Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,098,585 +G 98.1% coding (52/1491 nt) RCAP_rcc01939 → polysaccharide biosynthesis protein family

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,098,5851.→G98.1% 4.4 ‑1.7 ‑2.0265coding (52/1491 nt)RCAP_rcc01939polysaccharide biosynthesis protein family
Reads supporting (aligned to +/- strand):  new base (115/145):  ref base (4/1):  total (119/146)
Fisher's exact test strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.01e-02

Only 100 of 384 total aligned reads displayed.

TTCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCC.G.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGTCATCACCCT  >  NC_014034/2098485‑2098682
                                                                                                        |                                                                                                   
ttCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCCGCGGG.CGT.cccc                                                                                                      >  1:4101399/1‑101 (MQ=255)
ttCGAAGGCGGTCTGGCGCCTGCACGGCACCCGCACGTTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.cccc                                                                                                      <  1:6689351/101‑1 (MQ=255)
ttCGAAGGCGGTCGGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.cccc                                                                                                      <  1:2103471/101‑1 (MQ=255)
 tCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGG.CCCC.g                                                                                                    >  1:3632553/1‑101 (MQ=255)
  cGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGG.CCCC.gg                                                                                                   >  1:1590953/1‑100 (MQ=255)
   gAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCC.ggc                                                                                                  >  2:1654691/1‑99 (MQ=255)
   gAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCC.ggc                                                                                                  >  1:1803613/1‑99 (MQ=255)
   gAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCC.ggc                                                                                                  >  2:3980609/1‑99 (MQ=255)
    aaGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGTGCCCC.G.cc                                                                                                 >  2:8813179/1‑100 (MQ=255)
    aaGGCGCTCTGGCGCCTTCACGGCCCCCGCACAGTATAGGGGACAGTGGCAGCCCTTGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCC.ggct                                                                                                 <  2:2827385/101‑4 (MQ=255)
      ggCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCC.ggctcc                                                                                               <  2:4601532/101‑6 (MQ=255)
       gCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.TCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCg                                                                                              <  1:6464475/101‑1 (MQ=255)
       gCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.CGC.CCCC.ggccccg                                                                                              >  1:4482617/1‑95 (MQ=255)
         ggTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.cc                                                                                           >  2:8196235/1‑101 (MQ=255)
          gTCTGGCCCCTTCACGGCCCCCGTATGGAACAGGGGACAGTGGCAGCCTATGCCCAGCTTTTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.ccc                                                                                          <  1:6779257/101‑1 (MQ=255)
            cTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCgg                                                                                        <  1:8177235/101‑1 (MQ=255)
             tGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGTCCCCC.G.CTCCG.CCCggg                                                                                       >  1:4911761/1‑101 (MQ=255)
               gcgcCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGcc                                                                                     <  1:7989087/101‑1 (MQ=255)
                  ccTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCACGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.CGT.CCCCGG.CGCCG.CCCGGGCCCGc                                                                                  >  1:3227526/1‑101 (MQ=255)
                   cTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGcc                                                                                 <  2:6990332/101‑1 (MQ=255)
                    ttCACGGCACCCGCCCAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCcg                                                                                <  2:2418796/101‑1 (MQ=255)
                     tCACGGCCCCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCcgc                                                                               <  2:7728883/101‑1 (MQ=255)
                      cACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGG.CCCCGG.CTCCC.CCCGGGCCAGCcgcg                                                                              >  2:1235654/1‑101 (MQ=255)
                      cACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCCCCGCGGCGGC.GGG.CCCCGG.CTCCG.CCCGGGCCCGCcgcg                                                                              >  1:3093469/1‑101 (MQ=255)
                        cGGCNCCCGCACAGTATANNGNACAGTGGCAGCCCATNNNCAGCGATTTG.CCCCCCCCCGCCGCGNNNGC.GGT.CCCCGG.CTCCG.CCCGNGCCCGCCGCggc                                                                            >  2:8073288/1‑100 (MQ=255)
                         ggCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGAc                                                                           >  1:2411477/1‑101 (MQ=255)
                          gCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGG.CCCCGG.CTCCG.CCCCGGCCAGCCGCGGAcg                                                                          >  1:1180159/1‑101 (MQ=255)
                               cGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGAcggcgcg                                                                     <  1:6360970/101‑1 (MQ=255)
                               cGCAAAGTAGGGGGGACGGGGGCCGCCCATGCCCAGCTTTTCC.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGAcggcgcg                                                                     <  2:9106836/101‑1 (MQ=255)
                                 cacaGTATAGGGTACAGTTTCGCCCCATGATCGGCGATTTG.CCCCCCCCCACCGCGGCGTC.GGA.CCCCGG.ATCCG.CCCGGGCCAGCCGCGGAcggcgcggc                                                                   <  1:9142161/101‑1 (MQ=255)
                                 cacaGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCCGGccgcgcggc                                                                   >  1:6036515/1‑101 (MQ=255)
                                   caGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG..CCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGAcggcgcggcgcg                                                                <  1:911817/101‑1 (MQ=255)
                                      tataGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGAcggcgcggcgcggc                                                              <  1:551595/101‑1 (MQ=255)
                                      tagaGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGAcggcgcggcgcggc                                                              <  1:2040824/98‑1 (MQ=255)
                                       ataGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGAcggcgcggcgcggcg                                                             >  2:6503822/1‑101 (MQ=255)
                                         aGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCCCCGCGGCGGC.GGG.CCCCGG.CTCCG.CCCGGGCCCGCCGCGGGcggcgcggcgcggccgg                                                           >  2:4394267/1‑98 (MQ=255)
                                          ggggACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCCGCCGCGGACGGCGCGGCgcggcggcg                                                          >  1:7675719/1‑101 (MQ=255)
                                            ggACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCCCCGCGGCGGC.GGG.CCCCGG.CTCCG.CCCGGGCCCGCCGCGGACGGCGCCGCgcggcgggggt                                                        >  2:2246408/1‑97 (MQ=255)
                                              aCAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGAGCt                                                      >  1:3432921/1‑101 (MQ=255)
                                               gagggccAGCCCATGAGCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCtt                                                     <  2:5152481/95‑1 (MQ=255)
                                               cAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCgcggcggggcgctt                                                     >  2:3321034/1‑94 (MQ=255)
                                               cAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCCCCGCGGCGGCGGGC.CCCC.G.CCCCG.CCCGGGCCCGCCCCCGGCGGGCCCGGgcggcggggggctt                                                     >  2:1905815/1‑94 (MQ=255)
                                                ggtgcctgCCCTTGTCCGGCGTTTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTc                                                    <  2:1599475/94‑1 (MQ=255)
                                                cttGGCGGCCCATGCCCAGCGATTTC.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTc                                                    <  1:1427129/99‑1 (MQ=255)
                                                aGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTc                                                    <  1:4306173/101‑1 (MQ=255)
                                                  tGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCCGGCGGCGCGGCGCGGCGGCGCTCTTcag                                                  >  2:1427097/1‑99 (MQ=255)
                                                     gcgCCCCTGCCCCGGGATTTGCCCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCt                                                <  2:5432819/99‑1 (MQ=255)
                                                      ggCCCATGCCCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCttt                                              <  2:8267043/100‑1 (MQ=255)
                                                        cccATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGGGGTCTTCGCCTTTgc                                            >  1:8581369/1‑100 (MQ=255)
                                                         ccATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTc                                           >  1:263006/1‑101 (MQ=255)
                                                          cATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGTCCCCC.G.CTCCG.CCCGGGCCAGCCGCGGGCGGCGCGGCGCCGCGGCGAGCTTCCGCTTTGTcg                                          >  2:4121472/1‑101 (MQ=255)
                                                          cATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGGTCTTCGCCCTTGTcg                                          >  2:4922247/1‑101 (MQ=255)
                                                            tgcccggcaaTTTG.CCCCCCCCCGCCGCGGNGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTcgcg                                        <  1:8813584/92‑1 (MQ=255)
                                                            cgACCTGCGATTTG.CCCCCCCCCCCCGCGGCGGC.GGG.CCCG.G.CCGCGCCCCGGGCCGCCCGCCGCGCCCGCGCGGCGGGGGCGGGCTTCGCCTTTGTcgcg                                        >  2:4521894/2‑101 (MQ=255)
                                                              aCCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGGGCGGCGGCGGTCTTCGGCTTTGTtgcgcg                                      >  2:7117556/1‑101 (MQ=255)
                                                                ccgggATTTC.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCggg                                    <  1:1063670/97‑1 (MQ=255)
                                                                 aGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGc                                   >  1:5401834/1‑101 (MQ=255)
                                                                  gCGATTGC.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCg                                  <  2:6336585/101‑1 (MQ=255)
                                                                  gCGATTCG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCg                                  <  2:3375668/101‑1 (MQ=255)
                                                                   gcgtttcccccccccccGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCg                                  <  2:4482482/93‑1 (MQ=255)
                                                                    gATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGAc                                >  2:5982843/1‑101 (MQ=255)
                                                                     ttttG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACg                               <  1:3469228/100‑1 (MQ=255)
                                                                     aTTCG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACg                               <  2:5707362/101‑1 (MQ=255)
                                                                      tttgccccccccccGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACg                               <  2:6703780/96‑1 (MQ=255)
                                                                        tG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGGTCTTCGCCTTTGTCGCGCCGGCGACgcag                            >  2:3787997/1‑101 (MQ=255)
                                                                        tG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACgcag                            <  2:319573/101‑1 (MQ=255)
                                                                          ccccccccccGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACgcagc                           <  1:387700/100‑1 (MQ=255)
                                                                            ccccccccGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGcagcag                         >  1:7477924/1‑101 (MQ=255)
                                                                            ccccccccGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGcagcag                         <  2:1180141/101‑1 (MQ=255)
                                                                             cccccccGCCGCGGCGGC.GGT.CCCCGG.CTCCG.CCCGTGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCagcagc                        <  1:6521584/101‑1 (MQ=255)
                                                                               cccccGCCGCGGCGGC.GGC.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCtt                      <  2:4732788/101‑1 (MQ=255)
                                                                                 cccGCCGCGGCGGC.GGC.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTc                    <  1:1294353/101‑1 (MQ=255)
                                                                                  ccgccgCGTCGGC.GGC.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCg                   <  2:7277981/101‑1 (MQ=255)
                                                                                  ccgccgCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCg                   <  1:5608843/101‑1 (MQ=255)
                                                                                  ccgccgCGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCg                   <  2:2009079/101‑1 (MQ=255)
                                                                                   cgccgcGGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCgc                  >  2:4287329/1‑100 (MQ=255)
                                                                                      cgcgGCGGC.GGT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCt               >  1:8268739/1‑101 (MQ=255)
                                                                                        gggcgcc.ggC.CCCC.GCCCCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCttt             <  2:2936270/100‑1 (MQ=255)
                                                                                         ggcggc.ggT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTg            >  1:1683863/1‑101 (MQ=255)
                                                                                         ggcggc.ggT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTg            <  1:3963426/101‑1 (MQ=255)
                                                                                           gcgccgcgccccg.G.CCCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGt           <  1:2925074/92‑1 (MQ=255)
                                                                                           cggc.ggT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtc          <  2:2341092/101‑1 (MQ=255)
                                                                                              c.ggT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtcatc       <  1:2326122/101‑1 (MQ=255)
                                                                                              c.ggT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtcatc       >  1:3786807/1‑101 (MQ=255)
                                                                                              c.ggT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtcatc       >  2:5326088/1‑101 (MQ=255)
                                                                                              c.ggT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtcatc       >  1:5929088/1‑101 (MQ=255)
                                                                                                ggT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtcatca      >  2:662641/1‑101 (MQ=255)
                                                                                                ggT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtcatca      <  1:6949918/101‑1 (MQ=255)
                                                                                                ggT.CCCCGG.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtcatca      <  2:3562104/101‑1 (MQ=255)
                                                                                                 gtccccg.G.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGTCATCAc     <  2:8035801/98‑1 (MQ=255)
                                                                                                 gtccccg.G.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCACCTTTCGACCTTTGTCATCAc     >  1:7242184/4‑101 (MQ=255)
                                                                                                 gtccccg.G.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGAGCGACGCAGCAGCTTTCGACCTTTGTCATCAc     >  1:4676062/4‑101 (MQ=255)
                                                                                                  tccccg.G.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGTCATCAcc    >  1:352256/3‑101 (MQ=255)
                                                                                                  tccccg.G.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGTCATCAcc    <  1:1726458/99‑1 (MQ=255)
                                                                                                  cccccg.G.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGTCATCAcc    <  1:4802451/99‑1 (MQ=255)
                                                                                                    cccg.G.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGTCATCACCct  <  2:867976/101‑1 (MQ=255)
                                                                                                    cccg.G.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGTCATCACCct  <  2:7477600/101‑1 (MQ=255)
                                                                                                    cccg.G.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGTCATCACCct  <  1:986693/101‑1 (MQ=255)
                                                                                                    cccg.G.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGTCATCACCct  <  1:3325336/101‑1 (MQ=255)
                                                                                                    cccg.G.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGTCATCACCct  <  1:7848275/101‑1 (MQ=255)
                                                                                                        |                                                                                                   
TTCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTG.CCCCCCCCCGCCGCGGCGGC.GGT.CCCC.G.CTCCG.CCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGTCATCACCCT  >  NC_014034/2098485‑2098682

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG