Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,469 +A 13.9% coding (1351/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,4691.→A13.9% 293.4 ‑1.6 ‑0.4503coding (1351/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (34/36):  ref base (249/184):  total (283/220)
Fisher's exact test strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.78e-01

Only 100 of 551 total aligned reads displayed.

TCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTC  >  NC_014034/1397369‑1397569
                                                                                                     |                                                                                                       
tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCg                                                                                                          >  2:7230635/1‑101 (MQ=255)
  gCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.tc                                                                                                       >  1:1911839/1‑101 (MQ=255)
    tCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.tcat                                                                                                     <  1:3935587/101‑1 (MQ=255)
    cccccccGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.tcat                                                                                                     <  2:6854439/100‑1 (MQ=255)
        cccGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.acc                                                                                                <  1:8346796/101‑1 (MQ=255)
          cgcgGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.accac                                                                                              >  1:530797/1‑101 (MQ=255)
           gcgGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACg                                                                                             <  2:308831/101‑1 (MQ=255)
            cgGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGc                                                                                            >  1:7406600/1‑101 (MQ=255)
             gggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCa                                                                                           >  1:9181122/1‑101 (MQ=255)
             gggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCa                                                                                           >  1:959993/1‑101 (MQ=255)
             gggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCa                                                                                           >  2:8233893/1‑101 (MQ=255)
              ggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCaa                                                                                          >  1:3525689/1‑101 (MQ=255)
                ggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.cc                                                                                       <  1:7907699/101‑1 (MQ=255)
                  cGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGc                                                                                     >  1:6040003/1‑101 (MQ=255)
                   gTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGcc                                                                                    >  2:225388/1‑101 (MQ=255)
                   gTGGATCGAAACTCTTCAATCGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CTGcc                                                                                    <  1:7233508/101‑1 (MQ=255)
                    tGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGccc                                                                                   >  2:7085772/1‑101 (MQ=255)
                    tGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGccc                                                                                   >  1:4055037/1‑101 (MQ=255)
                      gATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.Tg                                                                                >  1:3438182/1‑101 (MQ=255)
                      gATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.Tg                                                                                <  1:765194/101‑1 (MQ=255)
                      gATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.Tg                                                                                >  1:3275965/1‑101 (MQ=255)
                        tCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGca                                                                              >  1:646113/1‑101 (MQ=255)
                        tCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGca                                                                              >  1:2059720/1‑101 (MQ=255)
                         cGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcac                                                                             >  1:7761620/1‑101 (MQ=255)
                          gAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcagc                                                                            >  2:930906/1‑99 (MQ=255)
                           aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcacca                                                                           <  2:132548/101‑1 (MQ=255)
                            aaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcaccac                                                                          <  1:6633158/101‑1 (MQ=255)
                            aaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcaccac                                                                          <  1:5918315/101‑1 (MQ=255)
                              ctctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCg                                                                        <  2:6390620/101‑1 (MQ=255)
                              ctctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCg                                                                        <  2:7554004/101‑1 (MQ=255)
                               tctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCgg                                                                       >  1:3780128/1‑101 (MQ=255)
                               tctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCgg                                                                       >  1:3708314/1‑101 (MQ=255)
                                ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGt                                                                      <  2:1996115/101‑1 (MQ=255)
                                ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGt                                                                      >  2:5181432/1‑101 (MQ=255)
                                ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGt                                                                      >  1:6132411/1‑101 (MQ=255)
                                 ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTc                                                                     <  2:9087286/101‑1 (MQ=255)
                                 ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTc                                                                     >  1:5065416/1‑101 (MQ=255)
                                 ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTc                                                                     >  1:5755314/1‑101 (MQ=255)
                                  tCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCg                                                                    <  2:4092226/101‑1 (MQ=255)
                                    aaTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCt                                                                  >  1:1419674/1‑101 (MQ=255)
                                        gATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTcccc                                                              >  2:3692275/1‑101 (MQ=255)
                                         atatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTccccc                                                             <  1:1318630/101‑1 (MQ=255)
                                         atatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTccccc                                                             >  2:8650405/1‑101 (MQ=255)
                                          tatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTcccccc                                                            <  1:558857/101‑1 (MQ=255)
                                          tatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTcccccc                                                            >  2:2695185/1‑101 (MQ=255)
                                              gCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAC.CCGCCC.CGCACCACCGGTCGCTCCCCCCGCgg                                                        >  1:6928219/1‑101 (MQ=255)
                                                aaaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCgggg                                                      >  1:6690611/1‑101 (MQ=255)
                                                   aCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCg                                                   <  1:8165115/101‑1 (MQ=255)
                                                    ccGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGt                                                  <  1:1365291/101‑1 (MQ=255)
                                                      gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCCCCGGTCGCTCCCCCCGCGGGGGCGCgg                                                >  2:8958411/1‑101 (MQ=255)
                                                       ccgataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTg                                                 <  2:4222500/91‑1 (MQ=0)
                                                        ccGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                              <  1:654882/101‑1 (MQ=255)
                                                         gataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGa                                               <  2:7471257/93‑1 (MQ=0)
                                                         gataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAACCCGCAA.TCGCCCGGGCTCGATC.GCCGCTCCCCCCCCCGGGGCGTGGa                                               >  1:4263691/9‑101 (MQ=0)
                                                          gcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                            >  2:3611573/1‑101 (MQ=255)
                                                          gcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                            >  1:2533394/1‑101 (MQ=255)
                                                            gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  1:180610/101‑1 (MQ=255)
                                                             aCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         <  1:1015678/101‑1 (MQ=255)
                                                              cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaac                                        <  1:2346627/101‑1 (MQ=255)
                                                               aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaaca                                       >  2:4749135/1‑101 (MQ=255)
                                                                tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaacac                                      >  2:1853175/1‑100 (MQ=255)
                                                                tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaacaa                                      <  1:4775690/101‑1 (MQ=255)
                                                                cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaac                                        >  1:449326/2‑101 (MQ=2)
                                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAccc                                   <  2:665072/101‑1 (MQ=255)
                                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAccc                                   <  2:5970668/101‑1 (MQ=255)
                                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtg                                    >  1:1146907/1‑97 (MQ=2)
                                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtg                                    >  2:802275/1‑97 (MQ=2)
                                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAcccag                                 >  2:8331490/1‑99 (MQ=255)
                                                                      tCCCCCCGCGGGGGCGTTGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtc                                  >  1:6235966/1‑95 (MQ=2)
                                                                         ccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccag                               <  2:2385880/101‑10 (MQ=2)
                                                                         ccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                             >  2:7323479/1‑101 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                             <  2:4938549/101‑1 (MQ=255)
                                                                          cccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccagc                              <  2:3887612/101‑11 (MQ=2)
                                                                          cccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATg                            <  2:6478676/101‑1 (MQ=255)
                                                                          accGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATg                            <  1:1663417/100‑1 (MQ=255)
                                                                             gcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGcc                         <  2:6160776/101‑1 (MQ=255)
                                                                              cgGGGGCGTGGATCGAAACACCG.TCATC.CCCACGCAC.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                        <  2:3518349/101‑1 (MQ=255)
                                                                              cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                        <  2:7820274/101‑1 (MQ=255)
                                                                              cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                        >  1:28899/1‑101 (MQ=255)
                                                                               gggggCGTGGATCGAAACACCG.TCATC.ACCACTCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCg                       <  1:4671463/101‑1 (MQ=255)
                                                                                 gggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGcc                     <  1:8581522/101‑1 (MQ=255)
                                                                                 gggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGcc                     >  1:35643/1‑101 (MQ=255)
                                                                                  ggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                    >  2:5793801/1‑101 (MQ=255)
                                                                                   gCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTc                   <  2:553449/101‑1 (MQ=255)
                                                                                    cGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                  <  2:4410502/101‑1 (MQ=255)
                                                                                     gTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAg                 >  2:529179/1‑101 (MQ=255)
                                                                                      tGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgc                <  2:139668/101‑1 (MQ=255)
                                                                                       ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcg               <  1:7086057/101‑1 (MQ=255)
                                                                                       ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcg               >  1:1500884/1‑101 (MQ=255)
                                                                                          tccaatcacCG.TCAGC.CCCACGCAC.CCGCCC.TGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccg            <  2:3218783/95‑1 (MQ=25)
                                                                                            gAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCcgcg          >  2:5645894/1‑101 (MQ=255)
                                                                                              aaCACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCt        <  2:4598236/101‑1 (MQ=255)
                                                                                              aaCACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCt        >  1:2724713/1‑101 (MQ=255)
                                                                                               acacCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCCCAGCGCCgcgccg       >  2:2778097/1‑99 (MQ=255)
                                                                                                cacCG.GCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTgc      >  1:8845799/1‑100 (MQ=255)
                                                                                                 cccG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGAc     <  2:163760/100‑1 (MQ=255)
                                                                                                 acCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGAc     <  2:1193589/101‑1 (MQ=255)
                                                                                                 acCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGAc     <  1:8238073/101‑1 (MQ=255)
                                                                                                 acCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGAc     <  1:8704628/101‑1 (MQ=255)
                                                                                                    g.TCATC.CCCACGCAA.CCGCCG.TGCACCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTc  <  2:8478439/101‑1 (MQ=255)
                                                                                                     |                                                                                                       
TCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTC  >  NC_014034/1397369‑1397569

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG