Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,135,154 +C 99.0% intergenic (‑148/+63) RCAP_rcc01974 ← / ← RCAP_rcc01975 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,135,1541.→C99.0% 4.5 ‑0.4 ‑1.2512intergenic (‑148/+63)RCAP_rcc01974/RCAP_rcc01975hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (248/259):  ref base (3/2):  total (251/261)
Fisher's exact test strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.88e-02

Only 100 of 573 total aligned reads displayed.

ACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.G.GG.CCAAAGTTCAAAGG.C.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGACAC  >  NC_014034/2135055‑2135249
                                                                                                       |                                                                                                 
aCTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAgg                                                                                                    >  1:4403286/1‑101 (MQ=255)
  ttATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGG.c.c                                                                                                >  1:4056733/1‑101 (MQ=255)
  ttATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGG.c.c                                                                                                >  2:5915154/1‑101 (MQ=255)
   tATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGG.c.cc                                                                                               >  2:4536418/1‑101 (MQ=255)
   tATGGAAAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGG.c.cc                                                                                               <  1:7002856/101‑1 (MQ=255)
    aggtgaaaGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGG.c.ccc                                                                                              <  1:220485/95‑2 (MQ=255)
     tGGTAAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGG.c.cccg                                                                                             <  1:6114454/101‑3 (MQ=255)
      gttCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGG.c.cccgc                                                                                            >  1:469724/3‑98 (MQ=255)
      ggTGAAGCCATTAAAGTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGG.c.cccgc                                                                                            >  1:8832222/1‑98 (MQ=255)
        tCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGG.c.ccctctt                                                                                          >  1:8952509/1‑96 (MQ=255)
         cAAGCCATTACATTGCGTTTATAGCAACTTTGCTTCCGCGTCGATTCCGGGAAGCTTAGAAACGAGGGCTTTGAGG.GG.CCAAAGTTCAAAGG.CACC.GCttt                                                                                         >  2:5617342/1‑101 (MQ=255)
         cAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCttt                                                                                         <  2:2818906/101‑1 (MQ=255)
          aaGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTc                                                                                        >  1:375547/1‑101 (MQ=255)
          aaGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGAGGGGTTTGAGG.GG.GCAAAGGTCAAAGG.c.cccagcttc                                                                                        >  2:4234163/1‑94 (MQ=255)
           aGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTcc                                                                                       <  1:7403217/101‑1 (MQ=255)
            gCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCa                                                                                      <  1:1573539/101‑1 (MQ=255)
             ccATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAg                                                                                     <  1:6412903/101‑1 (MQ=255)
              cATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGA.C.CCGGCTTTccggg                                                                                    >  2:8946775/1‑98 (MQ=255)
                ttAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCgg                                                                                  >  1:3118281/1‑101 (MQ=255)
                 tAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCggg                                                                                 >  2:6990817/1‑101 (MQ=255)
                 tAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCggg                                                                                 >  1:8655364/1‑101 (MQ=255)
                   aaTTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGc                                                                               >  2:7321218/1‑101 (MQ=255)
                   aaTTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGc                                                                               >  2:6592891/1‑101 (MQ=255)
                         gTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAggg                                                                         >  2:7914689/1‑101 (MQ=255)
                            tATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGcg                                                                      >  1:2439254/1‑101 (MQ=255)
                            tATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGcg                                                                      >  2:1618333/1‑101 (MQ=255)
                            tATCGCAACTTTACTTCCGCGTCGATTCATGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGcg                                                                      >  1:8084847/1‑101 (MQ=255)
                              tCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGcgac                                                                    <  1:1060444/101‑1 (MQ=255)
                                 cAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCgacgac                                                                 <  2:133791/101‑1 (MQ=255)
                                  aaCTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGacgacg                                                                >  2:106340/1‑101 (MQ=255)
                                  aaCTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGacgacg                                                                >  1:3471259/1‑101 (MQ=255)
                                    cTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGt                                                              >  1:1388670/1‑101 (MQ=255)
                                        gCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAg                                                          >  2:5312163/1‑101 (MQ=255)
                                          ttCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGc                                                        <  1:1229645/101‑1 (MQ=255)
                                           tCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCa                                                       <  2:1146771/101‑1 (MQ=255)
                                              gcgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAg                                                    >  2:3214842/1‑101 (MQ=255)
                                               cgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGc                                                   >  2:2090315/1‑101 (MQ=255)
                                                gTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCa                                                  >  1:7516803/1‑101 (MQ=255)
                                                gTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCa                                                  <  1:1921918/101‑1 (MQ=255)
                                                  cGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGCAGGCAAAGCAAc                                                >  1:9061058/1‑101 (MQ=255)
                                                   gATTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACt                                               <  2:2439181/101‑1 (MQ=255)
                                                    aTTCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTg                                              >  1:3010230/1‑101 (MQ=255)
                                                     ttCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGt                                             <  2:7688630/101‑1 (MQ=255)
                                                     ttCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGt                                             >  2:4165754/1‑101 (MQ=255)
                                                     ttCGTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGt                                             <  2:8291873/101‑1 (MQ=255)
                                                      tCGTGGAAGCTTCGACACGCGGGCTTTGA.GTGG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTc                                            <  2:9165337/101‑1 (MQ=255)
                                                        gTGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGc                                          >  1:5395284/1‑101 (MQ=255)
                                                         tGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCt                                         >  1:5246186/1‑101 (MQ=255)
                                                         tGGAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCt                                         <  2:255466/101‑1 (MQ=255)
                                                          ggAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTa                                        >  2:5806193/1‑101 (MQ=255)
                                                           gAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTAc                                       >  1:6782187/1‑101 (MQ=255)
                                                           gAAGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTAc                                       >  1:6486796/1‑101 (MQ=255)
                                                            aaGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACt                                      <  1:8401963/101‑1 (MQ=255)
                                                             aGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTg                                     <  1:5039458/101‑1 (MQ=255)
                                                             aGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTg                                     <  2:3471155/101‑1 (MQ=255)
                                                             aGCTTCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTg                                     >  2:39086/1‑101 (MQ=255)
                                                                ttCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATc                                  <  1:7661970/101‑1 (MQ=255)
                                                                ttCGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATc                                  >  2:9027726/1‑101 (MQ=255)
                                                                  cGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTa                                <  2:6201810/101‑1 (MQ=255)
                                                                  cGACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTa                                <  1:5912190/101‑1 (MQ=255)
                                                                   gACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAg                               <  1:4919273/101‑1 (MQ=255)
                                                                   gACACGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAg                               <  1:6847469/101‑1 (MQ=255)
                                                                    acacGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGa                              <  2:5112117/101‑1 (MQ=255)
                                                                    acacGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGa                              >  2:2656517/1‑101 (MQ=255)
                                                                      acGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGata                            <  2:4353130/101‑1 (MQ=255)
                                                                      acGCGGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGata                            <  1:716000/101‑1 (MQ=255)
                                                                        gcgGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGAtata                          <  1:2835236/101‑1 (MQ=255)
                                                                        gcgGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGAtata                          <  2:4785614/101‑1 (MQ=255)
                                                                         cgGGCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATaa                         >  2:6097054/1‑101 (MQ=255)
                                                                         cgGGCTTTGAGG.GG.CCAAAGATCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATaa                         >  1:8225797/1‑101 (MQ=255)
                                                                          gggCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAAt                        >  1:3653982/1‑101 (MQ=255)
                                                                          gggCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAAt                        <  2:8734001/101‑1 (MQ=255)
                                                                           ggCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAAtt                       <  1:4225930/101‑1 (MQ=255)
                                                                           ggCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTACAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAAtt                       >  2:8533693/1‑101 (MQ=255)
                                                                           ggCTTTGAGG.GG.CCAAAGTTCAAAGG.CTCC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAAtt                       <  2:6336870/101‑1 (MQ=255)
                                                                            gCTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTc                      >  2:3663596/1‑101 (MQ=255)
                                                                             cTTTGAGG.GG.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTcc                     <  2:4682113/101‑1 (MQ=255)
                                                                               ttgagg.gg.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTGCGc                   >  1:2919717/6‑101 (MQ=255)
                                                                                tgagg.gg.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGcc                  >  1:5664612/5‑101 (MQ=255)
                                                                                tgagg.gg.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGcc                  <  2:3118182/97‑1 (MQ=255)
                                                                                tgagg.gg.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGcc                  >  2:1940637/5‑101 (MQ=255)
                                                                                tgagg.gg.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGcc                  >  2:1752889/5‑101 (MQ=255)
                                                                                tGA.G.GGTCCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGcc                  <  2:952748/101‑1 (MQ=255)
                                                                                 gagg.gg.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCa                 <  1:9190079/98‑1 (MQ=255)
                                                                                 gagg.gg.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCa                 <  2:8154862/98‑1 (MQ=255)
                                                                                 gagg.gg.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCa                 >  2:7504664/4‑101 (MQ=255)
                                                                                  agg.gg.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCaa                >  1:7962240/3‑101 (MQ=255)
                                                                                  agg.gg.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCaa                >  2:7079980/3‑101 (MQ=255)
                                                                                  agg.gg.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGAGATACTTCCACCaa                >  2:6826050/3‑101 (MQ=255)
                                                                                    g.gg.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATg              <  2:5049311/101‑1 (MQ=255)
                                                                                      gg.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGt             <  2:4019331/101‑1 (MQ=255)
                                                                                       g.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGtt            <  1:388200/101‑1 (MQ=255)
                                                                                       g.CCAAAGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGtt            <  1:6693674/101‑1 (MQ=255)
                                                                                           aaaGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCt         <  1:7845256/101‑1 (MQ=255)
                                                                                             aGTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCg       <  1:4577406/101‑1 (MQ=255)
                                                                                              gTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCgg      >  2:6704256/1‑101 (MQ=255)
                                                                                              gTTCAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCgg      <  2:2270311/101‑1 (MQ=255)
                                                                                                 cAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGaca   <  1:2712751/101‑1 (MQ=255)
                                                                                                 cAAAGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGaca   <  1:4802187/101‑1 (MQ=255)
                                                                                                  aaaGGCC.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGacac  <  1:3275460/101‑1 (MQ=255)
                                                                                                       |                                                                                                 
ACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.G.GG.CCAAAGTTCAAAGG.C.CC.GCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGACAC  >  NC_014034/2135055‑2135249

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 32 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG