Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,320,651 +G 99.0% intergenic (+109/+76) potI1 → / ← RCAP_rcc01247 polyamine ABC transporter permease PotI/membrane transport family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,320,6511.→G99.0% 3.1 0.0 ‑0.9404intergenic (+109/+76)potI1/RCAP_rcc01247polyamine ABC transporter permease PotI/membrane transport family protein
Reads supporting (aligned to +/- strand):  new base (228/172):  ref base (2/2):  total (230/174)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.35e-01

Only 100 of 436 total aligned reads displayed.

GATCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.A.GG..CA.GG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGCAGCGCCCCCTTGCTTACGGCAGGATC  >  NC_014034/1320555‑1320739
                                                                                                     |                                                                                          
gATCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAGGGTGAAACTAAA.AGGG..CA.gg.g                                                                                        >  2:4105334/1‑101 (MQ=37)
gATCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CA.gg.g                                                                                        <  2:7304226/101‑1 (MQ=255)
gATCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CA.gg.g                                                                                        <  2:205543/101‑1 (MQ=255)
  tCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CA.gg.ggc                                                                                      >  1:5689471/1‑99 (MQ=255)
   ccGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CA.gg.ggct                                                                                     <  2:5871417/101‑4 (MQ=255)
         cGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.gctgcgctg                                                                                >  1:767139/1‑101 (MQ=25)
           cAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.gctgcgctgac                                                                              <  1:5475027/101‑3 (MQ=25)
           cAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGcc                                                                              <  2:8525807/101‑1 (MQ=37)
           cAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGcc                                                                              <  1:7811062/101‑1 (MQ=37)
              aaaGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCtt                                                                           >  2:4148252/1‑101 (MQ=37)
               aaGGGTGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTc                                                                          <  2:915193/101‑1 (MQ=37)
               aaGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTc                                                                          <  1:8739384/101‑1 (MQ=37)
               aaGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTc                                                                          >  1:8528073/1‑101 (MQ=37)
                aGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCa                                                                         <  1:9008163/101‑1 (MQ=37)
                 ggggggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCAt                                                                        <  1:1604426/101‑1 (MQ=37)
                   ggggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCt                                                                      >  2:5578274/1‑101 (MQ=38)
                    gggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCtt                                                                     >  1:9198011/1‑101 (MQ=38)
                    gggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCtt                                                                     >  1:8096673/1‑101 (MQ=38)
                      gCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGc                                                                   >  1:7370852/1‑101 (MQ=38)
                      gCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGc                                                                   >  2:7342496/1‑101 (MQ=38)
                        tGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGAAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTTCca                                                                 >  1:2216605/1‑101 (MQ=37)
                         gTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCcac                                                                >  2:1011886/1‑101 (MQ=38)
                         gTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCcac                                                                <  2:2404700/101‑1 (MQ=38)
                         gTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCcac                                                                <  1:4458983/101‑1 (MQ=38)
                         gTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCcac                                                                <  2:4551272/101‑1 (MQ=38)
                           cTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACaa                                                              >  2:4351409/1‑101 (MQ=38)
                           cTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACaa                                                              >  2:6901344/1‑101 (MQ=38)
                            tGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCCCaaa                                                             >  2:2103801/1‑101 (MQ=37)
                            tGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CA.GGTGCTGCGCTGCCCTTCATCTTGCCACaaa                                                             >  1:6391525/1‑101 (MQ=38)
                             gCCCCCCTCTTTTGCTGCTCAAATTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAat                                                            <  2:3497572/101‑1 (MQ=38)
                             gCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAat                                                            >  1:8326222/1‑101 (MQ=38)
                             gCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCAGCTTGCCACAAat                                                            <  1:6534311/101‑1 (MQ=37)
                               cccccTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAatat                                                          >  2:2989108/1‑101 (MQ=38)
                                  ccTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATccc                                                       <  1:1814491/101‑1 (MQ=38)
                                  ccTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCAGCGCTGCCCTTCATCTTGCCACAAATATccc                                                       <  1:8189706/101‑1 (MQ=38)
                                   ctctTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCg                                                      <  1:4188465/101‑1 (MQ=38)
                                      ttttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCgggg                                                   >  1:4925912/1‑101 (MQ=39)
                                       tttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCggggg                                                  >  2:2263648/1‑101 (MQ=39)
                                       tttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCACTTCATCTTGCCACAAatatagatcgga                                                  >  2:3675621/1‑93 (MQ=37)
                                        ttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCgggggg                                                 <  1:5421588/101‑1 (MQ=38)
                                        ttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCgggggg                                                 >  2:2009478/1‑101 (MQ=38)
                                          gctgcGCAAAATTCCCCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAg                                               <  1:3433821/101‑1 (MQ=38)
                                          gctgcGCAAAATTCACCCCCCGAGGATATTTGAAGGAAGGTGAAAATGAA.AGGG..TAGGG.GATGCGCTGGCGTTCATGTTGCCACAAAAATACAGGGGGGAg                                               >  1:4297595/1‑101 (MQ=19)
                                          gctgcGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAg                                               >  1:7295054/1‑101 (MQ=38)
                                            tgcGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTc                                             <  2:6747311/101‑1 (MQ=38)
                                            tgcGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTc                                             >  2:8677392/1‑101 (MQ=38)
                                             gcgcAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAGG.GG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTcc                                            <  2:6002530/101‑1 (MQ=37)
                                                cAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGGCCCGc                                         >  1:4246155/1‑101 (MQ=37)
                                                  aaaTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAg                                       >  1:7083526/1‑101 (MQ=37)
                                                  aaaTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAg                                       <  2:5689264/101‑1 (MQ=37)
                                                  aaaTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAg                                       >  2:1233703/1‑101 (MQ=37)
                                                   aaTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAgg                                      >  2:9074895/1‑101 (MQ=37)
                                                   aaTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAgg                                      >  2:1464765/1‑101 (MQ=37)
                                                    aTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAggg                                     >  2:7706967/1‑101 (MQ=37)
                                                    aTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAggg                                     >  2:1777556/1‑101 (MQ=37)
                                                     ttCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGa                                    >  1:4037668/1‑101 (MQ=37)
                                                      tCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAc                                   <  2:1894801/101‑1 (MQ=37)
                                                      tCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAc                                   <  1:3952688/101‑1 (MQ=37)
                                                      tCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAc                                   <  1:6759693/101‑1 (MQ=37)
                                                       cACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g                                 <  1:1035771/101‑1 (MQ=25)
                                                        aCCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACCAATATCCCGGGGGGAGTCCCGCAGGGAC.gg                                >  1:3725289/1‑101 (MQ=25)
                                                        aCCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gg                                <  2:5036051/101‑1 (MQ=25)
                                                            cccGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gggggg                            <  1:8860412/101‑2 (MQ=25)
                                                             gatctgATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggc                           <  2:8913073/96‑3 (MQ=21)
                                                             ccGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggc                           >  2:7954155/1‑99 (MQ=21)
                                                             ccGAGGATATTTGAAGCAAGGTGAAACTGAA.A.GGGCCG.GG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggc                           <  2:7011919/101‑3 (MQ=21)
                                                              cGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggca                          >  2:8119353/1‑98 (MQ=21)
                                                              cGAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAACATCCCGGGGGGAGTCCCGCAGGGAC.ggggggca                          <  1:4768620/101‑4 (MQ=21)
                                                               gAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcag                         <  1:1896486/101‑5 (MQ=21)
                                                               gAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcag                         <  1:315156/101‑5 (MQ=21)
                                                               gAGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcag                         >  2:389381/1‑97 (MQ=21)
                                                                aGGATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcagc                        >  2:2727147/1‑96 (MQ=21)
                                                                 ggATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAgcg                        >  2:5239018/1‑101 (MQ=18)
                                                                 ggATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAgcg                        <  2:1383982/101‑1 (MQ=18)
                                                                 ggATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAgcg                        >  1:8013183/1‑101 (MQ=18)
                                                                 ggATATTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAgcg                        <  1:2752331/101‑1 (MQ=18)
                                                                   atatTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGcc                      >  2:3386623/1‑101 (MQ=18)
                                                                   atatTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGcc                      >  1:7578527/1‑101 (MQ=18)
                                                                   atatGTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGcc                      <  1:2980618/101‑1 (MQ=18)
                                                                    tatTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGccc                     >  2:7778095/1‑101 (MQ=18)
                                                                     atTTGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGcccc                    >  1:5922038/1‑101 (MQ=18)
                                                                      tttGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGccccc                   <  2:252367/101‑1 (MQ=18)
                                                                       ttGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCt                  >  1:2067383/1‑101 (MQ=18)
                                                                       ttGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCt                  <  2:6474365/101‑1 (MQ=18)
                                                                        tGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCtt                 <  1:3617926/101‑1 (MQ=18)
                                                                        tGAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCtt                 >  1:6157049/1‑101 (MQ=18)
                                                                         gAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTg                <  2:7622179/101‑1 (MQ=18)
                                                                         gAAGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTg                <  2:4005686/101‑1 (MQ=18)
                                                                           aGCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCt              >  1:1328299/1‑101 (MQ=18)
                                                                            gCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCtt             <  1:2616802/101‑1 (MQ=18)
                                                                            gCAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCtt             >  2:4730807/1‑101 (MQ=18)
                                                                             cAAGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTa            >  1:1485895/1‑101 (MQ=18)
                                                                              aaGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTAc           >  2:8344539/1‑101 (MQ=18)
                                                                              aaGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTAc           >  1:8875180/1‑101 (MQ=18)
                                                                               aGGTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACg          >  1:5572525/1‑101 (MQ=18)
                                                                                ggTGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACgg         <  1:6901629/101‑1 (MQ=18)
                                                                                  tGAAACTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCa       >  1:1154803/1‑101 (MQ=18)
                                                                                      aCTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGa  >  2:2859576/1‑100 (MQ=18)
                                                                                       cTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATc  <  1:5164870/101‑1 (MQ=18)
                                                                                       cTGAA.AGGG..CAGGG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATc  >  1:2329095/1‑101 (MQ=18)
                                                                                                     |                                                                                          
GATCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAA.A.GG..CA.GG.GCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGCAGCGCCCCCTTGCTTACGGCAGGATC  >  NC_014034/1320555‑1320739

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG