Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,276 +T 98.8% coding (101/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2762.→T98.8% 2.7 ‑0.4 ‑0.7334coding (101/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (190/139):  ref base (3/1):  total (194/140)
Fisher's exact test strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.83e-01

Only 100 of 367 total aligned reads displayed.

CATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AA...GGGTT.G..GTCGGT.CTT.G.TGTACAGACAGGCCCCGGTTTCGGCC.G.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCC  >  NC_014034/2177181‑2177363
                                                                                                     |                                                                                               
caTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.g..gtg                                                                                             >  2:5036516/1‑100 (MQ=255)
caTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.g..gtg                                                                                             <  1:8569903/101‑2 (MQ=255)
  tCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.g..gtgtc                                                                                           <  2:8763681/101‑4 (MQ=255)
    gCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.g..gtgtcgg                                                                                         >  2:6203201/1‑96 (MQ=255)
     ccATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.g..gtgtcggt                                                                                        <  2:2280541/101‑7 (MQ=255)
      cATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.g..gtgtcggtc                                                                                       >  1:4944901/1‑94 (MQ=255)
       aTCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.g..gtgtcggtct                                                                                      <  2:6900387/101‑9 (MQ=255)
        tCGATCTTGCCCGCATAGGTCGATGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.g..gtgtcggtctt                                                                                     <  2:7723292/101‑10 (MQ=255)
        tCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.g..gtgtcggtctt                                                                                     <  1:7834948/101‑10 (MQ=255)
        tCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.g..gtgtcggtctt                                                                                     <  2:6160973/101‑10 (MQ=255)
         cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTt.g                                                                                    >  2:2687142/1‑101 (MQ=255)
         cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTt.g                                                                                    >  1:1677128/1‑101 (MQ=255)
           aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTt.ggg                                                                                  >  2:3734140/1‑99 (MQ=255)
              ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTt.ggtgat                                                                               <  1:6208218/101‑6 (MQ=255)
              ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTt.ggtgat                                                                               >  2:7295895/1‑96 (MQ=255)
              ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTt.ggtgat                                                                               >  2:797040/1‑96 (MQ=255)
               tGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTt.ggtgatc                                                                              <  2:5978185/101‑7 (MQ=255)
                gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTt.ggtgatca                                                                             >  2:4770174/1‑94 (MQ=255)
                 cccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTt.ggtgaccag                                                                            <  1:7206723/101‑9 (MQ=255)
                  ccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTt.ggtgatcaga                                                                           <  1:2413288/101‑10 (MQ=255)
                   cGCAGAGGTCGAGAGCACCAGCCCCATCGCGGTCGCCTTTGCCGAAGCACCTGCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTt.ggtgatcagac                                                                          <  2:805421/101‑11 (MQ=255)
                     cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGAtcagacag                                                                        >  2:2357363/1‑101 (MQ=255)
                      aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAgg                                                                       <  1:1157299/101‑1 (MQ=255)
                      aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAgg                                                                       >  1:58927/1‑101 (MQ=255)
                       tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGc                                                                      >  1:3262191/1‑101 (MQ=255)
                        aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGcc                                                                     <  2:5283263/101‑1 (MQ=255)
                        aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGcc                                                                     <  2:320861/101‑1 (MQ=255)
                        aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGcc                                                                     <  2:1015465/101‑1 (MQ=255)
                         ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGccc                                                                    >  1:2466862/1‑101 (MQ=255)
                         ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGccc                                                                    >  2:4162952/1‑101 (MQ=255)
                         ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGcac                                                                    >  1:9020604/1‑99 (MQ=255)
                          gTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGcccc                                                                   <  1:836497/101‑1 (MQ=255)
                           tCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCg                                                                  >  2:3039489/1‑101 (MQ=255)
                            cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCgg                                                                 >  1:3995306/1‑101 (MQ=255)
                              agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTCCTTCTTGCGGAA.AACG.GGGTTGG..GGTGGTCCTT.GTTGTTCAGAAAGGCCCCGGtt                                                               >  1:5812359/4‑101 (MQ=255)
                                 gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCg                                                            <  2:1785599/101‑1 (MQ=255)
                                  cACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCgg                                                           >  1:6025308/1‑101 (MQ=255)
                                  cACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCgg                                                           >  2:6788361/1‑101 (MQ=255)
                                    ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGcc                                                         >  2:2491590/1‑101 (MQ=255)
                                    ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGcc                                                         >  2:4527118/1‑101 (MQ=255)
                                     cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.g                                                       >  2:739596/1‑101 (MQ=255)
                                     cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.g                                                       >  1:6120399/1‑101 (MQ=255)
                                      aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.G.a                                                     >  2:7647441/1‑101 (MQ=255)
                                        ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.G.aag                                                   <  1:2580809/101‑3 (MQ=255)
                                         cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.G.aagc                                                  <  1:315735/101‑4 (MQ=255)
                                           cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.G.aagccg                                                >  2:14314/1‑96 (MQ=255)
                                            aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGATGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAgccgc                                                >  2:8206430/1‑101 (MQ=255)
                                            aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAgccgc                                                <  1:7714733/101‑1 (MQ=255)
                                              ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGGTG.GT.GTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGccc                                              >  2:5624944/1‑101 (MQ=255)
                                                cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGTCCCCGGTTTCGGCC.GAAGCCGCCCCg                                            <  2:4073265/101‑1 (MQ=255)
                                                cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCg                                            >  1:7084828/1‑101 (MQ=255)
                                                 ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCgg                                           >  2:7083056/1‑101 (MQ=255)
                                                  gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGc                                          <  1:8845624/101‑1 (MQ=255)
                                                  gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGc                                          >  1:1465149/1‑101 (MQ=255)
                                                  gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGc                                          >  2:2907909/1‑101 (MQ=255)
                                                   tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGcc                                         <  2:9131493/101‑1 (MQ=255)
                                                   tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGcc                                         <  1:1021478/101‑1 (MQ=255)
                                                   gcGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGcc                                         <  2:519833/100‑1 (MQ=255)
                                                    cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCa                                        <  2:5714299/101‑1 (MQ=255)
                                                     gCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAg                                       >  1:5085175/1‑101 (MQ=255)
                                                     gCCTGTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGGTG.GT.GTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAg                                       <  1:560592/101‑1 (MQ=255)
                                                       cTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAgcg                                     <  1:52318/101‑1 (MQ=255)
                                                       cTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAgcg                                     >  1:4210195/1‑101 (MQ=255)
                                                        tttGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAgcgc                                    >  1:3531666/1‑101 (MQ=255)
                                                          tGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCgg                                  >  2:3317957/1‑101 (MQ=255)
                                                            ccGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGaa                                <  1:2141276/101‑1 (MQ=255)
                                                            ccGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGaa                                <  1:3466440/101‑1 (MQ=255)
                                                            ccGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGaa                                >  1:460224/1‑101 (MQ=255)
                                                             cGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGaaa                               >  2:1880723/1‑101 (MQ=255)
                                                             cGAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGaaa                               >  2:8374679/1‑101 (MQ=255)
                                                              gAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGaaaa                              >  1:5147831/1‑101 (MQ=255)
                                                              gAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGaaaa                              <  1:4354480/101‑1 (MQ=255)
                                                              gAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGaaaa                              >  1:8151568/1‑101 (MQ=255)
                                                              gAAGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGaaaa                              >  2:113610/1‑101 (MQ=255)
                                                               aaGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAAc                             >  2:6484777/1‑101 (MQ=255)
                                                               aaGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAAc                             >  2:6338451/1‑101 (MQ=255)
                                                               aaGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAAc                             <  2:388257/101‑1 (MQ=255)
                                                                aGCACCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACg                            >  2:3002774/1‑101 (MQ=255)
                                                                   aCCTTCCTTCATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCAGCCCCGGCCAGCGCGGAAAACGCCa                         >  2:5365671/1‑101 (MQ=255)
                                                                     cttccttcATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                      >  2:5431504/1‑101 (MQ=255)
                                                                       tccttcATCTTGGCGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                    <  2:7168831/101‑3 (MQ=255)
                                                                       tccttcATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                    >  2:4593348/1‑99 (MQ=255)
                                                                       tccttcATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                    >  1:1755987/1‑99 (MQ=255)
                                                                       tccttcATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                    <  1:2908007/101‑3 (MQ=255)
                                                                        ccttcATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                   <  2:6596678/101‑4 (MQ=255)
                                                                        ccttcATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                   >  1:2815683/1‑98 (MQ=255)
                                                                        ccttcATCTTGACGAA.AAAG.GGGTT.GGCGTCGGT.CTTGG.TGATCAGACAGCCCCCGGTTTCGGCC.GAAGCCGCCACGGCCCGCGCGGAAAACGCCA.gggca                   >  1:4445495/1‑98 (MQ=255)
                                                                         cttcATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                  >  1:2692692/1‑97 (MQ=255)
                                                                         cttcATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                  >  1:3077793/1‑97 (MQ=255)
                                                                          ttcATCTTGACGAA.AAGG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                 >  2:3623132/1‑96 (MQ=255)
                                                                          ttcATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                 >  1:6386594/1‑96 (MQ=255)
                                                                           tcATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                 >  2:3446852/1‑101 (MQ=255)
                                                                           tcATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                 >  2:8972124/1‑101 (MQ=255)
                                                                            cATCTTGACGAACAAG..GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCCGG.AGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                >  1:6562830/1‑101 (MQ=255)
                                                                            cATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                >  2:6712324/1‑101 (MQ=255)
                                                                            cATCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                <  2:5875986/101‑1 (MQ=255)
                                                                              tCTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc             <  1:7450325/101‑1 (MQ=255)
                                                                               cTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc            >  2:746267/1‑101 (MQ=255)
                                                                               cTTGACGAA.AAAG.GGGTT.GGTGTCGGT.CTTGG.TGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc            <  1:6736219/101‑1 (MQ=255)
                                                                                           acgggttggg..gtcggt.cTTGG.TGAGCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc  <  2:4259834/92‑1 (MQ=255)
                                                                                                     |                                                                                               
CATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AA...GGGTT.G..GTCGGT.CTT.G.TGTACAGACAGGCCCCGGTTTCGGCC.G.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCC  >  NC_014034/2177181‑2177363

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG