BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,732,695	+C	1.6%	intergenic (‑119/‑284)	RCAP_rcc01601 ← / → tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,732,695	1	.→C	1.6%	3.0 ‑0.6 0.0	245	intergenic (‑119/‑284)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand): new base (0/4): ref base (63/178): total (63/182)
Fisher's exact test strand distribution p-value = 5.75e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.


CCTAGAGTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.CC..GGGC...GGG..CGG..G..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTCC  >  NC_014034/1732595‑1732784


ccTAGAGTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCgcggc.g                                                                                                        <  2:248615/101‑1 (MQ=255)


 cTAGAGTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.a                                                                                                      >  1:9845/1‑101 (MQ=255)


 cTAGAGTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.a                                                                                                      >  1:5164153/1‑101 (MQ=255)


  tAGAGTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCTAGGGTTTCGCCGCGGCGGGGCCgcggc.gc                                                                                                       >  1:8942705/1‑100 (MQ=255)


    gagTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.ccc                                                                                                  <  2:8652444/101‑2 (MQ=255)


     agTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.G.cccg                                                                                                 >  1:8624590/1‑99 (MQ=255)


     agTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.cccg                                                                                                 <  1:7973257/101‑3 (MQ=255)


      gTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.Cc..cgg                                                                                              >  1:1643816/1‑101 (MQ=255)


      gTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.Cc..cgg                                                                                              >  1:8530742/1‑101 (MQ=255)


       tttATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.G.Cc..cggg                                                                                             >  2:1763932/1‑100 (MQ=255)


        ttATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.Cc..cgggg                                                                                            >  2:1295601/1‑99 (MQ=255)


        ttATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.Cc..cgggc                                                                                            >  2:5100299/1‑99 (MQ=255)


        ttATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCCCGGCGGGGCCCCGGC.G.A.Cc..cgggg                                                                                            >  1:223/1‑99 (MQ=255)


         tatataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGG.G.ACCc..gggc...g                                                                                         >  2:8808093/1‑101 (MQ=255)


         tatataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCc..gggc...g                                                                                         >  1:6901393/1‑101 (MQ=255)


          atataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.Cc..cggg...ggg                                                                                       >  1:2331098/1‑101 (MQ=255)


          atataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.Cc..cggg...ggg                                                                                       >  1:2717092/1‑101 (MQ=255)


          atataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.Cc..cggg...ggg                                                                                       >  1:917195/1‑101 (MQ=255)


           tataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGTCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.Cc..cggg...gggg                                                                                      >  1:7329098/1‑100 (MQ=255)


           tataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCc..gggc...ggg                                                                                       >  2:2016100/1‑101 (MQ=255)


           tataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.Cc..cggg...gggg                                                                                      >  2:3927475/1‑100 (MQ=255)


            ataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCc..gggc...gggg                                                                                      >  2:2724685/1‑100 (MQ=255)


            ataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCc..gggc...gggg                                                                                      <  2:6901108/101‑2 (MQ=255)


            ataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.Cc..cggggcggg                                                                                        >  2:7885396/1‑95 (MQ=255)


            ataGGGTATCGGGGGGAGAGGTAAAAGCCGTCGGTGGGGGGCGGAAAGCCGCGGGCCAAGC.AGGGTTTCGGCGCAGCGGGGCCGCGGC.G.AGGc..gggc...gggg                                                                                      >  2:1283380/1‑100 (MQ=255)


             taGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCc..gggc...ggggg                                                                                     <  2:5726826/101‑3 (MQ=255)


              aGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGCTTCGCCGCGGCGGGGCCGCGGC.G.A.Cc..cggc...gcggggc                                                                                   >  1:9001388/1‑95 (MQ=255)


               gggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGG.GCC.Cc..gggc...gggggcg                                                                                   >  2:4210820/1‑97 (MQ=255)


               gggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCc..gggc...gggggct                                                                                   <  2:5247596/101‑5 (MQ=255)


               gggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCc..gggc...ggg..ggg..g                                                                               >  2:7064875/1‑101 (MQ=255)


                ggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCc..gggc...gggggctc                                                                                  <  2:2538498/101‑6 (MQ=255)


                ggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCc..gggc...gggggctc                                                                                  <  2:8903601/101‑6 (MQ=255)


                 gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCc..gggc...gggggctcc                                                                                 <  1:1511753/101‑7 (MQ=255)


                 gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGC...GGG...GG..G..Gcc                                                                          >  1:1158653/1‑101 (MQ=255)


                  tATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGCGG.C.CC..GGGC...GGG..GGG..C..Gcg                                                                          >  2:6414545/1‑100 (MQ=255)


                  tATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGC...GGG...GG..G..Gccg                                                                         >  1:3569021/1‑100 (MQ=255)


                   aTCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCAGCGTGCCAAGC.AGGGTTTCGACGCGGCGGGGACGCAGG.G.A.CC..CGGT...CGG..GGG..G..Gccaa                                                                        >  2:4884481/1‑99 (MQ=255)


                   aTCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGAGGAAAGCCGCGTGCCAAGA.CGGGTTTCGCCGCGGCGGGGAGGCGGC.G.AGCc..cggc...ggg..ggg..gcgcg                                                                           >  2:2932055/1‑97 (MQ=255)


                     cGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGC...AGG...GG..G..Gccggcc                                                                      >  2:832119/1‑97 (MQ=255)


                     cGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.CC..CGGG...GGG..GGG..G..Gccgccc                                                                      >  2:2328319/1‑100 (MQ=255)


                      ggggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGC...GGG..Ggg..g..gggcccc                                                                      >  1:5937719/1‑95 (MQ=255)


                       gggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGG.G.ACCC..GGGC...GGG..GGG..G..Cccccccc                                                                     >  2:7477722/1‑99 (MQ=255)


                       gggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGC...GGG..GGG..G..Gccccccc                                                                     >  1:3758628/1‑99 (MQ=255)


                       gggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.CCCGGGGC...GGG..Ggg..g..gggcccc                                                                      >  1:3523465/1‑95 (MQ=255)


                        ggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.CC..CCGG...GGG..GGG..G..Gccccccccc                                                                   >  1:2540196/1‑97 (MQ=255)


                          ggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGC...GGG..Ggg..g..ggcgccccccc                                                                  >  2:3222525/1‑91 (MQ=255)


                             gagGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGC...GGG...GG..G..Gcgccctccccgccc                                                              >  1:6728059/1‑92 (MQ=255)


                              agGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCAcccc                                                                <  1:5219512/101‑1 (MQ=25)


                              agGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCAcccc                                                                <  1:1938919/101‑1 (MQ=25)


                              agGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGC...GGG..GGG..G..CCGCCCCCCcccccc                                                              >  2:5470271/1‑100 (MQ=255)


                               ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCAccccc                                                               <  1:5750371/101‑1 (MQ=25)


                               ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCAccccc                                                               <  1:7279045/101‑1 (MQ=25)


                                gTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGC...GGG..GGG..GCCGCCCCCGCCCCACCg                                                              >  2:6015676/1‑101 (MQ=255)


                                 tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCgg                                                             <  2:4827655/101‑1 (MQ=25)


                                  aaaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGc                                                            <  1:3927590/101‑1 (MQ=25)


                                  aaaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGc                                                            <  1:2103293/101‑1 (MQ=25)


                                    aGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGC...GGG..GGG..T..CCGCCCCCCccccccccgcgg                                                        >  1:4004202/1‑94 (MQ=25)


                                     ggCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTa                                                         <  1:3049472/101‑1 (MQ=25)


                                      gCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTAt                                                        <  1:5590641/101‑1 (MQ=25)


                                        cGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTAttt                                                      <  2:2372562/101‑1 (MQ=25)


                                         ggcGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTg                                                     <  2:7322606/99‑1 (MQ=21)


                                           cGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTgcg                                                   <  2:3550895/101‑1 (MQ=21)


                                            ggTGCGGGTCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTgcgc                                                  <  2:4531223/101‑1 (MQ=21)


                                            ggTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTgcgc                                                  <  1:3214200/101‑1 (MQ=25)


                                             gTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGcc                                                 <  1:1786473/101‑1 (MQ=21)


                                              tGCGGGGCGGAAAGCCGNGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCa                                                <  2:3758868/101‑1 (MQ=21)


                                                cGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCaag                                              <  2:9051217/101‑1 (MQ=21)


                                                cGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCaag                                              <  2:8942284/101‑1 (MQ=21)


                                                      ggAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGaaa                                        <  2:1643769/101‑1 (MQ=18)


                                                        aaaGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACa                                      <  1:313337/101‑1 (MQ=18)


                                                         aaGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACaa                                     <  2:8530357/101‑1 (MQ=18)


                                                         aaGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACaa                                     <  2:8220967/101‑1 (MQ=18)


                                                          aGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAg                                    <  2:4182555/101‑1 (MQ=18)


                                                           gCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCTCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGc                                   <  1:8161434/101‑1 (MQ=18)


                                                           gCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGc                                   <  1:7696852/101‑1 (MQ=18)


                                                               cgTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGC...GGG..GGG..GCCCCCCCCCCACCCCCCGCGGATTTTCGCCCAGAAGAAAAAAGCgggc                               >  2:3344069/1‑100 (MQ=255)


                                                                gTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGAc                              <  2:2819354/101‑1 (MQ=18)


                                                                  gCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCa                            <  1:6039709/101‑1 (MQ=18)


                                                                   ccAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAg                           <  2:771328/101‑1 (MQ=18)


                                                                    cAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGC...GGG..GGGTCC..CCCCCCCCCCCCCCCGCGTCTTTTCGCCCAGAAGAAAAAAGCcggggccgg                          >  2:8550346/1‑95 (MQ=255)


                                                                       gC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..TCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGccc                       <  2:3719132/101‑1 (MQ=18)


                                                                          aGGGTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGccccc                     <  2:926006/101‑1 (MQ=18)


                                                                           gggTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGcccccc                    <  1:1221964/101‑1 (MQ=18)


                                                                            ggTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCt                   <  1:8274933/101‑1 (MQ=18)


                                                                            ggTTTCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCt                   <  1:4803202/101‑1 (MQ=18)


                                                                              tttCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGc                 <  1:2493419/101‑1 (MQ=18)


                                                                               ttCGCCGCGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..CCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCa                <  2:9000966/101‑1 (MQ=25)


                                                                                tcgcgcgggcggGGCCGCGGCGG.C.CC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCACTGCAg               <  1:2932153/94‑1 (MQ=12)


                                                                                 cgccgcGGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGc              <  1:5679457/101‑1 (MQ=18)


                                                                                    cgcgGGGGGGCCGCGGC.G.ACCC..GGGC...GGGGCCTC..C..GCCCCCGCACTCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGc           <  1:1395764/101‑1 (MQ=25)


                                                                                    cgcgGCGGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGc           <  1:9051967/101‑1 (MQ=18)


                                                                                    cgcgGCGGGGCCGCGGC.G.A.CC..AGGCCGGGGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCAAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGc           <  2:6731655/101‑1 (MQ=18)


                                                                                     gcggcgGGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCa          <  1:4379799/101‑1 (MQ=18)


                                                                                      cggcggGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCAt         <  2:3088837/101‑1 (MQ=18)


                                                                                      cggcggGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCAt         <  1:4615371/101‑1 (MQ=18)


                                                                                        gcggGGCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGc       <  2:4421199/101‑1 (MQ=18)


                                                                                          ggggCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCCg     <  1:6344240/101‑1 (MQ=18)


                                                                                          ggggCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCCGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCCGCAGCCGCACGccc     >  2:1064201/1‑100 (MQ=18)


                                                                                            ggCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTc   <  2:5356495/101‑1 (MQ=18)


                                                                                             gCCGCGGC.G.ACCC..GGGCGG.GGG..CTC..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTcc  <  1:7573093/101‑1 (MQ=18)


CCTAGAGTTTATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGC.AGGGTTTCGCCGCGGCGGGGCCGCGGC.G.A.CC..GGGC...GGG..CGG..G..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTCC  >  NC_014034/1732595‑1732784

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 39 ≤


ATCG