Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,255,865 +GCCC 100% intergenic (+35/+207) RCAP_rcc01184 → / ← RCAP_rcc01185 CsbD family protein/methyl‑accepting chemotaxis sensory transducer

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,255,8651.→G100.0% 309.2 154intergenic (+35/+207)RCAP_rcc01184/RCAP_rcc01185CsbD family protein/methyl‑accepting chemotaxis sensory transducer
Reads supporting (aligned to +/- strand):  new base (90/64):  ref base (0/0):  total (90/64)
*NC_0140341,255,8652.→C100.0% 321.2 154intergenic (+35/+207)RCAP_rcc01184/RCAP_rcc01185CsbD family protein/methyl‑accepting chemotaxis sensory transducer
Reads supporting (aligned to +/- strand):  new base (89/64):  ref base (0/0):  total (90/64)
*NC_0140341,255,8653.→C100.0% 331.8 154intergenic (+35/+207)RCAP_rcc01184/RCAP_rcc01185CsbD family protein/methyl‑accepting chemotaxis sensory transducer
Reads supporting (aligned to +/- strand):  new base (90/64):  ref base (0/0):  total (90/64)
*NC_0140341,255,8654.→C100.0% 331.6 155intergenic (+35/+207)RCAP_rcc01184/RCAP_rcc01185CsbD family protein/methyl‑accepting chemotaxis sensory transducer
Reads supporting (aligned to +/- strand):  new base (90/65):  ref base (0/0):  total (90/65)

Only 100 of 193 total aligned reads displayed.

CAGAAATACGGCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGG....CTG.TCTCGGTCCGCCCGAAGAGGGGGGGCGCTGTGGTAC..CCCTGCGGGACTCCCCCCGGGATATTTCCGCCAA  >  NC_014034/1255765‑1255938
                                                                                                     ||||                                                                            
cagAAATACGGCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgg                                                                                  >  2:8798155/1‑101 (MQ=255)
cagAAATACGGCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgg                                                                                  <  1:3988761/101‑1 (MQ=255)
 agAAATACGGCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCggg                                                                                 <  2:6350979/101‑2 (MQ=255)
 agAAATACGGCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCggg                                                                                 >  2:315785/1‑100 (MQ=255)
  gAAATACGGCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgggc                                                                                >  1:5590496/1‑99 (MQ=255)
   aaaTACGGCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgggcc                                                                               >  1:4525539/1‑98 (MQ=255)
   aaaTACGGCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgggcc                                                                               >  1:6165955/1‑98 (MQ=255)
   aaaTACGGCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgggcc                                                                               >  2:3730306/1‑98 (MQ=255)
   aaaTACGGCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgggcc                                                                               >  2:4379176/1‑98 (MQ=255)
     aTACGGCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgggcccc                                                                             >  2:4493913/1‑96 (MQ=255)
      tACGGCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgggccccc                                                                            <  1:2486769/101‑7 (MQ=255)
      tACGGCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgggccccc                                                                            >  2:2435626/1‑95 (MQ=255)
       gcGGCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgggcccccg                                                                           <  2:1965478/100‑8 (MQ=255)
       aCGGCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgggcccccg                                                                           >  2:1730377/1‑94 (MQ=255)
         ggCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgggcccccgct                                                                         >  2:3485121/1‑92 (MQ=255)
         ggCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgggcccccgct                                                                         <  1:3746454/101‑10 (MQ=255)
         ggCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgggcccccgct                                                                         >  1:5713647/1‑92 (MQ=255)
          gCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgggcccccgctt                                                                        <  2:4981923/101‑11 (MQ=255)
          gCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCgggcccccgctt                                                                        <  2:5765413/101‑11 (MQ=255)
                              aagaagGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAgggg                                                    >  2:2719263/1‑101 (MQ=255)
                               agaagGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTGCGGGCCCCCGCTTTCGGTCCGCCCGAAGAggggg                                                   >  2:5906318/1‑101 (MQ=255)
                                gaaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAgggggg                                                  >  1:8530037/1‑101 (MQ=255)
                                gaaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAgggggg                                                  >  1:6138074/1‑101 (MQ=255)
                                gaaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAgggggg                                                  >  2:2525259/1‑101 (MQ=255)
                                gaaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAgggggg                                                  >  1:1892139/1‑101 (MQ=255)
                                 aaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAggggggg                                                 <  1:867396/101‑1 (MQ=255)
                                 aaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAggggggg                                                 >  2:3298425/1‑101 (MQ=255)
                                 aaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAggggggg                                                 >  2:2390383/1‑101 (MQ=255)
                                  aggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGgc                                                >  1:2612854/1‑101 (MQ=255)
                                  aggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGgc                                                >  1:2257966/1‑101 (MQ=255)
                                  aggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGgc                                                <  2:6200092/101‑1 (MQ=255)
                                  aggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGACCCCGCTTTCGGTCCGCCCGAAGAGGGGGGgc                                                >  2:8508733/1‑101 (MQ=255)
                                   ggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGgcg                                               <  1:5311798/101‑1 (MQ=255)
                                    gaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGgcgc                                              >  2:198727/1‑101 (MQ=255)
                                    gaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGgcgc                                              >  2:5178275/1‑101 (MQ=255)
                                    gaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGgcgc                                              >  2:4841397/1‑101 (MQ=255)
                                     aagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGCAGAGGGGGGGCGCt                                             >  2:6431635/1‑101 (MQ=255)
                                      agTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGgcgccg                                            >  1:6534541/1‑99 (MQ=255)
                                      agTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtg                                            <  2:7929634/101‑1 (MQ=255)
                                      agTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtg                                            >  1:2433856/1‑101 (MQ=255)
                                      agTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtg                                            >  1:8886240/1‑101 (MQ=255)
                                       gTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgc                                           <  1:9152178/101‑2 (MQ=255)
                                        tCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGCAGAGGGGGCGCGCtgcc                                          >  1:2788714/1‑99 (MQ=255)
                                         cgacgaCTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccc                                         <  2:8378296/101‑4 (MQ=255)
                                         cgacgaCTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccc                                         <  1:2390456/101‑4 (MQ=255)
                                          gacgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgcccc                                        >  1:1190155/1‑97 (MQ=255)
                                           acgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAggggggggggtgccccc                                       >  1:2741384/1‑91 (MQ=255)
                                           acgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccc                                       >  2:4676467/1‑96 (MQ=255)
                                           acgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccc                                       >  1:7276948/1‑96 (MQ=255)
                                           acgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccc                                       <  1:4741974/101‑6 (MQ=255)
                                            cgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgcccccc                                      <  1:3106758/101‑7 (MQ=255)
                                            cgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgcccccc                                      <  2:7769951/101‑7 (MQ=255)
                                             gacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccccc                                     >  1:4219/1‑94 (MQ=255)
                                              acTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgt                                    <  2:1041000/101‑9 (MQ=255)
                                              acTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgt                                    >  2:8237135/1‑93 (MQ=255)
                                               cTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgtc                                   >  2:4285714/1‑92 (MQ=255)
                                               cTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgtc                                   <  2:6377796/101‑10 (MQ=255)
                                                ttCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgtcc                                  >  2:4199642/1‑91 (MQ=255)
                                                ttCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgtcc                                  <  1:4338399/101‑11 (MQ=255)
                                                ttCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgtcc                                  >  2:7408636/1‑91 (MQ=255)
                                                               tGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTccccc                   >  1:758075/1‑101 (MQ=9)
                                                                gcccgaGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTcccccc                  <  2:8940327/96‑1 (MQ=2)
                                                                gACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTcccccc                  <  2:6943965/101‑1 (MQ=9)
                                                                gACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTcccccc                  <  1:5346351/101‑1 (MQ=9)
                                                                gACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTcccccc                  >  1:547328/1‑101 (MQ=9)
                                                                 aCCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCg                 <  2:3972107/101‑1 (MQ=9)
                                                                 aCCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCg                 >  1:7530587/1‑101 (MQ=9)
                                                                  ccTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCgg                >  1:7120785/1‑101 (MQ=2)
                                                                   cTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCggg               >  1:1154146/1‑101 (MQ=2)
                                                                   cTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCggg               >  2:1492546/1‑101 (MQ=2)
                                                                   cTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCggg               >  2:396322/1‑101 (MQ=2)
                                                                   cTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCggg               >  1:8782033/1‑101 (MQ=2)
                                                                   cTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCggg               >  1:6456892/1‑101 (MQ=2)
                                                                    tAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGa              >  1:1336300/1‑101 (MQ=2)
                                                                    tAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGa              >  2:8155869/1‑101 (MQ=2)
                                                                    tAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGa              >  1:3162362/1‑101 (MQ=2)
                                                                    tAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGa              >  2:2194686/1‑101 (MQ=2)
                                                                    tAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGa              >  2:1283351/1‑101 (MQ=2)
                                                                    tAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGa              >  1:9118625/1‑101 (MQ=2)
                                                                     aGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGat             <  1:1790427/101‑1 (MQ=2)
                                                                     aGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGat             <  2:2109370/101‑1 (MQ=2)
                                                                     aGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGat             >  1:4570310/1‑101 (MQ=2)
                                                                     aGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGat             >  1:7894162/1‑101 (MQ=2)
                                                                      ggggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGata            <  2:8586238/101‑1 (MQ=2)
                                                                      ggggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGata            >  2:1291988/1‑101 (MQ=2)
                                                                       gggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGatat           >  2:5617128/1‑101 (MQ=2)
                                                                       gggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGatat           >  1:8205170/1‑101 (MQ=2)
                                                                        ggggCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAtt          <  2:5402703/98‑1 (MQ=0)
                                                                        ggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAtt          <  1:4863490/101‑1 (MQ=2)
                                                                        ggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAtt          >  2:3113227/1‑101 (MQ=2)
                                                                        ggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAtt          <  1:1326589/101‑1 (MQ=2)
                                                                        ggTCCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAtt          <  1:5211890/101‑1 (MQ=0)
                                                                         gTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAttt         >  1:3316617/1‑101 (MQ=0)
                                                                         gTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAttt         >  1:3146065/1‑101 (MQ=0)
                                                                         gTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCATGGCTAttt         >  2:8168488/1‑101 (MQ=0)
                                                                           gCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTcc       >  1:6975867/1‑101 (MQ=2)
                                                                              ggaccggaACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGcc    >  2:6771365/1‑101 (MQ=2)
                                                                               gaccggaACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCa   >  2:4320609/1‑101 (MQ=2)
                                                                               gaccggaACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCa   <  1:8256188/101‑1 (MQ=2)
                                                                               gaccggaACCGGGGGCCTTCGGGCCCCCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCa   <  1:7670399/101‑1 (MQ=2)
                                                                                                     ||||                                                                            
CAGAAATACGGCCGCACCAAGGAGGCGGCGAAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGG....CTG.TCTCGGTCCGCCCGAAGAGGGGGGGCGCTGTGGTAC..CCCTGCGGGACTCCCCCCGGGATATTTCCGCCAA  >  NC_014034/1255765‑1255938

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG