Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,745,454 +C 1.5% intergenic (‑114/+654) RCAP_rcc01613 ← / ← metH4 hypothetical protein/methionine synthase subunit B

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,745,4542.→C 1.5% 3.8 ‑0.5 ‑0.0269intergenic (‑114/+654)RCAP_rcc01613/metH4hypothetical protein/methionine synthase subunit B
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (142/123):  total (145/124)
Fisher's exact test strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.04e-01

Only 100 of 356 total aligned reads displayed.

GCAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CG.G.CGCGAGCTTTGGCACCT.C.A.CCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGC.GGGCCCGAAGCGCAAGGCGCCCGG  >  NC_014034/1745354‑1745553
                                                                                                          |                                                                                                     
 cAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCCCCT.C.A.c                                                                                                      >  1:4156983/1‑101 (MQ=255)
   gcgcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.A.ccc                                                                                                    <  1:2959584/101‑1 (MQ=255)
      ccccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.A.cccccc                                                                                                 <  2:2403583/101‑1 (MQ=255)
      ccccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.A.cccccc                                                                                                 <  1:2766218/101‑1 (MQ=255)
       cccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.A.cccccc.c                                                                                               >  1:4853440/1‑101 (MQ=255)
          ggTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.A.cccccc.cccg                                                                                            >  2:846216/1‑99 (MQ=255)
          ggTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCAAGCGGCATGTCGATCGCGGCGGGCGA.CGCG.CGCGAGC.TTGGCACCT.C.A.cccccc.cccc                                                                                            >  2:4448404/1‑99 (MQ=255)
           gTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.C.cccccc.cccgg                                                                                           >  2:1583033/1‑98 (MQ=255)
            tGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGGCGACCC.G.GGCGAGC.TTGGCACCT.C.C.cccccc.ccccgc                                                                                          >  1:7343473/1‑97 (MQ=255)
            ggcggggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGAGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.A.cccccc.cccggc                                                                                          <  1:954427/100‑5 (MQ=255)
             gcggggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.A.cccccc.ccccgcg                                                                                         >  1:6131811/1‑96 (MQ=255)
              cggggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.A.cccccc.ccccgcgc                                                                                        >  2:4123463/1‑95 (MQ=255)
                gggggcgggggTCGGCAGCCCGGCCGTCGGATCGGGCAGCGGCATGTCGATCGCGGCGGTCGG.CGCG.CGGGAGC.TTGGCACCT.C.A.cccccc.cccggggcgc                                                                                      >  2:8969063/1‑93 (MQ=255)
                gggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGGGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCAc                                                                                       >  2:6588736/1‑101 (MQ=255)
                gggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCAc                                                                                       <  2:1879616/101‑1 (MQ=255)
                gggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCAc                                                                                       <  2:580077/101‑1 (MQ=255)
                gggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.A.cccccc.ccccgcgccc                                                                                      >  1:8043755/1‑93 (MQ=255)
                 ggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCAcc                                                                                      <  1:8362969/101‑1 (MQ=255)
                 ggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCAcc                                                                                      <  2:7014435/101‑1 (MQ=255)
                     cgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCCCCGGcc                                                                                  >  2:3917051/1‑100 (MQ=255)
                      gggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGcac                                                                                 <  1:6560963/101‑1 (MQ=255)
                       ggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGcaca                                                                                <  2:8058417/101‑1 (MQ=255)
                          gTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCCGCGCACCGGCACAGcc                                                                             >  2:6213194/1‑101 (MQ=255)
                          gTCGGCAACCCGGCCGGCGGAATGGCCAACGGCTTGTCTGACGCGGCGGTAGA.CGCG.CGCGA.CCTTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGcc                                                                             <  2:1094081/101‑1 (MQ=255)
                             ggtggCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCaga                                                                          <  2:7496613/97‑1 (MQ=255)
                             ggCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCaga                                                                          <  1:7076903/101‑1 (MQ=255)
                             ggCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGGCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCCGACCGGCACAGCCaca                                                                          >  2:2818270/1‑99 (MQ=255)
                              gCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCagag                                                                         <  1:9037837/101‑1 (MQ=255)
                              gCAGCCCGGCCGTCGGACCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCTCC.C.CCCCCC.CCGGCGCACCGGCACAGCCagag                                                                         <  1:4520773/101‑1 (MQ=255)
                               cAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCagaga                                                                        <  2:4169063/101‑1 (MQ=255)
                                aGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCagagag                                                                       <  1:5391822/101‑1 (MQ=255)
                                aGCCCGGCCGTCGGATCGGCCAGCGGCATCTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCTCC.C.CCCCCC.CCGGCGCACCGGCACAGCCagagag                                                                       <  1:5805856/101‑1 (MQ=255)
                                 gCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAgc                                                                      <  2:680527/101‑1 (MQ=255)
                                 gCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAgc                                                                      <  1:3725484/101‑1 (MQ=255)
                                     ggCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCTGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCgcgg                                                                  <  1:8179903/101‑1 (MQ=255)
                                      gCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCgcggc                                                                 <  2:672437/101‑1 (MQ=255)
                                       ccGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCgcggcg                                                                >  1:3511420/1‑101 (MQ=255)
                                        cGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGcggcgg                                                               >  1:4313530/1‑101 (MQ=255)
                                        cGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCCGCGCCCCGGCACAGCCAGAGAGCGcggcgg                                                               >  2:6343404/1‑101 (MQ=255)
                                         gTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCggcggc                                                              >  2:5104997/1‑101 (MQ=255)
                                           cGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCa                                                            <  1:3566429/101‑1 (MQ=255)
                                            ggATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCATAGAGCGCGGCGGcccg                                                           >  1:9050072/1‑99 (MQ=255)
                                               tCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagc                                                        >  2:1218487/1‑101 (MQ=255)
                                                cGGCCGGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcc                                                       <  2:8251504/101‑1 (MQ=255)
                                                cGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcc                                                       <  2:7303559/101‑1 (MQ=255)
                                                cGGCCAGCGGCAGGTAGATCGCGGCCGTCGA.CGCG.CGCGAGC.TTGGCACCT.CGC.CCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcc                                                       <  2:9015669/101‑1 (MQ=255)
                                                 ggCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcca                                                      <  1:8735950/101‑1 (MQ=255)
                                                 ggCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcca                                                      <  1:3289863/101‑1 (MQ=255)
                                                 ggCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcca                                                      <  1:3139471/101‑1 (MQ=255)
                                                  gCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcccg                                                     >  1:1180618/1‑99 (MQ=255)
                                                  gCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagccag                                                     <  2:1673604/101‑1 (MQ=255)
                                                    cAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagccagcc                                                   >  2:3227608/1‑101 (MQ=255)
                                                    cAGCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagccagcc                                                   >  1:8270002/1‑101 (MQ=255)
                                                      gCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCTCC.C.CCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGcccc                                                 <  2:7755726/101‑1 (MQ=255)
                                                      gCGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGcccc                                                 <  2:7375962/101‑1 (MQ=255)
                                                      gCGGCATGTCGATCGCGGCGGTCGA.CGCG.CACGAGC.TTGGCACCTCC.C.CCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGcccc                                                 <  2:3461789/101‑1 (MQ=255)
                                                       cGGCATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCg                                                <  1:67521/101‑1 (MQ=255)
                                                          cATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCgcgg                                             >  1:6063523/1‑98 (MQ=255)
                                                          cATGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCgaga                                             >  1:8445363/1‑101 (MQ=255)
                                                           aggTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGagag                                            <  2:4741343/99‑1 (MQ=255)
                                                           aTGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGagag                                            >  1:6931766/1‑101 (MQ=255)
                                                           aTGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGagag                                            <  2:8553346/101‑1 (MQ=255)
                                                           aTGTCGATCGCGGCGGTCGA.CGCG.CCCGGGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGagag                                            <  2:3342961/101‑1 (MQ=255)
                                                            tGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAgg                                           >  1:5375704/1‑101 (MQ=255)
                                                            tGTCGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.A.CCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAggg                                          >  1:2840729/1‑101 (MQ=255)
                                                              gcGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCCCCTCC.C.CCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGc                                         <  2:8779521/100‑1 (MQ=255)
                                                              gcGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCTCC.C.CCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGc                                         <  2:3090023/100‑1 (MQ=255)
                                                               cGATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCa                                        <  1:5516699/101‑1 (MQ=255)
                                                                gATCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAg                                       >  1:7510024/1‑101 (MQ=255)
                                                                 aTCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCTCC.C.CCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGt                                      <  1:7687274/101‑1 (MQ=255)
                                                                  tCGCGGCGGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTc                                     <  1:1848184/101‑1 (MQ=255)
                                                                    gcggcgGTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCaa                                   >  1:3359189/1‑101 (MQ=255)
                                                                     cggcggTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAg                                  <  1:1099070/101‑1 (MQ=255)
                                                                       gcggTCGA.CGCG.CGCGAGC.TTGGCACCTCC.C.CCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGa                                <  1:1588885/101‑1 (MQ=255)
                                                                       gcggGCGA.CG.CCCGCGAGC.TTGGCCCCT.C.C.CCCCCCGCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGa                                <  1:8218668/101‑1 (MQ=255)
                                                                         ggTCGA.CGCG.CGCGAGC.TTGGCACCTCC.C.CCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAc                              <  1:8934668/101‑1 (MQ=255)
                                                                         ggTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAc                              <  2:4853280/101‑1 (MQ=255)
                                                                         ggTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAc                              >  1:2308190/1‑101 (MQ=255)
                                                                         ggTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAc                              <  1:7835771/101‑1 (MQ=255)
                                                                         ggTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCCGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCAAGAGGGCAGTCAAGGAAc                              >  1:439969/1‑101 (MQ=255)
                                                                          gTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcg                             >  1:2123977/1‑101 (MQ=255)
                                                                          gTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcg                             >  2:5360183/1‑101 (MQ=255)
                                                                          gTCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcg                             >  1:4696114/1‑101 (MQ=255)
                                                                           tCGA.CGCG.CGCGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcgg                            <  1:2626435/101‑1 (MQ=255)
                                                                             cgacgcg.cgcGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcggc                           >  2:842133/7‑101 (MQ=255)
                                                                              gacgcg.cgcGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGACAGGGCAGTCAAGGAAcggc.g                         >  1:5649120/6‑101 (MQ=255)
                                                                                 gcg.cgcGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGC.GGGc                      <  2:7825345/99‑1 (MQ=255)
                                                                                   g.cgcGAGC.TTGGCACCTCC.C.CCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGC.GGGccc                    <  2:2308123/101‑1 (MQ=255)
                                                                                   g.cgcGAGC.TTGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGC.GGGccc                    <  2:5671628/101‑1 (MQ=255)
                                                                                         gagcttGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGC.GGGccccacg                >  1:6372962/5‑97 (MQ=255)
                                                                                           gcttGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGC.GGGGCCCCAgcg              >  1:1430881/3‑101 (MQ=255)
                                                                                            cttGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGCTGCAGCCCCGAGAGGGCAGTCAAAGAACGGG.GGGACCTAGgcgc             >  2:4555334/2‑101 (MQ=255)
                                                                                            cttGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGGACGGC.GGGgcccagacgc             >  2:2574684/2‑94 (MQ=255)
                                                                                            cttGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGC.GGGCCCGAAacgc             >  1:3856747/2‑101 (MQ=255)
                                                                                             ttGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCCGaccgca             >  1:1749010/1‑96 (MQ=255)
                                                                                             ttGGCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGC.GGGCCCGAAGCGCa            <  2:7645000/101‑1 (MQ=255)
                                                                                             ttGGCACCC.C.CCCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGC.GGGCCCGAAGCGCa            <  2:8880007/101‑1 (MQ=255)
                                                                                               ggCACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGC.GGGCCCGAAGCGCAAg          <  1:2432556/101‑1 (MQ=255)
                                                                                                 cACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGC.GGGGCCGCAgcgccaaag        >  1:5085622/1‑96 (MQ=255)
                                                                                                 cACCT.C.ACCCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGC.GGGCCCGAAGCGCAAGgc        <  2:5493930/101‑1 (MQ=255)
                                                                                                          |                                                                                                     
GCAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CG.G.CGCGAGCTTTGGCACCT.C.A.CCCCCC.CCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGC.GGGCCCGAAGCGCAAGGCGCCCGG  >  NC_014034/1745354‑1745553

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG