BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,200,794	+G	100%	intergenic (+3/‑114)	trpF → / → trpB1	phosphoribosylanthranilate isomerase/tryptophan synthase subunit beta

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,200,794	1	.→G	100.0%	235.6	450	intergenic (+3/‑114)	trpF/trpB1	phosphoribosylanthranilate isomerase/tryptophan synthase subunit beta
Reads supporting (aligned to +/- strand): new base (107/268): ref base (52/23): total (159/291)
Fisher's exact test strand distribution p-value = 4.99e-11
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.62e-14

Only 100 of 518 total aligned reads displayed.


GGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGT.GGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGAA  >  NC_014034/1200694‑1200890


gggCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGt                                                                                                   <  1:4687369/101‑1 (MQ=255)


    ggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGT.gggg                                                                                              <  2:5240419/101‑1 (MQ=255)


     gcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGT.ggggg                                                                                             <  2:2579397/101‑2 (MQ=255)


     gcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGT.ggggg                                                                                             >  2:1163931/1‑100 (MQ=255)


      cTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGT.gggggg                                                                                            >  2:6563336/1‑99 (MQ=255)


       tCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGT.GGGGgtt                                                                                           <  2:227860/101‑1 (MQ=255)


       tCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGT.GGGGgtt                                                                                           <  1:9119700/101‑1 (MQ=255)


        cAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGT.ggggggtt                                                                                          >  2:3706412/1‑97 (MQ=255)


         aGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGG.ggggggttc                                                                                         >  1:7540838/1‑96 (MQ=255)


          ggTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTca                                                                                         <  2:2602800/101‑1 (MQ=255)


            tCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTcacc                                                                                       <  1:638170/101‑1 (MQ=255)


            tCGACGTGTCGTCGGGGGTGCAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTcacc                                                                                       <  1:7208385/101‑1 (MQ=255)


              gACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TAGGGGG.GGGGGTTcacccc                                                                                     >  1:3716139/1‑101 (MQ=255)


              gACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGGTTcacccc                                                                                     >  1:7006363/1‑101 (MQ=255)


              gACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.GA.GGGT.ggggggttcgcccc                                                                                    >  2:7139491/1‑91 (MQ=255)


                cGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTcaccccca                                                                                   <  1:5180005/101‑1 (MQ=255)


                cGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTcaccccca                                                                                   <  1:4085385/101‑1 (MQ=255)


                   gtcgtcGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTcacccccacac                                                                                <  2:8039204/101‑1 (MQ=255)


                   gtcgtcGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTcacccccacac                                                                                <  2:3395659/101‑1 (MQ=255)


                     cgtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTcacccccacaccc                                                                              <  1:998291/101‑1 (MQ=255)


                     cgtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTcacccccacaccc                                                                              <  1:7905028/101‑1 (MQ=255)


                       tcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TAGGGGG.GGGGTTTcacccccaccccccc                                                                            >  2:5163148/1‑101 (MQ=255)


                       tcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTcacccccacaccccc                                                                            <  2:869227/101‑1 (MQ=255)


                       tcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTcacccccacaccccc                                                                            <  1:307737/101‑1 (MQ=255)


                       tcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTcacccccacaccccc                                                                            <  2:7552985/101‑1 (MQ=255)


                       tcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTcacccccacaccccc                                                                            <  2:7278238/101‑1 (MQ=255)


                       gcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTcacccccacaccccc                                                                            <  2:7777385/100‑1 (MQ=255)


                        cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGTCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTcacccccacaccccca                                                                           <  2:463011/101‑1 (MQ=255)


                          ggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TAGGGGG.GGGGGTTCACCCCCCCACCCCCCGc                                                                         >  1:3230365/1‑101 (MQ=255)


                           gggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TAGGGGG.GGGGGTTCACCCCCACACCCCCAGCg                                                                        >  2:2124765/1‑101 (MQ=255)


                           gggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCg                                                                        <  1:66324/101‑1 (MQ=255)


                            ggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGt                                                                       <  2:3547170/101‑1 (MQ=255)


                             gTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTa                                                                      <  1:4243443/101‑1 (MQ=255)


                               ggAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGGTTCACCCCCACACCCCCCGccgctt                                                                    >  2:7024828/1‑96 (MQ=255)


                                gAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGGTTCACCCCCACACCCCCCGCGTAttt                                                                   >  2:64913/1‑101 (MQ=255)


                                 aaTCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TAGGGGG.GGGGTTTCACCCCCACACCCCCAGCGCAtttt                                                                  >  2:7422793/1‑101 (MQ=255)


                                  aTCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTg                                                                 <  1:894810/101‑1 (MQ=255)


                                   tCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTgg                                                                <  2:2718171/101‑1 (MQ=255)


                                    cGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTTGc                                                               >  2:1692078/1‑101 (MQ=255)


                                    cGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGc                                                               <  1:1044502/101‑1 (MQ=255)


                                     ggCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGcc                                                              <  2:8269875/101‑1 (MQ=255)


                                      gcgcCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCa                                                             <  2:838897/101‑1 (MQ=255)


                                       gggccGGGGGTAAGGGGGGCGCCCGGATTTGCGGCTTTGGCGGGGGCGGGCAGGGGTTG.GGGG.GGGGTTTCCCCCCCCCCCCCCCAGCGTATTTTGGCCaa                                                            <  1:1868260/98‑1 (MQ=255)


                                        gcCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCaag                                                           <  1:6711343/101‑1 (MQ=255)


                                         cccGGGGTCGAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCaaga                                                          >  2:568198/1‑101 (MQ=255)


                                         cccGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCaaga                                                          <  1:3947801/101‑1 (MQ=255)


                                           cGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCaagaag                                                        <  2:8500609/101‑1 (MQ=255)


                                           cGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATGTTGGCCaagaag                                                        <  1:6563596/101‑1 (MQ=255)


                                            ggggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCCCCCCCACACCCCCAGCGTATTTTGGCCAagaaga                                                       <  1:6177535/101‑1 (MQ=255)


                                             gggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAgaagaa                                                      <  1:7289838/101‑1 (MQ=255)


                                             gggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAgaagaa                                                      <  2:8912830/101‑1 (MQ=255)


                                             gggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTGGGCCAAgaagaa                                                      <  1:8192153/101‑1 (MQ=255)


                                               gTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAAc                                                    <  1:6050563/101‑1 (MQ=255)


                                               gTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.GAGGGGG.GGGGATTTACCCCCCCCCCCCCCCCGCATTTTGGGCAAGAAGAAAc                                                    >  1:169598/1‑101 (MQ=255)


                                                tCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TAGGGGG.GGGGGTTCACCCCCACACCCCCCGCGTATTTTGGCCCAGAAGGAACa                                                   >  2:39612/1‑101 (MQ=255)


                                                tCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TAGGGGG.GGGGGTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACa                                                   >  1:4571691/1‑101 (MQ=255)


                                                 cAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTTACCCCCACACCCCCAGCGTATTTTTGCCAAGAAAAAAAAg                                                  >  2:307902/1‑101 (MQ=255)


                                                 cAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAg                                                  <  1:3529733/101‑1 (MQ=255)


                                                 cAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGGTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAg                                                  >  2:1590529/1‑101 (MQ=255)


                                                  aaGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TAGGGGG.GGGGGTTCACCCCCCCCCCCCCAGCGGATTTTGGCCAAGAAGAAACAgg                                                 >  1:741888/1‑101 (MQ=255)


                                                  aaGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAgg                                                 <  1:3841867/101‑1 (MQ=255)


                                                   aggaggCCGCCGGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGa                                                <  2:436045/101‑1 (MQ=255)


                                                   aggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGa                                                <  1:5024394/101‑1 (MQ=255)


                                                     gaggCCTCCCGGATTGCCGCCTTTGTNNNNNCTGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAAc                                              <  1:5347258/101‑1 (MQ=255)


                                                     gaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAAc                                              <  1:1563690/101‑1 (MQ=255)


                                                      aggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACt                                             <  1:7648789/101‑1 (MQ=255)


                                                       ggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACtt                                            <  2:8154821/101‑1 (MQ=255)


                                                       ggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACtt                                            <  1:1136392/101‑1 (MQ=255)


                                                         ccgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTa                                          <  2:3448980/101‑1 (MQ=255)


                                                          cgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTAc                                         <  2:137165/101‑1 (MQ=255)


                                                           gccGGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCACGAAGAAACAGGAACTTTAcc                                        >  1:5622122/1‑101 (MQ=255)


                                                           gccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTAcc                                        <  1:2461776/101‑1 (MQ=255)


                                                            cccGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTAccc                                       <  1:261728/101‑1 (MQ=255)


                                                              cGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCg                                     <  1:4919322/101‑1 (MQ=255)


                                                              cGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCg                                     <  1:8585265/101‑1 (MQ=255)


                                                               ggATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGa                                    <  2:7864035/101‑1 (MQ=255)


                                                               ggATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGa                                    >  1:9021232/1‑101 (MQ=255)


                                                                gATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAg                                   <  2:2872098/101‑1 (MQ=255)


                                                                gATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGGTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAg                                   >  2:4176419/1‑101 (MQ=255)


                                                                 aTTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACAACCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAgg                                  >  2:683574/1‑101 (MQ=255)


                                                                       gccTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAgggggggg                            <  1:8692413/101‑1 (MQ=255)


                                                                       gccTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAgggggggg                            <  2:5455031/101‑1 (MQ=255)


                                                                        ccTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGgc                           <  2:4717167/101‑1 (MQ=255)


                                                                         cTTTGTCGCGGCGGCGCAGGGG.TAGGGGG.GGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGgcg                          >  2:5737443/1‑101 (MQ=255)


                                                                         cTTTGTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGgcg                          <  2:1849381/101‑1 (MQ=255)


                                                                             gTCGCGGCGGCGCAGGGG.TAGGGGG.GGGGGTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGgggcgcg                      >  1:130711/1‑100 (MQ=255)


                                                                             gTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCCCCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCt                      <  1:2994519/101‑1 (MQ=255)


                                                                             gTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCt                      >  2:492453/1‑101 (MQ=255)


                                                                             gTCGCGGCGGCGCAGGGG.TA.GGGTGGGGGGTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACACCGAGGGGGGGGCGCGCt                      >  1:8485765/1‑101 (MQ=255)


                                                                               cgcgGCGGCGCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTAt                    <  2:6724010/101‑1 (MQ=255)


                                                                                 cggcggcgCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATct                  <  2:9104745/101‑1 (MQ=255)


                                                                                  ggcggcgCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctg                 <  2:7953818/101‑1 (MQ=255)


                                                                                  ggcggcgCAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctg                 >  1:572531/1‑101 (MQ=255)


                                                                                      gcgcAGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGt             >  2:6283393/1‑101 (MQ=255)


                                                                                          aGGGG.TA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTNTNggg         >  1:2316875/1‑100 (MQ=255)


                                                                                            ggg.GA.GGGTGGGGGTATCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGgcgc       >  1:8827272/1‑101 (MQ=255)


                                                                                                tA.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTg    >  2:3748071/1‑101 (MQ=255)


                                                                                                 a.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATTTGCTGTTGGCCCGTGa   >  1:2045794/1‑101 (MQ=255)


                                                                                                 a.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGa   >  1:2199091/1‑101 (MQ=255)


                                                                                                 a.GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGa   >  2:6939791/1‑101 (MQ=255)


GGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGG.TA.GGGT.GGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGAA  >  NC_014034/1200694‑1200890

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG