BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,769,354	+G	98.4%	coding (426/744 nt)	RCAP_rcc01636 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,769,354	1	.→G	98.4%	5.5 ‑1.0 ‑1.1	304	coding (426/744 nt)	RCAP_rcc01636	hypothetical protein
Reads supporting (aligned to +/- strand): new base (153/145): ref base (4/1): total (157/147)
Fisher's exact test strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.06e-02

Only 100 of 333 total aligned reads displayed.


TGCCGGGCGACGGGCTTTTGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TC.GGCA..GGGGCGGTGGTC.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGG  >  NC_014034/1769254‑1769446


tGCCGGGCGACGGGCTTTTGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.Tc                                                                                                    >  1:2132558/1‑101 (MQ=255)


tGCCGGGCGACGGGCTTTTGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.Tc                                                                                                    >  2:6097824/1‑101 (MQ=255)


ggCCGGGCGACGGGCGTGTGTGCGGCAGCGTGGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.Tc                                                                                                    <  1:2945652/100‑1 (MQ=255)


  ccGGGCGACGGGCTTTTGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TC.gg                                                                                                 >  2:6196795/1‑101 (MQ=255)


  ccGGGCGACGGGCTTTTGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TC.gg                                                                                                 <  1:5553917/101‑1 (MQ=255)


        gACGGGCTTTTGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCA..ggg                                                                                          >  1:1580997/1‑101 (MQ=255)


         aCGGGCTTTTGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCA..gggg                                                                                         >  2:2878278/1‑101 (MQ=255)


          cGGGCTTTTGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCA..ggggg                                                                                        >  1:3279179/1‑100 (MQ=255)


           gggCTTTTGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCA..gggggc                                                                                       <  1:7470954/101‑3 (MQ=255)


            ggCTTTTGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCA..gggggcg                                                                                      >  2:5840316/1‑98 (MQ=255)


             gCTTTTGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCA..gggggcgg                                                                                     >  2:7631232/1‑97 (MQ=255)


               ttttGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCggtg                                                                                    >  1:8445702/1‑101 (MQ=255)


               ttttGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCA..GGGGGggggg                                                                                   >  1:4252252/1‑101 (MQ=255)


               ttttGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGAGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCggtg                                                                                    <  2:1572518/101‑1 (MQ=255)


                tttGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCggtgg                                                                                   >  2:2242845/1‑101 (MQ=255)


                tttGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCggtgg                                                                                   >  1:4591566/1‑101 (MQ=255)


                 ttGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCggtggt                                                                                  <  2:1871595/101‑1 (MQ=255)


                 ttGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCggtggt                                                                                  <  1:863707/101‑1 (MQ=255)


                  tgtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTc                                                                                 >  2:7160264/1‑101 (MQ=255)


                  tgtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTc                                                                                 <  2:8548207/101‑1 (MQ=255)


                  tgtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTc                                                                                 <  1:5848697/101‑1 (MQ=255)


                   gtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTcg                                                                                <  1:7767828/101‑2 (MQ=255)


                   gtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTcg                                                                                >  2:2030122/1‑100 (MQ=255)


                       ggCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTcgcgcg                                                                            >  2:3483747/1‑96 (MQ=255)


                         cAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTcgcgcgcg                                                                          >  2:6957289/1‑94 (MQ=255)


                          aGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGTTG.TCGGGCAG.GGGGCGGTGGTcgcgcgcgg                                                                         >  1:3213819/1‑93 (MQ=255)


                          aGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGGGGTcgcgcgcgg                                                                         >  2:5457960/1‑93 (MQ=255)


                            cGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTcgcgcgcgggc                                                                       >  2:4634842/1‑91 (MQ=255)


                            cGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTcgcgcgcgggc                                                                       <  1:6606478/101‑11 (MQ=255)


                               cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCttt                                                                  <  1:7331075/101‑1 (MQ=255)


                                ggCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTg                                                                 <  1:4006637/101‑1 (MQ=255)


                                   gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCt                                                              >  1:4662597/1‑101 (MQ=255)


                                   gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCt                                                              <  1:4489680/101‑1 (MQ=255)


                                   gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCt                                                              >  1:346395/1‑101 (MQ=255)


                                   gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCt                                                              >  2:2564982/1‑101 (MQ=255)


                                   gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTC.GCGCG.CGGGCTTTGCCt                                                              <  1:4876302/101‑1 (MQ=255)


                                    aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCtt                                                             <  1:6879803/101‑1 (MQ=255)


                                    aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTC.GC.GCGCGGGCTTTGCCtt                                                             <  1:1822318/101‑1 (MQ=255)


                                      cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTgg                                                           >  1:5102384/1‑101 (MQ=255)


                                          aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTc                                                       <  1:5046039/101‑1 (MQ=255)


                                           ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCg                                                      <  1:156963/101‑1 (MQ=255)


                                           ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCg                                                      <  1:2030183/101‑1 (MQ=255)


                                            cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGCCgg                                                     >  2:4999424/1‑100 (MQ=255)


                                             aaCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGGGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAg                                                    >  1:1183347/1‑101 (MQ=255)


                                               cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgc                                                  <  2:5695718/101‑1 (MQ=255)


                                                tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcg                                                 >  1:8994275/1‑101 (MQ=255)


                                                tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcg                                                 >  2:6742596/1‑101 (MQ=255)


                                                tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcg                                                 >  1:3906969/1‑101 (MQ=255)


                                                tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGGGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGGgggg                                                 >  2:4276395/1‑99 (MQ=255)


                                                 ggATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcgc                                                >  1:421168/1‑101 (MQ=255)


                                                 ggATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcgc                                                <  1:7265400/101‑1 (MQ=255)


                                                    tGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTc                                             <  1:2565055/101‑1 (MQ=255)


                                                     gCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTcc                                            >  2:300109/1‑101 (MQ=255)


                                                     gCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTcc                                            <  2:2142867/101‑1 (MQ=255)


                                                         cccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGTTCGATG.TCGGGCAG.GGGGCGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGc                                        <  1:4345242/101‑1 (MQ=255)


                                                          ccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCa                                       >  1:5366041/1‑101 (MQ=255)


                                                           cgcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAc                                      >  2:7758457/1‑101 (MQ=255)


                                                            gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                     <  1:8906259/101‑1 (MQ=255)


                                                            gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                     <  2:4126104/101‑1 (MQ=255)


                                                            gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                     <  1:1348844/101‑1 (MQ=255)


                                                            gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                     <  2:7930444/101‑1 (MQ=255)


                                                            gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                     <  1:7875725/101‑1 (MQ=255)


                                                               cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                  <  2:4031426/101‑1 (MQ=255)


                                                               cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                  <  1:8525197/101‑1 (MQ=255)


                                                               cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                  >  2:8342229/1‑101 (MQ=255)


                                                               cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                  >  2:666154/1‑101 (MQ=255)


                                                                 gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgc                                <  1:7160568/101‑1 (MQ=255)


                                                                   ggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcgc                              >  1:1525910/1‑101 (MQ=255)


                                                                    gtaaTCGTGGGGGTTGTGGGGGGGGTTGATG.GCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCGTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                             <  1:5495520/99‑1 (MQ=255)


                                                                    gAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TC.GGGAAGGGGGCGGGGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCGGCGGTGCGCt                             >  1:8336954/1‑101 (MQ=255)


                                                                     aaaTCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTc                            >  2:6908933/1‑101 (MQ=255)


                                                                     aaaTCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTc                            >  1:7591227/1‑101 (MQ=255)


                                                                      aaTCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTcc                           >  1:5756270/1‑101 (MQ=255)


                                                                         cgtcgCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                        <  2:6499862/101‑1 (MQ=255)


                                                                         cgtcgCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                        >  1:7973524/1‑101 (MQ=255)


                                                                         cgtcgCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                        <  1:1809664/101‑1 (MQ=255)


                                                                         cgtcgCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                        <  1:3648572/101‑1 (MQ=255)


                                                                         cgtcgCGGCTTTGGGCGCGGTCGATG.TCAGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                        <  1:6391100/101‑1 (MQ=255)


                                                                          gtcgCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGa                       >  1:8161286/1‑101 (MQ=255)


                                                                          gtcgCGGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGa                       >  2:2685663/1‑101 (MQ=255)


                                                                            cgcgGCTTTGGGCGCGGTCGATGTTG.GGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                     >  1:2393379/1‑101 (MQ=255)


                                                                            cgcgGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                     >  1:3805646/1‑101 (MQ=255)


                                                                            cgcgGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                     >  2:5284889/1‑101 (MQ=255)


                                                                            cgcgGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                     >  1:3358043/1‑101 (MQ=255)


                                                                            cgcgGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                     <  2:803643/101‑1 (MQ=255)


                                                                            cgcgGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                     <  2:36992/101‑1 (MQ=255)


                                                                             gcgGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                    <  2:9016756/101‑1 (MQ=255)


                                                                             gcgGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                    <  2:5177885/101‑1 (MQ=255)


                                                                              cgGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                   >  2:3348841/1‑101 (MQ=255)


                                                                              cgGCTTTGGGCGCGGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                   >  1:6001690/1‑101 (MQ=255)


                                                                                         gcgGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCa        >  2:3265473/1‑101 (MQ=255)


                                                                                          cgGTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt       <  1:1265162/101‑1 (MQ=255)


                                                                                           ggTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGGATc      >  2:1189845/1‑101 (MQ=255)


                                                                                           ggTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc      >  1:3533710/1‑101 (MQ=255)


                                                                                           ggTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc      >  1:2872143/1‑101 (MQ=255)


                                                                                           ggTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc      <  1:2242912/101‑1 (MQ=255)


                                                                                            gTCGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcg     <  2:7899750/101‑1 (MQ=255)


                                                                                              cGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg   >  2:1658500/1‑101 (MQ=255)


                                                                                              cGATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg   >  2:8605303/1‑101 (MQ=255)


                                                                                               gATG.TCGGGCAG.GGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCgg  >  1:1005242/1‑101 (MQ=255)


TGCCGGGCGACGGGCTTTTGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TC.GGCA..GGGGCGGTGGTC.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGG  >  NC_014034/1769254‑1769446

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 40 ≤


ATCG