Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,320,707 +G 100% intergenic (+165/+20) potI1 → / ← RCAP_rcc01247 polyamine ABC transporter permease PotI/membrane transport family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,320,7071.→G100.0% 1215.0 393intergenic (+165/+20)potI1/RCAP_rcc01247polyamine ABC transporter permease PotI/membrane transport family protein
Reads supporting (aligned to +/- strand):  new base (219/173):  ref base (0/1):  total (219/174)

Only 100 of 453 total aligned reads displayed.

TCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAA.GGCA.GGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTCGACGCCGCC  >  NC_014034/1320609‑1320802
                                                                                                      |                                                                                               
 cACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g                                                                                                <  1:1035771/101‑1 (MQ=25)
     atctgAGGATATTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggg                                                                                            <  2:396692/97‑1 (MQ=21)
      cccGAGGATATTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gggggg                                                                                           <  1:8860412/101‑2 (MQ=25)
       gatctgATATTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggc                                                                                          <  2:8913073/96‑3 (MQ=21)
       ccGAGGATATTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggc                                                                                          >  2:771907/1‑99 (MQ=21)
         gAGGATATTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcag                                                                                        >  2:1800405/1‑97 (MQ=21)
         gAGGATATTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcag                                                                                        <  1:1893477/101‑5 (MQ=21)
         gAGGATATTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gggggagat                                                                                        >  1:396700/1‑97 (MQ=21)
          aGGATATTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcagc                                                                                       >  2:2727147/1‑96 (MQ=21)
          aGGATATTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTACCGCAGGGAC.ggggggcagc                                                                                       >  1:7251264/1‑96 (MQ=21)
           ggATATTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAgcg                                                                                       <  1:4805436/101‑1 (MQ=18)
            gATATTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAgcgc                                                                                      >  2:4470823/1‑101 (MQ=18)
            gATATTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAgcgc                                                                                      >  1:3695462/1‑101 (MQ=18)
             atatTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCGACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGcc                                                                                     <  1:5004107/101‑1 (MQ=18)
             atatTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGcc                                                                                     <  2:7302666/101‑1 (MQ=18)
             atatTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGcc                                                                                     <  1:1548529/101‑1 (MQ=18)
              tatTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGccc                                                                                    >  2:9176685/1‑101 (MQ=18)
              tatTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGccc                                                                                    >  1:8168903/1‑101 (MQ=18)
              tatTTGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGccc                                                                                    >  2:7778095/1‑101 (MQ=18)
                tttGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGccccc                                                                                  >  2:6672744/1‑101 (MQ=18)
                 ttGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCt                                                                                 >  1:2067383/1‑101 (MQ=18)
                 ttGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCt                                                                                 <  2:6474365/101‑1 (MQ=18)
                 ttGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCt                                                                                 >  1:6665510/1‑101 (MQ=18)
                 ttGAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCt                                                                                 >  1:8777319/1‑101 (MQ=18)
                   gAAGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTg                                                                               >  2:5784753/1‑101 (MQ=18)
                    aaGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGc                                                                              >  2:3736075/1‑101 (MQ=18)
                     aGCAAGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCt                                                                             <  2:5620469/101‑1 (MQ=18)
                     aGCAAGGTGAAACTGAAAGGGCA.GGGCCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCt                                                                             <  2:5949687/101‑1 (MQ=18)
                        aaGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTAc                                                                          <  2:2189747/101‑1 (MQ=18)
                        aaGGTGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTAc                                                                          <  2:2043914/101‑1 (MQ=18)
                         agttGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACg                                                                         >  1:3121232/4‑101 (MQ=17)
                         aGGTGAAACTGAAAGGGCAGGGG.CGGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACg                                                                         <  2:1498118/101‑1 (MQ=17)
                            tGAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCa                                                                      >  1:5303497/1‑101 (MQ=18)
                             gAAACTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAg                                                                     >  2:3257683/1‑101 (MQ=18)
                              aaaCTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAgg                                                                    >  2:6187148/1‑101 (MQ=18)
                              aaaCTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAgg                                                                    >  2:3391731/1‑101 (MQ=18)
                               aaCTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAggg                                                                   >  2:4108424/1‑100 (MQ=18)
                                aCTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGac                                                                  >  2:2859576/1‑100 (MQ=18)
                                 cTGAAAGGGCAGGGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATc                                                                 >  1:2329095/1‑101 (MQ=18)
                                   gaaagggcagggg.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGGTcgc                                                               >  1:2988422/11‑101 (MQ=18)
                                   gaaagggcagggg.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGGTcgc                                                               >  1:7571006/11‑101 (MQ=18)
                                   gaaagggcagggg.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATcgc                                                               >  2:4711214/11‑101 (MQ=18)
                                    aaagggcagggg.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATcgcg                                                              >  2:5016893/10‑101 (MQ=18)
                                      agggcagggg.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGGTcgcggg                                                            >  1:5092797/8‑99 (MQ=18)
                                      agggcagggg.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAg                                                            >  1:3524117/8‑101 (MQ=18)
                                        ggcagggg.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGGGcc                                                          >  2:7045794/6‑101 (MQ=18)
                                        ggcagggg.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGcc                                                          <  1:678686/96‑1 (MQ=255)
                                        ggcagcgg.CTGCGCTGCCCTTCATCTTGCCACAAAGATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGcc                                                          <  1:761645/95‑1 (MQ=18)
                                          cagggg.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAg                                                        <  1:2250284/98‑1 (MQ=255)
                                           agggg.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAgc                                                       >  2:8696743/3‑100 (MQ=255)
                                            gggg.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGGTTGCGAGCCAGAc                                                      >  2:1314449/2‑101 (MQ=18)
                                            gggg.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGAc                                                      <  2:1743929/100‑1 (MQ=255)
                                             ggg.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGAcc                                                     >  1:7385516/1‑101 (MQ=255)
                                              gg.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGAccc                                                    <  2:538418/101‑1 (MQ=255)
                                               g.ctgcgctgcCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCa                                                   <  2:3490793/101‑1 (MQ=255)
                                                  tgcgctgcCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGa                                                 <  1:2773139/101‑1 (MQ=255)
                                                    cgctgcCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATc                                               <  1:3842059/101‑1 (MQ=255)
                                                        gcCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCCGACCCAGATCGAcc                                           >  1:1277381/1‑100 (MQ=255)
                                                          ccTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGGGCGCGAGGCGGACCCAGATCGACAgg                                         >  1:8497162/1‑101 (MQ=18)
                                                           cTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGa                                        >  1:4128832/1‑101 (MQ=255)
                                                            ttCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGGTCGCGAGCCAGACCCAGATCGACAGGAt                                       >  1:323255/1‑101 (MQ=255)
                                                              cATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGGTCGCGAGCCAGACCCAGATCGACAGGATCg                                     >  1:5676288/1‑101 (MQ=255)
                                                              cATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCg                                     <  1:4351533/101‑1 (MQ=255)
                                                              cATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCg                                     <  1:1777609/101‑1 (MQ=255)
                                                              cATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCg                                     >  1:3459094/1‑101 (MQ=255)
                                                               aTCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGGTCga                                    >  2:6572881/1‑101 (MQ=255)
                                                                tCTTGCCACAAATGTCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCGCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCgag                                   <  1:5567588/101‑1 (MQ=25)
                                                                tCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCgag                                   <  2:8012843/101‑1 (MQ=255)
                                                                tCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCgag                                   <  1:9131720/101‑1 (MQ=255)
                                                                 cTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGCCCCAGATCGACCGGAACgaga                                  >  1:1423795/1‑101 (MQ=21)
                                                                   tGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATTGCGAGCCAGACCCAGATCGACAGGATCGagagg                                >  2:677940/1‑100 (MQ=255)
                                                                   tGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCCGACCCAGATCGACAGGATCCAGAgc                                >  2:2585906/1‑101 (MQ=25)
                                                                   tGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAgc                                >  1:4192616/1‑101 (MQ=255)
                                                                   tGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAgc                                <  2:6974978/101‑1 (MQ=255)
                                                                      cacaAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGcgcg                             <  1:7749941/101‑1 (MQ=255)
                                                                       acaAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCgg                            >  1:1727629/1‑101 (MQ=255)
                                                                          aaTATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGGgg                         >  2:76574/1‑101 (MQ=255)
                                                                          aaTATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCCAGAgcgccggtg                         >  2:1150047/1‑96 (MQ=25)
                                                                            tatCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCa                       <  1:5177452/101‑1 (MQ=255)
                                                                            tatCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCa                       <  1:6301089/101‑1 (MQ=255)
                                                                              tCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAAt                     <  2:8552835/101‑1 (MQ=255)
                                                                              tCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAAt                     <  2:8137734/101‑1 (MQ=255)
                                                                               gcgggggtgAGTCCGGCAGGGACGGGGGGCTGCGCCCCCTTGCTTACGGCAGTATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATc                    <  1:8957522/98‑1 (MQ=255)
                                                                               cccGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATc                    <  1:1119554/101‑1 (MQ=255)
                                                                                 cGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAg                  <  1:5879893/101‑1 (MQ=255)
                                                                                 cGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGGGGCAATCAg                  >  1:6212040/1‑101 (MQ=255)
                                                                                  ggggggAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGc                 >  2:3048536/1‑101 (MQ=255)
                                                                                   gggggAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGGGGCAATCAGcc                >  2:7464602/1‑100 (MQ=255)
                                                                                    ggggAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCAc               <  2:488465/101‑1 (MQ=255)
                                                                                      ggAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGc             >  1:8347424/1‑101 (MQ=255)
                                                                                       gAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCt            <  1:7954495/101‑1 (MQ=255)
                                                                                        aGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTc           <  2:4260194/101‑1 (MQ=255)
                                                                                         gTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTCg          >  1:7010380/1‑101 (MQ=255)
                                                                                          tCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTCGa         <  2:8305746/101‑1 (MQ=255)
                                                                                            ccGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTCGAcg       <  1:4471319/101‑1 (MQ=255)
                                                                                             cGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTCGAcgc      <  2:5237807/101‑1 (MQ=255)
                                                                                              gCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTCGAcgcc     >  1:2581610/1‑101 (MQ=255)
                                                                                                aGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGGGGCAATCAGCACGCTCGAcgccgc   >  1:4673078/1‑101 (MQ=255)
                                                                                                aGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGGGGCAATCAGCACGCCCGAcgccgc   >  2:8820698/1‑101 (MQ=255)
                                                                                                 gggACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTCGACgccgcc  >  2:8454096/1‑101 (MQ=255)
                                                                                                      |                                                                                               
TCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAA.GGCA.GGG.CTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGCGAGCCAGACCCAGATCGACAGGATCGAGAGCGCGGTGGCAATCAGCACGCTCGACGCCGCC  >  NC_014034/1320609‑1320802

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG