Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,974,337 +C 98.1% intergenic (‑30/+25) rpe1 ← / ← cbbM ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,974,3371.→C98.1% 12.5 ‑1.8 ‑1.3417intergenic (‑30/+25)rpe1/cbbMribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit
Reads supporting (aligned to +/- strand):  new base (215/194):  ref base (2/6):  total (217/200)
Fisher's exact test strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.54e-02

Only 100 of 463 total aligned reads displayed.

GGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..GG.GC..GGGGT.GT.CCCCC.GCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGC  >  NC_014034/1974241‑1974433
                                                                                                       |                                                                                                 
 gCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GT.ccc                                                                                                <  2:1797831/101‑1 (MQ=255)
    gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGG.GT.cccccc                                                                                             >  1:6099059/1‑100 (MQ=255)
     aaaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTGTCCTGAGGGGTTG.GG.GCG.GGGGG.GT.ccccccg                                                                                            >  2:6614240/1‑99 (MQ=255)
     aaaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GT.ccccccg                                                                                            <  1:3170549/101‑3 (MQ=255)
         cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCccc.gcccg                                                                                        <  1:2472046/101‑1 (MQ=255)
          ggCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCgg                                                                                       >  1:1680528/1‑101 (MQ=255)
            cGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGAt                                                                                     <  2:8862454/101‑1 (MQ=255)
            cGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGAt                                                                                     <  2:8430509/101‑1 (MQ=255)
            cGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGG.GTCCCCCC.GCCCGGAt                                                                                     >  2:3460138/1‑101 (MQ=255)
             gAAGTCGGCAGAAAGGATCGAGGGGGCGATCTCGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATa                                                                                    <  2:6763241/101‑1 (MQ=255)
               aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGc                                                                                  <  1:7324257/101‑1 (MQ=255)
                 tCGGCAGAAAGGATCGAGGGGGCGATATTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCAc                                                                                >  2:1884799/1‑101 (MQ=255)
                  cGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCAcg                                                                               <  2:7994929/101‑2 (MQ=255)
                   ggCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGG.GTCCCCCC.GCCCGGATAGCAcgg                                                                              >  1:701016/1‑99 (MQ=255)
                    gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCAcggc                                                                             >  2:4431639/1‑98 (MQ=255)
                    gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCAcggc                                                                             >  2:2088685/1‑98 (MQ=255)
                      aGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGt                                                                          >  1:5798004/1‑101 (MQ=255)
                        aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTc                                                                        >  2:7076050/1‑101 (MQ=255)
                         aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCa                                                                       <  1:7187554/101‑1 (MQ=255)
                          aGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGG.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAg                                                                      >  1:4952878/1‑101 (MQ=255)
                             aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGttc                                                                   <  2:1696359/101‑2 (MQ=255)
                             aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGttc                                                                   <  1:1260786/101‑2 (MQ=255)
                             aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGttc                                                                   >  2:5850653/1‑100 (MQ=255)
                              tCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGG.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAggtca                                                                  >  2:7416775/1‑97 (MQ=255)
                               cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCAc                                                                 >  2:6395707/1‑101 (MQ=255)
                               cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCAc                                                                 <  1:5213029/101‑1 (MQ=255)
                                gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGttcaag                                                                >  2:944014/1‑97 (MQ=255)
                                gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACg                                                                <  2:2935209/101‑1 (MQ=255)
                                gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACg                                                                >  1:7641476/1‑101 (MQ=255)
                                gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACg                                                                >  1:7859497/1‑101 (MQ=255)
                                 aGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGc                                                               >  1:320213/1‑101 (MQ=255)
                                    gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                                            <  1:4161707/101‑1 (MQ=255)
                                     ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                                           >  2:2523587/1‑101 (MQ=255)
                                     ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                                           <  2:1571987/101‑1 (MQ=255)
                                      gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                          >  2:913538/1‑101 (MQ=255)
                                      gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                          <  1:8866838/101‑1 (MQ=255)
                                      gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGG.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGGTCACGCCCaga                                                          >  1:8453435/1‑101 (MQ=255)
                                       cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCagag                                                         >  1:9179152/1‑101 (MQ=255)
                                        gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                                        <  2:7128381/101‑1 (MQ=255)
                                        gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGG.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                                        >  1:1927573/1‑101 (MQ=255)
                                         aTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCg                                                       >  2:3288196/1‑101 (MQ=255)
                                           cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                                     <  2:5612566/101‑1 (MQ=255)
                                           cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                                     <  1:6738129/101‑1 (MQ=255)
                                            ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                                    >  2:5084334/1‑101 (MQ=255)
                                            ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                                    <  1:4291205/101‑1 (MQ=255)
                                              gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                                  >  2:4867171/1‑101 (MQ=255)
                                              gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                                  <  2:9093456/101‑1 (MQ=255)
                                              gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                                  >  1:4778161/1‑101 (MQ=255)
                                               aTGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACgcgc                                                 >  1:4580488/1‑101 (MQ=255)
                                               aTGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGAGTTCAGTTCACGCCCCGAGCGACgcgc                                                 >  2:5225439/1‑101 (MQ=255)
                                                tGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGcc                                                <  1:7567259/101‑1 (MQ=255)
                                                 ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                               >  1:8068285/1‑101 (MQ=255)
                                                    aCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGcc                                            >  1:1845777/1‑101 (MQ=255)
                                                     cGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..GG.GCCGGGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCCGTTCACGCCCAGAGCGACGCGCCAGccc                                           <  1:6523021/101‑1 (MQ=255)
                                                         ggAAGGTCATGTCGGTTTCCTGAGGGGTTGGGG.GG..GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                                       <  1:1028674/100‑1 (MQ=255)
                                                         cGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                                       <  2:4088852/101‑1 (MQ=255)
                                                         cGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                                       >  1:346876/1‑101 (MQ=255)
                                                         cGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                                       >  2:7828352/1‑101 (MQ=255)
                                                          tccgatcttGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                                      <  1:4469107/93‑1 (MQ=255)
                                                           aaGGTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                                     >  1:9126214/1‑101 (MQ=255)
                                                             ggTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATagag                                   >  2:8262286/1‑101 (MQ=255)
                                                             ggTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATagag                                   >  1:8225504/1‑101 (MQ=255)
                                                             ggTCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GT.CCCCCAGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAAGCGCCAGCCCGGATagag                                   >  1:4235303/1‑101 (MQ=255)
                                                               tCATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCt                                 <  1:5027300/101‑1 (MQ=255)
                                                                taTGGCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                                <  1:1130839/100‑1 (MQ=255)
                                                                cATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGTGGC.CCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                                <  1:2220424/101‑1 (MQ=255)
                                                                cATGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                                >  2:3091108/1‑101 (MQ=255)
                                                                 aTGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                               >  1:2904246/1‑101 (MQ=255)
                                                                 aTGTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                               >  2:2207621/1‑101 (MQ=255)
                                                                   gTCGGTGTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTc                             <  1:6905783/101‑1 (MQ=255)
                                                                   gTCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTc                             <  2:5797769/101‑1 (MQ=255)
                                                                    tCGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCg                            >  1:6176142/1‑101 (MQ=255)
                                                                     cGGTCTCCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCgg                           >  1:3004844/1‑101 (MQ=255)
                                                                        tctcCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt                        >  1:3595901/1‑101 (MQ=255)
                                                                        tctcCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt                        >  1:5151444/1‑101 (MQ=255)
                                                                          tcCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg                      <  1:1424549/101‑1 (MQ=255)
                                                                          tcCTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg                      <  2:7641149/101‑1 (MQ=255)
                                                                          tcCTGAGGGGTT..GGCGCG.GGGGT.CTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGTCATCg                      <  2:6472331/101‑1 (MQ=255)
                                                                            cTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt                    <  2:5381964/101‑1 (MQ=255)
                                                                            cTGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt                    >  1:5402056/1‑101 (MQ=255)
                                                                             tGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGGGCGACGCGCCAGCCCCGATAGAGCTGGCCGGCATCGTTc                   >  2:609579/1‑101 (MQ=255)
                                                                             tGAGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc                   >  2:1340429/1‑101 (MQ=255)
                                                                               aGGGGTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCgg                 >  1:1361196/1‑101 (MQ=255)
                                                                                ggggTTG.GG.GCG.GGGGT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCggg                <  2:7233814/101‑1 (MQ=255)
                                                                                ggggTTG.GG.GCG.GGGGT.GTCCCCCC.GCACGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCggg                <  1:3823643/101‑1 (MQ=255)
                                                                                      gttggggcgggggT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAg             <  2:3699529/92‑1 (MQ=255)
                                                                                      gttggggcgggggT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAg             >  2:276612/10‑101 (MQ=255)
                                                                                        tggggcgggggT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGCAGCt           >  2:1115144/8‑101 (MQ=255)
                                                                                        tggggcgggggT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt           >  1:2584346/8‑101 (MQ=255)
                                                                                        tggggcgggggT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt           >  2:3941413/8‑101 (MQ=255)
                                                                                         ggggcgggggT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCtt          >  2:758751/7‑101 (MQ=255)
                                                                                           ggcgggggT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTc        <  1:2088747/97‑1 (MQ=255)
                                                                                           ggcgggggT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTc        <  1:9010494/97‑1 (MQ=255)
                                                                                           ggcgggggT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTc        <  1:1564642/97‑1 (MQ=255)
                                                                                           ggcgggggT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTc        <  2:8572994/97‑1 (MQ=255)
                                                                                           ggcgggggT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTc        <  1:3951457/97‑1 (MQ=255)
                                                                                              gggggT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGaa     <  1:2376405/100‑1 (MQ=255)
                                                                                               ggggT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAAc    >  2:4703811/1‑101 (MQ=255)
                                                                                                 ggT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGc  >  1:3434938/1‑101 (MQ=255)
                                                                                                 ggT.GTCCCCCC.GCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGc  >  2:4532125/1‑101 (MQ=255)
                                                                                                       |                                                                                                 
GGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..GG.GC..GGGGT.GT.CCCCC.GCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGC  >  NC_014034/1974241‑1974433

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG