Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,640,006 +C 2.3% intergenic (+86/‑59) mccB → / → mccA methylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,640,0062.→C 2.3% 2.9 ‑4.5 0.0209intergenic (+86/‑59)mccB/mccAmethylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha
Reads supporting (aligned to +/- strand):  new base (1/2):  ref base (193/11):  total (194/15)
Fisher's exact test strand distribution p-value = 1.06e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 296 total aligned reads displayed.

ATCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGG....G.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGCCAACAGGGCCGAGATCGCC  >  NC_014034/1639906‑1640106
                                                                                                               |                                                                                                             
 tCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGGTATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTTCCCCCAAAAT.CCC.GGGGGGCC..Ccggg....gg                                                                                                               >  1:4270334/1‑100 (MQ=37)
 tCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..Cgggg....gg                                                                                                               <  2:3728467/101‑2 (MQ=255)
  cTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..Cgggg....ggc                                                                                                              <  1:3231846/101‑3 (MQ=255)
   ttCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTTGGGG.AAGATGAAACCCATCCCTTTCCTTCTTTCCCCAAAATC.CCC.GGGGGGCC..Ggggg....ggg                                                                                                              >  1:4563851/1‑99 (MQ=25)
   ttCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..Cgggg....ggca                                                                                                             <  1:4901576/101‑4 (MQ=255)
    tCCGGATGTGACTTTCGATGCCTCCGGCGGGGGGATTTTGGGG.AAGAAGAAAACCAACCCCTT.CATCTTTCCCCCAATAT.CCC.CGGGGGCC..Cgggg....ggggg                                                                                                            >  1:171706/1‑97 (MQ=255)
    tCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..Cgggg....ggcag                                                                                                            <  2:2886106/101‑5 (MQ=255)
      cGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGG.CAGATGAAACCCCTCCCTTT.CCTCTTGTCCCCAATAT.CCCGGGGGGGCC..Cgggg....ggcgtg                                                                                                           >  2:66026/1‑96 (MQ=25)
       ggATGTGACTTTCGATGCCTCCGGCGGGGGTATTTTGGGG.AATATGAAAACCATCCCTTT.CATCTTTCCCCAAATATCCCC.GGGGGGCC..GGGGG....g.....c..ggggc                                                                                                   >  2:4363115/1‑97 (MQ=25)
       ggATGTGACTTTCGATGCCTCCGGCGGGGGTATTTGGGGC.AAGATGAAACCCATCCCCTT.CATCTTTCCCCCAATAT.CCC.GGGGGGCC..Cgggg....ggcagcgc                                                                                                         >  1:8627627/1‑94 (MQ=37)
         aTGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGG.AAGATGAAACCCATCCCTTT.CATCTTTCCCCAAATAT.CCC.CGGGGGCC..CGGGG....G........G...ccccgccc                                                                                            >  1:2832102/1‑97 (MQ=25)
          tgtgACTTTCGATGCCTCCGGCGGGGATATTTGGGGG.AAGATGAAAACCACCCCCTT.CATCTTTCCCCCAATGA.CCC.CGGGGGCC..Cgggg....ggcggggcccc                                                                                                      >  1:6393049/1‑91 (MQ=21)
                        ccTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGcc                                                                                  >  2:6933723/1‑101 (MQ=21)
                          cccGGGGGGGATTTTGGGGGA.AAGATAACCCCCCTCCTTTC.CCTTTTCCCCCAAATAC.CCG.GGGGGGCC..GGGGG....GGC...C..GCC.CCCCCCGCCACTTCCGCCAc                                                                                <  1:6695880/100‑1 (MQ=255)
                              ggggggATTTTGGGGGCAAAGATAAACCCCCCCCCTTT.CTTTTTCCCCAAAATGC.CCG.GGGGGGCG..GGGGG....G.....CCGGCC.CCCCCCGCCACTTCCGCCACggg                                                                             <  2:142251/99‑1 (MQ=255)
                               cggGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGc                                                                           >  1:5299640/1‑101 (MQ=25)
                               cggGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGc                                                                           >  2:1532628/1‑101 (MQ=21)
                               cggGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGc                                                                           >  1:7364468/1‑101 (MQ=25)
                               cggGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGc                                                                           >  1:7421925/1‑101 (MQ=25)
                                ggggATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCa                                                                          >  2:6307684/1‑101 (MQ=25)
                                ggggATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCa                                                                          >  2:6194160/1‑101 (MQ=25)
                                 gggATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAg                                                                         >  2:7546195/1‑101 (MQ=25)
                                 gggATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAg                                                                         >  2:5366609/1‑101 (MQ=25)
                                 gggATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAg                                                                         >  1:5095382/1‑101 (MQ=25)
                                   gATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAgcg                                                                       >  1:4737266/1‑101 (MQ=25)
                                   gATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAgcg                                                                       >  2:9027941/1‑101 (MQ=25)
                                   gATATTGGGGGC.AAGATGAACCCCACCCTTTT.CATCTGCCCCAAAATAC.CCG.GGGGGGCCG.GGGGG....G.....C..GGCCCCCCCCGCCACTTCCGCCACGGGGCAgcg                                                                       <  2:4495633/101‑1 (MQ=255)
                                     tatTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGcc                                                                     >  1:6583268/1‑101 (MQ=25)
                                      tttgggggC.AGGAGCAACCCCTCCCCTTT.CCTCTT.CCCCAAAACC.CCC.GGGGGGCC..CGGGG....GGC...C..GGCCCCCCCCGCCACTTCCGCCACGGGGCAGCGccc                                                                    <  1:619589/97‑1 (MQ=9)
                                      atTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGccc                                                                    >  1:1321019/1‑101 (MQ=25)
                                      atTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGccc                                                                    >  2:4749591/1‑101 (MQ=25)
                                       tttGGGGG.AAAAATAACCCCCCCCCTTTCCATCTCCCCCCAAATCC.CGG.GGGGGGCCG.GGGGG....G.....C..GCCCCCCCCCGCCACTTCCGCCACGGGGCAGCGccc                                                                    <  1:7967255/101‑1 (MQ=255)
                                       tttGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGcccc                                                                   >  1:7554290/1‑101 (MQ=25)
                                        ttGGGGG.AAAAAGAACCCCCTTCCTTT.CCTTTTCCCCCAAAATC.CCC.GGGGGGCC..CGGGG....GGC...C..GCCCCCCCCCGCCACTTCCGCCACGGGGCAGCGcccc                                                                   <  1:2060102/101‑1 (MQ=255)
                                        tgggggC.AAGAGGAACCCCACCCTTTT.CATCTGCCCCAAAATCC.CCG.GGGGGGCCG.GGGGG....G.....C..GGCCCCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCg                                                                  <  1:9191086/99‑1 (MQ=255)
                                         tGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCgg                                                                 >  1:1424344/1‑101 (MQ=25)
                                         tGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCgg                                                                 >  1:7116763/1‑101 (MQ=25)
                                          ggggC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGc                                                                >  2:7249915/1‑101 (MQ=25)
                                           gggC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGcc                                                               >  1:9037053/1‑101 (MQ=25)
                                            ggC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCg                                                              >  1:2407970/1‑101 (MQ=25)
                                             gC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGt                                                             >  2:8477234/1‑101 (MQ=25)
                                              c.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGtt                                                            >  2:3299270/1‑101 (MQ=25)
                                              c.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGtt                                                            >  2:2243376/1‑101 (MQ=25)
                                              c.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGtt                                                            >  1:4005176/1‑101 (MQ=25)
                                              c.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGtt                                                            >  2:1039435/1‑101 (MQ=25)
                                                aaGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTg                                                           >  1:3865379/1‑101 (MQ=25)
                                                aaGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTg                                                           >  2:3591808/1‑101 (MQ=25)
                                                 aGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGa                                                          >  2:7382128/1‑101 (MQ=25)
                                                 aGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGa                                                          >  1:6840855/1‑101 (MQ=25)
                                                  gaagAAACCCCTCCCTTT.CCTTCTTCCCCAAAATC.CCC.GGGGGGCC..CGGGG....GGGCCCC..C...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGa                                                          <  2:5484338/98‑1 (MQ=255)
                                                   aTGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACg                                                        >  2:4622447/1‑101 (MQ=25)
                                                   aTGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACg                                                        >  1:2192256/1‑101 (MQ=25)
                                                    tGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGGAGCGCCCCGGCCGTTGACGa                                                       >  1:8825001/1‑101 (MQ=21)
                                                    tGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGa                                                       >  2:4666865/1‑101 (MQ=25)
                                                    tGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGa                                                       >  1:7344826/1‑101 (MQ=25)
                                                     gAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGaa                                                      >  1:1135490/1‑101 (MQ=25)
                                                      aaaCCCCTCCCTTC.CCTCTTGCCCCAAATGC.CCC.GGGGGGCCGGGGGGG....G.....C..GCCCCCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGaa                                                      <  2:6651287/101‑1 (MQ=255)
                                                      aaaCCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAg                                                     >  2:1860851/1‑101 (MQ=25)
                                                      aaaCCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAg                                                     >  2:2558581/1‑101 (MQ=25)
                                                       aaCCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAgg                                                    >  2:5143506/1‑101 (MQ=25)
                                                       aaCCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAgg                                                    >  2:8229702/1‑101 (MQ=25)
                                                        aCCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACNANGga                                                   >  1:4148239/1‑101 (MQ=21)
                                                         cccATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGgag                                                  >  1:5736475/1‑101 (MQ=25)
                                                          ccATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGgaga                                                 >  2:3410930/1‑101 (MQ=25)
                                                           cATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGAc                                                >  2:5787473/1‑101 (MQ=25)
                                                            aTCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGAcc                                               >  2:8063806/1‑101 (MQ=21)
                                                             tCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACcg                                              >  1:1923666/1‑101 (MQ=25)
                                                              cccTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGGCCCGGCCGTTGACGAAGGAGGCcgc                                             >  2:6514684/1‑101 (MQ=21)
                                                                cTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGa                                           >  2:2473384/1‑101 (MQ=25)
                                                                cTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGa                                           >  2:5233704/1‑101 (MQ=25)
                                                                cTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGa                                           >  1:7849319/1‑101 (MQ=25)
                                                                 ttt.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGAt                                          >  2:4330639/1‑101 (MQ=25)
                                                                 ttt.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGAt                                          >  2:787272/1‑101 (MQ=25)
                                                                  tt.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATg                                         >  2:6479651/1‑101 (MQ=25)
                                                                  tt.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATg                                         >  1:1038152/1‑101 (MQ=25)
                                                                  tt.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATg                                         >  1:5951726/1‑101 (MQ=25)
                                                                  tt.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATg                                         >  2:3006380/1‑101 (MQ=25)
                                                                  tt.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATg                                         >  1:8543598/1‑101 (MQ=25)
                                                                  tt.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATg                                         >  1:8325347/1‑101 (MQ=25)
                                                                   t.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGt                                        >  1:8257205/1‑101 (MQ=25)
                                                                     cATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGtt                                       >  1:3498126/1‑101 (MQ=25)
                                                                     cATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGtt                                       >  2:6062034/1‑101 (MQ=25)
                                                                     cATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGtt                                       >  2:7280503/1‑101 (MQ=25)
                                                                         ttGCCCCAAATAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCaa                                   >  1:3581661/1‑101 (MQ=25)
                                                                                 aaTAT.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCt                           >  1:288661/1‑101 (MQ=25)
                                                                                  atat.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTg                          >  2:4949334/1‑101 (MQ=37)
                                                                                  atat.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTg                          >  2:2784791/1‑101 (MQ=37)
                                                                                   tat.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGa                         >  2:9086589/1‑101 (MQ=37)
                                                                                    at.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATcccagc                        >  2:4189053/1‑97 (MQ=25)
                                                                                    at.CCC.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGAt                        >  2:6823338/1‑101 (MQ=37)
                                                                                       ccc.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCg                      >  2:6096267/1‑101 (MQ=37)
                                                                                        cc.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGc                     >  2:8870818/1‑101 (MQ=37)
                                                                                         c.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGcc                    >  2:3123419/1‑101 (MQ=37)
                                                                                         c.GGGGGGCC..CGGGGGGCAG.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGcc                    >  2:5697603/1‑101 (MQ=37)
                                                                                                    ccggggggcag.....c..g...cCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGCCAACAGGGc            >  2:2067820/11‑101 (MQ=255)
                                                                                                      ggggggcag.....c..g...cCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGCCAACAGGGCCg          >  2:363259/9‑101 (MQ=255)
                                                                                                       gggggcag.....c..g...cCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGCCAACAGGGCCga         >  1:5511059/8‑101 (MQ=255)
                                                                                                        ggggcag.....c..g...cCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGCCAACAGGGCCgag        >  1:6809132/7‑101 (MQ=255)
                                                                                                          ggcag.....c..g...cCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGCCAACAGGGCCGAGAt      >  2:3893010/5‑101 (MQ=255)
                                                                                                           gcag.....c..g...cCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGCCAACAGGGCCGAGATc     >  1:7663735/4‑101 (MQ=255)
                                                                                                               |                                                                                                             
ATCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATCTTGCCCCAAATAT.CCC.GGGGGGCC..CGGGG....G.....C..G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGCCAACAGGGCCGAGATCGCC  >  NC_014034/1639906‑1640106

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG