Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,165,863 +C 100% intergenic (‑168/‑216) RCAP_rcc02007 ← / → tig phage integrase/trigger factor

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,165,8631.→C100.0% 1565.3 417intergenic (‑168/‑216)RCAP_rcc02007/tigphage integrase/trigger factor
Reads supporting (aligned to +/- strand):  new base (234/181):  ref base (1/0):  total (235/182)

Only 100 of 462 total aligned reads displayed.

AATTACATCTTTGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTT.CTC.TG.CCTG.CCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGAAAA  >  NC_014034/2165763‑2165955
                                                                                                     |                                                                                               
aaTTACATCTTTGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCttt                                                                                                  >  1:1965516/1‑101 (MQ=255)
 aTTACATCTTTGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTt.c                                                                                                <  2:8550463/101‑1 (MQ=255)
  ttACATCTTTGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTt.cc                                                                                               >  1:6340289/1‑100 (MQ=255)
   tACATCTTTGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTt.cct                                                                                              <  1:4940826/101‑3 (MQ=255)
   tACATCTTTGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTt.cct                                                                                              >  2:77093/1‑99 (MQ=255)
    aCATCTTTGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTt.cctc                                                                                             >  1:627359/1‑98 (MQ=255)
    aCATCTTTGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTt.cctc                                                                                             >  2:6792180/1‑98 (MQ=255)
     cATCTTTGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTt.cctct                                                                                            <  1:1159576/101‑5 (MQ=255)
     cATCTTTGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTt.cctct                                                                                            >  1:6804720/1‑97 (MQ=255)
        cTTTGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTc.tg.cc                                                                                        >  1:8499755/1‑101 (MQ=255)
         tttGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTc.tg.ccc                                                                                       <  1:9165603/101‑2 (MQ=255)
         tttGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTc.tg.ccc                                                                                       >  2:6165052/1‑100 (MQ=255)
         tttGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTc.tg.ccc                                                                                       >  2:2559561/1‑100 (MQ=255)
           tGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTc.tg.ccctg                                                                                     >  2:5483613/1‑98 (MQ=255)
           tGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTc.tg.ccctg                                                                                     >  2:3039059/1‑98 (MQ=255)
           tGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTc.tg.ccctg                                                                                     <  2:4562816/101‑4 (MQ=255)
            gcggcgTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTc.tg.ccctgc                                                                                    <  1:4602456/101‑5 (MQ=255)
              ggcgTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTG.ccc                                                                                  <  1:2336525/101‑1 (MQ=255)
                cgTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTG.ccccg                                                                                >  2:3605308/1‑99 (MQ=255)
                 gTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTG.ccccgc                                                                               >  2:4992819/1‑98 (MQ=255)
                 gTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTG.ccccgc                                                                               >  1:8769204/1‑98 (MQ=255)
                  ttGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTG.ccccgcg                                                                              <  2:1628655/101‑5 (MQ=255)
                    gagaTTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCt                                                                             >  1:6974863/1‑101 (MQ=255)
                     agaTTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTg                                                                            >  2:1769486/1‑101 (MQ=255)
                     agaTTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTg                                                                            >  1:659721/1‑101 (MQ=255)
                           cccGGGGCGCAGAGTCAACCTGAGACGGCATGGAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGtct                                                                      >  1:1552989/1‑101 (MQ=255)
                            ccGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGtctc                                                                     <  1:5529833/101‑1 (MQ=255)
                                ggCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCgggg                                                                 <  1:1353764/101‑1 (MQ=255)
                                ggCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCgggg                                                                 >  2:3735991/1‑101 (MQ=255)
                                    cAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtc                                                             <  2:6976160/101‑1 (MQ=255)
                                      gagTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctg                                                           >  2:881380/1‑101 (MQ=255)
                                      gagTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctg                                                           >  1:1878386/1‑101 (MQ=255)
                                      gagTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctg                                                           >  1:5305773/1‑101 (MQ=255)
                                       agTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctgt                                                          <  2:3143277/101‑1 (MQ=255)
                                        gTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATATGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctgtc                                                         >  2:8283483/1‑101 (MQ=255)
                                         tCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctgtct                                                        <  2:1996825/101‑1 (MQ=255)
                                         tCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctgtct                                                        <  2:4194320/101‑1 (MQ=255)
                                          cAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGcagatcggaa                                                       >  1:5437652/1‑92 (MQ=255)
                                          cAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGtctgtctg                                                       >  1:7795366/1‑101 (MQ=255)
                                           aaCCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTgg                                                      >  2:7956521/1‑101 (MQ=255)
                                            aCCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTggg                                                     >  2:2427915/1‑101 (MQ=255)
                                            aCCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTggg                                                     >  1:5826822/1‑101 (MQ=255)
                                             ccTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGc                                                    <  2:7916051/101‑1 (MQ=255)
                                              cTGAGACGGCATGCAACACGGCACTGGTGCAGCCCCCCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGCCTCGGGGGGTCTGTCCGGCCg                                                   >  1:3918998/1‑101 (MQ=255)
                                               tGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGa                                                  >  1:8880287/1‑101 (MQ=255)
                                                gagaCGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGaa                                                 >  2:7015911/1‑101 (MQ=255)
                                                gagaCGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGaa                                                 <  2:575116/101‑1 (MQ=255)
                                                gagaCGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGaa                                                 >  1:170361/1‑101 (MQ=255)
                                                 agaCGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAAt                                                >  2:2777656/1‑101 (MQ=255)
                                                  gaCGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTCTTC.CCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATc                                               <  2:1334696/101‑1 (MQ=255)
                                                   aCGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCt                                              <  1:1047373/101‑1 (MQ=255)
                                                     ggCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCttt                                            <  1:838034/101‑1 (MQ=255)
                                                       cATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTc                                          <  2:9096230/101‑1 (MQ=255)
                                                       cATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTc                                          >  1:4441842/1‑101 (MQ=255)
                                                        aTGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCg                                         <  1:3441945/101‑1 (MQ=255)
                                                        aTGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCg                                         <  1:43185/101‑1 (MQ=255)
                                                        aTGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCg                                         <  2:3195733/101‑1 (MQ=255)
                                                         tGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGa                                        >  2:1124476/1‑101 (MQ=255)
                                                         tGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGCCTGTCTGGGCGAATCTTTTCGa                                        >  2:5558132/1‑101 (MQ=255)
                                                          gCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGAt                                       >  1:2577086/1‑101 (MQ=255)
                                                            aaCACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCt                                     <  1:7646427/101‑1 (MQ=255)
                                                             acacGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCtt                                    <  1:5926876/101‑1 (MQ=255)
                                                              cacGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCttt                                   >  1:4171033/1‑101 (MQ=255)
                                                               acGGCGCTGGTGCAGACCACCCGCACCATCNGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTc                                  <  1:2833602/101‑1 (MQ=255)
                                                               acGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTc                                  <  2:5106581/101‑1 (MQ=255)
                                                                cGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCt                                 >  2:4738487/1‑101 (MQ=255)
                                                                cGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCt                                 <  1:2435405/101‑1 (MQ=255)
                                                                  gCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGc                               >  1:3209218/1‑101 (MQ=255)
                                                                  gCACTGGTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGc                               >  2:5332086/1‑101 (MQ=255)
                                                                        gTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGcc                         >  2:6500687/1‑101 (MQ=255)
                                                                        gTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGcc                         >  1:4784780/1‑101 (MQ=255)
                                                                        gTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGcc                         >  1:6026773/1‑101 (MQ=255)
                                                                        gTGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGcc                         >  2:7192917/1‑101 (MQ=255)
                                                                         tGCAGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGccc                        <  2:6026536/101‑2 (MQ=255)
                                                                            aGACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGa                     >  1:3104824/1‑101 (MQ=255)
                                                                             gACCACCCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGAc                    <  1:7131650/101‑1 (MQ=255)
                                                                              accaccCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGAcc                   >  2:7414149/1‑101 (MQ=255)
                                                                              accaccCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGAcc                   <  1:371372/101‑1 (MQ=255)
                                                                              accaccCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGAcc                   >  1:7280706/1‑101 (MQ=255)
                                                                              accaccCGCACCATCTGACCTTTCCTC.TGACCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGAcc                   >  2:3932206/1‑101 (MQ=255)
                                                                               ccaccCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCg                  >  1:8265681/1‑101 (MQ=255)
                                                                               ccaccCGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCg                  >  1:6063449/1‑101 (MQ=255)
                                                                                   ccGCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGaaaa              <  2:4311663/101‑1 (MQ=255)
                                                                                   ccGCACCATCTGACCTTTCCTC.AGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGaaaa              >  2:815014/1‑101 (MQ=255)
                                                                                     gCACCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACg            >  2:3372151/1‑101 (MQ=255)
                                                                                      cACCATCTGACCGTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACgg           >  1:8072222/1‑101 (MQ=255)
                                                                                       cccATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGc          <  2:8438495/100‑1 (MQ=255)
                                                                                       aCCATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGc          <  2:6340040/101‑1 (MQ=255)
                                                                                        ccATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGcc         >  1:7974755/1‑101 (MQ=255)
                                                                                        ccATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGcc         <  1:7492153/101‑1 (MQ=255)
                                                                                         cATCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGAGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGccc        >  2:4454427/1‑101 (MQ=255)
                                                                                          aTCTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCg       <  1:8674446/101‑1 (MQ=255)
                                                                                            cTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGa     >  2:1891774/1‑101 (MQ=255)
                                                                                            cTGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCCGa     >  1:4532690/1‑101 (MQ=255)
                                                                                             tGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGaa    >  2:2209229/1‑101 (MQ=255)
                                                                                             tGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGaa    >  2:607786/1‑101 (MQ=255)
                                                                                             tGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGaa    >  1:6874231/1‑101 (MQ=255)
                                                                                             tGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGaa    <  1:5126376/101‑1 (MQ=255)
                                                                                             tGACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCCTTCTGCCGCGCCGGGACCGAAAACGGCCCGGaa    <  1:6444446/101‑1 (MQ=255)
                                                                                              gACCTTTCCTC.TGCCCTGCCCCGCGCTGCCGTCGCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGaaa   <  2:7300568/101‑1 (MQ=255)
                                                                                                     |                                                                                               
AATTACATCTTTGCGGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTT.CTC.TG.CCTG.CCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGAAAA  >  NC_014034/2165763‑2165955

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG