BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,974,326	+G	98.8%	intergenic (‑19/+36)	rpe1 ← / ← cbbM	ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,974,326	1	.→G	98.8%	5.3 ‑0.4 ‑1.2	403	intergenic (‑19/+36)	rpe1/cbbM	ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit
Reads supporting (aligned to +/- strand): new base (208/189): ref base (2/3): total (210/193)
Fisher's exact test strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.79e-02

Only 100 of 452 total aligned reads displayed.


TGGCCTGGATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..GG.GC.GG.GG.T.G.T.CCCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGG  >  NC_014034/1974226‑1974419


tGGCCTGGATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGtt                                                                                                        <  2:8512417/101‑1 (MQ=255)


  gCCTGGATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGAAGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..gg                                                                                                    <  2:5239637/101‑1 (MQ=255)


   gcTGGGTTTCGCGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTTATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..gg.g                                                                                                  <  1:8959424/100‑1 (MQ=255)


   ccTGGATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..gg.g                                                                                                  <  2:2280424/101‑1 (MQ=255)


     tGGATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..gg.ggc                                                                                                >  1:7584225/1‑99 (MQ=255)


      ggATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..gg.ggcg                                                                                               >  1:5282897/1‑98 (MQ=255)


       gATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..gg.ggcgg                                                                                              <  2:1379859/101‑5 (MQ=255)


         tttCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GC.gg.gg                                                                                           >  1:4999679/1‑101 (MQ=255)


               ggCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GC.gg.gggggtcc                                                                                     >  2:2568545/1‑95 (MQ=255)


                gCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.T.ccc                                                                                 <  2:1797831/101‑1 (MQ=255)


                  cGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.T.ccccc                                                                               <  2:4410994/101‑1 (MQ=255)


                   gAAATCGGCGAAGCCGGCAGAAAGGCTCGTGGGGGCGATCTTGATGGAAGGGACGAACGTTACTTCTGTCTCCTGAGGGGTT..GG.GG.CG.Gg.t.gcgttacctc                                                                              >  2:9019444/1‑92 (MQ=255)


                    aaaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.T.ccccccg                                                                             <  2:8773482/101‑3 (MQ=255)


                     aaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.T.ccccccgc                                                                            >  2:694902/1‑98 (MQ=255)


                     aaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.G.G.T.ccccccgc                                                                            >  1:2052220/1‑98 (MQ=255)


                        cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCcccgcccg                                                                          <  1:3149585/101‑1 (MQ=255)


                        cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCcccgcccg                                                                          <  1:2472046/101‑1 (MQ=255)


                        cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.G.G.TCCCcccgcccg                                                                          >  1:4615662/1‑101 (MQ=255)


                         ggCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.C.G.TCCCCCCGCCCgg                                                                         >  2:1683971/1‑101 (MQ=255)


                          gCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGa                                                                        >  1:6777543/1‑101 (MQ=255)


                           cGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGAt                                                                       <  2:8430509/101‑1 (MQ=255)


                           cGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGAt                                                                       >  1:3945363/1‑101 (MQ=255)


                            gAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATa                                                                      <  2:6349105/101‑1 (MQ=255)


                                 cGGCAGAAAGGATCGGGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.G.G.TCCCCCCGCCCGGATAGCAcg                                                                 >  1:7263184/1‑100 (MQ=255)


                                 cGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCAcg                                                                 <  2:7994929/101‑2 (MQ=255)


                                   gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCAcggc                                                               >  1:5881120/1‑98 (MQ=255)


                                   gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.G.G.TCCCCCCGCCCGGATAGCAcggc                                                               >  1:9193202/1‑98 (MQ=255)


                                     aGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGt                                                            >  2:3043215/1‑101 (MQ=255)


                                     aGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGt                                                            >  2:4110366/1‑101 (MQ=255)


                                     aGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.G.G.TCCCCCCGCCCGGATAGCA.CGGCGt                                                            >  2:938371/1‑101 (MQ=255)


                                      gAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGtt                                                           >  1:3807415/1‑101 (MQ=255)


                                      gAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGtt                                                           <  1:637754/101‑1 (MQ=255)


                                       aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTc                                                          <  2:6087969/101‑1 (MQ=255)


                                       aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.G.G.TCCCCCCGCCCGGATAGCA.CGGCGTTc                                                          >  1:7650222/1‑101 (MQ=255)


                                        aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCa                                                         >  1:5186438/1‑101 (MQ=255)


                                        aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCa                                                         >  2:6535757/1‑101 (MQ=255)


                                          ggATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGt                                                       <  2:7575322/101‑1 (MQ=255)


                                          ggATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCCCCTGAGGGGTTG.GG.GCGGG.GG.G.G.TCCCCCCGCCCGGACAGCA.CGGCGTTCCGt                                                       >  2:8948512/1‑101 (MQ=255)


                                            aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCCGttc                                                     >  2:3615704/1‑100 (MQ=255)


                                            aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGttc                                                     <  2:4562118/101‑2 (MQ=255)


                                            aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGttc                                                     <  1:1260786/101‑2 (MQ=255)


                                             tCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGttca                                                    <  1:5094495/101‑3 (MQ=255)


                                              cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCAc                                                   <  1:3261646/101‑1 (MQ=255)


                                               gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                  >  1:7859497/1‑101 (MQ=255)


                                               gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                  >  1:8446163/1‑101 (MQ=255)


                                                  ggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGccc                                               >  1:745096/1‑101 (MQ=255)


                                                  ggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGccc                                               >  2:76949/1‑101 (MQ=255)


                                                  ggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGccc                                               <  2:510508/101‑1 (MQ=255)


                                                   gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                              <  1:4161707/101‑1 (MQ=255)


                                                   gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.G.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                              >  2:1996628/1‑101 (MQ=255)


                                                    ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                             <  2:1571987/101‑1 (MQ=255)


                                                    ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                             >  2:3419576/1‑101 (MQ=255)


                                                     gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                            <  1:8866838/101‑1 (MQ=255)


                                                          cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                       >  2:5653886/1‑101 (MQ=255)


                                                           ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                      >  1:346075/1‑101 (MQ=255)


                                                            tGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgc                                     >  1:2909643/1‑101 (MQ=255)


                                                             gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                    >  1:4778161/1‑101 (MQ=255)


                                                             gATGGAACGGTCGAAGGTCATGTCGGTCTCCCGAGGGGTTGGGG.GG.GG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCGCAGAGCGAcgcg                                    <  1:6583352/101‑1 (MQ=255)


                                                              aTGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACgcgc                                   >  1:9126816/1‑101 (MQ=255)


                                                               tGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGcc                                  >  1:155182/1‑101 (MQ=255)


                                                                ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                 <  2:7104408/101‑1 (MQ=255)


                                                                ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                 <  2:4999519/101‑1 (MQ=255)


                                                                ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                 >  2:3486810/1‑101 (MQ=255)


                                                                 gAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAg                                <  2:5808208/101‑1 (MQ=255)


                                                                 gAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAg                                <  2:4852632/101‑1 (MQ=255)


                                                                  aaCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGc                               >  1:7131740/1‑101 (MQ=255)


                                                                    cGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGccc                             >  1:2156499/1‑101 (MQ=255)


                                                                    cGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGccc                             >  1:6116947/1‑101 (MQ=255)


                                                                      gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                           <  1:694912/101‑1 (MQ=255)


                                                                        ggAAGGTCATGTCGGTTTCCTGAGGGGTTGGGG.GG.GG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                         <  1:1028674/100‑1 (MQ=255)


                                                                        cGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                         >  2:1201832/1‑101 (MQ=255)


                                                                        cGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                         >  2:2091328/1‑101 (MQ=255)


                                                                        cGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                         <  1:2625365/101‑1 (MQ=255)


                                                                         gAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                        <  1:4912015/101‑1 (MQ=255)


                                                                         gAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                        >  2:5486291/1‑101 (MQ=255)


                                                                            ggTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.TGG.C.CCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATagag                     <  1:1914414/101‑1 (MQ=255)


                                                                            ggTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGTGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATagag                     <  2:7162893/101‑1 (MQ=255)


                                                                            ggTCATGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATagag                     >  2:8262286/1‑101 (MQ=255)


                                                                               taTGGCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                  <  1:1130839/100‑1 (MQ=255)


                                                                                 tGTCGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt                >  1:184095/1‑101 (MQ=255)


                                                                                    cGGTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCgg             >  1:3004844/1‑101 (MQ=255)


                                                                                     ggTCTCCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGc            <  2:7371124/101‑1 (MQ=255)


                                                                                       tctcCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt          <  2:1862659/101‑1 (MQ=255)


                                                                                       tctcCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt          >  1:3595901/1‑101 (MQ=255)


                                                                                        ctcCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc         >  1:4325843/1‑101 (MQ=255)


                                                                                         tcCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg        <  1:1424549/101‑1 (MQ=255)


                                                                                         tcCTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg        <  2:7641149/101‑1 (MQ=255)


                                                                                         tcCTGAGGGGTT..GGCGCGGG.GG.T.C.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGTCATCg        <  2:6472331/101‑1 (MQ=255)


                                                                                          ccTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGt       >  1:8005112/1‑101 (MQ=255)


                                                                                          ccTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGt       <  2:9126394/101‑1 (MQ=255)


                                                                                          ccTGAGGGGTTG.GG.GC.GGAGG.A.GCC.CCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGGTAGAGCGTGTCgggcacgt       >  2:2895398/1‑95 (MQ=255)


                                                                                           cTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt      >  1:4257888/1‑101 (MQ=255)


                                                                                           cTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt      <  2:3996313/101‑1 (MQ=255)


                                                                                           cTGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAATTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt      >  1:5411474/1‑101 (MQ=255)


                                                                                            tGAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc     <  1:4501273/101‑1 (MQ=255)


                                                                                             gAGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg    <  1:237724/101‑1 (MQ=255)


                                                                                              gggggTTG.GG.GC.GG.GGCT.G.TCCCCCCGCCCGGTTAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCgg   <  2:6768260/100‑1 (MQ=255)


                                                                                              aGGGGTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCgg   >  1:1361196/1‑101 (MQ=255)


                                                                                               ggggTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCggg  <  2:3244444/101‑1 (MQ=255)


                                                                                               ggggTTG.GG.GCGGG.GG.T.G.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCggg  <  1:820839/101‑1 (MQ=255)


TGGCCTGGATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..GG.GC.GG.GG.T.G.T.CCCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGG  >  NC_014034/1974226‑1974419

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG