BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,997,981	+G	100%	intergenic (‑46/‑119)	RCAP_rcc01844 ← / → RCAP_rcc01845	sensor histidine kinase/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,997,981	1	.→G	100.0%	52.2	478	intergenic (‑46/‑119)	RCAP_rcc01844/RCAP_rcc01845	sensor histidine kinase/hypothetical protein
Reads supporting (aligned to +/- strand): new base (224/233): ref base (13/8): total (237/241)
Fisher's exact test strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.59e-04

Only 100 of 516 total aligned reads displayed.


GCAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.G.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATGGGTTT  >  NC_014034/1997881‑1998076


 gggAATCGGAAACGGGTAGGGCCCGGCCAAGGAGATTGTTGACGCATCCGCCATTTTTCGGTCGCCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.g                                                                                                 <  2:6805425/99‑1 (MQ=255)


  aGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.g.g                                                                                               <  1:6537123/101‑1 (MQ=255)


    aaTCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGANCCGAGCAAGCTTC.g.ggg                                                                                             >  2:799497/1‑100 (MQ=255)


    aaTCCGACACGGTAGCGTCGGGGCCAAGGAGATTGTTGACGAATCCGCCAGTTTTCGGTCGCCCTGCCATACCCAAAGCTTGACCCGACCAAGCTTC.g.ggg                                                                                             <  2:6070159/101‑2 (MQ=255)


     aTCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.g.ggga                                                                                            >  2:2694483/1‑99 (MQ=255)


     aTCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.g.ggga                                                                                            >  2:8723199/1‑99 (MQ=255)


      tCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.G.GGgaa                                                                                           <  1:2796712/101‑1 (MQ=255)


         gAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACg                                                                                         >  1:2301668/1‑101 (MQ=255)


         gAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACg                                                                                         >  1:2043243/1‑101 (MQ=255)


         gAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACg                                                                                         >  1:1041422/1‑101 (MQ=255)


             cGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATAc                                                                                     <  1:5056891/101‑1 (MQ=255)


             cGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATAc                                                                                     >  1:4044858/1‑101 (MQ=255)


                tAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCa                                                                                  >  1:6729170/1‑101 (MQ=255)


                 aaCGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCaa                                                                                 <  2:7162604/101‑1 (MQ=255)


                 aaCGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCaa                                                                                 >  1:9148176/1‑101 (MQ=255)


                  aCGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAg                                                                                <  2:3963360/101‑1 (MQ=255)


                   cGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCGTTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGt                                                                               >  1:539993/1‑101 (MQ=255)


                   cGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGt                                                                               >  2:4203907/1‑101 (MQ=255)


                    gTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGtt                                                                              >  1:2990100/1‑101 (MQ=255)


                    gTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGtt                                                                              >  1:5571845/1‑101 (MQ=255)


                     tctcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTc                                                                             <  2:2499076/101‑1 (MQ=255)


                      ctcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCa                                                                            <  1:5659695/101‑1 (MQ=255)


                        cGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAg                                                                          >  2:8216183/1‑101 (MQ=255)


                        cGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAg                                                                          <  1:5849263/101‑1 (MQ=255)


                         ggCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAgg                                                                         >  2:4619732/1‑101 (MQ=255)


                          gCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTTACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGc                                                                        <  1:8763119/101‑1 (MQ=255)


                           ccAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGcc                                                                       <  2:5614725/101‑1 (MQ=255)


                            cAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCg                                                                      >  1:9038346/1‑101 (MQ=255)


                            cAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCg                                                                      >  2:6327591/1‑101 (MQ=255)


                             aaGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGc                                                                     >  1:8576307/1‑101 (MQ=255)


                                 agaTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGc                                                                 <  2:5107064/101‑1 (MQ=255)


                                  gaTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCa                                                                >  1:4696512/1‑101 (MQ=255)


                                  gaTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCa                                                                <  2:2290447/101‑1 (MQ=255)


                                   aTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCaa                                                               <  1:4369450/101‑1 (MQ=255)


                                   aTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCaa                                                               <  2:4416927/101‑1 (MQ=255)


                                   aTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCaa                                                               <  1:7377764/101‑1 (MQ=255)


                                    ttgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCaaa                                                              <  2:6614706/101‑1 (MQ=255)


                                     tgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCaaaa                                                             >  2:3840801/1‑101 (MQ=255)


                                     gtttgctaattCCGCGATTTTTGGGTCAGCGTTCCAGCTCCCGTGCTTGACCCGAGCAAGCTTCGG.GGAAACGATTCCCAAGTTCAAGGCCGCATGCaaaa                                                             <  1:8233796/91‑1 (MQ=255)


                                      gttgACGAATCCGCCATTTTTCTGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAg                                                            >  1:7725501/1‑101 (MQ=255)


                                      gttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGCCCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAACGCCGCATGCAAAAg                                                            >  2:7290802/1‑101 (MQ=255)


                                      gttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAg                                                            >  1:983657/1‑101 (MQ=255)


                                      gttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAg                                                            <  1:7292062/101‑1 (MQ=255)


                                      gttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAg                                                            >  2:3034568/1‑101 (MQ=255)


                                       ttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGc                                                           <  2:2772805/101‑1 (MQ=255)


                                        tgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGcc                                                          >  1:3755505/1‑101 (MQ=255)


                                        tgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGcc                                                          <  1:4943242/101‑1 (MQ=255)


                                          aCGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAg                                                        >  2:1204497/1‑101 (MQ=255)


                                           cGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAgg                                                       <  1:6662011/101‑1 (MQ=255)


                                            gcaTCCGCCATTTTTCGGTTACCCTGACATAGCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAggg                                                      <  1:8943124/99‑1 (MQ=255)


                                                  gCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTa                                                >  2:370555/1‑101 (MQ=255)


                                                   ccATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTaa                                               <  2:3374460/101‑1 (MQ=255)


                                                    cATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAg                                              >  1:6099054/1‑101 (MQ=255)


                                                     aTTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGc                                             >  1:8107131/1‑101 (MQ=255)


                                                      tttttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCt                                            >  2:5338018/1‑101 (MQ=255)


                                                       ttttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtt                                           >  2:4473538/1‑101 (MQ=255)


                                                       ttttCGGGCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtt                                           <  1:336592/101‑1 (MQ=255)


                                                         ttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtttt                                         >  2:5843253/1‑101 (MQ=255)


                                                         ttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtttt                                         >  2:1301566/1‑101 (MQ=255)


                                                         ttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtttt                                         <  1:4619867/101‑1 (MQ=255)


                                                         ttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtttt                                         <  2:7849254/101‑1 (MQ=255)


                                                          tCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTg                                        <  1:7535336/101‑1 (MQ=255)


                                                            ggTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCt                                      >  2:5206069/1‑101 (MQ=255)


                                                            ggTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCt                                      >  2:7502304/1‑101 (MQ=255)


                                                            ggTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCt                                      >  2:8570712/1‑101 (MQ=255)


                                                              tCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGa                                    >  1:4474543/1‑101 (MQ=255)


                                                               cACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGaa                                   >  2:7699870/1‑101 (MQ=255)


                                                               cACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGaa                                   >  1:8370334/1‑101 (MQ=255)


                                                               cACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGaa                                   <  2:1478088/101‑1 (MQ=255)


                                                                  ccTGACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTc                                <  2:831300/101‑1 (MQ=255)


                                                                     gACATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTcgcg                             <  2:4792763/101‑1 (MQ=255)


                                                                      aCATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCTCgg                            <  2:3660057/101‑1 (MQ=255)


                                                                      aCATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCgg                            <  1:1667131/101‑1 (MQ=255)


                                                                      aCATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCgg                            <  1:1260657/101‑1 (MQ=255)


                                                                       cATATCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGa                           >  2:5028763/1‑101 (MQ=255)


                                                                        atatCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAt                          >  1:3051829/1‑101 (MQ=255)


                                                                        atatCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAt                          >  2:6965023/1‑101 (MQ=255)


                                                                         tatCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtg                         <  1:4165427/101‑1 (MQ=255)


                                                                          ttCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgt                        >  2:1753800/2‑101 (MQ=255)


                                                                          atCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgt                        >  1:25912/1‑101 (MQ=255)


                                                                           tCCAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgtt                       >  2:7054000/1‑101 (MQ=255)


                                                                             cAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgt                     >  1:7942294/1‑101 (MQ=255)


                                                                             cAATGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgt                     >  1:6264771/1‑101 (MQ=255)


                                                                              aaTGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgtt                    >  1:8464095/1‑101 (MQ=255)


                                                                               aTGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgttg                   >  1:3798731/1‑101 (MQ=255)


                                                                               aTGCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgttg                   <  1:3869474/101‑1 (MQ=255)


                                                                                 gCTTGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTc                 >  1:5307008/1‑101 (MQ=255)


                                                                                    tGACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATg              <  1:2201274/101‑1 (MQ=255)


                                                                                     gACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgagatcggaa             >  1:1205004/1‑92 (MQ=255)


                                                                                     gACCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGa             <  1:1404719/101‑1 (MQ=255)


                                                                                      cgccGAGCAACCTTC.GAGGAAACGTAGGCCAAGTTCAAGGCTGCATGCAAAAGCCCGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCAtctg            <  2:9118811/99‑4 (MQ=255)


                                                                                      aCCCGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAg            >  2:1667368/1‑101 (MQ=255)


                                                                                       cccGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGc           <  1:6896747/101‑1 (MQ=255)


                                                                                         cGAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGa         >  2:3213077/1‑101 (MQ=255)


                                                                                          gAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGAt        >  1:1551025/1‑101 (MQ=255)


                                                                                          gAGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGAt        <  1:3615591/101‑1 (MQ=255)


                                                                                           aGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATg       <  1:7642178/101‑1 (MQ=255)


                                                                                           aGCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATg       >  1:1817592/1‑101 (MQ=255)


                                                                                            gCAAGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATgg      <  2:8891994/101‑1 (MQ=255)


                                                                                              aaGCTTCGG.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATGGGt    >  2:2080587/1‑101 (MQ=255)


GCAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.G.GGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATGGGTTT  >  NC_014034/1997881‑1998076

Base quality scores:


ATCG

< 3 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG