Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,867 +T 1.7% intergenic (+679/‑252) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8671.→T 1.7% 28.3 ‑2.2 ‑0.6572intergenic (+679/‑252)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/8):  ref base (266/296):  total (268/304)
Fisher's exact test strand distribution p-value = 1.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.25e-01

Only 100 of 616 total aligned reads displayed.

GCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTC  >  NC_014034/1395767‑1395967
                                                                                                     |                                                                                                            
  tCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcg.cc                                                                                                            >  1:5502444/1‑101 (MQ=255)
   ccccccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcg.ccg                                                                                                           >  2:7172555/1‑101 (MQ=255)
     ccccGCGGGGGCGTGGATCGAAACCTGCGGCGTGGGTTGCCAGTGATAGACGCCGGGGACGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACTGcg.ccgac                                                                                                         >  2:2865941/1‑99 (MQ=255)
     ccccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCg.ccgc.c                                                                                                        >  2:761426/1‑101 (MQ=255)
       ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCT.CCGC.CTa                                                                                                      >  2:2100441/1‑101 (MQ=255)
        cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTAt                                                                                                     <  2:3775159/101‑1 (MQ=255)
        cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTAt                                                                                                     <  1:6874812/101‑1 (MQ=255)
        cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTAt                                                                                                     <  2:3326531/101‑1 (MQ=255)
        cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTAt                                                                                                     <  1:3567342/101‑1 (MQ=255)
         gcgGGGGGGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATc                                                                                                    <  1:7418110/101‑1 (MQ=255)
         gcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATc                                                                                                    <  1:6700164/101‑1 (MQ=255)
           gggggCGTGGATCGAAACCTGCGGCGTGATTTGCCAGTAATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCg.ccgc.ccgcctt                                                                                                  <  2:5693325/101‑7 (MQ=37)
           gggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGc                                                                                                  >  2:6218048/1‑101 (MQ=255)
           gggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGc                                                                                                  >  2:5623351/1‑101 (MQ=255)
             gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                                >  2:6641853/1‑101 (MQ=255)
             gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                                <  1:6071068/101‑1 (MQ=255)
              ggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATc                                                                                               <  1:8550700/101‑1 (MQ=255)
               gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATc.c                                                                                             <  2:3149056/101‑1 (MQ=255)
                 gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.Cgg                                                                                           >  1:3762810/1‑101 (MQ=255)
                 gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.Cgg                                                                                           <  1:5355878/101‑1 (MQ=255)
                      tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAg                                                                                   >  1:1279011/1‑101 (MQ=255)
                       cGAACCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAgc                                                                                  <  1:4543372/101‑1 (MQ=255)
                       cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAgc                                                                                  >  2:8139903/1‑101 (MQ=255)
                       cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAgc                                                                                  <  1:971677/101‑1 (MQ=255)
                        gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAgcg                                                                                 >  2:4696630/1‑101 (MQ=255)
                         aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAgcgc                                                                                <  2:1947908/101‑1 (MQ=255)
                                   cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCg                                                                   <  2:5233996/101‑1 (MQ=255)
                                   cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCg                                                                   >  1:4937915/1‑101 (MQ=255)
                                   cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCg                                                                   >  2:7963118/1‑101 (MQ=255)
                                   cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCg                                                                   <  1:3721431/101‑1 (MQ=255)
                                    gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGc                                                                  <  2:486955/101‑1 (MQ=255)
                                     tGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCt                                                                 >  2:6776599/1‑101 (MQ=255)
                                         ttGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTcccc                                                             <  1:4196794/101‑1 (MQ=255)
                                           gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTcccccc                                                           >  1:6588591/1‑101 (MQ=255)
                                             cAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCcgc                                                         >  2:1757179/1‑101 (MQ=255)
                                              aGTGATAGACGCCGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCcgcg                                                        <  2:2560174/101‑1 (MQ=255)
                                                 gATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCgggg                                                     >  1:5235241/1‑101 (MQ=255)
                                                  aTAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCggggg                                                    >  1:7884566/1‑101 (MQ=255)
                                                    aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                  <  1:1186412/101‑1 (MQ=255)
                                                    aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GGCGCTCCCCCCGCGGGGGCg                                                  >  2:8822694/1‑101 (MQ=255)
                                                     gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGt                                                 <  1:6737475/101‑1 (MQ=255)
                                                      aCGCCGTGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTg                                                >  2:8283198/1‑101 (MQ=255)
                                                       gctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACA.CCGG.GGCTCTGATCACGG...T.TTGGGAC.G.G..A.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  1:224643/91‑1 (MQ=2)
                                                       gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTgg                                               <  1:8998663/91‑1 (MQ=9)
                                                       gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTgg                                               >  2:1444781/11‑101 (MQ=9)
                                                       ccggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCg                                                  <  1:5122654/92‑1 (MQ=2)
                                                        cggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGt                                                 <  2:160312/93‑1 (MQ=2)
                                                         caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                             >  1:1690911/9‑101 (MQ=9)
                                                          aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:4827817/94‑1 (MQ=9)
                                                           gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  1:2075981/101‑1 (MQ=255)
                                                           acctatgTCGCTCGCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  1:6967103/95‑1 (MQ=9)
                                                             ggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  2:450020/101‑1 (MQ=255)
                                                             ggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  2:3037912/101‑1 (MQ=255)
                                                             gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATT.C.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  2:7023721/98‑1 (MQ=2)
                                                             gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATT.C.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  1:1003239/98‑1 (MQ=2)
                                                             cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:3579659/98‑1 (MQ=2)
                                                             cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:3355431/98‑1 (MQ=2)
                                                              ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  1:3102999/99‑1 (MQ=2)
                                                              gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        <  1:6224343/101‑1 (MQ=255)
                                                              cccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATT.C.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          >  1:2657028/3‑101 (MQ=2)
                                                               ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATT.C.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  2:6129773/100‑1 (MQ=2)
                                                               atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       <  1:8674191/99‑1 (MQ=9)
                                                               aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       >  2:4307400/1‑101 (MQ=255)
                                                               aCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       <  2:2230836/101‑1 (MQ=255)
                                                                tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                      <  1:3919646/100‑2 (MQ=9)
                                                                cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                      <  1:8265134/101‑1 (MQ=255)
                                                                cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                      <  2:6404797/101‑1 (MQ=255)
                                                                  tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                    <  1:8385311/101‑1 (MQ=9)
                                                                  tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                    <  1:5887491/101‑1 (MQ=9)
                                                                  gcGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                    <  2:591697/100‑1 (MQ=255)
                                                                    gCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc                                  >  1:2490079/1‑101 (MQ=9)
                                                                    gCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc                                  <  2:8997320/101‑1 (MQ=9)
                                                                    gCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc                                  <  2:1290315/101‑1 (MQ=9)
                                                                     ccccccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                 <  1:8063356/99‑1 (MQ=255)
                                                                     cTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACAATGCg                                 >  2:4035197/1‑101 (MQ=9)
                                                                      tCCGCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGACGTGGATCGAAACGATGCGt                                >  1:5619794/1‑101 (MQ=255)
                                                                      cccccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                                <  2:5419226/100‑1 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAACTTCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                               <  2:5102342/101‑1 (MQ=255)
                                                                        cccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                              <  1:1940832/101‑4 (MQ=9)
                                                                        accccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                              >  2:8566572/2‑98 (MQ=9)
                                                                           ccGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                              <  1:1465877/101‑10 (MQ=2)
                                                                            cgcgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                          <  1:7560309/101‑1 (MQ=255)
                                                                            cgcgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                          <  2:6585766/101‑1 (MQ=255)
                                                                               gggggCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcggtcct                       <  1:7210968/101‑11 (MQ=2)
                                                                               gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                       <  2:7241347/101‑1 (MQ=255)
                                                                               gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                       <  1:2833501/101‑1 (MQ=255)
                                                                                 gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                     <  1:4372644/101‑1 (MQ=255)
                                                                                 gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                     <  1:7676890/101‑1 (MQ=255)
                                                                                 gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                     >  1:8806101/1‑101 (MQ=255)
                                                                                   gCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                   <  2:2368536/101‑1 (MQ=255)
                                                                                    cGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                  <  2:2724425/101‑1 (MQ=255)
                                                                                    cGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                  >  1:4640782/1‑101 (MQ=255)
                                                                                     gTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                 >  2:6313615/1‑101 (MQ=255)
                                                                                      tGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                >  2:6473552/1‑101 (MQ=255)
                                                                                           cGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt           >  1:2978713/1‑101 (MQ=255)
                                                                                           cGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt           >  2:77103/1‑101 (MQ=255)
                                                                                           cGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt           >  2:8506744/1‑101 (MQ=255)
                                                                                            gAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg          >  2:5500932/1‑101 (MQ=255)
                                                                                            gAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg          >  2:121219/1‑101 (MQ=255)
                                                                                               aCTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAATCGATGCGTTCATGGCCTACGTCGGGCAGTGGAc       <  1:6442970/101‑1 (MQ=255)
                                                                                                     |                                                                                                            
GCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTC  >  NC_014034/1395767‑1395967

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG