Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,948,873 +G 100% coding (1047/2472 nt) quiP ← acyl‑homoserine‑lactone acylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,948,8731.→G100.0% 1460.9 386coding (1047/2472 nt)quiPacyl‑homoserine‑lactone acylase
Reads supporting (aligned to +/- strand):  new base (167/219):  ref base (0/0):  total (167/219)

Only 100 of 425 total aligned reads displayed.

TTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G..CCC.A.CCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTGCGCC  >  NC_014034/1948775‑1948967
                                                                                                        |                                                                                              
ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTa                                                                                                 <  1:7065114/101‑1 (MQ=255)
gtttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTa                                                                                                 <  2:4278836/100‑1 (MQ=255)
   tCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTagcg                                                                                              <  1:8468008/101‑4 (MQ=255)
   tCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTagcg                                                                                              >  1:1399237/1‑98 (MQ=255)
    cGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTagcgc                                                                                             >  2:4114347/1‑97 (MQ=255)
     ggTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTagcgct                                                                                            <  1:518629/101‑6 (MQ=255)
     ggTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTagcgct                                                                                            >  2:1031836/1‑96 (MQ=255)
      gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTc                                                                                           <  2:8762781/101‑1 (MQ=255)
      gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GA.CCCAA.CCCTCGGGGCTGCGGTAGCGCTc                                                                                           >  1:6307553/1‑101 (MQ=255)
            cgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGt                                                                                     <  2:8412500/101‑1 (MQ=255)
            cgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGt                                                                                     >  1:2147708/1‑101 (MQ=255)
            cgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGt                                                                                     >  1:425168/1‑101 (MQ=255)
             gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                                                    <  1:5342164/101‑1 (MQ=255)
             gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                                                    <  1:3640566/101‑1 (MQ=255)
              cAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGttt                                                                                   >  1:286842/1‑101 (MQ=255)
              cAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGttt                                                                                   <  2:2528941/101‑1 (MQ=255)
               agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                                                  >  2:2372092/1‑101 (MQ=255)
               agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                                                  >  2:7993348/1‑101 (MQ=255)
               agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCC.ATCCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                                                  <  2:1780218/101‑1 (MQ=255)
                gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                                                 <  2:3786435/101‑1 (MQ=255)
                gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                                                 <  1:368741/101‑1 (MQ=255)
                gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                                                 <  2:9170988/101‑1 (MQ=255)
                  gtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCgg                                                                               >  1:1149428/1‑101 (MQ=255)
                   tgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGa                                                                              >  1:494713/1‑101 (MQ=255)
                   tgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GGCCCC.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTGTTCGGa                                                                              >  2:8217895/1‑101 (MQ=255)
                     agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                                            <  2:5145795/101‑1 (MQ=255)
                     agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGCATc                                                                            <  1:7238399/101‑1 (MQ=255)
                      gggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCa                                                                           >  1:6415785/1‑101 (MQ=255)
                       ggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCaa                                                                          >  2:4186048/1‑101 (MQ=255)
                       ggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCaa                                                                          <  2:8895135/101‑1 (MQ=255)
                         tgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGt                                                                        <  1:4452112/101‑1 (MQ=255)
                          gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                                                       <  1:6090424/101‑1 (MQ=255)
                          gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGAAGGGCTCGGGCGTTTTCGGATCAAGtt                                                                       >  2:5778218/1‑101 (MQ=255)
                             cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                                                    >  2:3931963/1‑101 (MQ=255)
                              ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                                                   <  2:3426138/101‑1 (MQ=255)
                              ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                                                   <  2:51837/101‑1 (MQ=255)
                                    gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                                             <  2:7059947/101‑1 (MQ=255)
                                     cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGACCTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGCCGTTTTCGGATCAAGCTCCTCGACGAAc                                                            <  1:8088205/101‑1 (MQ=255)
                                     cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                                            <  1:4161358/101‑1 (MQ=255)
                                     cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                                            <  2:6210112/101‑1 (MQ=255)
                                     cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                                            <  1:8359623/101‑1 (MQ=255)
                                      aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                                           <  2:5908643/101‑1 (MQ=255)
                                      aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                                           <  1:1040020/101‑1 (MQ=255)
                                      aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                                           >  1:4616663/1‑101 (MQ=255)
                                      aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                                           <  1:6788485/101‑1 (MQ=255)
                                       tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                                          <  2:2103805/101‑1 (MQ=255)
                                       tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                                          <  2:1149410/101‑1 (MQ=255)
                                       tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                                          >  1:877433/1‑101 (MQ=255)
                                        ccTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAgg                                                         <  2:3612849/101‑1 (MQ=255)
                                        ccTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAgg                                                         >  1:7496252/1‑101 (MQ=255)
                                        ccTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAgg                                                         >  1:7130707/1‑101 (MQ=255)
                                           tGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTcc                                                      <  2:7589353/101‑1 (MQ=255)
                                           tGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTcc                                                      <  2:5385069/101‑1 (MQ=255)
                                            gACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                                                     <  1:2393730/101‑1 (MQ=255)
                                            gACCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                                                     <  1:6693540/101‑1 (MQ=255)
                                             aCCGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                                                    <  1:7173099/101‑1 (MQ=255)
                                              ccGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCg                                                   >  1:2242566/1‑101 (MQ=255)
                                              ccGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCg                                                   >  2:4768370/1‑101 (MQ=255)
                                              ccGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCg                                                   <  2:8612593/101‑1 (MQ=255)
                                              ccGTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCg                                                   <  2:2299935/101‑1 (MQ=255)
                                                gTGACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                                                 <  2:1978243/101‑1 (MQ=255)
                                                  gACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                                               <  2:6444371/101‑1 (MQ=255)
                                                  gACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                                               <  1:3899691/101‑1 (MQ=255)
                                                  gACGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                                               <  1:7811648/101‑1 (MQ=255)
                                                    cGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                                             >  1:1567399/1‑101 (MQ=255)
                                                    cGATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                                             <  2:6045253/101‑1 (MQ=255)
                                                     gATCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGGAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                                            >  2:6591251/1‑101 (MQ=255)
                                                      aTCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGtagatcgg                                           >  2:6742519/1‑94 (MQ=255)
                                                       tCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGCCATCGGCg                                          >  1:2619676/1‑101 (MQ=255)
                                                       tCACCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGAAGCGCTCGGGAGTTTTCGGATCAAGTTCCTCGACGAACAGGTACTCGTCATCTGCg                                          >  1:2896536/1‑101 (MQ=255)
                                                         aCCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGaa                                        <  1:6911703/101‑1 (MQ=255)
                                                         aCCTTGCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGaa                                        <  2:143444/101‑1 (MQ=255)
                                                              gCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACATGTCCTCGTCATCGGCGAAggcgg                                   >  1:5595261/1‑101 (MQ=255)
                                                              gCGGGTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcgg                                   <  2:369227/101‑1 (MQ=255)
                                                                gggTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGgcggcg                                 <  2:6336398/101‑1 (MQ=255)
                                                                gggTGGTGAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTCTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGgcggcg                                 >  1:5408092/1‑101 (MQ=255)
                                                                  gtggtgAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGt                               <  1:8958232/101‑1 (MQ=255)
                                                                     gtgAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcggtgctgct                            <  2:1903018/101‑8 (MQ=255)
                                                                     gtgAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGAt                            <  2:3043912/101‑1 (MQ=255)
                                                                     gtgAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGAt                            <  2:1206320/101‑1 (MQ=255)
                                                                       gAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGc                          >  2:5708491/1‑101 (MQ=255)
                                                                       gAACTCGG..CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGc                          >  2:3208643/1‑101 (MQ=255)
                                                                            ctcggcccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGcccc                       <  1:3033355/92‑1 (MQ=255)
                                                                             tcggcccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCa                      <  1:8640626/93‑1 (MQ=255)
                                                                              cggcccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAg                     >  1:1994594/8‑101 (MQ=255)
                                                                                gcccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGcc                   >  1:250959/6‑101 (MQ=255)
                                                                                gcccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGcc                   >  1:9190620/6‑101 (MQ=255)
                                                                                 cccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCa                  <  1:6001365/97‑1 (MQ=255)
                                                                                     a.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCt              <  1:57426/101‑1 (MQ=255)
                                                                                          tCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTc          >  2:2895812/1‑101 (MQ=255)
                                                                                           cGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTcc         >  2:7744652/1‑100 (MQ=255)
                                                                                            ggggCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGc        >  1:3359452/1‑101 (MQ=255)
                                                                                            ggggCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGc        >  1:7661374/1‑101 (MQ=255)
                                                                                            ggggCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGc        >  1:1800685/1‑101 (MQ=255)
                                                                                            ggggCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGc        >  1:4094784/1‑101 (MQ=255)
                                                                                               gCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgc     <  1:3759443/101‑1 (MQ=255)
                                                                                                cTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgcg    >  1:3207213/1‑101 (MQ=255)
                                                                                                cTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgcg    >  2:7156524/1‑101 (MQ=255)
                                                                                                cTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgcg    <  1:3536884/101‑1 (MQ=255)
                                                                                                cTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgcg    >  2:2797874/1‑101 (MQ=255)
                                                                                                        |                                                                                              
TTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G..CCC.A.CCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTGCGCC  >  NC_014034/1948775‑1948967

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG