Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,979,921 +G 1.5% intergenic (‑37/+47) tkt1 ← / ← cbbP transketolase/phosphoribulokinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,979,9211.→G 1.5% 8.5 ‑4.3 ‑0.1404intergenic (‑37/+47)tkt1/cbbPtransketolase/phosphoribulokinase
Reads supporting (aligned to +/- strand):  new base (6/0):  ref base (191/207):  total (197/207)
Fisher's exact test strand distribution p-value = 1.29e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.51e-01

Only 100 of 470 total aligned reads displayed.

CATCGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAG  >  NC_014034/1979822‑1980021
                                                                                                    |                                                                                                      
 atcGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGcggc.gg                                                                                                      <  1:4871722/101‑1 (MQ=255)
    gCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGCTGAGCCTTTCGTCCGAGCGGC.gggtc                                                                                                   >  1:2220495/1‑99 (MQ=255)
       aggcgcacgATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.GCCgg                                                                                               >  2:6052037/9‑101 (MQ=255)
       aGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCgg                                                                                               <  1:1758463/101‑1 (MQ=255)
           cccGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGGggg                                                                                           >  1:7991776/1‑101 (MQ=255)
            ccGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTgggc                                                                                          <  1:840326/101‑1 (MQ=255)
            ccGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGGgggc                                                                                          >  2:4436306/1‑101 (MQ=255)
              ggATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.GCCGGTggggc.g                                                                                       >  1:3756770/1‑101 (MQ=255)
              ggATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTgggcc.g                                                                                       <  2:2217120/101‑1 (MQ=255)
              ggATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTgggcc.g                                                                                       <  2:3251379/101‑1 (MQ=255)
              ggATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGggggc.g                                                                                       >  2:2647218/1‑101 (MQ=255)
              cgATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTgggcc.g                                                                                       <  2:6312604/100‑1 (MQ=255)
                  ggCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGgggcc.ggggc                                                                                   >  1:4029566/1‑99 (MQ=255)
                  ggCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.ACCGCCggggc.gggcc                                                                                   >  1:5299039/1‑101 (MQ=255)
                   gCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGCGGGG.CCCGGGgggcg.gggcc                                                                                   >  2:6340928/1‑101 (MQ=255)
                    cATTGGCCATCCGCGTTTCCTGCGCCATCTCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTc                                                                                 <  1:6391940/101‑1 (MQ=255)
                    cATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTc                                                                                 <  2:1033907/101‑1 (MQ=255)
                    cATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGGgggcc.ggggccc                                                                                 >  2:3353746/1‑97 (MQ=255)
                     aTTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCg                                                                                <  2:2839485/101‑1 (MQ=255)
                      ttGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGc                                                                               <  1:5099429/101‑1 (MQ=255)
                      ttGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGc                                                                               <  1:1178657/101‑1 (MQ=255)
                       tGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGcc                                                                              <  1:5463813/101‑1 (MQ=255)
                        ggCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGGC.GGGGCTCGCCa                                                                             >  2:1658081/1‑101 (MQ=255)
                          ccATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCCGGGgggcc.gggcccgcccat                                                                            >  2:8756569/1‑94 (MQ=255)
                          ccATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAAt                                                                           <  2:4112825/101‑1 (MQ=255)
                          ccATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAAt                                                                           <  2:5642073/101‑1 (MQ=255)
                          ccATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGggggc.ggggcccccccaa                                                                           >  1:8943085/1‑91 (MQ=255)
                          ccATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCCGGGGCC.GGGGCCCGCCaca                                                                           >  2:2729156/1‑99 (MQ=255)
                            aTCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGTGGGCC.GGGCCCCGCCCATGt                                                                         >  2:3010351/1‑101 (MQ=255)
                              ccGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCt                                                                       <  1:7506210/101‑1 (MQ=255)
                                gcgTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCCCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTg                                                                     <  2:8567124/101‑1 (MQ=255)
                                 cgTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGGC.GGGGCGCGccccagtcttta                                                                    >  2:3963537/1‑91 (MQ=255)
                                  gTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGaa                                                                   <  1:7075339/101‑1 (MQ=255)
                                  gTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGAGGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGGC.GGGCCCCGCCAACATCtttta                                                                   >  2:6254756/1‑98 (MQ=255)
                                   tttCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGCGGGGC.GGGGCCCGCCCAAGTCttcaca                                                                  >  1:6703813/1‑97 (MQ=255)
                                     tCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATc                                                                <  2:5836054/101‑1 (MQ=255)
                                     tCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCC.CGGGCTCGCCCATGTCTTGTAATc                                                                >  1:301350/1‑101 (MQ=255)
                                      ccTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGGGGGCC.GGGCCTCTCCCATCTCTTGaaaatg                                                               >  2:6629372/1‑98 (MQ=255)
                                       cTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGt                                                              <  2:6948439/101‑1 (MQ=255)
                                       cTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCC.CGGGCTCCCCCATGTCTTGGAATCGt                                                              >  1:181458/1‑101 (MQ=255)
                                         gcgcCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTc                                                            <  2:5175604/101‑1 (MQ=255)
                                          ccccATATCGAGACCCTTCATGGCTCCCCCCCTCTGATGGGCTTTTCGTCCGGGGGGC.GGC.CCGGGGGGCCC.GGCCCTCGCCAATGTCTTGAAATCGTTcc                                                           <  2:6748112/99‑1 (MQ=255)
                                           gcCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCg                                                          <  1:4938220/101‑1 (MQ=255)
                                             cATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGGC.GGGCCTCGCCCATGTCTTGAAATCATTTCGGt                                                        >  2:925000/1‑101 (MQ=255)
                                             cATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGt                                                        <  2:2071436/101‑1 (MQ=255)
                                             cATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCCGGGGCCCCGCCATTTCCTTGaatcagtccggg                                                         >  1:6820787/1‑91 (MQ=255)
                                              atatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGGGGGGC.GGGCCTCGCCCATTGCTTGAAAACGTTCCCGtt                                                       >  2:8055272/1‑101 (MQ=255)
                                               tatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTc                                                      <  2:7237247/101‑1 (MQ=255)
                                                atCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCa                                                     <  2:8622715/101‑1 (MQ=255)
                                                agtgaggTCGTTCAGGGCTCCTCCTCCCTTCTGCTCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGTCCTCGCCAATGTCTTGAAATCGTTCCGGTTCa                                                     <  2:4621832/98‑1 (MQ=255)
                                                  cGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTTCGGGTCAgg                                                   >  2:7632500/1‑101 (MQ=255)
                                                   gagaTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGCGGCCC.CGGCCTCGCCGATGTCCTTAAAACATTTCGGGTcccgc                                                  >  2:2430971/1‑97 (MQ=255)
                                                    agaTCCTTCATGGCTCCTCCTCCCTGATGAGCATTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGcc                                                 <  2:5016872/101‑1 (MQ=255)
                                                     gaTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGGC.GGGCCCCGCCCAAGTCTTTGAAACGTTTCCGGTCAGGccc                                                >  1:4710416/1‑101 (MQ=255)
                                                      aTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCcg                                               <  1:767305/101‑1 (MQ=255)
                                                        ccTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGGGGGGC.GGGGCTCGCCCATGTCTTTGAATCGTTCCGGGTCCGGGCcgcc                                             >  2:549952/1‑100 (MQ=255)
                                                            cATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGGC.GGGCCCCGCCCATGTCTTGAAATCGTTTCGGTTCAGGGCcccgcgcg                                         >  2:3752250/1‑101 (MQ=255)
                                                            cATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCcgcgcgcg                                         <  2:4340550/101‑1 (MQ=255)
                                                            cATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCcgcgcgcg                                         <  2:593508/101‑1 (MQ=255)
                                                            cATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCC.GGGCCTCGCCCATGTCTTTAAAACGTTTCCGGTCAGGCCcccccgcg                                         >  1:2479853/1‑101 (MQ=255)
                                                             aTGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCC.GGGCCTCGCCCATGTCTTGAAATCGTTCCGGTTTCGGCCcgcgcgcgc                                        >  2:4000928/1‑100 (MQ=255)
                                                              tGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCG.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGAc                                       <  1:3517217/101‑1 (MQ=255)
                                                              tGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCC.GGGCCCCCCCAATGTCTTGAAATCGTTCCGGGTCAGGCCCGCGGGGGAc                                       >  2:369224/1‑101 (MQ=255)
                                                               ggCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.GCCGGGGGGGC.CGGCCCCGCCAATGTCTTGGAATCCTTCCGGTTTCGGGCcgcgccggcag                                      >  2:4761202/1‑95 (MQ=255)
                                                                gCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCCCGCCCATGTCTTGAAATCGTTCCCGGTCAAGGCCCCGCGCGACgg                                     >  2:890950/1‑101 (MQ=255)
                                                                 ctcctcctcCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.GCCCGTGGGGC.GGGCCCCGCCAATGGCTTGAAAACGCTCCCGCTCAGGCCCCCGCCCCACGGc                                    >  2:8834878/1‑101 (MQ=255)
                                                                  tccttctccCTGTTGAGCCTTTCGTCCGAGCGGC.GGG.CCCTGTGGTCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGcc                                   <  1:7361890/101‑1 (MQ=255)
                                                                    ctcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCtt                                 <  1:2765160/101‑1 (MQ=255)
                                                                    ctcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGGCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGccct                                 >  1:1509814/1‑99 (MQ=255)
                                                                     tcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTc                                <  1:1011410/101‑1 (MQ=255)
                                                                        tccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCCATGTCTTGAAATCGTTCCGGGTCCGGCCCGCGCGCGACGGGCTTcgcg                             >  2:7097234/1‑101 (MQ=255)
                                                                           cTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCC.GGGGCTCGCCAATGTCTTGAAATCGTTCCCGTTCAGGCCCGCGCGCGGCCGCCCTCGCGCAc                          >  1:3163323/1‑101 (MQ=255)
                                                                            tgatgaGCCTTTCGTCCGAGCGGC.GGG.GCCCGGGGGCC.GGGCCTCGCCCATGGCTTGAAATCCGTCCGGGTGAGGCCCCGGGGCGACGGGCCTCGCGCCcc                         >  2:4870542/1‑101 (MQ=255)
                                                                            tgatgaGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCAcc                         >  1:6558269/1‑101 (MQ=255)
                                                                             gatgaGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGGCTTGAAATCGTTCCGGTTCAGGGCCCCGCGCGCAGTCCTTCGCCCAccc                        >  2:5320747/1‑100 (MQ=255)
                                                                              atgaGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAg                       <  2:8157173/101‑1 (MQ=255)
                                                                              atgaGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAg                       <  2:6835102/101‑1 (MQ=255)
                                                                              atgaGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGGTCCGGTTCAGGCCCGCGCGCGACGGGCTTCGCGCACCAg                       >  2:8323569/1‑101 (MQ=255)
                                                                               tgaGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGGCGGCCCTCGCGCACCCGa                      >  2:1865634/1‑101 (MQ=255)
                                                                                   ccTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCt                  <  1:1958331/101‑1 (MQ=255)
                                                                                   ccTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCt                  <  1:2570853/101‑1 (MQ=255)
                                                                                    cTTGCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTc                 <  1:3373837/101‑1 (MQ=255)
                                                                                     tttCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCCGACGCTcc                >  1:6093455/1‑100 (MQ=255)
                                                                                       tCGTCCGAGCGGC.GGG.CCCGGTGGTCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGAt              <  2:1110459/101‑1 (MQ=255)
                                                                                       tCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGAt              <  2:1304801/101‑1 (MQ=255)
                                                                                          tCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAg           <  1:4358556/101‑1 (MQ=255)
                                                                                           ccGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAgc          <  1:4593651/101‑1 (MQ=255)
                                                                                            cGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGGTCCgcg         >  2:9071447/1‑101 (MQ=255)
                                                                                            cGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAgcg         <  1:4096117/101‑1 (MQ=255)
                                                                                            cGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAgcg         <  2:1536242/101‑1 (MQ=255)
                                                                                             gAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAgcgg        >  1:8629870/1‑101 (MQ=255)
                                                                                             gAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAgcgg        >  1:5838251/1‑101 (MQ=255)
                                                                                                 ggc.ggG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTc    <  2:3475853/101‑1 (MQ=255)
                                                                                                 ggc.ggG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTc    >  2:4552294/1‑101 (MQ=255)
                                                                                                  gc.ggG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCa   <  1:88437/101‑1 (MQ=255)
                                                                                                   c.ggG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGGGGCGTCAg  >  1:918945/1‑101 (MQ=255)
                                                                                                   c.ggG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAg  >  1:7268036/1‑101 (MQ=255)
                                                                                                   c.ggG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAg  >  2:7910330/1‑101 (MQ=255)
                                                                                                   c.ggG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAg  >  1:5673516/1‑101 (MQ=255)
                                                                                                   c.ggG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTAAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAg  >  1:8212032/1‑101 (MQ=255)
                                                                                                    |                                                                                                      
CATCGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCC.GGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAG  >  NC_014034/1979822‑1980021

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG