Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,763,083 +C 98.8% coding (338/1338 nt) RCAP_rcc01630 → M50 family peptidase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,763,0831.→C98.8% 2.6 ‑0.5 ‑0.9335coding (338/1338 nt)RCAP_rcc01630M50 family peptidase
Reads supporting (aligned to +/- strand):  new base (144/187):  ref base (1/3):  total (145/190)
Fisher's exact test strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.15e-01

Only 100 of 347 total aligned reads displayed.

CGATGCCAACGCCACTTCGGCGGGGGTGGACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.G.G.GGGCGGCGACCGTGGCCGC.G.GGG.C.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAGGGCAAGCCCGCCGTG  >  NC_014034/1762985‑1763177
                                                                                                      |                                                                                                  
cGATGCCAACGCCACTTCGGCGGGGGTGGACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTgg.g.c.gg                                                                                                  >  2:8928109/1‑101 (MQ=255)
          gCCACTTCGGCGGGGGTGGACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGAc                                                                                          >  2:2187144/1‑101 (MQ=255)
          gCCACTTCGGCGGGGGTGGACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGAc                                                                                          >  2:2548176/1‑101 (MQ=255)
            cACTTCGGCGGGGGTGGACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCg                                                                                        >  1:7029368/1‑101 (MQ=255)
              cTTCGGCGGGGGGGGACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGtt                                                                                      <  2:2757188/101‑2 (MQ=255)
              cTTCGGCGAGGGTGGACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGtt                                                                                      <  2:8614775/101‑2 (MQ=255)
                tCGGCGGGGGTGGACGAGGAAACCATGGCGCGGCTGAACGCCGAGGGACGCCGCCATTCGATGCCTGGCGCGCCCC.TTTGG.GCGCGGGGGGCGACCGTTGc                                                                                    >  1:8951706/1‑101 (MQ=255)
                      ggggTGGACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.g.gg                                                                            >  1:7042778/1‑101 (MQ=255)
                         gTGGACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGG.cc                                                                        <  2:1109238/101‑2 (MQ=255)
                         gTGGACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGG.cc                                                                        <  1:2627193/101‑2 (MQ=255)
                         gTGGACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGG.cc                                                                        <  2:6436359/101‑2 (MQ=255)
                          tGGACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGG.ccg                                                                       <  1:2500274/101‑3 (MQ=255)
                          tGGACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGG.ccg                                                                       >  1:8366370/1‑99 (MQ=255)
                           ggACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGG.ccgg                                                                      >  1:8892291/1‑98 (MQ=255)
                           ggACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGG.ccgg                                                                      >  1:5275474/1‑98 (MQ=255)
                           ggACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGG.ccgg                                                                      >  1:392824/1‑98 (MQ=255)
                            gaggAGGGACCCATGGCGCGTCTGAACGCCGAGGAACGCCGCCATTCGATGCAGGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGG.ccggt                                                                     <  2:6843491/98‑5 (MQ=255)
                              cGAGGAAACCATGGCGCGGCTGAACGCCGAGGACCGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCt                                                                   <  2:366870/101‑1 (MQ=255)
                              cGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCt                                                                   <  1:6805377/101‑1 (MQ=255)
                              cGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCt                                                                   <  1:946484/101‑1 (MQ=255)
                               gAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCtt                                                                  >  2:1373596/1‑101 (MQ=255)
                               gAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCtt                                                                  >  2:1537584/1‑101 (MQ=255)
                               gAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCtt                                                                  <  1:3339620/101‑1 (MQ=255)
                                aGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTc                                                                 <  1:2787857/101‑1 (MQ=255)
                                 ggAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCa                                                                <  1:8024037/101‑1 (MQ=255)
                                  gAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCaa                                                               <  2:3089688/101‑1 (MQ=255)
                                    atccATGGCGCGCCTGAACGCTGAGGAACGACGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACt                                                             <  1:9092972/99‑1 (MQ=255)
                                      ccATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTc                                                           <  2:4459292/101‑1 (MQ=255)
                                      cagtGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTc                                                           <  2:124927/98‑1 (MQ=255)
                                       cATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCa                                                          <  1:6924689/101‑1 (MQ=255)
                                       cATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCa                                                          <  2:6250927/101‑1 (MQ=255)
                                        ctcgcgcGCCTGAACGCCGAGGAACTCCGCCATTCGATGCATGGCCCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.CGTCTTCAACTTCAt                                                         <  2:4233087/98‑1 (MQ=255)
                                        aTGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGGCTTCAACTTCAt                                                         >  1:3385139/1‑101 (MQ=255)
                                         tGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATc                                                        >  1:1846278/1‑101 (MQ=255)
                                            cgcgGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTg                                                     <  1:8192327/101‑1 (MQ=255)
                                             gcgGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGt                                                    <  1:1099005/101‑1 (MQ=255)
                                              cgGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTc                                                   >  1:1560431/1‑101 (MQ=255)
                                                gCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGa                                                 >  1:6190474/1‑101 (MQ=255)
                                                gCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGa                                                 >  1:7917503/1‑101 (MQ=255)
                                                 cTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGAt                                                >  2:4564105/1‑101 (MQ=255)
                                                  tGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATc                                               <  1:5928404/101‑1 (MQ=255)
                                                  tGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATc                                               >  1:4825755/1‑101 (MQ=255)
                                                   gAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATcc                                              <  2:4342100/101‑1 (MQ=255)
                                                   gAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATcc                                              >  1:4164690/1‑101 (MQ=255)
                                                   gAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATcc                                              >  2:4679150/1‑101 (MQ=255)
                                                      cGCCGAGGACCGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTgg                                           <  2:3528791/101‑1 (MQ=255)
                                                       gCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGt                                          >  1:3125137/1‑101 (MQ=255)
                                                         cGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCt                                        >  2:6674241/1‑101 (MQ=255)
                                                          gAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCtt                                       <  2:7818549/101‑1 (MQ=255)
                                                          gAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGGCTTCAACTTCATCCTGTCGCTCCTGGTCtt                                       >  2:5401136/1‑101 (MQ=255)
                                                            ggAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTg                                     <  2:1655851/101‑1 (MQ=255)
                                                            ggAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTg                                     <  1:4482349/101‑1 (MQ=255)
                                                            ggAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTg                                     <  2:5359838/101‑1 (MQ=255)
                                                            ggAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTg                                     <  2:2990004/101‑1 (MQ=255)
                                                             gAACGCCGCCATTCGATGCATGGCGCGTCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGc                                    >  2:8906626/1‑101 (MQ=255)
                                                             gAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGc                                    <  2:4135217/101‑1 (MQ=255)
                                                              aaCGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGcc                                   >  2:3810914/1‑101 (MQ=255)
                                                              aaCGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGcc                                   <  1:7609385/101‑1 (MQ=255)
                                                              aaCGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGGCTTTgac                                   >  2:1856399/1‑99 (MQ=255)
                                                               aCGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCg                                  >  2:555061/1‑101 (MQ=255)
                                                                cgccgcCATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCgg                                 <  1:8521836/101‑1 (MQ=255)
                                                                 gccgccATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.GTGGC.C.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCggg                                <  2:4793697/101‑1 (MQ=255)
                                                                  ccgccATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGAGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCgggg                               >  2:5341297/1‑101 (MQ=255)
                                                                   cgccATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGc                              <  1:5534723/101‑1 (MQ=255)
                                                                   cgccATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGc                              <  2:2495890/101‑1 (MQ=255)
                                                                    gccATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCg                             <  2:3347019/101‑1 (MQ=255)
                                                                      cATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGAt                           >  1:2434339/1‑101 (MQ=255)
                                                                      cATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGAt                           >  2:1767432/1‑101 (MQ=255)
                                                                      cATTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTCCATCCTGTCGATCCTGGTCTTTGCCGGGGCGAt                           <  1:6460599/101‑1 (MQ=255)
                                                                       aTTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGTTg                          >  1:5821175/1‑101 (MQ=255)
                                                                       aTTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATg                          <  2:2876729/101‑1 (MQ=255)
                                                                       aTTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATg                          <  2:5098943/101‑1 (MQ=255)
                                                                       aTTCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTGGCCGGGGCGATg                          <  1:6546676/101‑1 (MQ=255)
                                                                        ttCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATgg                         >  1:4042114/1‑101 (MQ=255)
                                                                         tCGATGCATGGCGCGCCCCTTTTGGCG.C.GGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGc                        <  2:5290810/101‑1 (MQ=255)
                                                                         tCGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGc                        <  1:532261/101‑1 (MQ=255)
                                                                         tCGATGCATGGCGCGCCCC.TATGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGACGGc                        <  1:7202832/101‑1 (MQ=255)
                                                                          cGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGcc                       >  2:3654681/1‑101 (MQ=255)
                                                                          cGATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGcc                       >  2:8231301/1‑101 (MQ=255)
                                                                           gATGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCt                      >  2:653752/1‑101 (MQ=255)
                                                                            aTGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTg                     >  1:1285644/1‑101 (MQ=255)
                                                                             tGCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTgg                    >  1:5029092/1‑101 (MQ=255)
                                                                              gCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTggg                   >  1:7270866/1‑101 (MQ=255)
                                                                              gCATGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTggg                   >  2:8102294/1‑101 (MQ=255)
                                                                                aTGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAg                 >  1:6959176/1‑101 (MQ=255)
                                                                                aTGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAg                 <  1:2976618/101‑1 (MQ=255)
                                                                                aTGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAg                 <  2:4041999/101‑1 (MQ=255)
                                                                                aTGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGCCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAg                 >  1:7507967/1‑101 (MQ=255)
                                                                                 tGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAgg                >  2:8517637/1‑101 (MQ=255)
                                                                                 tGGCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAgg                <  1:3705095/101‑1 (MQ=255)
                                                                                  ggCGCGCCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAggg               >  2:4747236/1‑101 (MQ=255)
                                                                                   gcgcgcCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGG.CTGGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAGGGc              <  2:7252442/101‑1 (MQ=255)
                                                                                    cgcgcCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAGGGCa             <  2:7613767/101‑1 (MQ=255)
                                                                                     gcgcCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAGGGCaa            >  2:5931171/1‑101 (MQ=255)
                                                                                     gcgcCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAGGGCaa            <  2:6987505/101‑1 (MQ=255)
                                                                                     gcgcCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAGGGCaa            >  1:8725136/1‑101 (MQ=255)
                                                                                     gcgcCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAGGGCaa            <  2:2106591/101‑1 (MQ=255)
                                                                                      cgcCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGCGG.GGG.C.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAGGGCAAg           >  1:1429057/1‑101 (MQ=255)
                                                                                      cgcCCC.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAGGGCAAg           >  1:1053203/1‑101 (MQ=255)
                                                                                        cccc.TTTGG.GCGCGGGCGGCGACCGTTGCCGC.G.GGGCC.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAGGGCAAGcc         >  1:206439/1‑101 (MQ=255)
                                                                                                      |                                                                                                  
CGATGCCAACGCCACTTCGGCGGGGGTGGACGAGGAAACCATGGCGCGGCTGAACGCCGAGGAACGCCGCCATTCGATGCATGGCGCGCCCC.TTTGG.G.G.GGGCGGCGACCGTGGCCGC.G.GGG.C.GGTCTTCAACTTCATCCTGTCGATCCTGGTCTTTGCCGGGGCGATGGCCTGGGAGGGCAAGCCCGCCGTG  >  NC_014034/1762985‑1763177

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG