Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,927,209 +C 1.8% intergenic (+48/‑125) RCAP_rcc01780 → / → yajC M20 family peptidase/preprotein translocase subunit YajC

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,927,2091.→C 1.8% 6.6 ‑2.8 ‑0.0285intergenic (+48/‑125)RCAP_rcc01780/yajCM20 family peptidase/preprotein translocase subunit YajC
Reads supporting (aligned to +/- strand):  new base (0/5):  ref base (135/145):  total (135/150)
Fisher's exact test strand distribution p-value = 6.20e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 368 total aligned reads displayed.

TCGGCGCCAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGAAAACCGGGGCAACGCCCCA  >  NC_014034/1927109‑1927309
                                                                                                     |                                                                                                    
tCGGCGCCAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCttttt                                                                                                       <  2:2005603/101‑1 (MQ=255)
tCGGCGCCAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCttttt                                                                                                       <  1:2074871/101‑1 (MQ=255)
tCGGCGCCAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCttttt                                                                                                       <  2:3378159/101‑1 (MQ=255)
tCGGCGCCAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCttttt                                                                                                       <  1:5837488/101‑1 (MQ=255)
 cGGCGCCAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGGGCAGCCGAAGAGGGGGGGGCGCCCCCCTTTT.c                                                                                                     >  1:4972319/1‑101 (MQ=255)
 cGGCGCCAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGGGGCGCCCCCTTTTT.c                                                                                                     >  2:2408985/1‑101 (MQ=255)
  ggCGCCAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGGCTTCGGCGCAGCCGAAGAGGGGGGGGCGCCCCCttttttc                                                                                                     >  2:7943414/1‑99 (MQ=255)
   gcgcCAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCt                                                                                                   <  1:1009315/101‑1 (MQ=255)
   gcgcCAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCt                                                                                                   <  1:7952954/101‑1 (MQ=255)
    cgccgccttcttTGCGCGGCTGGTCGGTCGCGCGCTTCCGGCCCGCTGAGGCGTGGGGTCTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTg                                                                                                  <  2:7306039/95‑1 (MQ=255)
    cgcCAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTg                                                                                                  <  2:2834078/101‑1 (MQ=255)
    cgcCAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTg                                                                                                  <  1:9086534/101‑1 (MQ=255)
    cgcCAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTg                                                                                                  <  1:3710135/101‑1 (MQ=255)
      ccAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGTCTTCGGCGCCGCCGAAGGGGGGGCGGGGGCCCCTTTTT.CCTgcc                                                                                                >  2:5299058/1‑99 (MQ=255)
       cAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.TCTGaca                                                                                               >  2:4358628/1‑101 (MQ=255)
       cAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGaca                                                                                               >  1:6223629/1‑101 (MQ=255)
       cAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGaca                                                                                               <  1:4876363/101‑1 (MQ=255)
        aGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGacac                                                                                              <  1:6432818/101‑1 (MQ=255)
        aGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGacac                                                                                              <  2:1661140/101‑1 (MQ=255)
        aGCTTCGTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGacac                                                                                              <  1:3261654/101‑1 (MQ=255)
         gCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGAcaca                                                                                             <  2:7624849/101‑1 (MQ=255)
         gCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGAcaca                                                                                             >  1:3780231/1‑101 (MQ=255)
          cttcttTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGGCTTCGGCGCAGCCGAAGAGGGGGGGGCGGCCCCCTTTT.CCTGaccaaa                                                                                            >  1:7700638/1‑97 (MQ=255)
           ttcttTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAAc                                                                                           <  1:7666049/101‑1 (MQ=255)
           ttcttTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAAc                                                                                           >  1:8638885/1‑101 (MQ=255)
            tcttTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAAcc                                                                                          >  1:5525979/1‑101 (MQ=255)
             cttTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGGCGAAGAGGGGGGGGCGCCCCCCTTTT.CCTCACACAACCg                                                                                         >  2:2891790/1‑101 (MQ=255)
             cttTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCg                                                                                         >  1:7583629/1‑101 (MQ=255)
             cttTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCg                                                                                         >  2:7739888/1‑101 (MQ=255)
             cttTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCg                                                                                         <  1:3138911/101‑1 (MQ=255)
              ttgtcccGCCGGGTCGAGCGCGCGCTTCCGCCCCGCTGAGGCGGGGGGACTTCGGCGCAGCGGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGt                                                                                        <  2:2921813/97‑1 (MQ=255)
               ttGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTg                                                                                       <  1:8970112/101‑1 (MQ=255)
               ttGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCCTTTT.CCTGACACAAACGTg                                                                                       >  1:7627972/1‑101 (MQ=255)
                tGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGGCTTCGGCGCAGCCGAAGAGGGGGGGGCGCCCCCTTTTT.CCTGACCCCAccccga                                                                                      >  1:4282731/1‑97 (MQ=255)
                  ccgggCTGGTCGGCCGCGCGCTCCCGGCCCGCGGGGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATc                                                                                    <  1:7293928/98‑1 (MQ=255)
                  ccgggCGGGTCGAGCGCGCGCGTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATc                                                                                    <  1:3949508/98‑1 (MQ=255)
                  cccGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATc                                                                                    <  2:890420/101‑1 (MQ=255)
                  cccGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATc                                                                                    <  2:3111025/101‑1 (MQ=255)
                   ccGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATcg                                                                                   <  2:6346375/101‑1 (MQ=255)
                    cGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGGCTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATcgc                                                                                  >  2:550043/1‑101 (MQ=255)
                    cGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGGGGCGCCCCCCTTTT.CCTGACACAACCGTGATcgc                                                                                  >  2:1536167/1‑101 (MQ=255)
                     ggCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATcgcg                                                                                 >  1:1054499/1‑101 (MQ=255)
                      gCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGGCTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCgg                                                                                >  1:344420/1‑101 (MQ=255)
                      gCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGCCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCgg                                                                                <  1:4385650/101‑1 (MQ=255)
                       cTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGGCTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCCGc                                                                               >  2:3243612/1‑101 (MQ=255)
                         ggTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGgcc                                                                             >  2:3750901/1‑101 (MQ=255)
                         ggTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGccc                                                                             >  1:1690477/1‑101 (MQ=255)
                          gTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGcccc                                                                            >  2:4166162/1‑101 (MQ=255)
                           tCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCg                                                                           <  1:2661157/101‑1 (MQ=255)
                            cGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGGCTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCgg                                                                          >  2:3453131/1‑101 (MQ=255)
                            cGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCgg                                                                          >  2:1398121/1‑101 (MQ=255)
                            cGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAAACGGGATCGCGGGCCCgg                                                                          >  2:6721177/1‑101 (MQ=255)
                             gAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGTCGTTTGGGCGGGCTAACAGGGGGCGGCGGCCCCCTTTT.CCCTACTCAACATGGCTCGCGGGCCCCGc                                                                         >  2:8583340/1‑101 (MQ=255)
                             gAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGc                                                                         >  2:920844/1‑101 (MQ=255)
                               gcgcgcgcTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCt                                                                       <  2:8330940/101‑1 (MQ=255)
                                 gcgcgcTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGGGCCCCCCTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCCGc                                                                     >  1:3306632/1‑101 (MQ=255)
                                 gcgcgcTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGc                                                                     <  1:8125952/101‑1 (MQ=255)
                                  cgcgcTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGcc                                                                    >  1:2396632/1‑101 (MQ=255)
                                  cgcgcTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGcc                                                                    <  1:7131111/101‑1 (MQ=255)
                                                gCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCCCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGcc                                                      <  1:5152302/101‑1 (MQ=255)
                                                  tGAGGCGGGGGGACTTCGGCCCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCAt                                                    <  1:525209/101‑1 (MQ=255)
                                                   gtggCGGTGGTACTTCGGCTCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCAtt                                                   <  2:8123087/99‑1 (MQ=255)
                                                   ggggtgggggcACTGCGGGCCAGCGGAAGGGGGGGCGGCGCCCCTTTTTT.CCTGACACAACCGGGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCAtt                                                   <  2:7353648/96‑1 (MQ=255)
                                                   gcggCGGGGGGCCTTCGGCCCAGCCGAAGAGGGGGCGCCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCAtt                                                   <  2:509317/99‑1 (MQ=255)
                                                   gAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCCtt                                                   >  1:8720694/1‑101 (MQ=255)
                                                    gggCGGGGGGACTTCGGCGCAGCCGAAGGGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTg                                                  <  1:2280342/100‑1 (MQ=255)
                                                      gCGGGGGGCCTTCGGCGCAGCCGAAGAGGGGGCGGCCCCCCCTTTTC.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCg                                                <  2:1040721/101‑1 (MQ=255)
                                                      gCGGGGGCACTTGGGCGCAGCCGAGGAGGGGGCGGCTCCCCCTTTTC.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCg                                                <  2:3863317/101‑1 (MQ=255)
                                                           gggCCTTGGCCGCGGCCGAAGGGGGGGCGGCCCCCCCTTTTC.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCgggggg                                           <  2:6531089/101‑1 (MQ=255)
                                                           gggACTTCGGCGCAGCCGAAGAGGGGGCGGCCCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCgggggg                                           <  2:5213394/101‑1 (MQ=255)
                                                              ccTTCGGCGCAGCCGAAGGGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGgcg                                        <  2:6124470/100‑1 (MQ=255)
                                                               cTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGgcgc                                       >  2:4435559/1‑101 (MQ=255)
                                                                  cGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGc                                    >  2:708429/1‑101 (MQ=255)
                                                                    gggcggCCGAGGGGGGGGCGGCCCCCCTTTTTC.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTa                                  <  1:4638453/96‑1 (MQ=255)
                                                                          ccGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGc                            >  2:7197490/1‑101 (MQ=255)
                                                                           cGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGcc                           >  1:2166837/1‑101 (MQ=255)
                                                                           cGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGGGcc                           >  1:1281192/1‑101 (MQ=255)
                                                                            gaggggggggCGCCCCCCCCTTTTC.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGccc                          <  1:7544629/96‑1 (MQ=255)
                                                                            gAAGATGGGTCCTCGCCCCCTTTTT.CCTGACCCAACCGTGATCGCGGCCCCCGCCTGCCGCTCGCGCCTTGCCATGGCTGGGGGGGGCGGCTAgggggtcc                          >  2:9055557/1‑96 (MQ=255)
                                                                             aaGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAggggggccc                         >  2:7039922/1‑95 (MQ=255)
                                                                              agagGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCccgc                        >  2:75701/1‑101 (MQ=255)
                                                                              agagGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCccgc                        >  1:7494328/1‑101 (MQ=255)
                                                                               gggggggCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCccgcc                       <  2:2211279/99‑1 (MQ=255)
                                                                                agGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGGGCccgccg                      >  1:8238627/1‑101 (MQ=255)
                                                                                 gggggCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGa                     >  1:1948321/1‑101 (MQ=255)
                                                                                   gggCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGaaa                   >  1:8327933/1‑101 (MQ=255)
                                                                                   gggCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGaaa                   >  2:4928789/1‑101 (MQ=255)
                                                                                       ggcgCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGAAAACCg               >  1:6578698/1‑101 (MQ=255)
                                                                                        gcccccccTTTTTCCCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGAAAACCg               <  1:3496127/98‑1 (MQ=255)
                                                                                         gccccccTTTTTCCCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGAAAACCgg              <  1:2845257/99‑1 (MQ=255)
                                                                                           cccctTTTTC.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGAAAACCGGGGc           <  1:5073670/101‑1 (MQ=255)
                                                                                            ccctTTTTC.CCTGACACACCCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGAAAACCGGGGCa          <  2:4754978/101‑1 (MQ=255)
                                                                                            ccctTTTTC.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGAAAACCGGGGCa          <  2:4634167/101‑1 (MQ=255)
                                                                                            ccccTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGAAAACCGGGGCa          >  1:152752/1‑101 (MQ=255)
                                                                                               cTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGAAAACCGGGGCAACg       >  2:4083389/1‑101 (MQ=255)
                                                                                               cTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGAAAACCGGGGCAACg       <  1:4130282/101‑1 (MQ=255)
                                                                                                 tttc.CCTGACACACCCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGAAAACCGGGGCAACGcc     <  2:8539363/101‑1 (MQ=255)
                                                                                                  ttt.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGAAAACCGGGGCAACGccc    >  1:936411/1‑101 (MQ=255)
                                                                                                   tt.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGAAAACCGGGGCAACGcccc   >  2:4011808/1‑101 (MQ=255)
                                                                                                   tt.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGAAAACCGGGGCAACGcccc   >  2:5726096/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
TCGGCGCCAGCTTCTTTGCCCGGCTGGTCGAGCGCGCGCTTCCGGCCCGCTGAGGCGGGGGGACTTCGGCGCAGCCGAAGAGGGGGCGGCGCCCCCTTTTT.CCTGACACAACCGTGATCGCGGCCCCGGCCTGCCGCTCGCGCCTTGCCATTGCGGGGGGGCGCGGCTAGGGTGCCCGCCGAAAACCGGGGCAACGCCCCA  >  NC_014034/1927109‑1927309

Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 21 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG