Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,732,866 +G 91.5% intergenic (‑290/‑113) RCAP_rcc01601 ← / → tgt FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,732,8663.→G91.5% 15.1 ‑0.5 ‑1.782intergenic (‑290/‑113)RCAP_rcc01601/tgtFeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (10/65):  ref base (0/7):  total (10/72)
Fisher's exact test strand distribution p-value = 5.89e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.96e-02

Only 100 of 116 total aligned reads displayed.

CTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCC....GT...C...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCGGC  >  NC_014034/1732766‑1732948
                                                                                                         |                                                                                              
cTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...cccccc                                                                                                 <  2:3895712/101‑1 (MQ=255)
cTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...cccccc                                                                                                 <  1:2406561/101‑1 (MQ=255)
cTGCAGCCACATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...cccccc                                                                                                 <  2:7595793/101‑1 (MQ=255)
 tGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGCGGGGGAGG...cccccc                                                                                                 >  2:5402430/1‑101 (MQ=255)
  gCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGGGGAG...cccccccc                                                                                               >  2:2483603/1‑99 (MQ=255)
  gCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGGGGAG...CCCCCC....gc                                                                                           >  2:2729958/1‑100 (MQ=255)
   cAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCC....gcg                                                                                          <  1:1814054/101‑3 (MQ=255)
   cAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGGGGAG...ccccccccc                                                                                              >  1:2617009/1‑98 (MQ=255)
   cAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGGGAAG...caccccccc                                                                                              >  2:5632416/1‑98 (MQ=255)
    aGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCC....gcgg                                                                                         >  2:3766251/1‑98 (MQ=255)
       cGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCC....gcgggtc                                                                                      <  1:5551296/101‑7 (MQ=255)
        gCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGGGGAG...gccccccccgggtg                                                                                         >  2:8799210/1‑93 (MQ=255)
        gCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGGGGAG...gccccccccgggcg                                                                                         >  2:5164857/1‑93 (MQ=255)
        gCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGGGGAG...gccccccccgggcg                                                                                         >  1:244060/1‑93 (MQ=255)
        gCATCCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCC....gcgggtcg                                                                                     <  1:1064219/101‑8 (MQ=255)
         cATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGGGGAG...gcccccccggggcgc                                                                                        >  1:3805046/1‑92 (MQ=255)
          aTGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGGGGAG...ccccccccggggggcc                                                                                       >  1:5534488/1‑91 (MQ=255)
          aTGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGGGAAG...gccccccccgggcgcc                                                                                       >  2:5961451/1‑91 (MQ=255)
           tGCCGCCCGCGTCAACAGGCAAGGTCCCCGCTTTTCCTAGGCAAAATCCGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCC....gcgggtcgcct                                                                                  <  2:4000740/101‑11 (MQ=37)
              cGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGGGAAG...CCCCCC....GC...C...GG.ccgcccccg                                                                        >  1:8193809/1‑94 (MQ=255)
                      gcAACAGGAGAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCC....GCGGGT...CG.CCTCGGGCTTGccc                                                                   <  2:6776328/100‑1 (MQ=21)
                         acgggCAGGGCCCCCGCTTTTCCTGGGCAAAATACGCCCTCCTTTCTTTTCGGGGGGGCGGG.GGTTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAg                                                                <  2:571090/98‑1 (MQ=12)
                            ggCAGGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTTTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.g                                                            <  1:4492154/101‑1 (MQ=18)
                            ggCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.g                                                            <  2:8963130/101‑1 (MQ=21)
                            ggCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.g                                                            <  1:7822077/101‑1 (MQ=21)
                            ggCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCC....GCGGGT...CG.CCTCGGGCTTGCCCGAGGC.g                                                            <  2:7740725/101‑1 (MQ=21)
                            gccagggcccccGTTTTCCCTGGGCAAAATCCCCCCTTCTTTCTTTGGGGGGGGGGGGG.GGGGAAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.g                                                            <  2:4614877/93‑1 (MQ=2)
                             gCAAGGGCCCCGCTTTTCCTAGGCAAACTACGCCCTTCTTTCTTTGCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.Ga                                                           <  2:929356/101‑1 (MQ=18)
                             gCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.Ga                                                           <  1:921973/101‑1 (MQ=21)
                              cAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.Gaa                                                          >  2:3870616/1‑101 (MQ=21)
                                aGGGCCGCGCTGTCCCTAGTCAAACTTCGCCCTTCTTTCTTTGCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAg                                                        <  2:2887872/101‑1 (MQ=9)
                                 gggCCCGGTTTTTCCGGGGAAAAGTCCGCCCTTGTTTTTGTGGGGGGGGGGGGG.GGGGGAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGc                                                       <  1:6290766/101‑1 (MQ=2)
                                  gcccccGTTTTTCCTAGGCAAAATCCCCCCTTTTTTCTTTGGGGGGGGGCGGG.GGGGGAAC...CCCCCC....GCGGGT...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCt                                                      <  1:67034/99‑1 (MQ=2)
                                    cacggCTTTTCCGGGCCAAAATGGGCTTTTGTTTCTTGCAGGGGGGGGAGA.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.c                                                   <  1:1283412/97‑1 (MQ=2)
                                     cccGCTTTTCCTAGGCAAAATCCGCCCTTCTTTCTTGTCGGGGGGGGGGG.GGGTGAAC...CCCCCC....GCGGGT...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.cc                                                  <  1:8173736/101‑1 (MQ=18)
                                      ccGCTTTCCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCC....GC...GGGTCG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.ccc                                                 <  2:8669873/101‑1 (MQ=18)
                                       cGCTTTTCCTGGGCAAAATGCGCCCTTCTTTCTTTGCGGGGGGGCGGG.GGGTGAAG...CCCCCC....GC...GGGTCG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.cccc                                                <  2:2508570/101‑1 (MQ=17)
                                         cTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTGGGGGGGGGGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.cccccc                                              <  1:5297489/101‑1 (MQ=18)
                                         cTTTCCCTAGGCAAAATCCCCCCTTTTTTCTTTTGGGGGGGGCGGG.GGGTGACG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.cccccc                                              <  1:5963820/101‑1 (MQ=9)
                                          ttttCCTAGGCACAATTCGCCTTTCTTTCTTTGCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCg                                             <  2:4879547/101‑1 (MQ=17)
                                          ttttCCTAGGCAAAATCCGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCg                                             <  2:4676943/101‑1 (MQ=18)
                                           tttCCTAGGCAAAATCCGCCCTTCTTTCTTTGGGGGGGGGGGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGt                                            <  1:4570822/101‑1 (MQ=17)
                                            ttCCTAGGCAAAATGCGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTg                                           <  2:4103966/101‑1 (MQ=18)
                                            ttCCTAGGCAAAANCCGCCTNNNTTTNTTNNCGGGGGGGCGGG.GGTTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTg                                           <  1:3639305/101‑1 (MQ=17)
                                            tcccTAGGCAAAATCCGCCCTTCTTTCTTTTGGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTg                                           <  2:4534751/99‑1 (MQ=17)
                                             tCCTAGGCAAAATCCGCCCTTTTTTCTTTTGGGGGGGGGGGG.GGGTGAAC...CCCCCCGCG.GG...T...CG.CCTTGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGa                                          <  1:4754998/101‑1 (MQ=12)
                                             tCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGGGGAGGC.CCCCCCG...GG...G...CGCCCCCGGGCTGGCCCCCGGC.GGAAGCTT.ccccccccc                                           >  1:6426769/1‑98 (MQ=255)
                                              ccTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGaa                                         <  2:2580140/101‑1 (MQ=21)
                                               cTAGGCAAAATCCGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGaaa                                        <  2:7003610/101‑1 (MQ=18)
                                                 agtcAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAAc                                      <  2:9143811/98‑1 (MQ=18)
                                                 aGGCAAATTCCCCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGGGAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAAc                                      <  1:1687178/101‑1 (MQ=17)
                                                 aGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAAc                                      <  2:8439840/101‑1 (MQ=21)
                                                  gacAAAAGTCGCCCTTCTTTCTTTGGGGGGGGGCGGG.GGTGGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAAcc                                     <  1:5693992/99‑1 (MQ=9)
                                                   gCAAGATGCCCCCTTCTTTTGTTAGGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCt                                    <  2:7089179/101‑1 (MQ=9)
                                                   gCAAAAGACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGTGGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCt                                    <  1:7302494/101‑1 (MQ=18)
                                                    cAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCtt                                   <  2:5169475/101‑1 (MQ=21)
                                                    cAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGGGAAG...CCCCCCGCG.GG...G...CG.CCTCGGGGCTGGCCGAGGG.GGAAGCTT.CCCCCCCGGGAAAccct                                   >  1:2980902/1‑99 (MQ=12)
                                                      aaaTACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGGGGAGGC.CCCCCCGG..GG...C...GG.CCCCCGCCTTCCCCGGGGG.GAAAGCGT.CCCCCCCGGAAAAccctc                                  >  1:6050026/1‑98 (MQ=255)
                                                       aattccgccTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGcc                                <  1:5888929/94‑1 (MQ=18)
                                                       aaTCCGCCCTTCTTTCTTTGCGGGGGGGGGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGcc                                <  1:2629042/101‑1 (MQ=18)
                                                          aCGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGGGGAGCCCCCCCCC....GG...G...CGCCCCCGGGCTTCCCCGGGGG.GAAGGCTT.CCCCCCGGGAAAACCtgccccc                              >  2:5253443/1‑95 (MQ=255)
                                                            gtccTTCTTTCTTTTGGGGGGGGGGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTgcgc                           <  2:8334277/99‑1 (MQ=18)
                                                              ccTTCTTTCTTGGGGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTgcgcgc                         <  1:9073136/101‑1 (MQ=17)
                                                                 tctttcttTTCGGGGGGGCGGG.GGGGGAAG...CCCCCCCCGGGG...C...CG.CCCCGGGCTTGCCCCGGGG.GAAACCTT.CCCCCC.CGAAAAACCTGCCCgcgccccgc                      >  1:8914027/1‑96 (MQ=255)
                                                                 tctttcttTTCGGGGGGGCGGG.GGGGGAAG...CCCCCCCCG.GG...G...GG.CCCCGGGGTTGCCCCCGGC.GGAAGCTTCCCCCCCGGGAAAACCTTGccgccccgccc                       >  2:907546/1‑92 (MQ=255)
                                                                  tttccttgTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCg                     <  2:9003824/97‑1 (MQ=18)
                                                                   ttccttgtggggggggGGGC.GGGTGAAG...CCCCCCGCG.GG...G...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGc                    <  1:922659/92‑1 (MQ=18)
                                                                     tttttgggggggggGGGG.GGGTGAGC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCtt                  <  1:2135367/94‑1 (MQ=17)
                                                                     tctttTCGGGGGGGCGGG.GGGGGGAGGC.CCCCCCGG..GG...C...GG.CCCGGGGGTGGCCCGGGGG.GCAAGCTT.CCCCCCCCGAAAACCTGCCCTGCGCGCGGCGCt                   >  1:1162582/1‑101 (MQ=255)
                                                                      ttttgggggggggGGGG.GGGGGAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt                 <  1:383728/95‑1 (MQ=17)
                                                                      ctttTGGGGGGGGGGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt                 <  2:4139532/101‑1 (MQ=18)
                                                                      ctttTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt                 <  1:4139230/101‑1 (MQ=21)
                                                                      ctttTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt                 <  1:5165026/101‑1 (MQ=21)
                                                                      ctttTCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt                 <  2:2084553/101‑1 (MQ=21)
                                                                      ctttTCGGGGGGGCGGG.GGGGGAAGGC.CCCCCCGG..GG...G...GG.CCCCGGGGTGGCCCGGGGG.GAAGGGTC.CCCCCCGCGAAAACCTTTCC.GCGCGCGCCggctt                 >  2:6227605/1‑97 (MQ=255)
                                                                       tttcCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTc                <  2:3750071/101‑1 (MQ=18)
                                                                        tttCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTcc               <  1:56048/101‑1 (MQ=21)
                                                                        tttCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...G...CG.GCTCGGGGTTGCCCGGGGG.GAAAGCGT.TCCCCCGGGAAAAACTTTGCTGCGCGCGTGGGTTTcc               >  1:1338346/1‑101 (MQ=255)
                                                                        tttCGGGGGGGCGGG.GGGCGAAG...CCCCCCGCG.GG...G...CG.CCTCCGGGTTGCCCGAGGC.GGAAGCTT.CCCCCCCCGAAAAACCTGCCTGCGCGCGGCCCCTTcc               >  2:6345974/1‑101 (MQ=2)
                                                                        ttgggggggggGGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTcc               <  1:4961944/97‑1 (MQ=18)
                                                                         ttCGGGGGGGCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTccc              <  2:3511610/101‑1 (MQ=21)
                                                                         ttCGGGGGGGCGGG.GGGGGAAG...CCCCCCCCG.GG...G...CG.CCCCCGGCTTGCCCCCGGCGGAAACATT.CCCCCCCGGAAACACTT.CCTGGGCGCGGCGCTTTcgc              >  2:5968239/1‑99 (MQ=255)
                                                                          tggggggggGGGG.GGGGGAGG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCt             <  1:322251/99‑1 (MQ=18)
                                                                           cGGGGGGGCGTG.GGTGGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCtt            <  2:2246000/101‑1 (MQ=18)
                                                                           cGGGGGGGCGGG.GGGGGAAG...CCCCCCGCG.GG...G...CG.CCCCCGGCGTGCCCGCGGCGGAAACGTC.CCCCCCCCGAAAACC.TGCCCCCGCGCGGCGCTTTCCCtt            >  2:8016072/1‑101 (MQ=255)
                                                                             ggggggCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTc          <  2:8516181/101‑1 (MQ=21)
                                                                             ggggggCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTc          <  2:1136105/101‑1 (MQ=21)
                                                                              gggggCGGG.GGGTGACG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCg         <  2:5301255/101‑1 (MQ=21)
                                                                                gggGGGG.GGGTGAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGc       <  1:6303815/101‑1 (MQ=21)
                                                                                gggCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGc       <  1:5688135/101‑1 (MQ=21)
                                                                                gggCGGG.GGGGGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGc       <  2:8008591/101‑1 (MQ=21)
                                                                                 ggCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGcc      <  1:331132/101‑1 (MQ=21)
                                                                                  gCGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGccc     <  2:3950001/101‑1 (MQ=21)
                                                                                   cGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCg    <  2:1699815/101‑1 (MQ=21)
                                                                                   cGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCg    >  1:3962372/1‑101 (MQ=21)
                                                                                   cGGG.GGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCg    >  2:267526/1‑101 (MQ=21)
                                                                                    ggg.gggTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGccccg   >  2:5569540/1‑99 (MQ=21)
                                                                                    ggg.gggTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCg  >  1:7707072/1‑100 (MQ=21)
                                                                                    ggg.gggTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGCCGCTTTCCCTTTCGGCCCgg   >  2:705857/1‑101 (MQ=21)
                                                                                     gg.gggTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCGgc  <  2:2717010/101‑1 (MQ=21)
                                                                                                         |                                                                                              
CTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGG.GGGTGAAG...CCCCCC....GT...C...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCGGC  >  NC_014034/1732766‑1732948

Base quality scores:  ATCG  < 3 ≤  ATCG  < 10 ≤  ATCG  < 22 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG