101‑1 (MQ=17)
                                          ccGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGAcc                                                     <  2:9107385/101‑1 (MQ=17)
                                          ccGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGAcc                                                     >  1:3920768/1‑101 (MQ=18)
                                           cGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCg                                                    >  1:4300897/1‑101 (MQ=18)
                                            gTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGc                                                   >  1:2104417/1‑101 (MQ=18)
                                            gTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGc                                                   >  2:711055/1‑101 (MQ=18)
                                            gTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGc                                                   >  2:9026377/1‑101 (MQ=18)
                                             ttCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGcc                                                  <  1:2865089/101‑1 (MQ=17)
                                             ttCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGcc                                                  >  1:6933707/1‑101 (MQ=18)
                                              tCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCa                                                 <  1:3343232/101‑1 (MQ=17)
                                              tCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCa                                                 >  1:7127352/1‑101 (MQ=17)
                                               cGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAc                                                <  2:455811/101‑1 (MQ=17)
                                               cGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAc                                                <  2:5324141/101‑1 (MQ=17)
                                               cGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAc                                                >  2:6901517/1‑101 (MQ=18)
                                                gACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAcc                                               <  2:2836529/101‑1 (MQ=17)
                                                gACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAcc                                               <  2:9052599/101‑1 (MQ=17)
                                                 aCAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcg                                              >  2:5522022/1‑101 (MQ=18)
                                                 aCAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcg                                              >  1:3569438/1‑101 (MQ=18)
                                                 aCAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcg                                              >  2:4684049/1‑101 (MQ=18)
                                                 aCAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcg                                              <  1:4258872/101‑1 (MQ=17)
                                                  cAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcgc                                             >  2:2424134/1‑101 (MQ=18)
                                                  cAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcgc                                             >  2:5276202/1‑101 (MQ=18)
                                                  cAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcgc                                             <  1:4107273/101‑1 (MQ=17)
                                                   aaaaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcgcg                                            >  1:3602538/1‑101 (MQ=17)
                                                    aaaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCgcgc                                           >  1:7360776/1‑101 (MQ=18)
                                                      aGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCg                                         <  1:1601883/101‑1 (MQ=17)
                                                        cgcCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGt                                       >  2:3065490/1‑101 (MQ=18)
                                                         gcCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTc                                      >  2:7760485/1‑101 (MQ=18)
                                                         gcCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTc                                      >  1:5346607/1‑101 (MQ=17)
                                                          ccaccaGCGCGAAGGCAGCGAAGGCGCCTGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCg                                     <  1:6400311/101‑1 (MQ=12)
                                                          ccaccaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCg                                     <  1:4858644/101‑1 (MQ=17)
                                                          ccaccaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCg                                     >  2:3604969/1‑101 (MQ=18)
                                                           caccaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGa                                    >  2:4353745/1‑101 (MQ=18)
                                                            accaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAg                                   >  1:3659764/1‑101 (MQ=18)
                                                            accaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAg                                   >  2:3161654/1‑101 (MQ=18)
                                                            accaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAg                                   >  2:3153907/1‑101 (MQ=18)
                                                             ccaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAgg                                  <  1:149084/101‑1 (MQ=17)
                                                              caGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCCCCGCGCCGGTCGCGGc                                 >  1:1411723/1‑101 (MQ=255)
                                                              caGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGc                                 >  1:6253135/1‑101 (MQ=17)
                                                              caGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGc                                 >  2:6142402/1‑101 (MQ=17)
                                                              caGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGc                                 >  2:1785832/1‑101 (MQ=17)
                                                               aGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGcc                                <  2:473205/101‑1 (MQ=17)
                                                               aGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGcc                                >  1:2700784/1‑101 (MQ=17)
                                                                  gcgAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGccccc                             <  2:6510082/101‑1 (MQ=17)
                                                                  gcgAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGccccc                             <  2:1122335/101‑1 (MQ=17)
                                                                  gcgAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGccccc                             <  2:8668790/101‑1 (MQ=17)
                                                                  gcgAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGccccc                             <  2:3783919/101‑1 (MQ=17)
                                                                   cgAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCccg                            >  2:546737/1‑101 (MQ=17)
                                                                   cgAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCccg                            <  2:8282960/101‑1 (MQ=17)
                                                                     aaGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCccgcc                          >  1:5730024/1‑101 (MQ=17)
                                                                      aGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCccgccg                         <  1:2993900/101‑1 (MQ=17)
                                                                       ggCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCga                        >  1:8525449/1‑101 (MQ=17)
                                                                       ggCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCga                        <  2:2421003/101‑1 (MQ=17)
                                                                       ggCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCga                        >  2:5480757/1‑101 (MQ=17)
                                                                        gCAGGGAAGGCGCCCGCGAAAG.CCCCNNNNNACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCgag                       <  1:5542954/101‑1 (MQ=11)
                                                                         cAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCgaga                      >  2:8074099/1‑101 (MQ=14)
                                                                          aGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGAc                     >  1:7790737/1‑101 (MQ=11)
                                                                                       gcgAAAGCCCCCA.GGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAg        <  2:258113/101‑1 (MQ=17)
                                                                                             gCCCCCA.GGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTCg  <  2:6705235/101‑1 (MQ=17)
                                                                                                    |                                                                                              
GCGACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATGGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTCG  >  NC_014035/34642‑34836

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG