Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,843,369 +C 100% intergenic (+31/+117) ppiB → / ← tyrS peptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,843,3691.→C100.0% 836.8 264intergenic (+31/+117)ppiB/tyrSpeptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  new base (41/223):  ref base (0/0):  total (41/223)

Only 100 of 283 total aligned reads displayed.

CGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.A.GGGGGCTCTG.CCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GGGGCGCCCTTTCATCTTGCCACAAATATCCCGGG  >  NC_014034/1843275‑1843461
                                                                                                  |                                                                                              
   gcgcGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCAtt                                                                                        <  2:9124783/98‑1 (MQ=255)
   gcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCAtt                                                                                        <  2:5638596/101‑1 (MQ=255)
   gcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCAtt                                                                                        <  1:3445308/101‑1 (MQ=255)
   gcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG...CCCCCTCTGCTGCGCATTc                                                                                       <  2:7447880/101‑1 (MQ=255)
   gcCCGAGCTGAACGGCCTTGGCGAAGCCCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCAtt                                                                                        <  1:4421264/101‑1 (MQ=255)
    cccGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTc                                                                                       <  1:5652443/101‑1 (MQ=255)
    cccGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTc                                                                                       <  1:2899852/101‑1 (MQ=255)
      cGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCAc                                                                                     <  2:1117267/101‑1 (MQ=255)
      cGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCAc                                                                                     <  1:598730/101‑1 (MQ=255)
         gCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCAcccc                                                                                  <  1:5594802/101‑1 (MQ=255)
          cTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCAccccc                                                                                 <  2:298283/101‑1 (MQ=255)
          cTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCAccccc                                                                                 <  2:4582826/101‑1 (MQ=255)
           ttaaCGGCCTTGTCGAAGTCCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCAcccccc                                                                                <  2:4510527/99‑1 (MQ=255)
           tGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGTCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCAcccccc                                                                                <  2:4775887/101‑1 (MQ=255)
           tGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCAcccccc                                                                                <  2:4751361/101‑1 (MQ=255)
            gAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCg                                                                               >  1:1604538/1‑101 (MQ=255)
             aaCGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCgg                                                                              <  2:2116345/101‑1 (MQ=255)
                ggCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGat                                                                           <  2:8083501/101‑1 (MQ=38)
                ggCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGat                                                                           <  2:5912569/101‑1 (MQ=38)
                ggCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGat                                                                           <  2:7074331/101‑1 (MQ=38)
                  gcTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCGCCCCCCGGGatat                                                                         <  1:8448211/100‑1 (MQ=255)
                  ccTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGatat                                                                         <  1:3262568/101‑1 (MQ=38)
                   cTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATAtt                                                                        <  1:238625/101‑1 (MQ=37)
                    ttGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGGTAttt                                                                       <  2:8321632/101‑1 (MQ=37)
                       tCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCg                                                                    <  1:7944005/101‑1 (MQ=37)
                       tCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCg                                                                    <  2:8504427/101‑1 (MQ=37)
                       tCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCg                                                                    <  1:4352933/101‑1 (MQ=37)
                        cGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAA.GGGGGGCCTGCCCCCCCCTCCC.TGCCACTTCCCCCCCCGGG.TAtttttcc                                                                   >  1:610979/1‑98 (MQ=255)
                        cGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGGTCTG.CCCCCCC.CTGCTGCGCATTCACCCCCCGGGATATTTTCGc                                                                   >  1:4865555/1‑101 (MQ=37)
                        cGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGGCCCG.GCCCCCCCCGC.CGGGCACTCACCCCCCgggggcgttttcc                                                                   >  1:5789655/1‑91 (MQ=255)
                         gAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATACTTTCGcc                                                                  <  1:8680360/101‑1 (MQ=37)
                          aaGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGGTCTG.CCCCCCCCTCC.TGCGCCTTCACCCCCCCGGATAGTTTCGCCa                                                                 >  2:3690403/1‑101 (MQ=255)
                            gACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAg                                                               <  1:8768067/101‑1 (MQ=37)
                             aCCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGGTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCCGGATCTTTTCGCCAAgg                                                              >  1:3475502/1‑101 (MQ=255)
                               cccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTg                                                            <  1:3799981/101‑1 (MQ=37)
                                 cGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaa                                                          <  1:7519692/101‑1 (MQ=25)
                                 cGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaa                                                          <  2:4093768/101‑1 (MQ=25)
                                 cGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaa                                                          <  1:664414/101‑1 (MQ=25)
                                 cGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaa                                                          <  1:244735/101‑1 (MQ=25)
                                 cGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaa                                                          <  1:1432851/101‑1 (MQ=25)
                                  gATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaaa                                                         <  1:5457791/101‑1 (MQ=25)
                                  gATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaaa                                                         <  1:404074/101‑1 (MQ=25)
                                  gATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaaa                                                         <  1:1553581/101‑1 (MQ=25)
                                   aTTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAAt                                                        <  2:5455921/101‑1 (MQ=25)
                                     ttcATCGGCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATgg                                                      <  1:4213648/99‑1 (MQ=25)
                                     tACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATgg                                                      <  1:6549058/101‑1 (MQ=25)
                                      aCATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGa                                                     <  2:5102633/101‑1 (MQ=25)
                                      aCATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGa                                                     <  1:6145803/101‑1 (MQ=25)
                                       cATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaa                                                    <  2:3505436/101‑1 (MQ=25)
                                       cATCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaa                                                    <  2:7470976/101‑1 (MQ=25)
                                        aTCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGGTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCCCGGAATTTTCGCCAAGGGGAAATGGgaa                                                   >  2:1522981/1‑101 (MQ=255)
                                        aTCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaa                                                   <  2:322881/101‑1 (MQ=25)
                                        aTCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaa                                                   <  2:7914629/101‑1 (MQ=25)
                                        aTCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCATCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaa                                                   >  2:1030196/1‑101 (MQ=25)
                                         tCGTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaaa                                                  <  2:8199361/101‑1 (MQ=25)
                                         gcGTCCAGGACACGATCGAGTGACACGGGCGC.AGGGGGGCGCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaaa                                                  <  2:2549116/100‑1 (MQ=18)
                                           gTCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAgg                                                <  2:46167/101‑1 (MQ=25)
                                            tCAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAggg                                               <  2:8719598/101‑1 (MQ=25)
                                             cAAGGCCACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGc                                              >  1:7154790/1‑101 (MQ=25)
                                                  ccACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctcc                                         <  2:2475686/101‑5 (MQ=25)
                                                  ccACGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctcc                                         <  2:2856892/101‑5 (MQ=25)
                                                   cACGATCGATTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctccg                                        <  2:4255632/101‑6 (MQ=21)
                                                     cGATCGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCt                                      <  1:1377896/101‑1 (MQ=21)
                                                         cGAGTGATACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggg                                 <  2:7806626/101‑1 (MQ=21)
                                                         cGAGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggg                                 <  1:508056/101‑1 (MQ=21)
                                                           aGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggc                               <  1:8917874/101‑3 (MQ=21)
                                                           aGTGACACGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggc                               <  2:2664107/101‑3 (MQ=21)
                                                              gACTCGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCGCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggcgcc                            <  1:736774/101‑6 (MQ=18)
                                                               gcacGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGccc                            <  2:1944618/100‑1 (MQ=21)
                                                               acacGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTGACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGccc                            <  2:4002908/101‑1 (MQ=21)
                                                               acacGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGccc                            <  2:6760073/101‑1 (MQ=21)
                                                               acacGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGccc                            <  1:837667/101‑1 (MQ=21)
                                                                cacGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCt                           <  1:9145078/101‑1 (MQ=21)
                                                                  cGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCttt                         <  2:1283725/101‑1 (MQ=21)
                                                                  cGGGCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCttt                         <  1:1242114/101‑1 (MQ=21)
                                                                  cGGGCGC.AGGGGGGCGCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCttt                         <  1:2302272/101‑1 (MQ=18)
                                                                   gggCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTc                        <  1:1700484/101‑1 (MQ=21)
                                                                   gggCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTc                        <  1:1815725/101‑1 (MQ=21)
                                                                   gggCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTc                        <  1:2186964/101‑1 (MQ=21)
                                                                   gggCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTc                        <  2:7701243/101‑1 (MQ=21)
                                                                   gggCGC.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTc                        <  2:2405248/101‑1 (MQ=21)
                                                                    ggCGC.AGGGGGGCTTTG..CCCCCCTCTGCTGCTCATTCTCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCa                       <  1:3694039/101‑1 (MQ=19)
                                                                     gcgc.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCAt                      <  1:1005989/101‑1 (MQ=21)
                                                                     gcgc.AGGGGGGCTCTG..CCCCCCTCTGCTGCGCAGTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCAt                      <  1:6971501/101‑1 (MQ=21)
                                                                        gcaggggggCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCt                    <  2:6145990/97‑1 (MQ=21)
                                                                           ggggggCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGc                 >  1:6306097/2‑101 (MQ=21)
                                                                           ggggggCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGc                 <  2:6934491/100‑1 (MQ=21)
                                                                           ggggggCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGc                 <  1:1110075/100‑1 (MQ=21)
                                                                            gggggCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGcc                >  2:1866927/1‑101 (MQ=21)
                                                                            gggggCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGcc                >  2:3348397/1‑101 (MQ=21)
                                                                               ggCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCcaca             <  2:4898736/101‑1 (MQ=25)
                                                                                gCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaa            >  1:3076313/1‑101 (MQ=25)
                                                                                gCTCTG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaa            <  1:2519531/101‑1 (MQ=25)
                                                                                 ctctG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaaa           >  2:6572176/1‑101 (MQ=25)
                                                                                 ctctG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaaa           >  1:7745567/1‑101 (MQ=25)
                                                                                 ctctG..CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaaa           >  1:8219716/1‑101 (MQ=25)
                                                                                    tctgccccccTCTGCTGCGCATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAat          <  2:6407299/97‑1 (MQ=25)
                                                                                     ctgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAatc         >  1:3631027/4‑100 (MQ=25)
                                                                                      tgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAatat        <  2:2184747/99‑1 (MQ=25)
                                                                                       gccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATc       <  1:6852161/100‑1 (MQ=37)
                                                                                                  |                                                                                              
CGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGC.A.GGGGGCTCTG.CCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GGGGCGCCCTTTCATCTTGCCACAAATATCCCGGG  >  NC_014034/1843275‑1843461

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG