Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,867,833 +C 100% intergenic (‑235/+80) RCAP_rcc01729 ← / ← RCAP_rcc01730 pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,867,8331.→C100.0% 47.0 213intergenic (‑235/+80)RCAP_rcc01729/RCAP_rcc01730pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase
Reads supporting (aligned to +/- strand):  new base (83/113):  ref base (0/17):  total (83/130)
Fisher's exact test strand distribution p-value = 1.66e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.19e-01

Only 100 of 359 total aligned reads displayed.

GAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CG.C.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCAA  >  NC_014034/1867733‑1867928
                                                                                                       |                                                                                                   
 aaagGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCCGCCCCCCCGGCGCGA.AAg.cg.c                                                                                                    >  1:7228357/1‑101 (MQ=37)
 aaaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAg.cg.c                                                                                                    >  1:1849241/1‑101 (MQ=255)
 aaaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCCCCCCCCCCGGCGCGA.AAg.cg.c                                                                                                    >  1:2395189/1‑101 (MQ=37)
   aaGCACTTCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.cg.c.cc                                                                                                 <  2:6945992/101‑2 (MQ=255)
   aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.cg.c.cc                                                                                                 <  1:4628952/101‑2 (MQ=255)
   aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.cg.c.cc                                                                                                 <  1:7917447/101‑2 (MQ=255)
    aGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.cg.c.ccg                                                                                                <  2:2578442/101‑3 (MQ=255)
    aGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.cg.c.cca                                                                                                >  2:3769928/1‑99 (MQ=255)
    aGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.ag.c.ccc                                                                                                >  2:4035051/1‑99 (MQ=255)
    aGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGG.CAG.cg.c.ccc                                                                                                >  1:2875203/1‑99 (MQ=255)
       aCTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGGGCGC.AAA.cg.c.ccgccc                                                                                             >  2:1255415/1‑96 (MQ=37)
         tGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGA.AAG.cg.c.ccgccctg                                                                                           >  1:5266033/1‑94 (MQ=37)
          gCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCCGCCCCCGCGGCGCGA.AAG.cg.c.ccgccacgg                                                                                          >  2:6526280/1‑93 (MQ=25)
           cTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGA.AAG.Cg.c.cccccctggg                                                                                         >  1:7469807/1‑95 (MQ=37)
            ttttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGGCCGCACCCCCGCCGCCGCGC.AAG.Cg.c.cccccccggcc                                                                                        >  1:2952468/1‑94 (MQ=25)
            ttttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAGGCCCCGCCCCCGCGGCTCGA.AAG.Cg.c.cccccctgggg                                                                                        >  1:5608283/1‑94 (MQ=25)
            ttttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCCCCCCCCGCGGCGCGA.AAG.Cg.c.ccccccccggc                                                                                        >  1:4048121/1‑94 (MQ=25)
              ttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCCC.AAA.CGCC.CCCCACgggcgg                                                                                       >  1:165911/1‑101 (MQ=25)
              ttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAAGCCCAGCCCCCGCGGCGCGC.AAG.Cg.c.ccccccagggcgg                                                                                      >  1:2700811/1‑92 (MQ=255)
              ttCCACGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCACgggcgg                                                                                       >  2:7905923/1‑101 (MQ=21)
               tCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCACCCCCCGCGGCGCGA.AAA.Cg.c.cccccccgggcggg                                                                                     >  1:2588419/1‑91 (MQ=25)
                ccccGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCTCGA.AAG.CGCC.CGCCATgggcgggg                                                                                     <  2:4383742/101‑1 (MQ=255)
                    gcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGgcggggc.ggg                                                                                <  2:1492293/101‑1 (MQ=255)
                    gcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGA.AAG.CGCC.CGCCCTGGgcggggc.ggg                                                                                >  1:102573/1‑101 (MQ=21)
                    gcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAGGCCCCGGCCCCGCGGCGCGG.AAA.CG.CGCGCCCCGGgcggggg.ggg                                                                                >  1:7807789/1‑101 (MQ=255)
                    gcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGC.AAG.AGCC.CCCCATGGgcggggg.ggg                                                                                >  2:263940/1‑101 (MQ=255)
                       cgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGgcgc                                                                             <  1:7533737/101‑1 (MQ=255)
                       cgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGAGCCCCCCCCCCGCGGCGCGC.AAA.CGCC.CGCCATGGGGGGGGC.GGgggc                                                                             >  1:7609516/1‑101 (MQ=255)
                        gtgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAAGCCCAGCCCCGGCGGCGCGG.AAA.CGCC.CGCCATGGGCGGGGC.GGgggcc                                                                            >  1:3245450/1‑100 (MQ=21)
                         tgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGgcgccg                                                                           >  1:7348370/1‑99 (MQ=255)
                               gACAGCGTTGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.g                                                                   <  2:8343406/101‑1 (MQ=255)
                               ccgggCGGTGCCAAGACCGTTTCCCCAGGCGCAGCAGAGGCCGGCGCCC.GCCCCGGCGG.GCGAGAAG.CGCC.CGCCATGGGCGGGGC.GGGGGCGGGGC.GC.gg                                                                  >  2:4943987/5‑101 (MQ=18)
                                    cGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGCGGGGGGCGGGCC.GG.GGGcc                                                               >  1:3601163/1‑101 (MQ=17)
                                     gTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGGGCCGGGC.CA.GGGCCtt                                                             >  1:4546003/1‑101 (MQ=18)
                                     gTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCCGGCC.GC.ggggcct                                                             >  1:618073/1‑97 (MQ=17)
                                      ttGCAAACACCGCCTCCCAAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCttt                                                            <  2:7348046/101‑1 (MQ=17)
                                        gCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCCGGGC.GA.GGGCCctttg                                                          >  2:7622219/1‑99 (MQ=17)
                                         cAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCCGGCC.GAGGGGCCTTTgg                                                          >  1:2098986/1‑101 (MQ=17)
                                            agcccGCAGCCCCAAGCGGGCGGGAGCCCGGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGcc                                                      <  1:7366659/98‑1 (MQ=14)
                                            agcccGCACCCCCAAGCGCGCGAGAGGCCGGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGcc                                                      <  1:1350824/98‑1 (MQ=17)
                                            aGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCCGGGC.CA.GGGCCCTTGGGGcc                                                      >  1:5710906/1‑101 (MQ=17)
                                            aGACCGCACCCCCAGGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGcc                                                      <  1:3064577/101‑1 (MQ=17)
                                             gcccGCATCCCCAGGCGCGCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGccc                                                     <  1:6742398/99‑1 (MQ=17)
                                               ccccacccccAAGCGCGCGAGGGGCCGGCGCCCAGCCCGGGCGGCGCGA.ACG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGccccc                                                   <  2:5014647/95‑1 (MQ=14)
                                               ccGCCTCCCCAACCGCGCGAGAGCCCAGCCCCCAGCCCCGGCGGCGCGA.AAGCCC.C.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGccccc                                                   <  2:4229418/101‑1 (MQ=17)
                                               ccGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCGGGGC.GA.GGGGCCTTGGGGgcccc                                                   >  2:606385/1‑101 (MQ=17)
                                                cGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCcg                                                  <  2:2453407/101‑1 (MQ=255)
                                                cGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCCGGCC.CAGGGGCCTTTGGGGccccc                                                   >  2:7459849/1‑101 (MQ=17)
                                                cGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGA.AAA.CGCC.CGCCCTGGGCGGGGC.GGGCGCCGGCC.CAGGGGCCTTTGGGGccccg                                                   >  2:7544814/1‑100 (MQ=17)
                                                 gCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCCTGGGCGGGGG.GGGGGCCGGCC.GAGGGGCGTTTGGGGCCCCcg                                                  >  2:8212263/1‑101 (MQ=17)
                                                  cATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCCGGGC.GAGGGGCTTTTGGGGCCCCcgg                                                 >  1:5062971/1‑101 (MQ=17)
                                                  cATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGA.AAG.CGCC.CGCCCTGGGCGGGGG.GGGGGCCGGCC.GC.GGGCCCTTGGGGGCCCccgc                                                >  2:1176146/1‑101 (MQ=17)
                                                   aTCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCCA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCGGGGC.GAGGGGCCTTGGGGGCCCCcgcg                                                >  2:2893548/1‑99 (MQ=17)
                                                    tCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGGGGGGC.GGGGGCGGGGC.GC.GGGCCCTTGGGGGCCCccgcgg                                              >  1:3777007/1‑101 (MQ=17)
                                                      cccAGGCGCGCGAGGGGCCAGCGCCCAGCCCGGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTc                                            <  2:1950187/101‑1 (MQ=17)
                                                       ccAAGGGCGCGAGAGCCCAGCGCCCAGCCCGGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCa                                           <  2:591928/101‑1 (MQ=17)
                                                       ccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCCGGCCGGC.GGGCCTTTGGGGCCCCcgccgtca                                            >  2:1696370/1‑98 (MQ=17)
                                                       caaggccggcgAGGGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCa                                           <  2:5496728/93‑1 (MQ=14)
                                                         aaGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GAGGGGCCTTTGGGG.CCCCGGCGGGCAgg                                         >  2:5813560/1‑101 (MQ=17)
                                                         aaGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCCGGGC.GAGGGGCCTTTGGGGCCCCC.GCGGGCAgg                                         >  2:4056370/1‑101 (MQ=17)
                                                             gcgcgAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGa                                     <  2:2680858/101‑1 (MQ=255)
                                                             ccgcgAGGGGCTGGCGCCCGGCCCGGGGGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGa                                     <  1:8862055/100‑1 (MQ=14)
                                                               gcgAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGGCCCCCGCGGGCCGGGGGGTc                                   >  2:3018669/1‑101 (MQ=14)
                                                               gcgAGAGCCCGGCCCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGTGGATc                                   <  2:6228154/101‑1 (MQ=17)
                                                                 gagaGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCCTTGGGGGCCCCGGCGGGCCGGGGGATCTg                                 >  2:3907581/1‑101 (MQ=17)
                                                                  agagGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGGGCCGGCC.GA.GGGCCTTTTGGGCCCCCGGCGGGCACGGGGGTCTGt                                >  2:2249990/1‑101 (MQ=14)
                                                                   ggggCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                               <  2:4545868/99‑1 (MQ=255)
                                                                   gagGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                               <  2:6422246/101‑1 (MQ=255)
                                                                    gggccgccccccAGCCCCGGCGCCGCGA.AAGCCC.C.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTg                              <  2:3260394/92‑1 (MQ=17)
                                                                    agGCCAGCGCCCGGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTg                              <  1:5410368/101‑1 (MQ=255)
                                                                    agGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTg                              >  1:8377249/1‑101 (MQ=255)
                                                                     ggCCAGCGCCCGGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTgc                             <  2:3601055/101‑1 (MQ=255)
                                                                     ggCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGGTCTGTTgc                             >  1:2778723/1‑101 (MQ=255)
                                                                      gCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTgcg                            >  1:7022183/1‑101 (MQ=255)
                                                                        cAGCGCCCAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCa                          >  2:1015753/1‑101 (MQ=255)
                                                                         ggcccccAGCCCCGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCAt                         <  1:4203016/97‑1 (MQ=255)
                                                                          gcccccGGCCCGGGCGGCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATg                        <  2:8852472/98‑1 (MQ=18)
                                                                          gcccccGGCCCGGGCGGCGCAA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATg                        <  2:8788410/98‑1 (MQ=18)
                                                                          gcccccAGCCGCGGCGGCCCAA.AAGCCC.C.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATg                        <  2:8462520/98‑1 (MQ=18)
                                                                             cccAGCCCCGGCGGCGCGA.AGGCCC.C.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGcc                     <  2:6701875/101‑1 (MQ=18)
                                                                             cccAGCCCCGGCGGCGCGA.ACG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGcc                     <  2:2944984/101‑1 (MQ=255)
                                                                                  ccccGGCGGGGCGA.AAC.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTc                <  1:353247/101‑1 (MQ=18)
                                                                                  ccccGGCGGCGGGA.ACGCCC.C.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTc                <  2:7709547/101‑1 (MQ=18)
                                                                                  ccccGGCGGCGCGA.AGGCCC.C.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTc                <  2:7180819/101‑1 (MQ=18)
                                                                                  ccccGGCGGCCCGG.AAG.CGCC.CGCTATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGGCAGGGGGATCTGTTGCGCCTGGCcgcgcc                >  2:3115187/1‑99 (MQ=18)
                                                                                   ccggggggcgGGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa               <  1:6777289/95‑1 (MQ=18)
                                                                                   ccgggcggcGCGA.AAGCCC.C.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa               <  1:8599741/98‑1 (MQ=21)
                                                                                   ccgggcgccGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa               <  2:5597060/98‑1 (MQ=21)
                                                                                     cggcggCGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCGTGGCCGCGTCAgc             >  1:15798/1‑101 (MQ=255)
                                                                                      ggcggcGCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAgcg            >  1:2625389/1‑101 (MQ=255)
                                                                                        cggcgCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGcgcc          >  2:8509117/1‑101 (MQ=255)
                                                                                        cggcgCGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGcgcc          >  2:5222056/1‑101 (MQ=255)
                                                                                          gcgcGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGcgccgc        >  2:3354270/1‑101 (MQ=255)
                                                                                          gcgcGA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGcgccgc        >  1:8730524/1‑101 (MQ=255)
                                                                                           cgcgA.AGG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCcgcg       <  2:7047553/101‑1 (MQ=255)
                                                                                            gcgA.AAG.CGCC.CGCCATGGGCGGGGC.GGCCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCgg      >  1:6530863/1‑101 (MQ=255)
                                                                                             cgA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCCGCGCCGCGGc     >  2:8982442/1‑101 (MQ=255)
                                                                                              gA.AAG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGGCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGcc    >  1:4233680/1‑101 (MQ=255)
                                                                                                 aaG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCaa  <  2:8041277/101‑1 (MQ=255)
                                                                                                 aaG.CGCC.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCaa  >  2:7265545/1‑101 (MQ=255)
                                                                                                       |                                                                                                   
GAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AAG.CG.C.CGCCATGGGCGGGGC.GGGCGCTGGCC.GA.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCAA  >  NC_014034/1867733‑1867928

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG