Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,303,831 +T 98.7% intergenic (+18/+139) urtE → / ← RCAP_rcc01231 urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,303,8312.→T98.7% 6.9 ‑1.5 ‑0.8455intergenic (+18/+139)urtE/RCAP_rcc01231urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (172/275):  ref base (4/2):  total (177/278)
Fisher's exact test strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.42e-01

Only 100 of 512 total aligned reads displayed.

GCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCT.C..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGCC  >  NC_014034/1303732‑1303921
                                                                                                        |                                                                                            
gCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.c..c                                                                                            <  2:293605/101‑1 (MQ=255)
  tCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.c..ctc                                                                                          <  1:1398804/101‑3 (MQ=255)
  tCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.c..ctc                                                                                          >  2:6920115/1‑99 (MQ=255)
    aaaCGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.c..ctcgg                                                                                        >  2:8964683/1‑97 (MQ=255)
       cgcggcgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.c..ctcggctt                                                                                     <  2:1129019/101‑8 (MQ=255)
        gcggcgcggTGGCGATGGAGGGCACGAAGGACAGCCTTTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.c..ctcggcttc                                                                                    <  2:2170039/101‑9 (MQ=255)
         cggcgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.c..ctcggcttcg                                                                                   >  1:5096239/1‑92 (MQ=255)
         cggcgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.c..ctcggcttcg                                                                                   <  1:7859828/101‑10 (MQ=255)
          ggcgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGc                                                                                    >  1:1917507/1‑101 (MQ=255)
          ggcgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGc                                                                                    <  1:2283037/101‑1 (MQ=255)
             gctgTGGCCATGGAGGACACAACGGCCAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTc                                                                                 <  2:9078654/98‑1 (MQ=255)
              cggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCa                                                                                >  2:8455570/1‑101 (MQ=255)
               ggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTGCCCCC.C..CGGCTTCGCCTccc                                                                               >  1:2059926/1‑99 (MQ=255)
               ggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCAc                                                                               >  2:8088535/1‑101 (MQ=255)
               ggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCAc                                                                               <  2:185527/101‑1 (MQ=255)
                gTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTGCCCCC.C..CGGCTTCGCCTCAcc                                                                              >  2:2662511/1‑101 (MQ=255)
                gTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCAcc                                                                              <  1:6027879/101‑1 (MQ=255)
                gTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCAcc                                                                              <  1:587766/101‑1 (MQ=255)
                 tGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCAccc                                                                             >  1:3817889/1‑101 (MQ=255)
                  ggCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCAcccc                                                                            >  2:5521911/1‑101 (MQ=255)
                   gCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCAccccc                                                                           <  1:2491515/101‑1 (MQ=39)
                   gCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCAccccc                                                                           <  1:1297779/101‑1 (MQ=39)
                      aTGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCggg                                                                        <  1:100004/101‑1 (MQ=39)
                       tGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGa                                                                       <  2:2463531/101‑1 (MQ=39)
                        ggAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGat                                                                      >  1:4815879/1‑101 (MQ=38)
                        ggAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGat                                                                      <  2:202730/101‑1 (MQ=38)
                            ggCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATAttt                                                                  <  1:3319371/101‑1 (MQ=38)
                            ggCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATAttt                                                                  <  1:7019745/101‑1 (MQ=38)
                            ggCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCCCCCCCCCCCCGGGGTAttt                                                                  >  1:3446084/1‑101 (MQ=255)
                               aCGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTggg                                                               <  1:7450674/101‑1 (MQ=38)
                               aCGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTggg                                                               >  2:3276948/1‑101 (MQ=38)
                                 gAAGGACAGCCTGTCCCGCGCAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGCGGc                                                             <  1:8822476/101‑1 (MQ=37)
                                 gAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGc                                                             >  1:7707292/1‑101 (MQ=38)
                                 gAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGc                                                             >  1:6608796/1‑101 (MQ=38)
                                 gAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGc                                                             <  1:8716824/101‑1 (MQ=38)
                                   aGGACAGCCTGTCCCGCGAAACCCTGCGCT.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCaa                                                           >  1:6832282/1‑101 (MQ=37)
                                     gACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGa                                                         <  2:388684/101‑1 (MQ=37)
                                     gACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGa                                                         <  2:2785319/101‑1 (MQ=37)
                                       cAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.a                                                      <  1:8595205/101‑1 (MQ=37)
                                       cAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.a                                                      <  2:1258405/101‑1 (MQ=37)
                                       cAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGAGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCTTCACCCCCGGGATATCTGGGGCAAGAT.a                                                      <  1:8078213/101‑1 (MQ=25)
                                         gCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.aaa                                                    <  2:5174144/101‑1 (MQ=37)
                                         gCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.aaa                                                    <  2:4341223/101‑1 (MQ=37)
                                          ccTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAc                                                   <  1:1731315/101‑1 (MQ=37)
                                           cTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAcc                                                  <  1:2565083/101‑1 (MQ=37)
                                           cTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAcc                                                  >  1:4279522/1‑101 (MQ=37)
                                             gTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAcccc                                                <  1:3947258/101‑1 (MQ=37)
                                             gTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAcccc                                                >  1:8367745/1‑101 (MQ=37)
                                             gTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAcccc                                                >  1:6773749/1‑101 (MQ=37)
                                             gTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAcccc                                                >  2:4594902/1‑101 (MQ=37)
                                              tCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCa                                               <  2:2287436/101‑1 (MQ=37)
                                               cccGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACTCCAt                                              >  2:5822155/1‑101 (MQ=25)
                                               cccGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCAt                                              <  1:4488592/101‑1 (MQ=37)
                                                 cgcgAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATcc                                            <  2:1520601/101‑1 (MQ=37)
                                                   cgAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCtt                                          <  2:5942105/101‑1 (MQ=37)
                                                   ccgaaCCCCGCGCCCCCGTGGTCACCTTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCt                                           <  1:8947203/98‑1 (MQ=18)
                                                    gAAACCCTGCGCG.GCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCttt                                         <  2:5636238/101‑1 (MQ=25)
                                                    gAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCttt                                         >  2:2540410/1‑101 (MQ=37)
                                                    gAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCttt                                         <  1:5380217/101‑1 (MQ=37)
                                                    gAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACACCCGGGATATTTGGGGCAAGAT.AAACCCCATCCttt                                         >  1:8230329/1‑101 (MQ=37)
                                                     aaaCCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTc                                        >  1:3199272/1‑101 (MQ=37)
                                                      aaCCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCa                                       <  2:8354087/101‑1 (MQ=37)
                                                       aCCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCAc                                      <  1:3612252/101‑1 (MQ=37)
                                                          cTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCtt                                   >  1:6197379/1‑101 (MQ=37)
                                                               cg.cCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGc                              <  2:7706966/101‑1 (MQ=37)
                                                                   cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCaaa                           >  2:4087527/1‑101 (MQ=37)
                                                                   cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCaaa                           >  2:3090351/1‑101 (MQ=37)
                                                                    gTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAat                          <  2:8691597/101‑1 (MQ=37)
                                                                     tGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCACGAT.AAACCCCATCCTTTCACCTTGCCGCAAata                         <  1:6446695/101‑1 (MQ=37)
                                                                      ggTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAatat                        >  2:6319884/1‑101 (MQ=25)
                                                                       gTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATc                       >  2:6109875/1‑101 (MQ=25)
                                                                       gTCAGCGTTGAGGCAAGGGGGCGCGG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATc                       <  2:9147005/101‑1 (MQ=25)
                                                                        tCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATcc                      <  2:3184126/101‑1 (MQ=37)
                                                                        tCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATcc                      <  2:1707809/101‑1 (MQ=37)
                                                                         cAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATccc                     <  1:5667633/101‑1 (MQ=37)
                                                                         cAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAAGATccc                     >  1:2871257/1‑101 (MQ=25)
                                                                                tAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTTGGCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGTCGCAAATATCCCggggggg              >  2:7939470/1‑100 (MQ=25)
                                                                                  ggCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGa            <  2:1438737/101‑1 (MQ=37)
                                                                                  ggCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGa            <  2:6429722/101‑1 (MQ=37)
                                                                                  ggCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGa            <  1:1178906/101‑1 (MQ=37)
                                                                                   gCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCTCCTTGCCGCAAATATCCCGGGGGGTGaa           >  2:7228490/1‑101 (MQ=25)
                                                                                   gCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa           <  2:8247817/101‑1 (MQ=37)
                                                                                   gCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGACGCAAATATCCCGGGGGGTGaa           <  1:5522118/101‑1 (MQ=25)
                                                                                    cAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCCCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt          <  1:6320124/101‑1 (MQ=25)
                                                                                    cAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt          <  1:4053051/101‑1 (MQ=37)
                                                                                    cAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt          <  1:977965/101‑1 (MQ=37)
                                                                                    cAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt          <  1:2649153/101‑1 (MQ=37)
                                                                                    cAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt          <  2:4961040/101‑1 (MQ=37)
                                                                                    cAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt          >  2:4819297/1‑101 (MQ=25)
                                                                                     caGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt         <  2:671720/100‑1 (MQ=37)
                                                                                     aaGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt         <  2:7100228/101‑1 (MQ=37)
                                                                                     aaGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt         >  2:5416312/1‑101 (MQ=25)
                                                                                     aaGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTGCACCTTGCCGCAAATATCCCGGGGGGTGAAtt         >  1:4675434/1‑101 (MQ=25)
                                                                                      ggggggCTCTGC.CCC.C..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGG.GAATTGgcc     >  1:1522199/2‑101 (MQ=2)
                                                                                      aGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AGACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg        <  1:9084512/101‑1 (MQ=37)
                                                                                      aGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg        <  2:7861707/101‑1 (MQ=37)
                                                                                      aGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg        <  1:8455934/101‑1 (MQ=37)
                                                                                       gggggCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg       >  2:859453/1‑101 (MQ=37)
                                                                                       gggggCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg       <  2:3375960/101‑1 (MQ=37)
                                                                                         gggCTCTG..CCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGgccaaa  >  2:3568527/1‑98 (MQ=2)
                                                                                                        |                                                                                            
GCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCG.CCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCT.C..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGCC  >  NC_014034/1303732‑1303921

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG