Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,031 +G 16.3% intergenic (+843/‑88) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0312.→G16.3% 261.0 ‑0.6 ‑0.1484intergenic (+843/‑88)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (35/44):  ref base (195/210):  total (230/254)
Fisher's exact test strand distribution p-value = 5.41e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.32e-01

Only 100 of 524 total aligned reads displayed.

GATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT  >  NC_014034/1395931‑1396131
                                                                                                         |                                                                                                     
 aTGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCTCGGGCGG.GTGGATCGAAACTCGCCTTCGATCCGGGCCGGCCTC.CCGCCT.GC.cgc..g                                                                                                     >  1:9086805/1‑101 (MQ=37)
 aTGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.cgc..g                                                                                                     >  1:6734391/1‑101 (MQ=255)
   gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTc                                                                                                   >  1:1434443/1‑101 (MQ=255)
   gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTc                                                                                                   >  2:3292516/1‑101 (MQ=255)
   gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTc                                                                                                   <  2:1364916/101‑1 (MQ=255)
    cGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCg                                                                                                  <  2:3184304/101‑1 (MQ=255)
     gTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGc                                                                                                 >  1:8738586/1‑101 (MQ=255)
       tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTc                                                                                               >  2:4061335/1‑101 (MQ=255)
        cATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTcc                                                                                              <  2:4101956/101‑1 (MQ=255)
           ggCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTccccc                                                                                           <  1:8355748/101‑1 (MQ=255)
            gCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTcccccc                                                                                          <  1:8140252/101‑1 (MQ=255)
            gCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTcccccc                                                                                          <  2:4703270/101‑1 (MQ=255)
              cTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCcgc                                                                                        >  1:7800799/1‑101 (MQ=255)
                 cGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTGCCCCcgcgcg                                                                                     >  2:3413008/1‑99 (MQ=255)
                    cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGc                                                                                  <  1:4818303/101‑1 (MQ=255)
                      ggCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGt                                                                                >  1:7466198/1‑101 (MQ=255)
                      ggCAGTGGACTGGTCGCTCCCCCCCCGGGGGCGGGGGACCGAAACCCCCCCTCG.TCCGCGGCTTCCTC.ACCCCTGGC.CCC..GCCCCCCCCCCCCCggggcgg                                                                                 >  1:3863660/1‑98 (MQ=255)
                         aGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                             <  1:4919727/101‑1 (MQ=255)
                         aGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                             >  1:7882265/1‑101 (MQ=255)
                         aGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                             >  1:3125235/1‑101 (MQ=255)
                          tctgcgcggTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                             <  2:824873/94‑1 (MQ=2)
                           tGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                           <  2:495620/101‑1 (MQ=255)
                           tGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTGGCCGC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                           <  1:5423499/101‑1 (MQ=255)
                           gggACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                           <  1:1049657/100‑1 (MQ=255)
                           ctgcgcggTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTCGTATTGT.GCGCTG..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                            >  2:641327/7‑101 (MQ=2)
                            tgcgcggTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                           <  1:19931/96‑1 (MQ=9)
                            tgcgcggTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                           <  1:8859178/96‑1 (MQ=9)
                            tgcgcggTCCCTGCCCCCGCGGGGGC.CTGGATCCAAACTCGGCCTCGATCCGATC.AGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                           <  2:5301786/96‑1 (MQ=2)
                            ggACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                          >  2:5239120/1‑101 (MQ=255)
                            ggACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                          >  1:6520424/1‑101 (MQ=255)
                            ggACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                          >  1:7285038/1‑101 (MQ=255)
                            ggACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGCGGATCg                                                                          >  2:6188057/1‑101 (MQ=255)
                             gcgcggTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                          <  2:362679/97‑1 (MQ=9)
                              aCTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                        <  1:5682072/101‑1 (MQ=255)
                              aCTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                        <  1:8480181/101‑1 (MQ=255)
                               cTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                       >  2:4889813/1‑101 (MQ=255)
                                cggTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                       <  2:6945872/100‑1 (MQ=2)
                                cggTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                       <  2:2247879/100‑1 (MQ=2)
                                 ggTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                      <  1:3229322/101‑1 (MQ=9)
                                 ggTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                      <  2:4459135/101‑1 (MQ=2)
                                 ggTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                      >  1:5153240/1‑101 (MQ=9)
                                 ggTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                                     <  1:121219/101‑1 (MQ=255)
                                  gTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                                     >  1:978268/1‑100 (MQ=2)
                                  gTCGCGCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                                    <  2:3774205/101‑1 (MQ=255)
                                   tCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActt                                                                    <  2:1879601/101‑3 (MQ=2)
                                    cGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                                                  <  2:4607404/101‑1 (MQ=255)
                                    cGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                                                  >  2:8741892/1‑101 (MQ=255)
                                    cGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                                                  >  1:1664640/1‑101 (MQ=255)
                                      cTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgat                                                                 <  1:61432/101‑6 (MQ=2)
                                       tCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                                                >  1:4189848/1‑95 (MQ=2)
                                       tCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                                                >  2:7960895/1‑95 (MQ=2)
                                        ccccccGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                              >  1:2564854/1‑101 (MQ=255)
                                         cccccGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgac                                                              <  1:6245237/101‑9 (MQ=2)
                                           cccGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacca                                                            <  2:8894215/101‑11 (MQ=2)
                                                gggggC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                                      <  2:2043693/101‑1 (MQ=255)
                                                 ggggC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                                     <  2:3110036/101‑1 (MQ=255)
                                                  gggC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                                                    <  1:7614403/101‑1 (MQ=255)
                                                   ggC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                                   >  1:2153321/1‑101 (MQ=255)
                                                   ggC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                                   <  1:635570/101‑1 (MQ=255)
                                                    ggcgtgatccGAACCTCCCACGCGGTCCGCGCTTGGCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                                   <  2:8519548/92‑1 (MQ=25)
                                                    gC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                                                  <  2:7884233/101‑1 (MQ=255)
                                                    gC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                                                  <  2:3905608/101‑1 (MQ=255)
                                                     c.GTGGATCGAACCTCGCCCTCGATCCGCGCTTGCCTC.ACCCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGc                                                 <  1:2932466/101‑1 (MQ=255)
                                                       gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                                                >  1:5854349/1‑101 (MQ=255)
                                                         ggATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCg                                              <  2:1443563/101‑1 (MQ=255)
                                                         ggATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCg                                              >  2:7459863/1‑101 (MQ=255)
                                                            tCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTg                                           <  2:1153832/101‑1 (MQ=255)
                                                            tCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTg                                           >  2:352751/1‑101 (MQ=255)
                                                             cGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GCCCCTCCCCCCGCGGGGGCGGGGATCGAAACAGGCGCATCGGAGGTATATGCCCGGTgg                                          >  2:6129579/1‑100 (MQ=38)
                                                              gAAAATCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                                         >  2:9108756/1‑101 (MQ=255)
                                                               aaaCTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                                        >  2:5396106/1‑101 (MQ=255)
                                                               aaaCTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                                        <  2:1857188/101‑1 (MQ=255)
                                                                accTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                                       <  2:673548/99‑1 (MQ=255)
                                                                aaCTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                                       <  2:5901397/101‑1 (MQ=255)
                                                                 aCTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg                                      <  1:278130/101‑1 (MQ=255)
                                                                 aCTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg                                      <  1:4409550/101‑1 (MQ=255)
                                                                    cGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt                                   >  2:7143410/1‑101 (MQ=255)
                                                                     gCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTg                                  <  2:629831/101‑1 (MQ=255)
                                                                           gATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTc                            >  1:6937142/1‑101 (MQ=255)
                                                                            aTCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcc                           <  1:4312944/101‑1 (MQ=255)
                                                                            aTCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcc                           >  1:7096394/1‑101 (MQ=255)
                                                                            aTCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcc                           >  1:1801372/1‑101 (MQ=255)
                                                                              ccGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccc                         <  2:2978615/101‑1 (MQ=255)
                                                                                   cTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgcg                    >  2:7626832/1‑101 (MQ=255)
                                                                                     tGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCggg                  >  1:4034553/1‑101 (MQ=255)
                                                                                        cTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc               <  2:5560110/101‑1 (MQ=255)
                                                                                            aCGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTg            <  1:6659952/101‑1 (MQ=255)
                                                                                              gCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGa          <  2:6653044/101‑1 (MQ=255)
                                                                                               ccT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt         <  2:2650092/101‑1 (MQ=255)
                                                                                               ccT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt         >  1:1145830/1‑101 (MQ=255)
                                                                                                cT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATc        <  1:6024875/101‑1 (MQ=255)
                                                                                                cT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATc        >  2:1751091/1‑101 (MQ=255)
                                                                                                 t.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCg       <  2:5688967/101‑1 (MQ=255)
                                                                                                 t.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCg       <  1:1271380/101‑1 (MQ=255)
                                                                                                  cgC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCg       <  2:5125080/100‑1 (MQ=255)
                                                                                                    c.cGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     <  2:7296418/101‑1 (MQ=255)
                                                                                                      cgc..gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa    >  1:5009125/1‑101 (MQ=255)
                                                                                                      cgc..gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa    >  1:3404929/1‑101 (MQ=255)
                                                                                                        c..gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt  >  1:3606976/1‑101 (MQ=255)
                                                                                                        c..gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt  >  2:289197/1‑101 (MQ=255)
                                                                                                         |                                                                                                     
GATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT  >  NC_014034/1395931‑1396131

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG