Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,843,348 +G 98.4% intergenic (+10/+138) ppiB → / ← tyrS peptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,843,3481.→G98.4% 3.3 ‑1.1 ‑1.8243intergenic (+10/+138)ppiB/tyrSpeptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  new base (21/218):  ref base (1/3):  total (22/221)
Fisher's exact test strand distribution p-value = 3.18e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.70e-02

Only 100 of 289 total aligned reads displayed.

GGATCTGGTCGACAAGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCA.GGG.GGCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GGGGCGCCCTTTCAT  >  NC_014034/1843248‑1843441
                                                                                                     |                                                                                                 
ggATCTGGTCGACAAGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCa                                                                                                    <  2:229195/101‑1 (MQ=255)
      ggTCGACAAGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCA.ggg.ggg                                                                                            >  1:4455488/1‑100 (MQ=255)
       gTCGACAAGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCA.tgg.gccc                                                                                           <  2:8500266/101‑4 (MQ=255)
       gTCGACAAGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCA.ggg.gggc                                                                                           <  1:6800897/101‑3 (MQ=255)
        gcGACAAGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCA.ggg.gggct                                                                                          <  1:9078275/100‑4 (MQ=255)
         cGACAAGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCC.GGG.GGgtcg                                                                                         >  1:5102802/1‑100 (MQ=255)
          gACAAGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCA.ggg.gggctct                                                                                        <  2:9026826/101‑6 (MQ=255)
           aCAAGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTg                                                                                        <  1:2066009/101‑1 (MQ=255)
           aCAAGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTg                                                                                        <  1:411623/101‑1 (MQ=255)
             cttccgatctaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTGcc                                                                                      <  1:4645723/91‑1 (MQ=255)
             aaGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTGcc                                                                                      >  2:3445204/1‑101 (MQ=255)
                  gAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTGcccccct                                                                                 <  2:1936796/101‑2 (MQ=255)
                  gAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTGccaccct                                                                                 >  2:3733419/1‑100 (MQ=255)
                   aaaaaGGGCTCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTGcccccctc                                                                                <  2:8198631/101‑3 (MQ=255)
                   aaaaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTGcccccctc                                                                                <  2:298800/101‑3 (MQ=255)
                    aaaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTGcccccctct                                                                               <  2:4494192/101‑4 (MQ=255)
                    aaaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTGcccccctct                                                                               <  2:8797902/101‑4 (MQ=255)
                    aaaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTGcccccctct                                                                               <  1:329325/101‑4 (MQ=255)
                    aaaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTGcccccctct                                                                               <  2:6638506/101‑4 (MQ=255)
                    aaaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTGcccccctct                                                                               <  2:1198378/101‑4 (MQ=255)
                    aaaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTGcccccctct                                                                               <  1:2263975/101‑4 (MQ=255)
                     aaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTGcccccctctg                                                                              <  1:3757934/101‑5 (MQ=255)
                       aGGGCGGTCCCGATCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTGcccccctctgct                                                                            <  2:7360636/101‑7 (MQ=255)
                          gcgcgcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTGcccccctctgctgcg                                                                         <  1:2999178/101‑10 (MQ=255)
                           cgcgcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTGcccccctctgctgcgc                                                                        <  1:9184375/101‑11 (MQ=255)
                             cgcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCAt                                                                     <  2:5302790/101‑1 (MQ=255)
                              gcgcGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCAtt                                                                    <  2:9124783/98‑1 (MQ=255)
                              gcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCAtt                                                                    <  2:5638596/101‑1 (MQ=255)
                              gcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCAtt                                                                    <  1:3995747/101‑1 (MQ=255)
                              gcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCAtt                                                                    <  1:6131559/101‑1 (MQ=255)
                               cccGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTc                                                                   <  1:2899852/101‑1 (MQ=255)
                               cccGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTc                                                                   <  1:4246468/101‑1 (MQ=255)
                               cccGAGCGGAACGGTCTGTGCGAATCACCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTc                                                                   <  2:2944296/101‑1 (MQ=255)
                                ccGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCa                                                                  <  1:3492216/101‑1 (MQ=255)
                                 cGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCAc                                                                 <  1:8451129/101‑1 (MQ=255)
                                 cGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCAc                                                                 <  1:1670209/101‑1 (MQ=255)
                                   aGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCAccc                                                               <  2:8202055/101‑1 (MQ=255)
                                    gCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCAcccc                                                              <  1:5594802/101‑1 (MQ=255)
                                    gCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCAcccc                                                              <  2:2405791/101‑1 (MQ=255)
                                      tGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCAcccccc                                                            <  2:4806743/101‑1 (MQ=255)
                                      tGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCAcccccc                                                            <  1:7650760/101‑1 (MQ=255)
                                       gAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCg                                                           <  2:6284459/101‑1 (MQ=255)
                                       gAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCg                                                           <  2:8532964/101‑1 (MQ=255)
                                        aaCGGCCGTGTCGTTGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCACCCTCTGCTGCGCATTCACCCCCCgg                                                          <  2:2164420/101‑1 (MQ=255)
                                           ggCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGat                                                       <  2:5912569/101‑1 (MQ=38)
                                           ggCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGat                                                       <  2:7074331/101‑1 (MQ=38)
                                           ggCCTTGGCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGat                                                       <  1:6243077/101‑1 (MQ=37)
                                            gCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGata                                                      <  1:230200/101‑1 (MQ=38)
                                             ccTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGatat                                                     <  2:1020104/101‑1 (MQ=38)
                                              cTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATAtt                                                    <  1:238625/101‑1 (MQ=37)
                                               ttGTCGAAGGCCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATAttt                                                   <  2:4596233/101‑1 (MQ=37)
                                                 gTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTc                                                 <  1:1118095/101‑1 (MQ=37)
                                                 gTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTc                                                 <  2:4199131/101‑1 (MQ=37)
                                                  tCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCg                                                <  1:4352933/101‑1 (MQ=37)
                                                   cGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGGTCTGCCCCCCC.CTGCTGCGCATTCACCCCCCGGGATATTTTCGc                                               >  1:4865555/1‑101 (MQ=37)
                                                    gAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGcc                                              <  1:5716251/101‑1 (MQ=37)
                                                     aaGACGCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCAACCCCCGGCCGATTTTCGCCa                                             >  2:4517508/1‑101 (MQ=255)
                                                     aaGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCa                                             <  1:8688350/101‑1 (MQ=37)
                                                       gACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAg                                           <  2:2588580/101‑1 (MQ=37)
                                                       gACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAg                                           <  2:7808646/101‑1 (MQ=37)
                                                       gACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAg                                           <  2:745966/101‑1 (MQ=37)
                                                       gACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAg                                           <  1:8768067/101‑1 (MQ=37)
                                                          cccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTg                                        <  1:6057308/101‑1 (MQ=37)
                                                          cccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTg                                        <  2:2776749/101‑1 (MQ=37)
                                                           ccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCA.GGGTGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGa                                       <  1:3244197/101‑1 (MQ=37)
                                                            cGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaa                                      <  1:664414/101‑1 (MQ=25)
                                                            cGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaa                                      <  1:1432851/101‑1 (MQ=25)
                                                             gATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaaa                                     <  1:2508458/101‑1 (MQ=25)
                                                             gATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaaa                                     <  1:5457791/101‑1 (MQ=25)
                                                              aTTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAAt                                    <  2:6420768/101‑1 (MQ=25)
                                                              aTTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAAt                                    <  2:5455921/101‑1 (MQ=25)
                                                                tACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGTATTCATCCCCCGGGATATTTTCGCCAAGGTGAAATgg                                  >  2:1700437/1‑101 (MQ=25)
                                                                tACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATgg                                  <  2:4769284/101‑1 (MQ=25)
                                                                  cATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaa                                <  2:3547137/101‑1 (MQ=25)
                                                                   aTCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaa                               >  2:1951166/1‑101 (MQ=25)
                                                                   aTCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaa                               <  2:7914629/101‑1 (MQ=25)
                                                                   aTCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaa                               <  2:322881/101‑1 (MQ=25)
                                                                     cGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTATG.CCCCCCTCTGCTGCGCATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAg                             <  1:1577747/101‑1 (MQ=25)
                                                                       tCAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAggg                           <  2:6884935/101‑1 (MQ=25)
                                                                        cAAGGCCACGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGc                          >  1:7154790/1‑101 (MQ=25)
                                                                              cACGATCGATTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctccg                    <  2:4255632/101‑6 (MQ=21)
                                                                                cGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCt                  <  1:5946112/101‑1 (MQ=25)
                                                                                cGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCt                  <  1:1377896/101‑1 (MQ=21)
                                                                                cGATCGAGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCt                  <  1:1293547/101‑1 (MQ=21)
                                                                                      aGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggc           <  2:2664107/101‑3 (MQ=21)
                                                                                      aGTGACACGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggc           <  1:8917874/101‑3 (MQ=21)
                                                                                         gACTCGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCGCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggcgcc        <  1:736774/101‑6 (MQ=18)
                                                                                          acacGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTGACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGccc        <  2:4002908/101‑1 (MQ=21)
                                                                                          acacGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGccc        <  1:837667/101‑1 (MQ=21)
                                                                                            acGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCtt      <  2:8010997/101‑1 (MQ=21)
                                                                                             cGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCttt     <  2:2489967/101‑1 (MQ=21)
                                                                                             cGGGCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCttt     <  2:1283725/101‑1 (MQ=21)
                                                                                             cGGGCGCAGGGG.GGCCCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCttt     <  2:4667971/101‑1 (MQ=18)
                                                                                              gggCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTc    <  2:7701243/101‑1 (MQ=21)
                                                                                              gggCGCAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTc    <  1:1815725/101‑1 (MQ=21)
                                                                                               ggCGCAGGGG.GGCTTTG.CCCCCCTCTGCTGCTCATTCTCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCa   <  1:3694039/101‑1 (MQ=19)
                                                                                                gcgcAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCAt  <  1:6890916/101‑1 (MQ=21)
                                                                                                gcgcAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCAt  <  1:8171598/101‑1 (MQ=21)
                                                                                                gcgcAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCAt  <  1:1005989/101‑1 (MQ=21)
                                                                                                gcgcAGGGG.GGCTCTG.CCCCCCTCTGCTGCGCAGTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCAt  <  1:6971501/101‑1 (MQ=21)
                                                                                                     |                                                                                                 
GGATCTGGTCGACAAGCTGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCA.GGG.GGCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GGGGCGCCCTTTCAT  >  NC_014034/1843248‑1843441

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG