Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,841 +C 100% intergenic (‑94/+99) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8411.→C100.0% 26.0 402intergenic (‑94/+99)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (133/256):  ref base (10/2):  total (143/259)
Fisher's exact test strand distribution p-value = 8.51e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.64e-01

Only 100 of 470 total aligned reads displayed.

GTGTCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCC  >  NC_014034/1734741‑1734936
                                                                                                     |                                                                                                 
gtgtCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTg                                                                                                    <  1:779313/101‑1 (MQ=255)
  gtCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.cc                                                                                                 >  1:2173121/1‑101 (MQ=255)
   tCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.acc                                                                                                >  2:3775956/1‑101 (MQ=255)
    cATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGGGCGG.gccc                                                                                               >  1:6890821/1‑101 (MQ=255)
    cATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.cccc                                                                                               <  1:4372931/101‑1 (MQ=255)
    cATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.cccc                                                                                               >  1:820443/1‑101 (MQ=255)
      tCGAACCCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.cccccc                                                                                             <  1:5378509/101‑2 (MQ=255)
      tCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.cccccc                                                                                             <  1:4271808/101‑2 (MQ=255)
       cGAACCCTCCGGGGAATGGCGCATCCGAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.ccccccg                                                                                            <  2:815249/101‑3 (MQ=255)
           cccTCCGGGGAATGGCGCATCCTGGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCt                                                                                         <  2:760666/100‑1 (MQ=255)
           aCCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCt                                                                                         <  2:1140891/101‑1 (MQ=255)
           aCCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCt                                                                                         <  2:7400653/101‑1 (MQ=255)
            ccTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTg                                                                                        <  2:1246971/101‑1 (MQ=255)
               ccGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCgg                                                                                     <  1:2059481/101‑1 (MQ=255)
               ccGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCgg                                                                                     <  1:759856/101‑1 (MQ=255)
                 ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGAc                                                                                   >  2:50774/1‑101 (MQ=255)
                 ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGAc                                                                                   <  1:4465122/101‑1 (MQ=255)
                    gAATGGCGCCTCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTcc                                                                                <  1:855291/101‑1 (MQ=255)
                    gAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTcc                                                                                >  1:2406338/1‑101 (MQ=255)
                      aTGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTcccc                                                                              <  1:6388378/101‑1 (MQ=255)
                       tGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTccccc                                                                             >  1:2889286/1‑101 (MQ=255)
                        ggCGCATCCTAGCGGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTcccccc                                                                            <  2:6903217/101‑1 (MQ=255)
                        ggCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTcccccc                                                                            <  2:5503236/101‑1 (MQ=255)
                        ggCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTcccccc                                                                            <  1:8501859/101‑1 (MQ=255)
                        ggCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTcccccc                                                                            >  1:5661833/1‑101 (MQ=255)
                        gccgcATCCTAGCGGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTcccccc                                                                            <  2:1518770/99‑1 (MQ=255)
                           gcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCggg                                                                         <  1:1010871/101‑1 (MQ=255)
                           gcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCcgg                                                                         >  2:3964431/1‑101 (MQ=255)
                           gcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACACTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCtgg                                                                         <  1:7451114/101‑1 (MQ=37)
                             aTCCTGGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGat                                                                       >  1:6032346/1‑101 (MQ=255)
                             aTCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGat                                                                       <  1:8975668/101‑1 (MQ=255)
                             aTCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGat                                                                       <  2:2855532/101‑1 (MQ=255)
                              tCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGata                                                                      <  2:4188474/101‑1 (MQ=255)
                              tCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACACTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGata                                                                      >  1:4158859/1‑101 (MQ=255)
                              cccTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGata                                                                      <  2:8649704/100‑1 (MQ=255)
                               ccTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGatat                                                                     >  2:8842719/1‑101 (MQ=255)
                                 cagcaggCGGGGCGGGAAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATAttt                                                                   <  1:2304494/100‑1 (MQ=21)
                                   gcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAt                                                                 <  2:1295349/101‑1 (MQ=255)
                                    cagcCGGGGCGGGCAAGCCAAAGGCTGACTCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATg                                                                <  2:2246596/101‑1 (MQ=255)
                                     agcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATgg                                                               <  2:6468882/101‑1 (MQ=255)
                                     agcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATgg                                                               <  1:272854/101‑1 (MQ=255)
                                       ccGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCa                                                             <  2:2741376/101‑1 (MQ=255)
                                         ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAg                                                           >  2:5933799/1‑101 (MQ=255)
                                         ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGGCCTGCGGACTCCCCCCGGGCCATTTATGGCAAg                                                           >  1:1814501/1‑101 (MQ=21)
                                          gggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGa                                                          <  1:4683634/101‑1 (MQ=255)
                                             cgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGa                                                       <  2:935459/101‑1 (MQ=255)
                                              gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGaa                                                      <  1:9165970/101‑1 (MQ=255)
                                              gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGaa                                                      >  2:4542019/1‑101 (MQ=255)
                                               ggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGaaa                                                     >  1:143622/1‑101 (MQ=255)
                                               ggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGaaa                                                     <  1:3865561/101‑1 (MQ=255)
                                                  aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTg                                                  <  1:6522569/101‑1 (MQ=255)
                                                     ccAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.gg                                              <  2:156579/101‑1 (MQ=255)
                                                      cAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGc                                             >  2:5204051/1‑101 (MQ=255)
                                                       aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGcc                                            >  2:6170965/1‑101 (MQ=255)
                                                         aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCtt                                          <  2:2026098/101‑1 (MQ=255)
                                                          ggCTGCCGCTGCGTCAGGCGACGGGCCGGGAAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                         <  1:3762682/101‑1 (MQ=18)
                                                          ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                         <  1:4603959/101‑1 (MQ=255)
                                                          ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                         >  2:1680844/1‑101 (MQ=255)
                                                          ggCTGACGCTGCGTCAGCCGACGGGCCGGGCACGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                         <  1:6111013/101‑1 (MQ=255)
                                                          ggCTGACGCGGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                         <  2:1440327/101‑1 (MQ=255)
                                                             tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATc                                      <  2:3042651/101‑1 (MQ=255)
                                                             tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATc                                      >  1:3708572/1‑101 (MQ=255)
                                                             tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGCATATTTATGGCCAGATGAAACTGA.GGCCTTCATc                                      >  1:2556971/1‑101 (MQ=18)
                                                              gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                     <  1:882539/101‑1 (MQ=255)
                                                               gcgctcgggcAGCCGAGGGGCCGGGCAGGGGTGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCGTTCATCtt                                    <  2:319987/92‑1 (MQ=14)
                                                                 gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                  >  1:2993725/1‑101 (MQ=255)
                                                                 gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                  <  2:5614227/101‑1 (MQ=255)
                                                                     ggTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                              <  2:611180/100‑1 (MQ=255)
                                                                     cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                              <  1:1624735/101‑1 (MQ=255)
                                                                       tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                            <  2:470716/101‑1 (MQ=255)
                                                                            cGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                       <  1:5082543/101‑1 (MQ=255)
                                                                            cGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                       <  1:3371434/101‑1 (MQ=255)
                                                                             gACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                      <  1:5348978/101‑1 (MQ=255)
                                                                             gACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                      <  2:3692941/101‑1 (MQ=255)
                                                                              aCGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                    <  2:3264977/101‑1 (MQ=255)
                                                                               cgggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gg                   <  2:65692/101‑1 (MQ=255)
                                                                                gggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.ggg                  >  2:5295177/1‑101 (MQ=255)
                                                                                gggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.ggg                  >  2:8551026/1‑101 (MQ=255)
                                                                                 ggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gggg                 <  1:4905410/101‑1 (MQ=255)
                                                                                  tgacggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg               >  2:2469975/9‑101 (MQ=2)
                                                                                  tgacggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg               >  1:3206631/9‑101 (MQ=2)
                                                                                  tgacggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg               >  1:3238446/9‑101 (MQ=2)
                                                                                    cgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc              <  2:6360949/101‑1 (MQ=255)
                                                                                    acggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa             >  1:1319776/7‑99 (MQ=2)
                                                                                     gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg             <  1:6971503/101‑1 (MQ=255)
                                                                                     gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg             >  2:7116777/1‑101 (MQ=255)
                                                                                      ggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgg            >  1:6717405/1‑101 (MQ=255)
                                                                                      ggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgg            >  2:2625040/1‑101 (MQ=255)
                                                                                         tggggggCGCTGCCCCCCGTGCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc         <  1:7254596/100‑1 (MQ=255)
                                                                                         aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc         >  2:587406/1‑101 (MQ=255)
                                                                                           gggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCag       >  2:2955981/1‑101 (MQ=255)
                                                                                           gggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCag       <  1:7110037/101‑1 (MQ=255)
                                                                                            ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCaga      >  2:7742896/1‑101 (MQ=255)
                                                                                            ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCaga      <  2:6227666/101‑1 (MQ=255)
                                                                                             gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGAGTATGTCTGGCACGATGAAACTGC.GCCCTTCATATTGCCATAAATATCCC.GGGATGCGAGGCagag     >  2:2310273/1‑101 (MQ=14)
                                                                                              ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGc    <  2:4278625/101‑1 (MQ=255)
                                                                                              ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGc    >  2:1053600/1‑101 (MQ=255)
                                                                                               gcgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcc   <  2:8489388/101‑1 (MQ=255)
                                                                                               gcgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcc   <  2:3465391/101‑1 (MQ=255)
                                                                                                cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGccc  <  2:671030/101‑1 (MQ=255)
                                                                                                     |                                                                                                 
GTGTCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCC  >  NC_014034/1734741‑1734936

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG