Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,823,740 +C 100% intergenic (+25/‑148) RCAP_rcc01687 → / → RCAP_rcc01688 HK97 family phage major capsid protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,823,7401.→C100.0% 148.6 337intergenic (+25/‑148)RCAP_rcc01687/RCAP_rcc01688HK97 family phage major capsid protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (180/109):  ref base (5/43):  total (185/152)
Fisher's exact test strand distribution p-value = 6.14e-12
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.10e-02

Only 100 of 404 total aligned reads displayed.

CTATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.A.CC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTGCGCC  >  NC_014034/1823640‑1823835
                                                                                                        |                                                                                                
ctATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.a.a                                                                                                   >  2:4172112/1‑101 (MQ=255)
ctATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.a.a                                                                                                   >  1:2553328/1‑101 (MQ=255)
 taggCGAGCAAGCGGGTTGGCGGGGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.A.c                                                                                                 <  2:2976795/98‑1 (MQ=255)
 tATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.A.c                                                                                                 >  2:6578113/1‑101 (MQ=255)
  aTGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.A.cc                                                                                                <  2:7941696/101‑1 (MQ=255)
  aTGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.A.cc                                                                                                >  1:6861508/1‑101 (MQ=255)
  aTGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.A.cc                                                                                                >  2:6333384/1‑101 (MQ=255)
      gAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.A.cc.ccgg                                                                                           >  1:8272093/1‑98 (MQ=255)
        gCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGNC.GGGGGCAA.A.CC.CGGCt                                                                                          >  2:3776827/1‑101 (MQ=255)
        gCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.A.cc.ccggct                                                                                         <  2:2238096/101‑6 (MQ=255)
        gCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.A.cc.ccggct                                                                                         <  1:1633104/101‑6 (MQ=255)
        gCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.A.cc.ccggct                                                                                         >  2:2316476/1‑96 (MQ=255)
         cAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGGA.A.A.CC.CGGCTg                                                                                         >  2:5094407/1‑101 (MQ=255)
         cAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTg                                                                                         >  1:8506442/1‑101 (MQ=255)
          aaGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTgc                                                                                        >  1:6282869/1‑101 (MQ=255)
          aaGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTgc                                                                                        >  2:8176260/1‑101 (MQ=255)
           aGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTgcc                                                                                       >  2:4382546/1‑101 (MQ=255)
             cGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTgccgc                                                                                     >  2:6527818/1‑101 (MQ=255)
              gggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTgccgcc                                                                                    >  1:7132637/1‑101 (MQ=255)
              gggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTgccgcc                                                                                    >  1:2247428/1‑101 (MQ=255)
              gggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTgccgcc                                                                                    >  2:3278153/1‑101 (MQ=255)
                  gggCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGtt                                                                                <  1:6564378/101‑1 (MQ=255)
                   ggcggcGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTc                                                                               <  2:256560/101‑1 (MQ=255)
                   ggcggcGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTc                                                                               >  2:6708179/1‑101 (MQ=255)
                   ggcggcGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTc                                                                               <  2:2673079/101‑1 (MQ=255)
                    gcggcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCg                                                                              <  2:8040652/101‑1 (MQ=255)
                     cggcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGc                                                                             >  2:1216908/1‑101 (MQ=255)
                       gcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCg                                                                           >  1:2450330/1‑101 (MQ=255)
                         gATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGt                                                                         >  2:6944797/1‑101 (MQ=255)
                          aTGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTc                                                                        <  2:7622479/101‑1 (MQ=255)
                          aTGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTc                                                                        >  1:1971717/1‑101 (MQ=255)
                           tGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGGTGCCGCCGGTTCGCCGGTcg                                                                       >  1:5642351/1‑101 (MQ=255)
                           tGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTcg                                                                       <  1:3638635/101‑1 (MQ=255)
                              cAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTcgggc                                                                    <  1:279087/101‑1 (MQ=255)
                                gCGATTCCGCTGAAATAAAGCTGTGGAAGTTTGCCCCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTcgggcgg                                                                  <  2:45392/101‑1 (MQ=255)
                                 cGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTcgggcggg                                                                 <  1:401656/101‑1 (MQ=255)
                                  gATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCgggcgggc                                                                >  1:2022146/1‑101 (MQ=255)
                                  gATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCgggcgggc                                                                >  2:3444782/1‑101 (MQ=255)
                                  gATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCgggcgggc                                                                >  1:8393732/1‑101 (MQ=255)
                                   aTTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGggcgggcg                                                               >  1:5775143/1‑101 (MQ=255)
                                         ccgcGATCAAGCTGTTGAAGTTTGCCGCCTCGTGACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCt                                                         >  1:6447715/1‑101 (MQ=255)
                                          cgcgATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCtt                                                        <  1:4969613/101‑1 (MQ=255)
                                           gcgATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGNCGGTTCGCCGGTCGGGCGGGCGCGGGCTTc                                                       >  1:1370374/1‑101 (MQ=255)
                                            cgATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTcc                                                      <  2:2784092/101‑1 (MQ=255)
                                             gATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCg                                                     >  1:823287/1‑101 (MQ=255)
                                               tCAAGCTTTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGc                                                   <  2:6245501/101‑1 (MQ=255)
                                               tCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGc                                                   <  2:2058820/101‑1 (MQ=255)
                                               tCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGc                                                   >  1:1018158/1‑101 (MQ=255)
                                                cAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGcc                                                  >  2:901980/1‑101 (MQ=255)
                                                 aaGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGTTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCg                                                 >  1:4768794/1‑101 (MQ=255)
                                                 aaGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCg                                                 >  2:6118869/1‑101 (MQ=255)
                                                   gCTGTTGAGGTTTGCCGCCTCGTAGCGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCt                                               <  2:8846938/101‑1 (MQ=255)
                                                    cTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTg                                              <  1:7841258/101‑1 (MQ=255)
                                                     tGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGc                                             >  1:8009613/1‑101 (MQ=255)
                                                      gTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGcc                                            >  2:1829927/1‑101 (MQ=255)
                                                       ttGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCt                                           <  1:5189887/101‑1 (MQ=255)
                                                       ttGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCt                                           <  1:568214/101‑1 (MQ=255)
                                                        tGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.ACC.CC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTa                                          <  1:1768938/101‑1 (MQ=255)
                                                           aGTTTGCCCCCTCGTAAGGGGCGGGGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgct                                       <  1:6373405/101‑1 (MQ=255)
                                                            gTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.ACC.CC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctg                                      <  2:5543310/101‑1 (MQ=255)
                                                            gTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.ACC.CC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctg                                      <  1:6201125/101‑1 (MQ=255)
                                                            gTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGGTGCCTAgctg                                      >  1:8102418/1‑101 (MQ=255)
                                                            gTTGGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctg                                      <  2:6858801/101‑1 (MQ=255)
                                                             tttGCCGCCTCGTAACGGGCGGTGAGGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctgc                                     <  1:3204815/101‑1 (MQ=255)
                                                             tttGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctgc                                     >  2:1691052/1‑101 (MQ=255)
                                                               tGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctc                                   >  1:3399764/1‑101 (MQ=255)
                                                               tGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctc                                   <  1:8715065/101‑1 (MQ=255)
                                                                  cgccTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccct                                <  2:4701954/101‑1 (MQ=255)
                                                                   gccTCGTACCGGGCGGTGAAGGGGCC.GGGGGC.ACC.CC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCCTCCGGCCGCTGCCTAGCTGctccctc                               <  2:5774908/101‑1 (MQ=255)
                                                                   gccTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccctc                               <  2:6761274/101‑1 (MQ=255)
                                                                    ccTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccctcc                              >  2:3649039/1‑101 (MQ=255)
                                                                    ccTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccctcc                              <  2:7132333/101‑1 (MQ=255)
                                                                     cTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccg                             >  1:5028603/1‑101 (MQ=255)
                                                                     cTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccg                             >  2:1386097/1‑101 (MQ=255)
                                                                     cTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccg                             <  1:9060419/101‑1 (MQ=255)
                                                                      tCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgt                            >  1:6054482/1‑101 (MQ=255)
                                                                       cGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtc                           >  1:2894918/1‑101 (MQ=255)
                                                                        gTAACGGGCGGTGAAGGGGCC.GGGGGC.ACC.CC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtcc                          <  2:3552494/101‑1 (MQ=255)
                                                                        gTAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtcc                          >  1:6923261/1‑101 (MQ=255)
                                                                         tAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtccg                         >  1:1216213/1‑101 (MQ=255)
                                                                         tAACGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtccg                         >  2:315793/1‑101 (MQ=255)
                                                                          aaCGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGa                        >  2:2521750/1‑101 (MQ=255)
                                                                          aaCGGGCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCACtccgtccgc                        >  2:7362968/1‑100 (MQ=255)
                                                                           aCGGGCGGTGAAGGGGCC.GGGGGC.ACC.CC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAg                       <  1:734125/101‑1 (MQ=255)
                                                                              ggCGGTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTgg                    >  1:2138678/1‑101 (MQ=255)
                                                                                cGGTGAAGGGGCC.GGGGGC.ACC.CC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCg                  <  2:5936325/101‑1 (MQ=255)
                                                                                 ggTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCgg                 >  2:4875085/1‑101 (MQ=255)
                                                                                 ggTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCgg                 >  1:4857540/1‑101 (MQ=255)
                                                                                  ggagaGGGCCC.CGGGGC.ACC.CC.CGGCTCCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGt                <  1:4219737/97‑1 (MQ=255)
                                                                                  gTGAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGt                <  2:8493133/101‑1 (MQ=255)
                                                                                    gAAGGGGCC.GGGGGC.ACC.CC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGc              <  1:1438973/101‑1 (MQ=255)
                                                                                    gAAGGGGCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGGGTGGCGGTGc              >  1:3010446/1‑101 (MQ=255)
                                                                                       ggggCC.GGGGGC.A.ACCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCggg           >  2:5276207/1‑101 (MQ=255)
                                                                                         ggCG.GGGGGC.ACC.CC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGt         <  2:4979130/101‑1 (MQ=255)
                                                                                          gCC.GGGGGC.ACC.CC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTc        <  2:5300865/101‑1 (MQ=255)
                                                                                           gcgggggcC.A.A.CCGCGGCTGCCGCCGGTGCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTc        <  1:4550694/98‑1 (MQ=255)
                                                                                           cc.GGGGGC.ACC.CC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCt       <  2:8618808/101‑1 (MQ=255)
                                                                                            cggggggC.A.GCCC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCt       <  1:7434251/99‑1 (MQ=255)
                                                                                            c.GGGGGC.ACC.CC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTg      <  1:9096575/101‑1 (MQ=255)
                                                                                             ggggggC.A.C.CCACGGTAGCCGCCGTGTCGCCGGTCGGGCGGGCGCGGGGTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTg      <  2:9095271/100‑1 (MQ=255)
                                                                                                        |                                                                                                
CTATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCC.GGGGGC.A.A.CC.CGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTGCGCC  >  NC_014034/1823640‑1823835

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG