BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,974,335	+G	1.1%	intergenic (‑28/+27)	rpe1 ← / ← cbbM	ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,974,335	1	.→G	1.1%	5.2 ‑3.5 0.0	449	intergenic (‑28/+27)	rpe1/cbbM	ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit
Reads supporting (aligned to +/- strand): new base (0/5): ref base (226/218): total (226/223)
Fisher's exact test strand distribution p-value = 2.95e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 464 total aligned reads displayed.


GGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..G.GGC..GGGG.T.GT.CCCCC.GCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGC  >  NC_014034/1974241‑1974433


  ccGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GT.cccc                                                                                               <  2:8067292/101‑1 (MQ=255)


    gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GT.cccccc                                                                                             <  2:7096467/101‑2 (MQ=255)


    gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.G.GT.cccccc                                                                                             >  1:6099059/1‑100 (MQ=255)


     aaaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GT.ccccccg                                                                                            >  2:843760/1‑99 (MQ=255)


     aaaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GT.ccccccg                                                                                            <  1:3170549/101‑3 (MQ=255)


       aTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..GCGGCG.GGGG.T.GT.ccccccgcc                                                                                          <  1:8225605/101‑5 (MQ=255)


         cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCccc.gcccg                                                                                        <  1:2472046/101‑1 (MQ=255)


          ggCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCgg                                                                                       >  1:1680528/1‑101 (MQ=255)


            ggAAGTCGGCGGAAAGGATCGAGGGGGCGATCGTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGAt                                                                                     <  1:6766584/100‑1 (MQ=255)


               aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGc                                                                                  <  1:7324257/101‑1 (MQ=255)


                   ggCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.G.GTCCCCCC.GCCCGGATAGCAcgg                                                                              >  1:701016/1‑99 (MQ=255)


                    gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCAcggc                                                                             >  2:2088685/1‑98 (MQ=255)


                    gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCAcggc                                                                             >  2:4431639/1‑98 (MQ=255)


                    gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.G.GTCCCCCC.GCCCGGATAGCAcggc                                                                             >  1:9193202/1‑98 (MQ=255)


                      aGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGt                                                                          >  1:5798004/1‑101 (MQ=255)


                      aGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGt                                                                          >  2:4110366/1‑101 (MQ=255)


                       gAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGtt                                                                         <  1:637754/101‑1 (MQ=255)


                       gAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGtt                                                                         >  2:7238546/1‑101 (MQ=255)


                        aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTc                                                                        <  2:1799215/101‑1 (MQ=255)


                        aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.G.GTCCCCCC.GCCCGGATAGCA.CGGCGTTc                                                                        >  1:7650222/1‑101 (MQ=255)


                          aGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.G.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAg                                                                      >  1:4952878/1‑101 (MQ=255)


                             aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGttc                                                                   <  1:1260786/101‑2 (MQ=255)


                             aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGttc                                                                   <  2:1696359/101‑2 (MQ=255)


                             aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGttc                                                                   <  2:4562118/101‑2 (MQ=255)


                               cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCAc                                                                 >  2:6395707/1‑101 (MQ=255)


                               cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCAc                                                                 <  1:5213029/101‑1 (MQ=255)


                                gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGttcaag                                                                >  2:944014/1‑97 (MQ=255)


                                gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACg                                                                >  1:7859497/1‑101 (MQ=255)


                                 aGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGc                                                               >  1:320213/1‑101 (MQ=255)


                                  gggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGcc                                                              >  2:3327801/1‑101 (MQ=255)


                                   ggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGccc                                                             <  2:510508/101‑1 (MQ=255)


                                    gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                                            <  2:3950719/101‑1 (MQ=255)


                                    gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                                            <  1:4161707/101‑1 (MQ=255)


                                     ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                                           <  2:2994211/101‑1 (MQ=255)


                                      gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                          >  2:913538/1‑101 (MQ=255)


                                      gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.G.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGGTCACGCCCaga                                                          >  1:8453435/1‑101 (MQ=255)


                                       cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCagag                                                         >  2:4870554/1‑101 (MQ=255)


                                        gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.G.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                                        >  1:1927573/1‑101 (MQ=255)


                                           cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                                     >  1:3472012/1‑101 (MQ=255)


                                              gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                                  >  1:4778161/1‑101 (MQ=255)


                                              gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                                  <  2:9093456/101‑1 (MQ=255)


                                              gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcaga                                                  >  2:6631499/1‑98 (MQ=255)


                                               aTGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACgcgc                                                 >  1:4580488/1‑101 (MQ=255)


                                                tGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGcc                                                <  1:7567259/101‑1 (MQ=255)


                                                    aCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGcc                                            >  1:1845777/1‑101 (MQ=255)


                                                    aCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.A.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCCGAGCGACGCGCCAGcc                                            >  2:648391/1‑101 (MQ=255)


                                                     cGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGccc                                           <  2:5616144/101‑1 (MQ=255)


                                                     cGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..G.GGCCGGGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCCGTTCACGCCCAGAGCGACGCGCCAGccc                                           <  1:6523021/101‑1 (MQ=255)


                                                         ggAAGGTCATGTCGGTTTCCTGAGGGGTTGGG.GGG..GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                                       <  1:1028674/100‑1 (MQ=255)


                                                          tccgatcttGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                                      <  1:4469107/93‑1 (MQ=255)


                                                          gAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                                      >  2:7436359/1‑101 (MQ=255)


                                                           atggTCATGTCGGTCGCCTGAGGCATTG.G.GGCG.GGGG.T.TTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCTCCGAGCGACGCGACAGCCCGGATag                                     <  2:783859/99‑1 (MQ=255)


                                                           aaGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                                     >  1:9126214/1‑101 (MQ=255)


                                                           aaGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                                     <  2:832667/101‑1 (MQ=255)


                                                           aaGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                                     <  2:1976010/101‑1 (MQ=255)


                                                             ggTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATagag                                   >  1:8873520/1‑101 (MQ=255)


                                                             ggTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GT.CCCCCAGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAAGCGCCAGCCCGGATagag                                   >  1:4235303/1‑101 (MQ=255)


                                                             ggTCATGTCGGGCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATagag                                   <  2:7653864/101‑1 (MQ=255)


                                                                taTGGCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                                <  1:1130839/100‑1 (MQ=255)


                                                                cATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.TGGC.CCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                                <  1:2220424/101‑1 (MQ=255)


                                                                cATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                                >  2:3091108/1‑101 (MQ=255)


                                                                 aTGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                               <  2:7131436/101‑1 (MQ=255)


                                                                 aTGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                               >  1:2904246/1‑101 (MQ=255)


                                                                  tGTAGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt                              >  1:7234118/1‑101 (MQ=255)


                                                                   gTCGGTGTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTc                             <  1:6905783/101‑1 (MQ=255)


                                                                   gTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTc                             <  2:346067/101‑1 (MQ=255)


                                                                   gTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTc                             <  2:5797769/101‑1 (MQ=255)


                                                                     cGGTCTCCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCgg                           >  1:3004844/1‑101 (MQ=255)


                                                                        tctcCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt                        <  2:162522/101‑1 (MQ=255)


                                                                        tctcCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt                        >  1:5151444/1‑101 (MQ=255)


                                                                        tctcCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt                        >  1:3595901/1‑101 (MQ=255)


                                                                         ctcCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc                       >  1:4325843/1‑101 (MQ=255)


                                                                         ctcCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc                       <  2:8875280/101‑1 (MQ=255)


                                                                          tcCTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg                      <  1:1424549/101‑1 (MQ=255)


                                                                            cTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAATTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt                    >  1:5411474/1‑101 (MQ=255)


                                                                            cTGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.AGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt                    >  1:5060/1‑101 (MQ=255)


                                                                             tGAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc                   >  2:1340429/1‑101 (MQ=255)


                                                                              gAGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg                  <  1:237724/101‑1 (MQ=255)


                                                                               gggggTTG.G.GGC..GGGGCT.GTCCCCCC.GCCCGGTTAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCgg                 <  2:6768260/100‑1 (MQ=255)


                                                                               aGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCgg                 >  2:4801312/1‑101 (MQ=255)


                                                                               aGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCgg                 >  1:1361196/1‑101 (MQ=255)


                                                                               aGGGGTTG.G.GGCG.GGGG.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCgg                 >  2:8575909/1‑101 (MQ=255)


                                                                                ggggTTG.G.GGCG.GGGG.T.GTCCCCCC.GCACGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCggg                <  1:3823643/101‑1 (MQ=255)


                                                                                      gttggggcggggg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAg             <  2:3699529/92‑1 (MQ=255)


                                                                                      gttggggcggggg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAg             >  2:276612/10‑101 (MQ=255)


                                                                                        tggggcggggg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGCAGCt           >  2:1115144/8‑101 (MQ=255)


                                                                                        tggggcggggg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt           >  1:2584346/8‑101 (MQ=255)


                                                                                         ggggcggggg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCtt          >  2:758751/7‑101 (MQ=255)


                                                                                          gggcggggg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCttt         <  2:5283424/96‑1 (MQ=255)


                                                                                          gggcggggg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCttt         <  1:807365/96‑1 (MQ=255)


                                                                                           ggcggggg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTc        <  1:2088747/97‑1 (MQ=255)


                                                                                           ggcggggg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTc        <  1:1564642/97‑1 (MQ=255)


                                                                                             cggggg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGa      >  2:2545961/3‑101 (MQ=255)


                                                                                             cggggg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGa      >  1:904511/3‑101 (MQ=255)


                                                                                               gggg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAAc    >  2:5423115/1‑101 (MQ=255)


                                                                                               gggg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAAc    <  2:2291658/101‑1 (MQ=255)


                                                                                                ggg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACg   <  2:6098822/101‑1 (MQ=255)


                                                                                                ggg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACg   <  2:5880885/101‑1 (MQ=255)


                                                                                                 gg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGc  >  1:3434938/1‑101 (MQ=255)


                                                                                                 gg.T.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGc  >  1:3967588/1‑101 (MQ=255)


GGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..G.GGC..GGGG.T.GT.CCCCC.GCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGC  >  NC_014034/1974241‑1974433

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG