Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,135,137 +G 99.3% intergenic (‑131/+80) RCAP_rcc01974 ← / ← RCAP_rcc01975 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,135,1371.→G99.3% 2.1 0.0 ‑1.0532intergenic (‑131/+80)RCAP_rcc01974/RCAP_rcc01975hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (276/252):  ref base (2/2):  total (278/254)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.04e-01

Only 100 of 591 total aligned reads displayed.

AATGTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.GG.G.CCAAAGTTCAAAGG.C.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCC  >  NC_014034/2135037‑2135233
                                                                                                     |                                                                                                    
aaTGTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGa                                                                                                       >  1:3536637/1‑101 (MQ=255)
 aTGTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.g                                                                                                     >  2:3287295/1‑101 (MQ=255)
  tGTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.gg                                                                                                    <  2:7731346/101‑1 (MQ=255)
   gTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.gg.g                                                                                                  >  1:1998490/1‑101 (MQ=255)
   gTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.gat                                                                                                   >  1:2424721/1‑99 (MQ=255)
     tGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.gg.ggc                                                                                                >  1:4107368/1‑99 (MQ=255)
      gcaaTACGCCCATCTTTTGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.gg.ggcc                                                                                               <  2:1690073/98‑4 (MQ=255)
      gCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.gg.ggcc                                                                                               >  2:5297149/1‑98 (MQ=255)
      gCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.gg.ggcc                                                                                               >  1:5314675/1‑98 (MQ=255)
       cGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.gg.ggcca                                                                                              >  1:7443622/1‑97 (MQ=255)
        gATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.gg.ggccaa                                                                                             >  1:6031419/1‑96 (MQ=255)
        gATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.gg.ggccaa                                                                                             >  2:4620857/1‑96 (MQ=255)
         aTAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCaaa                                                                                            >  2:380747/1‑101 (MQ=255)
         aTAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCaaa                                                                                            >  2:6819118/1‑101 (MQ=255)
         aTAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCaaa                                                                                            >  1:1226858/1‑101 (MQ=255)
          tAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACCCGCGGGCTTTGAGGG.G.CCaaat                                                                                           >  2:4156466/1‑100 (MQ=255)
           aaGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGt                                                                                          >  1:3142857/1‑101 (MQ=255)
           aaGCGCAACTTAGGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGt                                                                                          <  1:760411/101‑1 (MQ=255)
           aaGCGCAACTTAGGGTAAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGt                                                                                          <  1:7113349/101‑1 (MQ=255)
            aGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGtt                                                                                         >  2:4332423/1‑101 (MQ=255)
            aGCACAACTTAGGCTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGtt                                                                                         <  1:1762948/101‑1 (MQ=255)
              cgcAATTTATGTTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCa                                                                                       <  2:7984589/101‑1 (MQ=255)
              cgcAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCa                                                                                       >  1:2259293/1‑101 (MQ=255)
               gcAATTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCaa                                                                                      <  1:6185036/101‑1 (MQ=255)
               gcAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCaa                                                                                      >  2:452613/1‑101 (MQ=255)
               gcAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCaa                                                                                      >  2:5521654/1‑101 (MQ=255)
               gcAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCaa                                                                                      >  1:8052638/1‑101 (MQ=255)
                cAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCaaa                                                                                     >  2:4761710/1‑101 (MQ=255)
                 aaCTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAg                                                                                    >  1:3796595/1‑101 (MQ=255)
                  aCTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAgg                                                                                   >  1:4403286/1‑101 (MQ=255)
                    ttATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGG.c.c                                                                               >  2:5915154/1‑101 (MQ=255)
                     tATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGG.c.cc                                                                              >  2:3617580/1‑101 (MQ=255)
                       gggcaaaGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGG.c.cccg                                                                            <  2:2592464/96‑3 (MQ=255)
                          taaaGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGG.c.cccgctt                                                                         <  1:8904949/99‑6 (MQ=255)
                           cAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCGAAGTTCAAAGGCC.CCGCttt                                                                         >  1:7227421/1‑101 (MQ=255)
                           cAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCttt                                                                         <  2:2818906/101‑1 (MQ=255)
                            aaGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGAGGGGTTTGAGGG.G.GCAAAGGTCAAAGG.c.cccagcttc                                                                       >  2:4234163/1‑94 (MQ=255)
                             aGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTcc                                                                       <  2:9152528/101‑1 (MQ=255)
                              gCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCa                                                                      <  1:2692274/101‑1 (MQ=255)
                              gCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCa                                                                      <  1:5810640/101‑1 (MQ=255)
                               ccATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAg                                                                     <  2:2550221/101‑1 (MQ=255)
                                 aTTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCg                                                                   >  1:6416947/1‑101 (MQ=255)
                                  ttAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCgg                                                                  >  1:6535618/1‑101 (MQ=255)
                                    aaaTTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCgggg                                                                >  1:7688111/1‑101 (MQ=255)
                                    aaaTTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCgggg                                                                >  2:8401600/1‑101 (MQ=255)
                                      aTTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGcc                                                              >  2:862746/1‑101 (MQ=255)
                                      aTTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGcc                                                              <  1:393398/101‑1 (MQ=255)
                                          cGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAgg                                                          <  2:2357525/101‑1 (MQ=255)
                                             ttATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCCAAGTGCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGc                                                       >  1:2966739/1‑101 (MQ=255)
                                             ttATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGc                                                       >  2:4919113/1‑101 (MQ=255)
                                              tATCGCAACTTTGCTTCCGCGTTGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGcg                                                      <  2:8783324/101‑1 (MQ=255)
                                              tATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGcg                                                      >  2:1618333/1‑101 (MQ=255)
                                              tATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGcg                                                      >  2:6995510/1‑101 (MQ=255)
                                               aTCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGcga                                                     >  2:3102667/1‑101 (MQ=255)
                                                tCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGcgac                                                    <  1:1060444/101‑1 (MQ=255)
                                                   cAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCgacgac                                                 >  1:4718649/1‑101 (MQ=255)
                                                    acatttGCGTCCCCGTCCATTCCTGGGAGCGTCGACGCGCGGGCGTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCGGCGGGGCCAAGGGGCGacgacg                                                <  2:72021/98‑1 (MQ=255)
                                                    aaCTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGacgacg                                                >  2:1437345/1‑101 (MQ=255)
                                                     aCTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACgg                                               <  1:9005508/101‑1 (MQ=255)
                                                       tttGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTg                                             >  2:765388/1‑101 (MQ=255)
                                                        ttGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGa                                            >  2:5091486/1‑101 (MQ=255)
                                                         tGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGaa                                           >  2:5687348/1‑101 (MQ=255)
                                                         tGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGaa                                           >  2:1141631/1‑101 (MQ=255)
                                                         tGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGaa                                           <  1:3028519/101‑1 (MQ=255)
                                                          gCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAg                                          >  1:1523303/1‑101 (MQ=255)
                                                          gCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAg                                          >  2:1941746/1‑101 (MQ=255)
                                                            ttCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGc                                        >  1:8728420/1‑101 (MQ=255)
                                                            ttCCACGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.GGCG.CCAAAGTTCAAACG.CAGCGCTTTCCAACGGGGCAAAATGGCGACGACTATGTAGGc                                        >  2:1236406/1‑101 (MQ=255)
                                                             tCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCa                                       >  1:8294553/1‑101 (MQ=255)
                                                              ccGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCaa                                      <  2:9000945/101‑1 (MQ=255)
                                                               cgcgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCaaa                                     <  2:8088207/101‑1 (MQ=255)
                                                                 cgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGc                                   >  1:7934217/1‑101 (MQ=255)
                                                                  gTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCa                                  <  1:6819389/101‑1 (MQ=255)
                                                                   tCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCaa                                 >  1:4973824/1‑101 (MQ=255)
                                                                    cGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAAc                                <  2:2146403/101‑1 (MQ=255)
                                                                    cGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAAc                                <  1:8153912/101‑1 (MQ=255)
                                                                    cGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAAc                                >  1:5505138/1‑101 (MQ=255)
                                                                     gATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACt                               >  2:6540193/1‑101 (MQ=255)
                                                                     gATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGGGAAGGCAAAGCAACt                               >  2:6693406/1‑101 (MQ=255)
                                                                       ttCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGt                             <  1:4642992/101‑1 (MQ=255)
                                                                            ggAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTa                        >  2:5806193/1‑101 (MQ=255)
                                                                             gAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTAc                       <  1:3704863/101‑1 (MQ=255)
                                                                             gAAGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTAc                       >  1:1326889/1‑101 (MQ=255)
                                                                              aaGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACt                      <  1:8401963/101‑1 (MQ=255)
                                                                               aGCTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTg                     <  2:3471155/101‑1 (MQ=255)
                                                                                 cTTCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGAt                   <  1:9154424/101‑1 (MQ=255)
                                                                                   tCGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCt                 <  1:999900/101‑1 (MQ=255)
                                                                                    cGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTa                <  2:6201810/101‑1 (MQ=255)
                                                                                    cGACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTa                <  2:7443298/101‑1 (MQ=255)
                                                                                     gACACGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAg               <  1:6767633/101‑1 (MQ=255)
                                                                                      acacGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGa              >  2:6343058/1‑101 (MQ=255)
                                                                                      acacGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGa              >  1:6961587/1‑101 (MQ=255)
                                                                                       cacGCGGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGat             <  1:483045/101‑1 (MQ=255)
                                                                                         cgcgGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGatat           <  1:4242067/101‑1 (MQ=255)
                                                                                          gcgGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGAtata          <  1:2835236/101‑1 (MQ=255)
                                                                                           cgGGCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATaa         >  2:6097054/1‑101 (MQ=255)
                                                                                            gggCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAAt        <  2:3960923/101‑1 (MQ=255)
                                                                                              gCTTTGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTc      <  1:5156254/101‑1 (MQ=255)
                                                                                                tttGAGGG.G.CCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCg    <  1:3327193/101‑1 (MQ=255)
                                                                                                  tGA.GG.GTCCAAAGTTCAAAGGCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGcc  <  2:952748/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
AATGTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.GG.G.CCAAAGTTCAAAGG.C.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCC  >  NC_014034/2135037‑2135233

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG