Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,285 +G 100% coding (92/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2851.→G100.0% 1397.4 364coding (92/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (209/155):  ref base (0/0):  total (209/155)

Only 100 of 410 total aligned reads displayed.

CGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA...GGGTTG..GT..CGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATC  >  NC_014034/2177190‑2177377
                                                                                                       |                                                                                                
cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTt.g                                                                                                 >  1:1677128/1‑101 (MQ=255)
cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTt.g                                                                                                 >  2:279337/1‑101 (MQ=255)
    cTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTt.ggtga                                                                                             >  1:9196475/1‑97 (MQ=255)
     ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTt.ggtgat                                                                                            >  2:7295895/1‑96 (MQ=255)
     ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTt.ggtgat                                                                                            <  1:6208218/101‑6 (MQ=255)
      tGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTt.ggtgatc                                                                                           <  2:819410/101‑7 (MQ=255)
      tGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTt.ggtgatc                                                                                           <  2:5978185/101‑7 (MQ=255)
      tGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTt.ggtgatc                                                                                           <  1:7389930/101‑7 (MQ=255)
         ccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTt.ggtgatcaga                                                                                        <  1:2413288/101‑10 (MQ=255)
            cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGAtcagacag                                                                                      <  1:7477561/101‑1 (MQ=255)
            cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGAtcagacag                                                                                      >  2:6582610/1‑101 (MQ=255)
             aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAgg                                                                                     >  1:58927/1‑101 (MQ=255)
             aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAgg                                                                                     <  1:1157299/101‑1 (MQ=255)
                ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGccc                                                                                  >  1:2466862/1‑101 (MQ=255)
                ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGccc                                                                                  >  2:4162952/1‑101 (MQ=255)
                ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGcac                                                                                  >  1:9020604/1‑99 (MQ=255)
                 gTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGcccc                                                                                 <  2:8977281/101‑1 (MQ=255)
                  tCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCg                                                                                >  1:6113390/1‑101 (MQ=255)
                   cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCgg                                                                               >  2:8005793/1‑101 (MQ=255)
                    gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGt                                                                              <  1:5036684/101‑1 (MQ=255)
                    gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGt                                                                              >  2:5710329/1‑101 (MQ=255)
                    gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTG..GTGGCGGTCTTGGTGATCAGACAGGCCCCGGt                                                                              >  1:6391745/1‑101 (MQ=255)
                      gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGttt                                                                            <  2:7630381/99‑1 (MQ=255)
                      gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGttt                                                                            >  2:303840/3‑101 (MQ=255)
                        gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCg                                                                          <  2:3488861/101‑1 (MQ=255)
                        gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCg                                                                          >  2:244410/1‑101 (MQ=255)
                         cACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCgg                                                                         <  2:139451/101‑1 (MQ=255)
                         cACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCgg                                                                         <  2:4024326/101‑1 (MQ=255)
                         cACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCgg                                                                         >  1:8013640/1‑101 (MQ=255)
                          aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGc                                                                        <  2:5063242/101‑1 (MQ=255)
                            cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                                                      >  2:739596/1‑101 (MQ=255)
                             aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.a                                                                    <  2:2574966/101‑1 (MQ=255)
                              gCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aa                                                                   <  2:6623425/101‑2 (MQ=255)
                                  cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagccg                                                               >  1:8525738/1‑96 (MQ=255)
                                  cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagccg                                                               >  2:3126977/1‑96 (MQ=255)
                                   aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAgccgc                                                               <  1:7714733/101‑1 (MQ=255)
                                    tctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAgccgcc                                                              <  1:6785106/101‑1 (MQ=255)
                                     ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                             <  2:5554347/101‑1 (MQ=255)
                                     ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                             >  1:8960230/1‑101 (MQ=255)
                                     ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                             >  2:7704397/1‑101 (MQ=255)
                                      ttggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                                            <  2:3328538/99‑1 (MQ=255)
                                       cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                                           >  2:8426951/1‑101 (MQ=255)
                                         gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGc                                                         >  1:1465149/1‑101 (MQ=255)
                                         gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGc                                                         >  1:5791755/1‑101 (MQ=255)
                                          tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                        <  1:1021478/101‑1 (MQ=255)
                                          tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                        <  2:7117621/101‑1 (MQ=255)
                                          gcGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                        <  2:519833/100‑1 (MQ=255)
                                            gCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                                                      <  1:3479900/101‑1 (MQ=255)
                                            gCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                                                      <  1:2687224/101‑1 (MQ=255)
                                            gCCTGTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGGTGGT.GT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                                                      <  1:560592/101‑1 (MQ=255)
                                              cTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcg                                                    >  2:4587255/1‑101 (MQ=255)
                                               tttGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                                                   >  1:437215/1‑101 (MQ=255)
                                               tttGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                                                   <  1:3801231/101‑1 (MQ=255)
                                                 tGCCGAAGCACCTTCCTTCATCTTGGCGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                                                 >  2:6744259/1‑101 (MQ=255)
                                                  gCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                                                >  1:5952372/1‑101 (MQ=255)
                                                   ccGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                               >  1:460224/1‑101 (MQ=255)
                                                   ccGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                               <  1:2141276/101‑1 (MQ=255)
                                                   ccGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                               <  1:6619669/101‑1 (MQ=255)
                                                    cGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                              >  2:1880723/1‑101 (MQ=255)
                                                     gAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGataa                                             >  2:9103627/1‑101 (MQ=255)
                                                     gAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaac                                             >  2:448216/1‑100 (MQ=255)
                                                     gAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                             <  2:5309471/101‑1 (MQ=255)
                                                      aaGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                            >  1:4541180/1‑101 (MQ=255)
                                                      aaGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                            >  2:6338451/1‑101 (MQ=255)
                                                      aaGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                            <  2:388257/101‑1 (MQ=255)
                                                       aGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACg                                           >  2:467944/1‑101 (MQ=255)
                                                       aGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACg                                           >  1:8787805/1‑101 (MQ=255)
                                                        gCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                                          >  2:6849300/1‑101 (MQ=255)
                                                          cccttccttCATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                        <  1:3314693/100‑1 (MQ=255)
                                                            cttccttcATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                     <  2:8325012/101‑1 (MQ=255)
                                                             ttccttcATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGACCAGCGCGGAAAACGCCA.ggg                                    >  2:5402422/1‑100 (MQ=255)
                                                              tccttcATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                   <  1:2908007/101‑3 (MQ=255)
                                                               ccttcATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                                  >  1:2815683/1‑98 (MQ=255)
                                                               ccttcATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                                  >  1:3179979/1‑98 (MQ=255)
                                                               ccttcATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                                  >  1:5105638/1‑98 (MQ=255)
                                                                cttcATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                                 >  1:3077793/1‑97 (MQ=255)
                                                                  tcATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                <  2:2980233/101‑1 (MQ=255)
                                                                  tcATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                >  2:7071974/1‑101 (MQ=255)
                                                                  tcATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                >  1:8223526/1‑101 (MQ=255)
                                                                   cATCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                               <  2:5875986/101‑1 (MQ=255)
                                                                     tCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                            <  2:8775108/101‑1 (MQ=255)
                                                                     tCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                            <  1:5224667/101‑1 (MQ=255)
                                                                     tCTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                            >  2:3722467/1‑101 (MQ=255)
                                                                      cTTGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.acc                           >  1:4219721/1‑101 (MQ=255)
                                                                       ttGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cccc                          >  1:401383/1‑101 (MQ=255)
                                                                       ttGACGAAAAAG.GGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cccc                          >  2:6163362/1‑101 (MQ=255)
                                                                                acgggttggg..gt..cggtcTTGGTGAGCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc                 <  2:4259834/92‑1 (MQ=255)
                                                                                   ggggttggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa                >  2:56398/10‑101 (MQ=255)
                                                                                   ggggttggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa                <  1:1958300/92‑1 (MQ=255)
                                                                                    gggttggtgt..cggtcTTGGTGATCAGACAGACCCCGGTTTCGGCCGAAGCTGCCCCGGCCAGCGCGGGAAACGCCAGGGAAGAGGCCCCCAACACGGCCAg               >  2:1742340/9‑101 (MQ=255)
                                                                                     ggttggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t             <  2:6397343/94‑1 (MQ=255)
                                                                                      gttggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGCAAACGCCAGGGCAGAGGCCCCCAACCGGGCCCG.tt            >  1:2529788/7‑101 (MQ=255)
                                                                                      gttggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt            <  1:7123026/95‑1 (MQ=255)
                                                                                       ttggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.ttt           <  2:1031674/96‑2 (MQ=255)
                                                                                        tggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg          <  2:1245857/97‑3 (MQ=255)
                                                                                         ggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc         <  2:7851899/98‑4 (MQ=255)
                                                                                          gtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg        <  2:2321313/99‑5 (MQ=255)
                                                                                           tgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc       >  2:3797626/2‑96 (MQ=255)
                                                                                            gt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgcn      >  1:6529607/1‑95 (MQ=255)
                                                                                                cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc     <  2:3269050/101‑1 (MQ=255)
                                                                                                       |                                                                                                
CGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA...GGGTTG..GT..CGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATC  >  NC_014034/2177190‑2177377

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG