Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,732,702 +GG 100% intergenic (‑126/‑277) RCAP_rcc01601 ← / → tgt FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,732,7021.→G100.0% 27.8 169intergenic (‑126/‑277)RCAP_rcc01601/tgtFeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (2/154):  ref base (9/4):  total (11/158)
Fisher's exact test strand distribution p-value = 1.51e-10
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.59e-07
*NC_0140341,732,7022.→G100.0% 27.8 170intergenic (‑126/‑277)RCAP_rcc01601/tgtFeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (2/155):  ref base (9/4):  total (11/159)
Fisher's exact test strand distribution p-value = 1.43e-10
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.12e-07

Only 100 of 258 total aligned reads displayed.

TATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.A.CC..GGGC..GGG..CGG..G...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTCC  >  NC_014034/1732604‑1732784
                                                                                                       ||                                                                                         
tatataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGGG.ACCc..gggc..g                                                                                          >  2:8808093/1‑101 (MQ=255)
 atataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.A.Cc..cggg..ggg                                                                                        >  1:2331098/1‑101 (MQ=255)
 atataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.A.Cc..cggg..ggg                                                                                        >  1:917195/1‑101 (MQ=255)
 atataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.A.Cc..cggg..ggg                                                                                        >  1:2717092/1‑101 (MQ=255)
  tataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGTCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.A.Cc..cggg..gggg                                                                                       >  1:7329098/1‑100 (MQ=255)
  tataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGTCGCGGCGGGGCCGCGGCG.ACCc..gggc..ggc                                                                                        >  1:4978701/1‑100 (MQ=255)
   ataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCc..gggc..gggg                                                                                       >  2:4726699/1‑100 (MQ=255)
   ataGGGTATCGGGGGGAGAGGTAAAAGCCGTCGGTGGGGGGCGGAAAGCCGCGGGCCAAGCAGGGTTTCGGCGCAGCGGGGCCGCGGCG.AGGc..gggc..gggg                                                                                       >  2:1283380/1‑100 (MQ=255)
    taGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCc..gggc..ggggg                                                                                      >  1:4791151/1‑99 (MQ=255)
    taGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCc..gggc..ggggg                                                                                      <  2:5726826/101‑3 (MQ=255)
     aGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGGG.A.Cc..cggg..ggg..ggg..g                                                                                >  1:6668487/1‑101 (MQ=255)
      gggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGGGCC.Cc..gggc..gggggcg                                                                                    >  2:4210820/1‑97 (MQ=255)
      gggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCc..gggc..gggggct                                                                                    <  2:5247596/101‑5 (MQ=255)
      gggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCc..gggc..ggg..ggg..g                                                                                >  2:3675076/1‑101 (MQ=255)
      gggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCc..gggc..ggg..ggg..g                                                                                >  2:7064875/1‑101 (MQ=255)
       ggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCc..gggc..gggggctc                                                                                   <  2:2538498/101‑6 (MQ=255)
       ggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCc..gggc..gggggctc                                                                                   <  2:8903601/101‑6 (MQ=255)
        gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCc..gggc..gggggctcc                                                                                  <  2:4146603/101‑7 (MQ=255)
        gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGC..GGG...GG..G...Gcc                                                                          >  1:1158653/1‑101 (MQ=255)
         caTCGGGGGGAGAGGTAAAGGCCGTCGGTGCGCGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCGCC.Cc..gggc..gggggctccg                                                                                 <  2:2888033/100‑8 (MQ=255)
          aTCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGC..GGG...GG..G...Gcccc                                                                        >  2:1612037/1‑101 (MQ=255)
          aTCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.A.CC..CGGG..GGG..Ggg..g...ggcgc                                                                        >  1:2528202/1‑97 (MQ=255)
            cGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGG..GGG..GGG..G...Gcgccc                                                                       >  1:6770678/1‑101 (MQ=255)
            cGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGC..AGG...GG..G...Gccggcc                                                                      >  2:832119/1‑97 (MQ=255)
            cGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.A.CC..CGGG..GGG..GGG..G...Gccgccc                                                                      >  2:2328319/1‑100 (MQ=255)
            cGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.A.CC..CGGG..GGG..GGG..G...Gcacccc                                                                      >  2:4858018/1‑100 (MQ=255)
             ggggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGC..GGG..Ggg..g...gggcccc                                                                      >  1:5937719/1‑95 (MQ=255)
             ggggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.A.Cc..cggg..ggg..gggcgggggccc                                                                          >  1:5714952/1‑92 (MQ=255)
             ggggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.A.CC..CGGG..GGG..GGG..G...Gccccccc                                                                     >  1:9004246/1‑99 (MQ=255)
              gggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGGG.ACCC..GGGC..GGG..GGG..G...Cccccccc                                                                     >  2:7477722/1‑99 (MQ=255)
              gggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGC..GGG..GGG..G...Gccccccc                                                                     >  1:4534886/1‑99 (MQ=255)
              gggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.A.CCCGGGGC..GGG..Ggg..g...gggcccc                                                                      >  1:3523465/1‑95 (MQ=255)
               ggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.A.CC..CCGC..GCG..Ggg..g...gggcggcgcc                                                                   >  2:1758959/1‑92 (MQ=255)
                 ggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGC..GGG..Ggg..g...ggcgccccccc                                                                  >  2:3222525/1‑91 (MQ=255)
                    gagGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGC..GGG..GGC..G...GCCGCCCCCccccc                                                               >  1:2308456/1‑101 (MQ=255)
                     agGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCAcccc                                                                <  1:1938919/101‑1 (MQ=25)
                      ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCAccccc                                                               <  1:7279045/101‑1 (MQ=25)
                      ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCAccccc                                                               <  1:3567784/101‑1 (MQ=25)
                      ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCAccccc                                                               <  1:5750371/101‑1 (MQ=25)
                         aaaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGc                                                            <  1:2103293/101‑1 (MQ=25)
                         aaaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGC..GGG..GGGTCC...GGCCCCGCACCCCCGGc                                                            >  2:4200446/1‑101 (MQ=255)
                            ggCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTa                                                         <  1:3049472/101‑1 (MQ=25)
                             gCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTAt                                                        <  1:5192050/101‑1 (MQ=25)
                             gCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTAt                                                        <  1:5592399/101‑1 (MQ=25)
                             gCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTAt                                                        <  1:7183287/101‑1 (MQ=25)
                              gatctggTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTAtt                                                       <  2:595651/96‑1 (MQ=21)
                               cGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTAttt                                                      <  1:5546090/101‑1 (MQ=25)
                               cGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTAttt                                                      <  2:6263842/101‑1 (MQ=25)
                               cGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTAttt                                                      <  2:8936504/101‑1 (MQ=25)
                                ggcGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTg                                                     <  2:7322606/99‑1 (MQ=21)
                                  cGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTgcg                                                   <  2:3550895/101‑1 (MQ=21)
                                   ggTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTgcgc                                                  <  1:3214200/101‑1 (MQ=25)
                                     ggCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCa                                                <  2:546338/100‑1 (MQ=21)
                                     ggCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCa                                                <  2:2996180/100‑1 (MQ=21)
                                       cGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCaag                                              <  2:9051217/101‑1 (MQ=21)
                                       cGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCaag                                              <  1:3996934/101‑1 (MQ=21)
                                          ggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCaagaag                                           <  2:6668227/101‑1 (MQ=18)
                                           gCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAagaaga                                          <  2:2360154/101‑1 (MQ=18)
                                              gAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAAc                                       <  2:281692/101‑1 (MQ=18)
                                               aaaGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACa                                      <  1:940709/101‑1 (MQ=18)
                                                aaGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACaa                                     <  2:8220967/101‑1 (MQ=18)
                                                    cgcgTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCgg                                 <  1:2893085/101‑1 (MQ=18)
                                                      cgTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGa                               <  1:1661085/101‑1 (MQ=18)
                                                      cgTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGC..GGG..GGG..GCC.CCCCCCCCACCCCCCGCGGATTTTCGCCCAGAAGAAAAAAGCgggc                               >  2:3344069/1‑100 (MQ=255)
                                                        tGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGAcc                             <  1:3151674/101‑1 (MQ=18)
                                                          ccAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAg                           <  2:771328/101‑1 (MQ=18)
                                                          ccAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAg                           <  2:3758519/101‑1 (MQ=18)
                                                          ccAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAg                           <  1:1879932/101‑1 (MQ=18)
                                                           cAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAgg                          <  2:3766147/101‑1 (MQ=18)
                                                           cAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGC..GGG..GGGTCC...CCCCCCCCCCCCCCCGCGTCTTTTCGCCCAGAAGAAAAAAGCcggggccgg                          >  2:8550346/1‑95 (MQ=255)
                                                            aaGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.A.CC..CGGG..GGG..GGG..GTCCCCCCCCCCACCCCCCGCGGATTTGCGCCCAGAAGAAAAAAGCGGGACCCGGc                         >  1:3895827/1‑101 (MQ=255)
                                                              gCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGccc                       <  2:2451446/101‑1 (MQ=18)
                                                               cAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCATGcccc                      <  1:6033480/101‑1 (MQ=18)
                                                               cAGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGcccc                      <  2:4947733/101‑1 (MQ=18)
                                                                aGGGTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGccccc                     <  2:926006/101‑1 (MQ=18)
                                                                 gggTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGcccccc                    <  1:6342982/101‑1 (MQ=18)
                                                                 gggTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGcccccc                    <  1:1221964/101‑1 (MQ=18)
                                                                 gggTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGcccccc                    <  2:6970295/101‑1 (MQ=18)
                                                                  ggTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCt                   <  2:6813355/101‑1 (MQ=18)
                                                                  ggTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCt                   <  1:8274933/101‑1 (MQ=18)
                                                                  ggTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGC..GGG..GGG..GCC.GCCCCCCCCCCCCCCCCGCCGTTTTGCCCAGAAGAAAAAAACGGGGACAGGcgccccc                   >  2:1876990/1‑100 (MQ=255)
                                                                   gTTTCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTg                  <  1:762002/101‑1 (MQ=18)
                                                                    tttCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGc                 <  1:2493419/101‑1 (MQ=18)
                                                                    tttCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGc                 <  1:8059519/101‑1 (MQ=18)
                                                                     ttCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCa                <  1:3675185/101‑1 (MQ=18)
                                                                     ttCGCCGCGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...CCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCa                <  2:9000966/101‑1 (MQ=25)
                                                                       cgccgcGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGc              <  1:8542072/101‑1 (MQ=18)
                                                                        gccgcGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGcc             <  1:7953615/101‑1 (MQ=18)
                                                                        gccgcGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGcc             <  2:8624204/101‑1 (MQ=18)
                                                                         ccgcGGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCg            <  2:2618005/101‑1 (MQ=18)
                                                                          cgcgGGGGGGCCGCGGCG.ACCC..GGGC..GGGGCCTC..C...GCCCCCGCACTCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGc           <  1:1395764/101‑1 (MQ=25)
                                                                          cgcgGCGGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGc           <  1:9051967/101‑1 (MQ=18)
                                                                           gcggcgGGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCa          <  1:4379799/101‑1 (MQ=18)
                                                                             ggcggGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATg        <  2:8387048/101‑1 (MQ=18)
                                                                              gcggGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGc       <  2:4421199/101‑1 (MQ=18)
                                                                               cggGGCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGcc      <  1:8814534/101‑1 (MQ=18)
                                                                                ggggCCGCGGCGCC.CC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCCg     <  2:7940752/101‑1 (MQ=18)
                                                                                ggggCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCCGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCCGCAGCCGCACGccc     >  2:1064201/1‑100 (MQ=18)
                                                                                  ggCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTc   <  2:5356495/101‑1 (MQ=18)
                                                                                   gCCGCGGCG.ACCC..GGGCGGGGG..CTC..C...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTcc  <  1:7573093/101‑1 (MQ=18)
                                                                                                       ||                                                                                         
TATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCG.A.CC..GGGC..GGG..CGG..G...GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTCC  >  NC_014034/1732604‑1732784

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG